Research Topics
Genomes and Genes | Vanessa SperandioSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
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Publications
Quorum sensing in Escherichia coli and SalmonellaMatthew Walters
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
Int J Med Microbiol 296:125-31. 2006....- CadA negatively regulates Escherichia coli O157:H7 adherence and intestinal colonizationRoberto C Vazquez-Juarez
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
Infect Immun 76:5072-81. 2008..In the infant rabbit model, the cadA mutant outcompeted the wild-type strain in the ileum but not in the cecum or mid-colon, raising the possibility that CadA negatively regulates EHEC pathogenicity in a tissue-specific fashion... - Hfq Virulence Regulation in Enterohemorrhagic Escherichia coli O157:H7 Strain 86-24Melissa M Kendall
University of Texas Southwestern Medical Center, Dept of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390 9048
J Bacteriol 193:6843-51. 2011..Altogether, these data indicate that Hfq plays a regulatory role in EHEC 86-24 that is different from what has been reported for EHEC strain EDL933 and that the role of Hfq in EHEC virulence regulation extends beyond the LEE... - SdiA sensing of acyl-homoserine lactones by enterohemorrhagic E. coli (EHEC) serotype O157:H7 in the bovine rumenVanessa Sperandio
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
Gut Microbes 1:432-5. 2010.... - Autoinducer 3 and epinephrine signaling in the kinetics of locus of enterocyte effacement gene expression in enterohemorrhagic Escherichia coliMatthew Walters
University of Texas Southwestern Medical Center, Dept of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390 9048
Infect Immun 74:5445-55. 2006..The results suggest a possible synergistic relationship between AI-3 and epinephrine. The combined effects of these two signaling molecules may lead to greater LEE expression and a more efficient infection... - Bacteria-host communication: the language of hormonesVanessa Sperandio
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Proc Natl Acad Sci U S A 100:8951-6. 2003.... - Novel approaches to bacterial infection therapy by interfering with bacteria-to-bacteria signalingVanessa Sperandio
University of Texas Southwestern Medical Center, Dept of Microbiology, 5325 Harry Hines Blvd, Dallas, TX 75390 9048, USA
Expert Rev Anti Infect Ther 5:271-6. 2007..Moreover, since QS is not involved directly in essential processes, such as bacterial growth, one can reason that inhibition of QS will not yield a selective pressure for the development of resistance... - Striking a balance: inter-kingdom cell-to-cell signaling, friendship or war?Vanessa Sperandio
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Trends Immunol 25:505-7. 2004.... - The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesisNicola C Reading
Department of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Proc Natl Acad Sci U S A 106:5889-94. 2009..QseG is an OM protein necessary for translocation of TTSS effectors that also works in conjunction with a 2-component signaling system that senses host stress signals... - Targeting QseC signaling and virulence for antibiotic developmentDavid A Rasko
Department of Microbiology, University of Texas UT Southwestern Medical Center, Dallas, TX 75390, USA
Science 321:1078-80. 2008..Inhibition of signaling offers a strategy for the development of broad-spectrum antimicrobial drugs... - Global effects of the cell-to-cell signaling molecules autoinducer-2, autoinducer-3, and epinephrine in a luxS mutant of enterohemorrhagic Escherichia coliMelissa M Kendall
University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9048, USA
Infect Immun 75:4875-84. 2007..This study began to examine the global signaling networks in EHEC and revealed expression profiles that are signal and pathogen specific... - The QseC adrenergic signaling cascade in Enterohemorrhagic E. coli (EHEC)David T Hughes
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
PLoS Pathog 5:e1000553. 2009..Understanding adrenergic regulation of a bacterial cell is a powerful approach for studying the underlying mechanisms of stress and cellular survival... - AI-3 synthesis is not dependent on luxS in Escherichia coliMatthew Walters
University of Texas Southwestern Medical Center, Department of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
J Bacteriol 188:5668-81. 2006..This study examines how AI-3 production is affected by the luxS mutation and explores the roles of the LuxS/AI-2 system in metabolism and QS... - QseA and GrlR/GrlA regulation of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coliRegan M Russell
University of Texas Southwestern Medical Center, Department of Microbiology, Dallas, TX 75390 9048, USA
J Bacteriol 189:5387-92. 2007..This work demonstrates that GrlR/GrlA activate LEE2 transcription in a Ler-independent fashion, whereas transcription of grlRA is activated by QseA in both Ler-dependent and -independent manners... - A transcriptome study of the QseEF two-component system and the QseG membrane protein in enterohaemorrhagic Escherichia coli O157 : H7Nicola C Reading
Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9048, USA
Microbiology 156:1167-75. 2010..The similarity of the microarray profiles of these mutants also indicates that these proteins work together. These data indicate that QseEFG are involved in the regulation of virulence and metabolism in EHEC... - QseC mediates Salmonella enterica serovar typhimurium virulence in vitro and in vivoCristiano G Moreira
University of Texas Southwestern Medical Center, Department of Microbiology, Dallas, TX 75390 9048, USA
Infect Immun 78:914-26. 2010..Elucidation of the role of this interkingdom signaling system in Salmonella serovar Typhimurium should contribute to a better understanding of the complex interplay between the pathogen and the host during infection... - A novel two-component signaling system that activates transcription of an enterohemorrhagic Escherichia coli effector involved in remodeling of host actinNicola C Reading
Dept of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
J Bacteriol 189:2468-76. 2007..These findings suggest the QseEF is a two-component system involved in the regulation of AE lesion formation by EHEC... - Novel approaches to bacterial infection therapy by interfering with cell-to-cell signalingDavid A Rasko
University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
Curr Protoc Microbiol . 2009..We also provide a detailed protocol using a specific example of signaling inhibitor identification using enterohemorrhagic Escherichia coli as a model system... - The LysR-type regulator QseA regulates both characterized and putative virulence genes in enterohaemorrhagic Escherichia coli O157:H7Melissa M Kendall
University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
Mol Microbiol 76:1306-21. 2010..Taken together, these results indicate that QseA acts as a global regulator in EHEC, co-ordinating expression of virulence genes... - Chemical sensing in mammalian host-bacterial commensal associationsDavid T Hughes
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 107:9831-6. 2010.... - Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coliMarcie B Clarke
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Mol Microbiol 57:1734-49. 2005..Taken together, these results indicate that transcriptional initiation at the flhDC promoter by QseBC appears to be complex and dependent on the presence of FliA (sigma28)... - Modulation of enteropathogenic Escherichia coli virulence by quorum sensingMarcelo P Sircili
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Infect Immun 72:2329-37. 2004..These results suggest that QS is involved in modulating the regulation of the EPEC virulence genes... - The QseC sensor kinase: a bacterial adrenergic receptorMarcie B Clarke
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Proc Natl Acad Sci U S A 103:10420-5. 2006..Given the role that this system plays in bacterial virulence, further characterization of this unique signaling mechanism may be important for developing novel classes of antimicrobials... - Inter-kingdom signalling: communication between bacteria and their hostsDavid T Hughes
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
Nat Rev Microbiol 6:111-20. 2008..Recent evidence shows, however, that quorum-sensing signalling is not restricted to bacterial cell-to-cell communication, but also allows communication between microorganisms and their hosts... - QseA directly activates transcription of LEE1 in enterohemorrhagic Escherichia coliFaith C Sharp
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
Infect Immun 75:2432-40. 2007..These studies suggest that QseA activates the transcription of LEE1 by directly binding upstream of its P1 promoter region... - Quorum sensing: the many languages of bacteriaNicola C Reading
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048, USA
FEMS Microbiol Lett 254:1-11. 2006..Three major quorum-sensing circuits have been described: one used primarily by Gram-negative bacteria, one used primarily by Gram-positive bacteria, and one that has been proposed to be universal... - Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E. coli (EHEC)Marcie B Clarke
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Mol Microbiol 58:441-55. 2005..These results indicate that QseB may act to autoregulate its own transcription through binding to low- and high-affinity sites found in its promoter... - Enterohemorrhagic Escherichia coli virulence regulation by two bacterial adrenergic kinases, QseC and QseEJacqueline Njoroge
Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
Infect Immun 80:688-703. 2012..These results genetically and phenotypically map the contributions of the two adrenergic sensors QseC and QseE to EHEC pathogenesis... - Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coliCristiano G Moreira
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
