Research Topics
| S J VesperSummaryAffiliation: U.S. Environmental Protection Agency Country: USA Publications
| Collaborators
|
Detail Information
Publications
Correlation between ERMI values and other moisture and mold assessments of homes in the American Healthy Homes SurveyStephen Vesper
United States Environmental Protection Agency, Cincinnati, OH, USA
J Urban Health 86:850-60. 2009..An additional objective was to compare the ERMI values in inner city east-Baltimore homes, where childhood asthma is common, to the AHHS randomly selected homes...- Hemolysis, toxicity, and randomly amplified polymorphic DNA analysis of Stachybotrys chartarum strainsS J Vesper
National Exposure Research Laboratory, U S Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Appl Environ Microbiol 65:3175-81. 1999..Only 3 of the 28 strains examined both were consistently hemolytic and produced highly toxic conidia. Each of these strains was isolated from a house in Cleveland where an infant had idiopathic pulmonary hemorrhage... - Specific molds associated with asthma in water-damaged homesStephen J Vesper
U S Environmental Protection Agency, Cincinnati, Ohio 45268, USA
J Occup Environ Med 48:852-8. 2006..We sought to determine if specific molds were found in significantly higher concentrations in the water-damaged homes of asthmatic children compared with homes with no visible water damage... - Relative moldiness index as predictor of childhood respiratory illnessStephen J Vesper
US Environmental Protection Agency, Cincinnati, OH, USA
J Expo Sci Environ Epidemiol 17:88-94. 2007..27). Conversely, a method developed and validated in this paper, using the RMI index fit to a logistic function, can be used to predict the occurrence of illness in homes and allows stake-holders the choice among various levels of risk... - Geographic distribution of Environmental Relative Moldiness Index molds in USA homesStephen Vesper
National Exposure Research Laboratory, United States Environmental Protection Agency, Cincinnati, OH 45268, USA
J Environ Public Health 2011:242457. 2011..The objective of this study was to quantify and describe the distribution of the 36 molds that make up the Environmental Relative Moldiness Index (ERMI)... - Quantitative PCR analysis of molds in the dust from homes of asthmatic children in North CarolinaStephen Vesper
National Exposure Research Laboratory NERL, United States US Environmental Protection Agency, 26 West M L King Ave, M L 314, Cincinnati, 45268, Ohio, USA
J Environ Monit 9:826-30. 2007..02 for each). Vacuum bag dust analysis may be a useful method for estimating the mold burden in a home... - Development of an Environmental Relative Moldiness index for US homesStephen Vesper
United States Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio 45268, USA
J Occup Environ Med 49:829-33. 2007..The objective of this study was to establish a national relative moldiness index for homes in the United States... - Opportunistic Aspergillus pathogens measured in home and hospital tap water by quantitative PCR (QPCR)S J Vesper
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, OH 45216, USA
J Water Health 5:427-31. 2007..Obtaining pathogen occurrence data in a timely manner could promote the elimination of the pathogens from the water supply of immunocompromised patients... - Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA)Stephen Vesper
National Exposure Research Laboratory, United States Environmental Protection Agency, 26 West Martin Luther King Ave, Cincinnati, OH 45268, USA
J Microbiol Methods 72:180-4. 2008.... - Higher Environmental Relative Moldiness Index (ERMIsm) values measured in Detroit homes of severely asthmatic childrenStephen Vesper
National Exposure Research Laboratory NERL, United States Environmental Protection Agency, Cincinnati, OH 45268, USA
Sci Total Environ 394:192-6. 2008..05; Wilcoxon Rank-sum test). The determination of the home's ERMI values may aid in prioritizing home remediation efforts, particularly in those children who are at increased risk for asthma exacerbation... - Mold species in dust from the International Space Station identified and quantified by mold-specific quantitative PCRStephen J Vesper
National Exposure Research Laboratory NERL, United States US Environmental Protection Agency, 26 West M L King Avenue, M L 314, Cincinnati, OH 45268, USA
Res Microbiol 159:432-5. 2008..No cells of the potential opportunistic pathogens Aspergillus fumigatus, Aspergillus terreus, Fusarium solani or Candida albicans were detected... - Screening tools to estimate mold burdens in homesStephen Vesper
United States Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio, USA
