Research Topics
| Ryan TewheySummaryAffiliation: University of California Country: USA Publications
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Detail Information
Publications
Enrichment of sequencing targets from the human genome by solution hybridizationRyan Tewhey
Scripps Genomic Medicine, Scripps Translational Science Institute, The Scripps Research Institute, 3344 N Torrey Pines Court, La Jolla, CA 92037, USA
Genome Biol 10:R116. 2009..9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage of targets. We obtained 93% sensitivity to detect SNPs, with a calling accuracy greater than 99%...
Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolatesBernice Meng Qi Sim
Genome Institute of Singapore, 60 Biopolis Street, 138672 Singapore
Genome Biol 11:R89. 2010..To study B. thailandensis genomic variation, we profiled 50 isolates using a pan-genome microarray comprising genomic elements from 28 Burkholderia strains and species...
Efficient and cost effective population resequencing by pooling and in-solution hybridizationVikas Bansal
Scripps Genomic Medicine, Scripps Translational Science Institute, La Jolla, California, United States of America
PLoS ONE 6:e18353. 2011..6% of variants below 5% in frequency. Finally, allele frequencies for single nucleotide variants (SNVs) estimated from the pooled data and the HapMap genotype data were tightly correlated (correlation coefficient >? =? 0.995)...
Accurate detection and genotyping of SNPs utilizing population sequencing dataVikas Bansal
Scripps Translational Science Institute, The Scripps Research Institute, La Jolla, CA 92037, USA
Genome Res 20:537-45. 2010..Collectively, these results suggest that analysis of population sequencing data is a powerful approach for the accurate detection of SNPs and the assignment of genotypes to individual samples...
The importance of phase information for human genomicsRyan Tewhey
The Scripps Translational Science Institute, 3344 North Torrey Pines Road, Suite 300, La Jolla, California 92037, USA
Nat Rev Genet 12:215-23. 2011..Thus, the existing technological impediments to obtaining phase information must be overcome if human genomics is to reach its full potential...
Microdroplet-based PCR enrichment for large-scale targeted sequencingRyan Tewhey
Scripps Genomic Medicine, Scripps Translational Science Institute, The Scripps Research Institute, La Jolla, California, USA
Nat Biotechnol 27:1025-31. 2009..Our results demonstrate that microdroplet technology is well suited for processing DNA for massively parallel enrichment of specific subsets of the human genome for targeted sequencing...
