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| G T AnkleySummaryAffiliation: U.S. Environmental Protection Agency Country: USA Publications
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Publications
Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessmentGerald T Ankley
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 29:730-41. 2010..In the concluding sections of the paper, we discuss how AOPs can help to guide research that supports chemical risk assessments and advocate for the incorporation of this approach into a broader systems biology framework...- The fathead minnow in aquatic toxicology: past, present and futureGerald T Ankley
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
Aquat Toxicol 78:91-102. 2006.... - Repeating history: pharmaceuticals in the environmentGerald T Ankley
U.S. EPA, USA
Environ Sci Technol 41:8211-7. 2007 - Temporal evaluation of effects of a model 3?-hydroxysteroid dehydrogenase inhibitor on endocrine function in the fathead minnowGerald T Ankley
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Lab, Duluth, Minnesota, USA
Environ Toxicol Chem 30:2094-102. 2011..This information is important to the design and interpretation of approaches for assessing the occurrence and effects of HPG-active chemicals in both the laboratory and the field... - Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnowGerald T Ankley
U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 22:1350-60. 2003..Given the widespread use of trenbolone acetate as a growth promoter, and relative stability of its metabolites in animal wastes, further studies are warranted to assess potential ecological risk... - Reproductive and developmental toxicity and bioconcentration of perfluorooctanesulfonate in a partial life-cycle test with the fathead minnow (Pimephales promelas)Gerald T Ankley
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 24:2316-24. 2005.... - Effects of two fungicides with multiple modes of action on reproductive endocrine function in the fathead minnow (Pimephales promelas)Gerald T Ankley
U S Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Toxicol Sci 86:300-8. 2005..These studies contribute to a better mechanistic understanding of the extrapolation of effects of endocrine-disrupting chemicals across vertebrate classes... - Assessment of environmental stressors potentially responsible for malformations in North American anuran amphibiansGerald T Ankley
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804 2595, USA
Ecotoxicol Environ Saf 58:7-16. 2004.... - Relationship of plasma sex steroid concentrations in female fathead minnows to reproductive success and population statusGerald T Ankley
US Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 USA
Aquat Toxicol 88:69-74. 2008..Results of our analysis illustrate a conceptual framework whereby a commonly measured biomarker, sex steroid status, could be linked to individual- and population-level effects in fish... - Evaluation of the model anti-androgen flutamide for assessing the mechanistic basis of responses to an androgen in the fathead minnow (Pimephales promelas)Gerald T Ankley
US Environmental Protection Agency, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Sci Technol 38:6322-7. 2004..The results of these studies provide data demonstrating the validity of using the fathead minnow as a model species for detecting EDCs that exert toxicity through interactions with the AR... - Endocrine disrupting chemicals in fish: developing exposure indicators and predictive models of effects based on mechanism of actionGerald T Ankley
USEPA, National Health and Environmental Effects Research Lab, Duluth, MN, USA
Aquat Toxicol 92:168-78. 2009..Although our project is focused specifically on EDCs in fish, we believe that the basic conceptual approach has utility for systematically assessing exposure and effects of chemicals with other MOA across a variety of biological systems... - Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish modelGerald T Ankley
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
Aquat Toxicol 99:389-96. 2010.... - Partial life-cycle toxicity and bioconcentration modeling of perfluorooctanesulfonate in the northern leopard frog (Rana pipiens)Gerald T Ankley
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 23:2745-55. 2004..Overall, our studies suggest that R. pipiens is not exceptionally sensitive to PFOS in terms of either direct toxicity or bioconcentration potential of the chemical... - Toxicogenomics in regulatory ecotoxicologyGerald T Ankley
