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Genomes and Genes | Janos ZempleniSummaryAffiliation: University of Nebraska Country: USA Publications
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The role of holocarboxylase synthetase in genome stability is mediated partly by epigenomic synergies between methylation and biotinylation eventsJanos Zempleni
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
Epigenetics 6:892-4. 2011..It appears that histone biotinylation depends on prior methylation of cytosines. De-repression of long terminal repeats in biotin- or HLCS-deficient cell cultures and organisms is associated with genome instability...- Uptake, localization, and noncarboxylase roles of biotinJanos Zempleni
Department of Nutrition and Health Sciences and Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Nebraska 68583 0806, USA
Annu Rev Nutr 25:175-96. 2005..Roles for biotin in cell signaling and chromatin structure are consistent with the notion that biotin has a unique significance in cell biology... - Repression of transposable elements by histone biotinylationJanos Zempleni
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
J Nutr 139:2389-92. 2009..Further, we hypothesize that this biotinylation promotes the subsequent dimethylation of lysine-9 in histone H3, resulting in an overall synergistic effect of 3 diet-dependent covalent modifications of histones in the repression of LTR... - BiotinJanos Zempleni
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
Biofactors 35:36-46. 2009..Possible effects of inadequate biotin intake, drug interactions, and inborn errors of metabolism are discussed, including putative effects on birth defects... - Sodium-dependent multivitamin transporter gene is regulated at the chromatin level by histone biotinylation in human Jurkat lymphoblastoma cellsJanos Zempleni
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
J Nutr 139:163-6. 2009.... - Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogasterYap Ching Chew
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
J Nutr 138:2316-22. 2008..Collectively, this study reveals a novel diet-dependent epigenetic mechanism that could affect cancer risk... - Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cellsSarah E R H Crisp
Dept. of Nutritional Science and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA
Eur J Nutr 43:23-31. 2004..It is uncertain whether activities of biotin-dependent pathways in placenta affect fetal development in vivo... - HepG2 cells develop signs of riboflavin deficiency within 4 days of culture in riboflavin-deficient mediumRicarda Werner
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA
J Nutr Biochem 16:617-24. 2005..These studies are consistent with the hypothesis that HepG2 cells have a great demand for riboflavin and that cell stress develops rapidly if riboflavin supply is marginally low... - Biotinylation of K12 in histone H4 decreases in response to DNA double-strand breaks in human JAr choriocarcinoma cellsNagarama Kothapalli
Department of Biochemistry, University of Nebraska, Lincoln, NE, USA
J Nutr 135:2337-42. 2005..These studies suggest that the lack of biotinylation of K12 in histone H4 is an early signaling event in response to DSB... - Biotin deficiency stimulates survival pathways in human lymphoma cells exposed to antineoplastic drugsJacob B Griffin
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0806, USA
J Nutr Biochem 16:96-103. 2005..Collectively, these observations suggest that biotin deficiency may enhance resistance of cancer cells to antineoplastic agents... - Biotinylation is a natural, albeit rare, modification of human histonesToshinobu Kuroishi
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
Mol Genet Metab 104:537-45. 2011..In this model, docking of HCS in chromatin causes the occasional binding of biotin to histones as a tracer for HCS binding sites... - Biotin supplementation decreases the expression of the SERCA3 gene (ATP2A3) in Jurkat cells, thus, triggering unfolded protein responseJacob B Griffin
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583-0806, USA
J Nutr Biochem 17:272-81. 2006..Collectively, this study suggests that supplements containing pharmacological doses of biotin may cause cell stress by impairing protein folding in the ER... - Lysine residues in N-terminal and C-terminal regions of human histone H2A are targets for biotinylation by biotinidaseYap Ching Chew
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 68583-0806, USA
J Nutr Biochem 17:225-33. 2006..Collectively, these studies have identified five novel biotinylation sites in human histones; histone H2A is unique among histones in that its biotinylation sites include amino acid residues from the C-terminus... - Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patternsErin M Smith
