Research Topics
Species | R S StephensSummaryAffiliation: University of California Country: USA Publications
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Publications
Interaction of Chlamydia trachomatis with mammalian cells is independent of host cell surface heparan sulfate glycosaminoglycansRichard S Stephens
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
Infect Immun 74:1795-9. 2006..These data do not support the hypothesis and demonstrate that host cell surface heparan sulfate does not serve an essential functional role in chlamydial infectivity...- The cellular paradigm of chlamydial pathogenesisRichard S Stephens
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley 94720, USA
Trends Microbiol 11:44-51. 2003.... - Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatisR S Stephens
Program in Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
Infect Immun 68:1080-5. 2000..These findings suggest that heparin-coated beads and Chlamydia trachomatis enter eukaryotic cells by similar pathways... - Activation of the host cell proinflammatory interleukin-8 response by Chlamydia trachomatisKerry R Buchholz
Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
Cell Microbiol 8:1768-79. 2006..trachomatis. We conclude that a C. trachomatis growth-dependent factor produced at mid-developmental stage induces IL-8 within the epithelial cell it infects through activation of host signalling pathways... - Chlamydia attachment to mammalian cells requires protein disulfide isomeraseCarolyn G Conant
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
Cell Microbiol 9:222-32. 2007..The cell line was also resistant to diphtheria toxin and required complemented cell-surface PDI for toxin entry. These data demonstrate that native PDI at the cell surface is required for effective chlamydial attachment and infectivity... - Heparin-binding outer membrane protein of chlamydiaeR S Stephens
Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
Mol Microbiol 40:691-9. 2001..Surface localization of OmcB was shown using monospecific antisera specific to the 20-mer OmcB peptide that bound the surfaces of elementary bodies (EB) and by heparin-binding peptide cross-linking of EB surface proteins... - Chlamydial genomics and vaccine antigen discoveryR S Stephens
Program in Infectious Diseases, University of California, Berkeley, CA 94720 7360
J Infect Dis 181:S521-3. 2000..This knowledge will aid vaccine antigen discovery by facilitating the identification, testing, and evaluation of antigens by way of vaccine delivery approaches that elicit protective immunity... - Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatisR S Stephens
Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA
Science 282:754-9. 1998..The phylogenetic mosaic of chlamydial genes, including a large number of genes with phylogenetic origins from eukaryotes, implies a complex evolution for adaptation to obligate intracellular parasitism... - Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2J E Allen
Department of Biomedical and Environmental Health Science, University of California, Berkeley 94720
J Bacteriol 171:285-91. 1989..The mature proteins had a net positive charge and contained 24 cysteine residues... - Chlamydia outer membrane protein discovery using genomicsR S Stephens
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
Curr Opin Microbiol 4:16-20. 2001..Several of the predicted outer membrane proteins recently have been shown to be translated and localized to the surface of the chlamydial outer membrane... - Chlamydial genovar distribution after community wide antibiotic treatmentD L Bain
Division of Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA
J Infect Dis 184:1581-8. 2001..Evidence consistent with reinfection and persistence as mechanisms of communitywide continued presence of trachoma was provided by data for individuals infected with rare genovars... - Divergence without difference: phylogenetics and taxonomy of Chlamydia resolvedRichard S Stephens
University of California, Berkeley, USA
FEMS Immunol Med Microbiol 55:115-9. 2009.... - Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorBA Kubo
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
Microbiology 147:3135-40. 2001..These data demonstrate that PorB is a dicarboxylate-specific porin that may feed the chlamydial TCA cycle and provide chlamydiae with carbon and energy production intermediates... - Expression of Chlamydia pneumoniae polymorphic membrane protein family genesJ Grimwood
Francis I. Proctor Foundation, University of California, San Francisco, California, USA