U.S. EPA
Environ Sci Technol 40:4055-65. 2006 - Evaluation of the aromatase inhibitor fadrozole in a short-term reproduction assay with the fathead minnow (Pimephales promelas)Gerald T Ankley
U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Toxicol Sci 67:121-30. 2002.... - Reactivity profiles of ligands of mammalian retinoic acid receptors: a preliminary COREPA analysisG T Ankley
US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
SAR QSAR Environ Res 13:365-77. 2002..Variations in reactivity profiles for the RAR versus RXR families would be consistent with established differences in their affinity for endogenous retinoids, likely reflecting functional differences in the receptors... - Assessment of the risk of solar ultraviolet radiation to amphibians. I. Dose-dependent induction of hindlimb malformations in the northern leopard frog (Rana pipiens)Gerald T Ankley
U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Sci Technol 36:2853-8. 2002..The results of this study, in conjunction with measured or predicted exposure data from natural settings, provide a basis for quantitative prediction of the risk of solar UV radiation to amphibians... - Dynamic nature of alterations in the endocrine system of fathead minnows exposed to the fungicide prochlorazGerald T Ankley
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Duluth, Minnesota 55804, USA
Toxicol Sci 112:344-53. 2009..These studies demonstrate the adaptability of the HPG axis to chemical stress and highlight the need to consider the dynamic nature of the system when developing approaches to assess potential risks of endocrine-active chemicals... - Ketoconazole in the fathead minnow (Pimephales promelas): reproductive toxicity and biological compensationGerald T Ankley
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 26:1214-23. 2007..This has important implications for the interpretation of data from tests with endocrine-active chemicals... - Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas)K M Jensen
U S Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
Comp Biochem Physiol C Toxicol Pharmacol 128:127-41. 2001..Concentrations of beta-estradiol in females and 11-ketotestosterone in males were positively correlated with testosterone concentrations... - Stage- and species-specific developmental toxicity of all-trans retinoic acid in four native North American ranids and Xenopus laevisS J Degitz
U S Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Toxicol Sci 57:264-74. 2000..The significance of these findings is discussed in the context of the possible occurrence of retinoid mimics in the environment... - Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas)G T Ankley
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 20:1276-90. 2001..These studies demonstrate the utility of this short-term assay for identifying chemicals that exert reproductive toxicity through alterations in endocrine systems controlled by estrogens and androgens... - Masculinization of female mosquitofish in Kraft mill effluent-contaminated Fenholloway River water is associated with androgen receptor agonist activityL G Parks
Endocrinology Branch, National Health and Environmental Effects Research Laboratory (NHEERL, USEPA, RTD, Research Triangle Park, NC 27711, USA
Toxicol Sci 62:257-67. 2001..In summary, water collected downstream of the Kraft mill on the Fenholloway River contains unidentified androgenic substances whose presence is associated with masculinization of female mosquitofish... - Ambient solar UV radiation causes mortality in larvae of three species of Rana under controlled exposure conditionsJ E Tietge
Mid Continent Ecology Division, U S Environmental Protection Agency, 6201 Congdon Blvd, Duluth, MN 55804, USA
Photochem Photobiol 74:261-8. 2001.... - Comparing the effects of stage and duration of retinoic Acid exposure on amphibian limb development: chronic exposure results in mortality, not limb malformationsSigmund J Degitz
U.S. Environmental Protection Agency, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Toxicol Sci 74:139-46. 2003..From these experiments and our previous work, we conclude that it is unlikely that retinoid mimics would produce the spectrum of limb malformations which recently have been observed in amphibians collected from the field... - Small fish models for identifying and assessing the effects of endocrine-disrupting chemicalsGerald T Ankley