J Bacteriol 188:3952-61. 2006..Taken together, these studies allowed us to propose a model of EPEC biofilm formation... - The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coliBenjamin J Habdas
University of Texas Southwestern Medical Center, Department of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
J Bacteriol 192:3699-712. 2010..The LTTRs known to regulate LEE transcription, QseA and LrhA, do not interact with sQseD, suggesting that sQseD acts as a dominant-negative partner with a yet-unidentified LTTR... - Jamming bacterial communication: new approaches for the treatment of infectious diseasesJacqueline Njoroge
Department of Microbiology, UT Southwestern Medical Center, Dallas, TX, USA
EMBO Mol Med 1:201-10. 2009..QS inhibitors (QSIs) hold great expectations and we look forward to their application in fighting bacterial infections... - Quorum sensing by enteric pathogensMelissa M Kendall
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Curr Opin Gastroenterol 23:10-5. 2007.... - An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio choleraeJeongmi Lee
Departments of Pharmacology, Microbiology, and Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
J Biol Chem 284:9899-907. 2009..cholerae infection. Notably, the alternative polyamine biosynthetic pathway is widespread in bacteria and is likely to play a previously unrecognized role in the biology of these organisms... - Events at the host-microbial interface of the gastrointestinal tract III. Cell-to-cell signaling among microbial flora, host, and pathogens: there is a whole lot of talking going onMarcie B Clarke
Univ. of Texas Southwestern Medical Center, Dept. of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390-9048, USA
Am J Physiol Gastrointest Liver Physiol 288:G1105-9. 2005..This review explores the cell-to-cell signaling events in the gastrointestinal tract that lead EHEC to regulate its virulence genes in a coordinate manner... - Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmidsDavid A Rasko
University of Texas Southwestern Medical Center at Dallas, Department of Microbiology, 6000 Harry Hines Blvd, Room NA6 138, Dallas, TX 75235 9048, USA
Plasmid 58:159-66. 2007..tularensis genetics. Our promoter trap can identify promoters that are activated during intracellular growth and survival. These new vectors provide additional tools for the genetic manipulation of F. tularensis... - Cell-to-cell signalling during pathogenesisChristopher T Parker
University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
Cell Microbiol 11:363-9. 2009..This article explores the role of these four signalling systems in bacterial communities and how pathogens use these systems to control genes required during host invasion and infection... - Colonization of gnotobiotic piglets by a luxS mutant strain of Escherichia coli O157:H7Dianna M Jordan
Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA 50011, USA
Infect Immun 73:1214-6. 2005.... - Quorum-sensing Escherichia coli regulator A: a regulator of the LysR family involved in the regulation of the locus of enterocyte effacement pathogenicity island in enterohemorrhagic E. coliVanessa Sperandio
Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Infect Immun 70:3085-93. 2002..Similar results were also observed with a qseA mutant of EPEC. The QseA regulator is part of the regulatory cascade that regulates EHEC and EPEC virulence genes by quorum sensing... - Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coliVanessa Sperandio
Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 West Baltimore Street, Baltimore, MD 21201, USA
Mol Microbiol 43:809-21. 2002.... - Bacterial cell-to-cell signaling in the gastrointestinal tractJames B Kaper
Center for Vaccine Development, University of Maryland School of Medicine, 685 W. Baltimore St, Baltimore, MD 21201, USA
Infect Immun 73:3197-209. 2005 - Quorum sensing in Pseudomonas aeruginosa: yet another playerVanessa Sperandio
Trends Microbiol 10:118. 2002 - The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolatesDavid A Rasko
Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, 20 Penn Street, Baltimore, MD 21201, USA
J Bacteriol 190:6881-93. 2008..This comparative study of the species E. coli, while descriptive, should provide the basis for future functional work on this important group of pathogens...
Research Grants
- Quorum sensing regulation of EHEC virulence genesVanessa Sperandio; Fiscal Year: 2003....
- Quorum sensing regulation of EHEC virulence genesVanessa Sperandio; Fiscal Year: 2010....
- Quorum sensing regulation of EHEC virulence genesVanessa Sperandio; Fiscal Year: 2007..abstract_text> ..
- Screen for Quorum Sensing Molecular Inhibitors (RMI)Vanessa Sperandio; Fiscal Year: 2005....
- Quorum sensing regulation of EHEC virulence genesVanessa Sperandio; Fiscal Year: 2006..abstract_text> ..
- EHEC interactions with the normal intestinal floraVanessa Sperandio; Fiscal Year: 2004..In Specific Aim 2, we propose to monitor EHEC virulence gene expression in a mixed population in the presence of the intestinal flora. ..
- SdiA regulation of EHEC virulenceVanessa Sperandio; Fiscal Year: 2010....