Infect Immun 69:2383-9. 2001..Analyses of additional C. pneumoniae strains showed that although some Pmps are conserved, others vary between strains, in both molecular weight and level of expression... - Developmental regulation of tandem promoters for the major outer membrane protein gene of Chlamydia trachomatisR S Stephens
Department of Biomedical and Environmental Health Science, School of Public Health, University of California, Berkeley 94720
J Bacteriol 170:744-50. 1988..Mapping of the initiating nucleotide for each transcript suggested that this gene is regulated by differential transcription from tandem promoters... - Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatisBrian W Brunelle
Program in Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA
Genome Biol 5:R42. 2004..Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis... - Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomeraseStephanie Abromaitis
Program in Infectious Diseases and Immunity, University of California Berkeley, Berkeley, CA, USA
PLoS Pathog 5:e1000357. 2009.... - Cysteine-rich outer membrane proteins of Chlamydia trachomatis display compensatory sequence changes between biovariantsJ E Allen
Department of Biomedical and Environmental Health Sciences, University of California, Berkeley 94720
Mol Microbiol 4:1543-50. 1990..These compensatory charge alterations have implications for the outer membrane organization of these proteins. In addition, examination of the OMP3 sequence suggests that OMP3 may be a lipoprotein... - Chlamydial effector proteins localized to the host cell cytoplasmic compartmentBetsy Kleba
Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
Infect Immun 76:4842-50. 2008..These data confirm that a dynamic relationship exists between Chlamydia and its host and that translocation of bacterial proteins into the cytosol is developmentally dependent... - The cytosolic pattern recognition receptor NOD1 induces inflammatory interleukin-8 during Chlamydia trachomatis infectionKerry R Buchholz
Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720 7354, USA
Infect Immun 76:3150-5. 2008..Since ERK is not activated through this pathway, a concomitant interaction between the host and bacteria is additionally required for full activation of the endogenous IL-8 response... - The extracellular signal-regulated kinase/mitogen-activated protein kinase pathway induces the inflammatory factor interleukin-8 following Chlamydia trachomatis infectionKerry R Buchholz
Program in Infectious Diseases and Immunity, School of Public Health, University of California Berkeley, Berkeley, CA 94720, USA
Infect Immun 75:5924-9. 2007..C. trachomatis serovar D also induced IL-8 in an ERK-dependent manner. We concluded that IL-8 induced during infection of epithelial cells is dependent on continual activation of ERK by C. trachomatis... - Mechanisms of host cell exit by the intracellular bacterium ChlamydiaKevin Hybiske
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 104:11430-5. 2007..The participation of Rho was unique in that it functioned late in extrusion. The dual nature of release characterized for Chlamydia has not been observed as a strategy for intracellular bacteria... - Mechanisms of Chlamydia trachomatis entry into nonphagocytic cellsKevin Hybiske
Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California Berkeley, Berkeley, CA 94720, USA
Infect Immun 75:3925-34. 2007..Thus, clathrin and its coordinate accessory factors were required for entry of C. trachomatis, although additional, uncharacterized mechanisms are also utilized... - Selection of mutant cell lines resistant to infection by Chlamydia spp [corrected]Trevor Fudyk
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
Infect Immun 70:6444-7. 2002..Four distinct mutant cell phenotypes with defects in either attachment or postattachment mechanisms that are required for infection by C. trachomatis and Chlamydia pneumoniae were characterized... - Antigenic topology of chlamydial PorB protein and identification of targets for immune neutralization of infectivityDiane E Kawa
Division of Infectious Diseases, School of Public Health, 235 Warren Hall, University of California, Berkeley, CA 94720, USA
J Immunol 168:5184-91. 2002..trachomatis PorB antisera was inhibited by synthetic peptides representing the surface-exposed PorB antigenic determinants. These findings demonstrate that PorB Ags may be useful for development of chlamydial vaccines... - Immune response to the Chlamydia trachomatis outer membrane protein PorBDiane E Kawa