Mid-Continent Ecology Division, US Environmental Protection Agency Office of Research and Development, National Health and Environmental Effects Research Laboratory, Duluth, MN, USA
ILAR J 45:469-83. 2004..Examples of results with specific chemicals in tests with the fathead minnow, medaka, and zebrafish are presented and discussed in terms of sensitivity and specificity for different classes of EDCs... - Inhibition of retinoid activity by components of a paper mill effluentPatrick K Schoff
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Duluth, Minnesota 55811, USA
Environ Pollut 119:1-4. 2002..In a limited fractionation through a C-18 solid phase-exchange column the inhibitory activity was retained in the aqueous fraction, indicating that the activity was polar... - In utero exposure to the environmental androgen trenbolone masculinizes female Sprague-Dawley ratsA K Hotchkiss
Reproductive Toxicology Division, Endocrinology Branch, MD 72, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Toxicol Lett 174:31-41. 2007..5mg/day, 1.0mg/day, or 2.0mg/day groups. These changes were associated with amniotic fluid concentrations of TB that compare favorably with concentrations known to be active in both in vitro systems and in fish... - Investigating compensation and recovery of fathead minnow (Pimephales promelas) exposed to 17alpha-ethynylestradiol with metabolite profilingD R Ekman
Ecosystems Research Division, U S EPA, 960 College Station Road, Athens, Georgia 30605, USA
Environ Sci Technol 42:4188-94. 2008..This revealed an apparent ability of the fish to compensate for the presence of EE2 later in the exposure, and to partially recover from its effects after the chemical was removed... - NMR analysis of male fathead minnow urinary metabolites: a potential approach for studying impacts of chemical exposuresD R Ekman
Ecosystems Research Division, U S EPA, 960 College Station Road, Athens, GA 30605, United States
Aquat Toxicol 85:104-12. 2007..The results of these investigations clearly demonstrate the potential utility of an NMR-based approach for assessing chemical exposures in male fathead minnow, using urine collected from individual fish... - Hypoxia alters gene expression in the gonads of zebrafish (Danio rerio)Dalma Martinovic
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN, USA, 55804, USA
Aquat Toxicol 95:258-72. 2009..g., hydroxysteroid dehydrogenase alterations, downregulation of contractile elements, etc.)... - Impacts of an anti-androgen and an androgen/anti-androgen mixture on the metabolite profile of male fathead minnow urineTimothy W Collette
Office of Research and Development, National Exposure Research Laboratory, U S Environmental Protection Agency, 960 College Station Rd, Athens, Georgia 30605, USA
Environ Sci Technol 44:6881-6. 2010..The relative occurrence of male-like nuptial tubercles in female FHM exposed to TB and to the mixtures of TB and CA supported the metabolomics data... - Altered gene expression in the brain and ovaries of zebrafish (Danio rerio) exposed to the aromatase inhibitor fadrozole: microarray analysis and hypothesis generationL Villeneuve
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesosta 55804, USA
Environ Toxicol Chem 28:1767-82. 2009..These hypotheses and others derived from the microarray results provide a foundation for future studies aimed at understanding responses of the HPG axis to EACs and other chemical stressors... - Characterization of the androgen-sensitive MDA-kb2 cell line for assessing complex environmental mixturesLindsey S Blake
Integrated Biosciences, University of Minnesota, Duluth, 1035 University Dr, Duluth, Minnesota 55812, USA
Environ Toxicol Chem 29:1367-76. 2010.... - Transcription of key genes regulating gonadal steroidogenesis in control and ketoconazole- or vinclozolin-exposed fathead minnowsDaniel L Villeneuve
U S Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Toxicol Sci 98:395-407. 2007.... - Reproductive toxicity of vinclozolin in the fathead minnow: confirming an anti-androgenic mode of actionDalma Martinovic
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US EPA, Duluth, MN 55804, USA
Environ Toxicol Chem 27:478-88. 2008.... - Comparison of fathead minnow ovary explant and H295R cell-based steroidogenesis assays for identifying endocrine-active chemicalsDaniel L Villeneuve
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN, 55804, USA