J Occup Environ Med 51:80-6. 2009..The objective of this study was to develop screening tools that could be used to estimate the mold burden in a home which would indicate whether more detailed testing might be useful... - Traditional mould analysis compared to a DNA-based method of mould analysisStephen Vesper
United States Environmental Protection Agency, Cincinnati OH, USA
Crit Rev Microbiol 37:15-24. 2011..Based on a national sampling and analysis by MSQPCR of dust in US homes, a scale for comparing the mould burden in homes was created called the Environmental Relative Mouldiness Index (ERMI)... - Comparison of populations of mould species in homes in the UK and USA using mould-specific quantitative PCRS J Vesper
US Environmental Protection Agency, Cincinnati, OH 45268, USA
Lett Appl Microbiol 41:367-73. 2005..To compare the populations of 81 mould species in homes in the USA and UK using mould-specific quantitative polymerase chain reaction (MSQPCR) technology... - Quantitative polymerase chain reaction analysis of fungi in dust from homes of infants who developed idiopathic pulmonary hemorrhagingStephen J Vesper
US Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio 45268, USA
J Occup Environ Med 46:596-601. 2004..The same 82 fungal species were also tested for hemolysin production on sheep's blood agar (incubated at 37 degree C). Hemolysins were more commonly produced by group I species (42%) compared with group II species (10%)... - Stachylysin may be a cause of hemorrhaging in humans exposed to Stachybotrys chartarumStephen J Vesper
National Exposure Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Infect Immun 70:2065-9. 2002..These results support the hypothesis that stachylysin may be one agent responsible for hemorrhaging in humans... - Evaluation of Stachybotrys chartarum in the house of an infant with pulmonary hemorrhage: quantitative assessment before, during, and after remediationS Vesper
US Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, OH 45268, USA
J Urban Health 77:68-85. 2000..DNA banding patterns suggested that at least one of these isolates was related to isolates from homes of infants with previously investigated cases... - Predicting virulence of Aeromonas isolates based on changes in transcription of c-jun and c-fos in human tissue culture cellsS L Hayes
USEPA, National Risk Management Research Laboratory, Water Supply Water Resources Division, Cincinnati, OH 45268, USA
J Appl Microbiol 107:964-9. 2009..To screen for the virulence potential of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2... - Quantification of siderophore and hemolysin from Stachybotrys chartarum strains, including a strain isolated from the lung of a child with pulmonary hemorrhage and hemosiderosisS J Vesper
National Environmental Research Laboratory, U S Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Appl Environ Microbiol 66:2678-81. 2000..chartarum isolated from case (n = 5) or control (n = 5) homes in Cleveland were analyzed for hemolytic activity, siderophore production, and relatedness as measured by random amplified polymorphic DNA analysis... - Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqMan(TM)fluorogenic probe systemR A Haugland
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, 45268
Mol Cell Probes 13:329-40. 1999..The method showed a similar level of agreement with direct counting in the quantification of S. chartarum conidia in air samples collected from several contaminated homes... - Quantitative PCR analysis of house dust can reveal abnormal mold conditionsTeija Meklin
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, OH 45268, USA
J Environ Monit 6:615-20. 2004..Culturing underestimated the concentrations of these four species by 2 to 3 orders of magnitude compared to QPCR... - Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysisRichard A Haugland
National Exposure Research Laboratory, U S Environmental Protection Agency, 26 W M L King Drive, Cincinnati, OH 45268, USA
J Microbiol Methods 50:319-23. 2002..However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure... - Human serum IgE reacts with a Metarhizium anisopliae fungal catalaseMarsha D W Ward
United States Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle Park, NC 27711, USA
Int Arch Allergy Immunol 150:343-51. 2009..anisopliae mycelia extract (MYC) proteins that are recognized by mouse serum IgE, (2) to determine if human serum IgE reacts with these proteins, and (3) to determine if these IgE-reactive proteins are found in other fungi... - Quantitative PCR analysis of selected Aspergillus, Penicillium and Paecilomyces speciesRichard A Haugland