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
J Nutr 137:2006-12. 2007..Biotinylation of histones was barely detectable in biotin-deprived flies, suggesting that epigenetic events might have contributed to effects of biotin deprivation... - K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cellsGabriela Camporeale
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 18:760-8. 2007..These data are consistent with a novel role for biotin in chromatin structure and transcriptional activity of genes... - Novel histone biotinylation marks are enriched in repeat regions and participate in repression of transcriptionally competent genesValerie Pestinger
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 22:328-33. 2011..Importantly, the enrichment of H3K9bio, H3K18bio and H4K8bio at genomic loci depended on the concentration of biotin in culture media at nutritionally relevant levels, suggesting a novel mechanism of gene regulation by biotin... - Biotin regulates the expression of holocarboxylase synthetase in the miR-539 pathway in HEK-293 cellsBaolong Bao
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA
J Nutr 140:1546-51. 2010..Collectively, the results of this study suggest that miR-539 is among the factors sensing biotin and regulating HCS... - Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3Keyna Kobza
Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
BMB Rep 41:310-5. 2008..Moreover the biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin... - K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblastsSubhashinee S K Wijeratne
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 21:310-6. 2010..Collectively, these data suggest that H4K12bio is enriched in telomeric repeats and represents a novel epigenetic mark for cell senescence... - K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidaseKeyna Kobza
Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0806, USA
FEBS J 272:4249-59. 2005..We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation... - Use of synthetic peptides for identifying biotinylation sites in human histonesGabriela Camporeale
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
Methods Mol Biol 418:139-48. 2008..Amino acid substitutions (e.g., lysine-to-alanine substitutions) in synthetic peptides can be used to corroborate identification of biotinylation sites... - High-throughput immunoblotting identifies biotin-dependent signaling proteins in HepG2 hepatocarcinoma cellsRocio Rodriguez-Melendez
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
J Nutr 135:1659-66. 2005.... - Susceptibility to heat stress and aberrant gene expression patterns in holocarboxylase synthetase-deficient Drosophila melanogaster are caused by decreased biotinylation of histones, not of carboxylasesGabriela Camporeale
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
J Nutr 137:885-9. 2007..We conclude that gene expression patterns and phenotypes in HCS-deficient flies in previous studies are caused by decreased biotinylation of histones rather than MCC... - Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18Baolong Bao
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
J Nutr Biochem 22:470-5. 2011..We speculate that the targeting of HCS to distinct regions in human chromatin is mediated by DNA sequence, biotin, RNA, epigenetic marks or chromatin proteins... - Riboflavin deficiency causes protein and DNA damage in HepG2 cells, triggering arrest in G1 phase of the cell cycleKaroline C Manthey
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583-0806, USA, USA
J Nutr Biochem 17:250-6. 2006..We conclude that riboflavin deficiency is associated with oxidative damage to proteins and DNA in liver cells, leading to cell stress and G1 phase arrest... - Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cellsGaganpreet Kaur Mall
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
J Nutr 140:1086-92. 2010..The mechanisms of biotin homeostasis are qualitatively similar but quantitatively different in HepG2 and Jurkat cells; HCS, histone biotinylation, and biotin transporters play a role in homeostasis in both... - Biotin supplementation increases expression of the cytochrome P450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaksRocio Rodriguez-Melendez
Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
J Nutr 134:2222-8. 2004..These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks... - Expression of oncogenes depends on biotin in human small cell lung cancer cells NCI-H69Sarah B Scheerger
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
Int J Vitam Nutr Res 73:461-7. 2003..The clinical significance of effects of biotin supply on expression of oncogenes remains to be elaborated... - Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cellsRachel L Daberkow