Ecotoxicol Environ Saf 68:20-32. 2007..Overall, however, results of this study suggest that both the H295R and fathead minnow ovary explant assays have utility for identifying endocrine-active chemicals in screening-type applications... - Mechanistic computational model of ovarian steroidogenesis to predict biochemical responses to endocrine active compoundsMichael S Breen
National Center for Computational Toxicology, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Ann Biomed Eng 35:970-81. 2007..This capability could be useful for pharmaceutical development and environmental health assessments with EAC... - Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissueDaniel L Villeneuve
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804, USA
Aquat Toxicol 98:354-66. 2010.... - DNA microarray application in ecotoxicology: experimental design, microarray scanning, and factors affecting transcriptional profiles in a small fish speciesRong Lin Wang
Ecological Exposure Research Division, National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Environ Toxicol Chem 27:652-63. 2008..These findings will help to improve applications of microarrays in future ecotoxicological studies... - DNA microarray-based ecotoxicological biomarker discovery in a small fish model speciesRong Lin Wang
Ecological Exposure Research Division, National Exposure Research Lab, US Environmental Protection Agency, Cincinnati, Ohio 45268, USA
Environ Toxicol Chem 27:664-75. 2008..These gene classifiers could serve as potential biomarkers of exposure to specific classes of endocrine disruptors... - A transcriptomics-based biological framework for studying mechanisms of endocrine disruption in small fish speciesRong Lin Wang
USEPA, Ecological Exposure Research Division, National Exposure Research Laboratory, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA
Aquat Toxicol 98:230-44. 2010..These candidate networks/pathways have a variety of profound impacts on such cellular functions as stress response, cell cycle, and apoptosis... - II: Effects of a dopamine receptor antagonist on fathead minnow dominance behavior and ovarian gene expression in the fathead minnow and zebrafishDaniel L Villeneuve
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804, USA
Ecotoxicol Environ Saf 73:478-85. 2010..Results suggest that non-lethal concentrations of haloperidol do not induce ovarian molecular responses that could serve as biomarkers of exposure to D2R antagonists, but may impact behavior... - I. Effects of a dopamine receptor antagonist on fathead minnow, Pimephales promelas, reproductionDaniel L Villeneuve
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
Ecotoxicol Environ Saf 73:472-7. 2010..Results suggest that non-lethal concentrations of haloperidol do not directly impair fish reproduction. Potential effects of haloperidol on reproductive behaviors and gene expression were examined in a companion study... - Assessment of the risk of solar ultraviolet radiation to amphibians. III. Prediction of impacts in selected northern midwestern wetlandsStephen A Diamond
U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Sci Technol 36:2866-74. 2002..However, continued reduction of ozone and other global climate change effects may increase UV doses in wetlands, suggesting that the risk of UV to amphibians should continue to be monitored and studied... - Effects of a 3beta-hydroxysteroid dehydrogenase inhibitor, trilostane, on the fathead minnow reproductive axisDaniel L Villeneuve
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Office of Research and Development, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Toxicol Sci 104:113-23. 2008.... - Direct effects, compensation, and recovery in female fathead minnows exposed to a model aromatase inhibitorDaniel L Villeneuve
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Health Perspect 117:624-31. 2009..Several chemicals in the environment have the potential to inhibit aromatase, an enzyme critical to estrogen synthesis... - Temporal variation in the estrogenicity of a sewage treatment plant effluent and its biological significanceDalma Martinovic
Department of Fisheries, Wildlife, and Conservation Biology, 1980 Folwell Avenue, University of Minnesota, St Paul, Minnesota 55108, USA
Environ Sci Technol 42:3421-7. 2008..The variability of the effluent's estrogenicity suggests that studies concerned with the effects of STP effluents should collect multiple daily samples and then test them on an appropriate time-weighted basis... - Linkage of biochemical responses to population-level effects: a case study with vitellogenin in the fathead minnow (Pimephales promelas)David H Miller