Program in Infectious Diseases, School of Public Health, University of California, 140 Earl Warren Hall, Berkeley, CA 94720, USA
Vaccine 22:4282-6. 2004.... - ChxR is a transcriptional activator in ChlamydiaIngrid Chou Koo
Program in Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
Proc Natl Acad Sci U S A 103:750-5. 2006..Both proteins could bind simultaneously to their nonoverlapping binding sites. This report identifies a stage-specific transcriptional regulator and some of its target genes in Chlamydia... - Phage-display antibody detection of Chlamydia trachomatis-associated antigensErika A Lindquist
Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA
Microbiology 148:443-51. 2002.... - Chlamydia pneumoniae encodes a functional aromatic amino acid hydroxylaseStephanie Abromaitis
University of California, Berkeley, 94720, USA
FEMS Immunol Med Microbiol 55:196-205. 2009..The maintenance of this gene within a number of Chlamydia suggests that the enzyme may have an important role in shaping the metabolism or overall pathogenesis of these bacteria... - A developmentally regulated two-component signal transduction system in ChlamydiaIngrid Chou Koo
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-7360, USA
J Biol Chem 278:17314-9. 2003..Collectively, these results show that CtcB and CtcC function as a two-component system and are likely responsible for transcriptional regulation by sigma(54) holoenzyme during late-stage chlamydial development... - Global stage-specific gene regulation during the developmental cycle of Chlamydia trachomatisTracy L Nicholson
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
J Bacteriol 185:3179-89. 2003..The correlation of these gene clusters with hallmark morphological events of the chlamydial developmental cycle suggests three global stage-specific networks of gene regulation... - Genetic diversity of Chlamydia trachomatis and the prevalence of trachomaJianzhi Zhang
Francis I Proctor Foundation, University of California, San Francisco, CA 94143-0944, USA
Pediatr Infect Dis J 23:217-20. 2004..CONCLUSION: Genetic diversity of C. trachomatis is associated with the prevalence of infection in a community, consistent with the hypothesis that diversity may be necessary to attain a high prevalence in a community... - Recruitment of mammalian cell fibronectin to the surface of Chlamydia trachomatisBetsy J Kleba
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
Infect Immun 70:3935-8. 2002..We also demonstrate that a heparan sulfate lyase-sensitive molecule on chlamydial EB is responsible for binding at least a portion of this fibronectin... - Chlamydia trachomatis lacks an adaptive response to changes in carbon source availabilityTracy L Nicholson
Program in Infectious Diseases, School of Public Health, University of California-Berkeley, 140 Earl Warren Hall, Berkeley, CA 94720-7360, USA
Infect Immun 72:4286-9. 2004..In contrast to free-living bacteria, changing the carbon source from glucose to glutamate or alpha-ketoglutarate had little effect on the global gene transcription of C. trachomatis... - Chlamydial type III secretion system is encoded on ten operons preceded by sigma 70-like promoter elementsP Scott Hefty
Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, Berkeley, CA 94720, USA
J Bacteriol 189:198-206. 2007..Transcriptional initiation by a sigma factor responsible for constitutive gene expression indicates that undefined activators or repressors regulate developmental stage-specific expression of chlamydial type III secretion system genes... - Bacteria-associated fibronectin does not enhance Chlamydia trachomatis infectivity in vitroBetsy Kleba
Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
Microb Pathog 39:53-5. 2005..However, at high fibronectin concentrations (1 mg/ml) bacterial infectivity was reduced. These data suggest that, unlike several other microbial pathogens, C. trachomatis does not utilize fibronectin to mediate infectivity in vitro... - Core of the partner switching signalling mechanism is conserved in the obligate intracellular pathogen Chlamydia trachomatisLei Hua
Department of Food Science and Technology, University of California, Davis, 95616, USA
Mol Microbiol 59:623-36. 2006..trachomatis cytoplasmic membrane... - Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolatesNicholas R Thomson
The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
Genome Res 18:161-71. 2008..We have used new sequencing technologies to show that the recent clinical LGV isolate causing proctitis is unlikely to be a newly emerged strain but is most probably an old strain with relatively new clinical manifestations...