Departments of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
J Nutr 133:2703-6. 2003..These findings are consistent with the hypothesis that MCT mediate biotin uptake in human lymphoid cells... - Clusters of biotin-responsive genes in human peripheral blood mononuclear cellsSilke Wiedmann
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA
J Nutr Biochem 15:433-9. 2004..This suggests that position effects play a role in biotin-dependent gene expression. Collectively, these findings suggest that the human genome contains clusters of biotin-dependent genes... - Jurkat cells respond to biotin deficiency with increased nuclear translocation of NF-kappaB, mediating cell survivalRocio Rodriguez-Melendez
Department of Nutrition and Health Science, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA
Int J Vitam Nutr Res 74:209-16. 2004..These findings suggest that NF-kappaB mediates survival of biotin-deficient cells... - The expression of genes encoding ribosomal subunits and eukaryotic translation initiation factor 5A depends on biotin and bisnorbiotin in HepG2 cellsRocio Rodriguez Melendez
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583 0806, USA
J Nutr Biochem 17:23-30. 2006..Collectively, these data suggest that bisnorbiotin has biotin-like activities regarding gene expression, and that clusters of (bisnor)biotin-dependent genes include genes that play roles in translational activity... - Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activityBaolong Bao
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
Biochem Biophys Res Commun 412:115-20. 2011..We conclude this report by proposing a novel role for HLCS in epigenetic events, mediated by physical interactions between HLCS and other chromatin proteins as part of a larger multiprotein complex that mediates gene repression... - The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylationYousef I Hassan
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, NE 68583 0806, USA
Arch Biochem Biophys 495:35-41. 2010..We identified a Syn67 docking site near the active pocket of HCS by in silico modeling and site-directed mutagenesis. Biotinylation of proteins by HCS is more specific than previously assumed... - Interleukin-2 receptor-gamma -dependent endocytosis depends on biotin in Jurkat cellsRocio Rodriguez-Melendez
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA
Am J Physiol Cell Physiol 284:C415-21. 2003..Inhibition of endocytosis by genistein and overexpression of interleukin-2 receptor-gamma abolished the effect of biotin. These findings suggest that endocytosis of interleukin-2 depends on biotin... - Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cellsRocio Rodriguez-Melendez
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE, USA
J Nutr 133:1259-64. 2003..It is not known whether naturally occurring biotin metabolites also have biotin-like activities... - Biotinyl-methyl 4-(amidomethyl)benzoate is a competitive inhibitor of human biotinidaseKeyna A Kobza
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 19:826-32. 2008..Finally, biotinyl-methyl 4-(amidomethyl)benzoate did not affect biotin transport in human cells, suggesting specificity in regard to biotin-related processes... - Regulation of gene expression by biotin (review)Rocio Rodriguez-Melendez
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, NE 68583, USA
J Nutr Biochem 14:680-90. 2003..These new insights into biotin-dependent gene expression are likely to lead to a better understanding of roles for biotin in cell biology and fetal development... - An avidin-based assay for histone debiotinylase activity in human cell nucleiYap Ching Chew
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 18:475-81. 2007..This assay proved useful in monitoring activities of putative histone debiotinylases during their partial purification from cells. Collectively, this assay is a useful tool for investigating histone debiotinylases in human tissues... - Drosophila melanogaster holocarboxylase synthetase is a chromosomal protein required for normal histone biotinylation, gene transcription patterns, lifespan, and heat toleranceGabriela Camporeale
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
J Nutr 136:2735-42. 2006.... - Riboflavin deficiency impairs oxidative folding and secretion of apolipoprotein B-100 in HepG2 cells, triggering stress response systemsKaroline C Manthey
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583, USA
J Nutr 135:978-82. 2005..Decreased secretion of apoB in riboflavin-deficient cells might interfere with lipid homeostasis in vivo... - Biological functions of biotinylated histonesNagarama Kothapalli
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA
J Nutr Biochem 16:446-8. 2005..Evidence was provided that biotinylation of histones plays a role in cell proliferation, gene silencing and cellular response to DNA damage... - The nuclear abundance of transcription factors Sp1 and Sp3 depends on biotin in Jurkat cellsJacob B Griffin
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE, USA
J Nutr 133:3409-15. 2003..This study provides evidence that some effects of biotin on gene expression might be mediated by the nuclear abundance of Sp1 and Sp3... - Exposure to UV light causes increased biotinylation of histones in Jurkat cellsDorothea M Peters
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln 68583, USA
Am J Physiol Cell Physiol 283:C878-84. 2002..In summary, this study provided evidence that increased biotinylation of histones in DNA-damaged cells might either be a side product of carboxylase degradation or a step during apoptosis... - Oxidative folding of interleukin-2 is impaired in flavin-deficient jurkat cells, causing intracellular accumulation of interleukin-2 and increased expression of stress response genesGabriela Camporeale
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, USA
J Nutr 133:668-72. 2003..These findings are consistent with the hypothesis that flavin deficiency may cause cellular stress by accumulation of unfolded proteins in human cells... - Roles for nutrients in epigenetic eventsAnna M Oommen
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 16:74-7. 2005.... - K8 and K12 are biotinylated in human histone H4Gabriela Camporeale
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA
Eur J Biochem 271:2257-63. 2004.... - Biotinidase catalyzes debiotinylation of histonesTyrie D Ballard
Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 68583-0806, USA
Eur J Nutr 41:78-84. 2002..Rates of histone debiotinylation were significantly decreased in biotinidase-deficient samples. CONCLUSION: Debiotinylation of histones in human samples is an enzyme-mediated process that is at least partly catalyzed by biotinidase... - Holocarboxylase synthetase regulates expression of biotin transporters by chromatin remodeling events at the SMVT locusMichael Gralla
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
J Nutr Biochem 19:400-8. 2008.... - Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporterJacob B Griffin
Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA
Int J Vitam Nutr Res 72:195-8. 2002..Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT... - Nitric oxide signaling depends on biotin in Jurkat human lymphoma cellsRocio Rodriguez-Melendez
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
J Nutr 139:429-33. 2009..Collectively, the results of this study are consistent with the hypothesis that biotin-dependent cGMP signaling in human lymphoid cells is mediated by NO... - N- and C-terminal domains in human holocarboxylase synthetase participate in substrate recognitionYousef I Hassan
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
Mol Genet Metab 96:183-8. 2009..In silico predictions were consistent with observations from Y2H assays and yeast rescue experiments, and suggested docking of p67 near Arg508 and Ser515 within the central domain of HCS... - Biotin deficiency decreases life span and fertility but increases stress resistance in Drosophila melanogasterAnette Landenberger
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA
J Nutr Biochem 15:591-600. 2004..These findings are consistent with the hypothesis that biotin affects life span, stress resistance, and fertility in fruit flies... - A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetaseYousef I Hassan
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0806, USA
Nutr Rev 66:721-5. 2008..Decreased histone biotinylation decreases life span and stress resistance in Drosophila. Low enrichment of biotinylated histones at transposable elements impairs repression of these elements... - Genomic implications of H(2)O (2) for cell proliferation and growth of Caco-2 cellsTheresa A Herring
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583, USA
Dig Dis Sci 52:3005-15. 2007.... - Epigenetic regulation of chromatin structure and gene function by biotinYousef I Hassan
Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
J Nutr 136:1763-5. 2006..We propose that some effects of biotin deficiency can be attributed to abnormal chromatin structures... - Biotin supplementation increases expression of genes encoding interferon-gamma, interleukin-1beta, and 3-methylcrotonyl-CoA carboxylase, and decreases expression of the gene encoding interleukin-4 in human peripheral blood mononuclear cellsSilke Wiedmann
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, 68583, USA