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Grosse Ile, Michigan 48138, USA
Environ Toxicol Chem 26:521-7. 2007..2% of the preexposed population. Overall, the current study provides an example of how changes in a biomarker (VTG concentration) can be quantitatively translated into adverse effects at the individual and population levels... - Ecotoxicogenomics: linkages between exposure and effects in assessing risks of aquatic contaminants to fishAnn L Miracle
U S Environmental Protection Agency, Ecological Exposure Research Division, Cincinnati, OH, USA
Reprod Toxicol 19:321-6. 2005..The challenges of the individual technologies and the combinations of tools for ecotoxicogenomics are discussed in application to aquatic toxicology with a particular emphasis on fish testing... - Comparison of relative binding affinities of endocrine active compounds to fathead minnow and rainbow trout estrogen receptorsJeffrey S Denny
U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 24:2948-53. 2005..Comparison of RBAs of the chemicals tested for fhmER and rbtER indicates that the rank order of RBAs essentially are the same for both species... - Relationship between brain and ovary aromatase activity and isoform-specific aromatase mRNA expression in the fathead minnow (Pimephales promelas)Daniel L Villeneuve
US Environmental Protection Agency, Mid Continent Ecology Division, Duluth, MN, USA
Aquat Toxicol 76:353-68. 2006.... - Induction of an estrogen-responsive reporter gene in rainbow trout hepatoma cells (RTH 149) at 11 or 18 degrees CMichael W Hornung
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 22:866-71. 2003.... - Uptake and metabolism of all-trans retinoic acid by three native north American ranidsPatricia A Kosian
US EPA, National Health and Environmental Effects Research Lab, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
Toxicol Sci 74:147-56. 2003.... - Developmental toxicity of methoprene and several degradation products in Xenopus laevisSigmund J Degitz
US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 2595, USA
Aquat Toxicol 64:97-105. 2003..01 mg/l, suggests that concerns for methoprene-mediated developmental toxicity to amphibians may be unwarranted... - Overview of data and conceptual approaches for derivation of quantitative structure-activity relationships for ecotoxicological effects of organic chemicalsSteven P Bradbury
U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 22:1789-98. 2003..Finally, an overview of expert systems that integrate chemical-specific mode of action classification and associated QSAR selection for estimating potential toxicological effects of organic chemicals is presented... - Mechanistic basis for estrogenic effects in fathead minnow (Pimephales promelas) following exposure to the androgen 17alpha-methyltestosterone: conversion of 17alpha-methyltestosterone to 17alpha-methylestradiolMichael W Hornung
United States Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
Aquat Toxicol 66:15-23. 2004..3% of E2, support the hypothesis that aromatization of MT to ME2 contributes to the estrogenic effects in fathead minnows following exposure to this androgen... - Cloning and in vitro expression and characterization of the androgen receptor and isolation of estrogen receptor alpha from the fathead Minnow (Pimephales promelas)Vickie S Wilson
US Environmental Protection Agency, ORD, NHEERL, Reproductive Toxicology Division, North Carolina 27711, USA
Environ Sci Technol 38:6314-21. 2004..Future plans include comparison of binding affinities of the fhAR to those of the human AR, also expressed in COS cells, using a range of EDCs... - Characterization of responses to the antiandrogen flutamide in a short-term reproduction assay with the fathead minnowKathleen M Jensen
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 USA
Aquat Toxicol 70:99-110. 2004..Consequently, our findings suggest that the 21-day reproduction assay utilizing fathead minnows is a sensitive short-term screening method for the detection of endocrine-disrupting chemicals, including antiandrogens... - Adverse effects of environmental antiandrogens and androgens on reproductive development in mammalsL Earl Gray
MD 72, Endocrinology Branch, Reproductive Toxicology Division, NHEERL, ORD, US Environmental Protection Agency, RTP, NC 27771, USA