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Syst Appl Microbiol 27:198-210. 2004.... - Aeromonas caviae strain induces Th1 cytokine response in mouse intestinal tractSamuel L Hayes
United States Environmental Protection Agency, National Risk Management Research Laboratory, Water Supply Water Resources Division, 26 W Martin Luther King Dr, MS 387, Cincinnati, OH 45268, USA
Can J Microbiol 56:27-31. 2010..This response could explain why a number of diarrheal waterborne disease cases have been attributed to A. caviae even though it lacks obvious enteropathogenic properties... - Characterization of Aeromonas virulence using an immunocompromised mouse modelDennis J Lye
National Exposure Research Laboratory, Microbial and Chemical Exposure Assessment Research Division, United States Environmental Protection Agency, Cincinnati, OH 45268, USA
Curr Microbiol 54:195-8. 2007..There were no correlations between the presence or absence of biochemical test results for putative virulence factors and persistence of the isolate in spleen and liver tissue at 24 hours... - Identification by microarray of a common pattern of gene expression in intact intestine and cultured intestinal cells exposed to virulent Aeromonas hydrophila isolatesSamuel L Hayes
USEPA, National Risk Management Research Laboratory, Water Supply Water Resources Division, Cincinnati, OH 45268, USA
J Water Health 4:381-8. 2006..Twenty-six genes were found to be in common between the two models, of which the majority are associated with the innate immune response... - Dose-dependent allergic responses to an extract of Penicillium chrysogenum in BALB/c miceYong Joo Chung
Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA
Toxicology 209:77-89. 2005..Taken together, repeated pulmonary challenge with P. chrysogenum extract induced dose-dependent allergic asthma-like responses in mice... - Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in waterNichole E Brinkman
National Exposure Research Laboratory, U.S. Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA
Appl Environ Microbiol 69:1775-82. 2003.... - Rapid monitoring by quantitative polymerase chain reaction for pathogenic Aspergillus during carpet removal from a hospitalAlice N Neely
Shriners Burns Hospital, Cincinnati, OH 45229-3095, USA
Infect Control Hosp Epidemiol 25:350-2. 2004.... - Evaluating virulence of waterborne and clinical Aeromonas isolates using gene expression and mortality in neonatal mice followed by assessing cell culture's ability to predict virulence based on transcriptional responseS L Hayes
USEPA, National Risk Management Research Laboratory, Water Supply Water Resources Division, Cincinnati, OH 45268, USA
J Appl Microbiol 103:811-20. 2007..To assess the virulence of Aeromonas spp. using two models, a neonatal mouse assay and a mouse intestinal cell culture... - Possible role of fungal hemolysins in sick building syndromeStephen J Vesper
U.S. Environmental Protection Agency Office of Research and Development, National Exposure Research Laboratory 26 W. M. L. King Drive Cincinnati, Ohio 45268, USA
Adv Appl Microbiol 55:191-213. 2004 - An extract of Stachybotrys chartarum causes allergic asthma-like responses in a BALB/c mouse modelMichael E Viana
Department of Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
Toxicol Sci 70:98-109. 2002..However, BSA was nonallergenic and did not generate respiratory physiological responses when administered by aspiration... - ELISA measurement of stachylysin in serum to quantify human exposures to the indoor mold Stachybotrys chartarumJeanette M Van Emon
United States Environmental Protection Agency (US EPA, Las Vegas, Nevada, USA
J Occup Environ Med 45:582-91. 2003..Stachylysin was also quantified in spore, wallboard, mycelial, and dust samples. The measurement of stachylysin may be a useful indicator in assessing human exposure to S. chartarum and in determining the presence of this indoor mold... - Comparison of mold concentrations quantified by MSQPCR in indoor and outdoor air sampled simultaneouslyTeija Meklin
National Public Health Institute, Finland
Sci Total Environ 382:130-4. 2007..5). These results suggest that interpretation of the meaning of short-term (<48 h) mold measurements in indoor and outdoor air samples must be made with caution... - Reduction in asthma morbidity in children as a result of home remediation aimed at moisture sourcesCarolyn M Kercsmar
Case Western Reserve University, Department of Pediatrics, Rainbow Babies and Children s Hospital, Cleveland, Ohio 44106, USA
Environ Health Perspect 114:1574-80. 2006..Home dampness and the presence of mold and allergens have been associated with asthma morbidity. We examined changes in asthma morbidity in children as a result of home remediation aimed at moisture sources...