J Nutr 133:716-9. 2003..These data suggest that biotin supplementation affects gene expression in human immune cells. Effects of biotin on gene expression are likely to modulate the response of immune cells to antigens... - Biotin supply affects expression of biotin transporters, biotinylation of carboxylases and metabolism of interleukin-2 in Jurkat cellsKaroline C Manthey
Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583-0806, USA
J Nutr 132:887-92. 2002..The physiological significance of effects of biotin status on metabolism of interleukin-2 remains to be elaborated... - A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activityLuisa Rios-Avila
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, NE 68583 0806, USA
Clin Chim Acta 412:735-9. 2011..HCS null mammals are not viable whereas HCS deficiency is linked to developmental delays in humans and phenotypes such as short life span and low stress resistance in Drosophila... - Biotin requirements for DNA damage preventionJanos Zempleni
Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
Mutat Res 733:58-60. 2012..These observations might offer a mechanistic explanation for some of the birth defects seen in biotin-deficient animal models... - Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technologyDipika Singh
Department of Biological Systems Engineering, University of Nebraska Lincoln, Lincoln, NE 68583, USA
Anal Biochem 413:55-9. 2011..We conclude that DamID is a useful technology to map HCS binding sites in human chromatin and propose that the entire set of HCS binding sites could be mapped by combining DamID with microarray technology... - Development of an internet based system for modeling biotin metabolism using Bayesian networksJinglei Zhou
Department of Statistics, University of Nebraska Lincoln, Lincoln, NE 68583, USA
Comput Methods Programs Biomed 104:254-9. 2011..This program enables convenient in silico experiments regarding biotin metabolism, which can help researchers design future experiments while new data can be continuously incorporated... - Biotin availability regulates expression of the sodium-dependent multivitamin transporter and the rate of biotin uptake in HepG2 cellsDiana Pacheco-Alvarez
, , , Mexico D.F. 04510, Mexico
Mol Genet Metab 85:301-7. 2005..Our results support the proposed role of SMVT in the altruistic regulation of biotin utilization in liver cells that has been associated with sparing biotin depletion of the brain... - Biotin dependency due to a defect in biotin transportRebecca Mardach
Regional Metabolic Service, Kaiser Permanente, Los Angeles, California, USA
J Clin Invest 109:1617-23. 2002..These results provide evidence for a novel genetic defect in biotin transport. This child is the first known with this defect, which should now be included in the identified causes of biotin dependency... - Biotin uptake into human peripheral blood mononuclear cells increases early in the cell cycle, increasing carboxylase activitiesJ Steven Stanley
Arkansas Children's Hospital Research Institute, Little Rock, AR, USA
J Nutr 132:1854-9. 2002..In summary, these findings suggest that PBMC respond to proliferation with increased biotin uptake early in the cell cycle, and that biotin is utilized to increase activities of two of the four biotin-requiring carboxylases...
Research Grants
- Biotin Deficiency Impairs Silencing of Repeat Regions & RetrotransposonsJanos Zempleni; Fiscal Year: 2010..This proposal lays the groundwork for decreasing the incidence of retrotransposon-associated diseases in groups at risk for developing biotin deficiency such as pregnant women and their fetuses. ..
- Biotin sensing and chromatin remodeling by holocarboxylase synthetaseJanos Zempleni; Fiscal Year: 2010..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..
- Biotin sensing and chromatin remodeling by holocarboxylase synthetaseJanos Zempleni; Fiscal Year: 2009..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..
- Epigenetic effects of biotin on activation of endogenous viral sequencesJanos Zempleni; Fiscal Year: 2007..These studies are likely to identify means by which manipulation of the environmental factor "diet" increases genomic stability and decreases cancer risk. ..
- Vitamin-dependent modifications of histonesJanos Zempleni; Fiscal Year: 2007..The potential role of biotin in these processes establishes a link between cellular biotin homeostasis and DNA mutation and abnormal proliferation such as in cancer cells. ..
- Essential role of biotin in cell proliferationJanos Zempleni; Fiscal Year: 2004..The basic knowledge generated may well be relevant to teratogenesis and immune dysfunction caused by biotin deficiency. ..
- Biotin sensing and chromatin remodeling by holocarboxylase synthetaseJanos Zempleni; Fiscal Year: 2010..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..