Int J Androl 29:96-104; discussion 105-8. 2006.... - Evaluation of a commercial kit for measuring vitellogenin in the fathead minnow (Pimephales promelas)Kathleen M Jensen
National Health and Environmental Effects Research Laboratory, Office of Research and Development, Mid Continent Ecology Division, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA
Ecotoxicol Environ Saf 64:101-5. 2006..Results obtained using the two different ELISA methods were consistently similar... - A graphical systems model to facilitate hypothesis-driven ecotoxicogenomics research on the teleost brain-pituitary-gonadal axisDaniel L Villeneuve
Mid Continent Ecology Division, U S EPA, USA
Environ Sci Technol 41:321-30. 2007..Furthermore, microarray results helped identify additional elements (genes/ proteins) that could be included in the model to potentially increase its predictive capacity. With proper recognition of.. - Development of quantitative real-time PCR assays for fathead minnow (Pimephales promelas) gonadotropin beta subunit mRNAs to support endocrine disruptor researchDaniel L Villeneuve
United States Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55803, USA
Comp Biochem Physiol C Toxicol Pharmacol 145:171-83. 2007..Results of such experiments should facilitate a greater understanding of integrated system-wide responses of the fathead minnow brain-pituitary-gonadal axis to stressors including EDCs... - Evaluation of the methoxytriazine herbicide prometon using a short-term fathead minnow reproduction test and a suite of in vitro bioassaysDaniel L Villeneuve
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Divison, 6201 Congdon Boulevard, Duluth, Minnesota, USA
Environ Toxicol Chem 25:2143-53. 2006..The relevance of these effects to hazard assessment for the pesticide is uncertain... - Modeling impacts on populations: fathead minnow (Pimephales promelas) exposure to the endocrine disruptor 17beta-trenbolone as a case studyDavid H Miller
Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 9311 Groh Rd, Grosse Ile, MI 48138, USA
Ecotoxicol Environ Saf 59:1-9. 2004..Overall, fathead minnow populations exposed to continued concentrations of 17beta-trenbolone equal to or greater than 0.027 microg/L were projected to have average equilibrium population sizes that approached zero... - Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlotElizabeth J Durhan
Mid Continent Ecology Division, National Health and Environmental Effects Laboratory, U S Environmental Protection Agency, Duluth, Minnesota 55804, USA
Environ Health Perspect 114:65-8. 2006..Further work is needed to ascertain the degree to which synthetic androgens such as trenbolone contribute to androgenic activity of feedlot discharges... - Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluentEth J Durhan
US Environmental Protection Agency, National Health and Environmental Effects Laboratory, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 21:1973-6. 2002..We conclude that, although androstenedione was present at easily detectable concentrations in the river water (> 100 ng/L), this compound is not associated with androgenic activity of water from the site... - Effects of the feedlot contaminant 17alpha-trenbolone on reproductive endocrinology of the fathead minnowKathleen M Jensen
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Sci Technol 40:3112-7. 2006..The present study demonstrates the importance of considering both alpha- and beta-trenbolone in assessing the potential ecological risk of androgens associated with beef feedlot discharges... - Use of gene expression, biochemical and metabolite profiles to enhance exposure and effects assessment of the model androgen 17?-trenbolone in fishDrew R Ekman
U S Environmental Protection Agency, Athens, Georgia, USA
Environ Toxicol Chem 30:319-29. 2011..Furthermore, TRB appeared to induce the hepatic metabolome of females to become more like that of males at both high test concentrations of TRB (472 ng/L) and more environmentally relevant levels (33 ng/L)... - Evaluation of bioaccumulation and photo-induced toxicity of fluoranthene in larval and adult life-stages of Chironomus tentansH E Bell
Office of Science and Technology, Office of Water, US Environmental Protection Agency, MC4304T, Washington DC 20460, USA
Arch Environ Contam Toxicol 47:297-303. 2004..These studies indicated that both the burrowing behavior of the larvae and the elimination of fluoranthene in molted exuviae are protective mechanisms against photo-enhanced PAH toxicity in this species... - Effects of low dissolved oxygen on organisms used in freshwater sediment toxicity testsVincent R Mattson
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
Chemosphere 70:1840-4. 2008..12 mg O(2)l(-1). The combination of these data with other literature data suggest that DO minima in current North American 10-d sediment test guidelines are reasonable... - Effects of methoprene, its metabolites, and breakdown products on retinoid-activated pathways in transfected cell linesPatrick K Schoff
U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 23:1305-10. 2004.... - Xenoendocrine disrupters-tiered screening and testing: filling key data gapsL E Gray
Endocrinology Branch, Reproductive Toxicology Division, National Health Environmental Effects Research Laboratory, ORD, US Environmental Protection Agency, MD 72, Research Triangle Park, NC 27111, USA
Toxicology 181:371-82. 2002..Tier 1 and 2 also should examine relevant mixtures of EDCs. Toxicants that induce malformations in AR-dependent tissues produce cumulative effects even when two chemicals act via different mechanisms of action... - Amendment of sediments with a carbonaceous resin reduces bioavailability of polycyclic aromatic hydrocarbonsC W West
U S Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
Environ Toxicol Chem 20:1104-11. 2001.... - Computational toxicology: framework, partnerships, and program development. September 29-30, 2003, Research Triangle Park, North CarolinaRobert Kavlock
Office of Research and Development, US Environmental Protection Agency, Washington, DC, USA
Reprod Toxicol 19:265-80. 2005 - Evaluation of time-to-effects as a basis for quantifying the toxicity of contaminated sedimentsDavid L Defoe
Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA
Chemosphere 51:1-5. 2003..These studies demonstrate the feasibility of use of time-to-effects as a basis for quantifying the relative toxicity of contaminated sediments... - Ecotoxicology--a multidisciplinary, problem-driven scienceJoop L M Hermens
Urecht University, The Netherlands
Environ Sci Technol 38:446A-447A. 2004 - Screening and testing for endocrine disruption in fish-biomarkers as "signposts," not "traffic lights," in risk assessmentThomas H Hutchinson
AstraZeneca Global Safety, Health and Environment, Brixham Environmental Laboratory, United Kingdom
Environ Health Perspect 114:106-14. 2006.... - What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelasKaren H Watanabe
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 N W Walker Rd, Beaverton, OR 97006, USA
Comp Biochem Physiol C Toxicol Pharmacol 146:348-56. 2007..Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow... - Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas)Patrick Larkin
EcoArray, 12085 Research Drive, Alachua, Florida 32615, USA
Environ Toxicol Chem 26:1497-506. 2007..Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species... - Comparison of ELISAs for detecting vitellogenin in the fathead minnow (Pimephales promelas)Eve Mylchreest
DuPont Haskell Laboratory for Health and Environmental Sciences, Elkton Road, PO Box 50, Newark, DE 19714, USA
Comp Biochem Physiol C Toxicol Pharmacol 134:251-7. 2003..The homologous fVTG assay is recommended to achieve accurate quantification of VTG levels in fathead minnows... - Fifteen years after "Wingspread"--environmental endocrine disrupters and human and wildlife health: where we are today and where we need to goAndrew K Hotchkiss
USEPA NCSU Cooperative Training agreement CT826512010, Raleigh, North Carolina 27695, USA
Toxicol Sci 105:235-59. 2008.... - Application of frog embryo teratogenesis assay-Xenopus to ecological risk assessmentRobert A Hoke
DuPont, Haskell Laboratory for Health and Environmental Sciences, Newark, Delaware 19714, USA
Environ Toxicol Chem 24:2677-90. 2005.... - Perturbation of gene expression and steroidogenesis with in vitro exposure of fathead minnow ovaries to ketoconazoleEdward J Perkins
Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, MS 39180, USA
Mar Environ Res 66:113-5. 2008.... - Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibitionChad R Blystone
Department of Environmental and Molecular Toxicology, NC State University, Raleigh, NC 27695, USA
Toxicol Sci 97:512-9. 2007..These results demonstrate that PCZ lowers testicular testosterone synthesis by inhibiting CYP17 activity which likely contributes to the induced malformations in androgen-dependent tissues of male offspring...