Research Topics
Genomes and Genes | J P SimmerSummaryAffiliation: University of Texas Health Science Center Country: USA Publications
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Detail Information
Publications
Developmental biology and genetics of dental malformationsJan C C Hu
Department of Orthodontics and Pediatric Dentistry, University of Michigan School of Dentistry, Ann Arbor, MI, USA
Orthod Craniofac Res 10:45-52. 2007....- Dental enamel formation and its impact on clinical dentistryJ P Simmer
Department of Pediatric Dentistry at the University of Texas Health Science Center at San Antonio, 78229 3900, USA
J Dent Educ 65:896-905. 2001..Here we review our current understanding of dental enamel formation, and relate this information to clinical circumstances where this understanding may be particularly relevant... - Characterization of the mouse and human PRSS17 genes, their relationship to other serine proteases, and the expression of PRSS17 in developing mouse incisorsJ C Hu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry, 78284 7888, USA
Gene 251:1-8. 2000..This pattern supports a role for the PRSS17 protein in the degradation of enamel proteins... - Expression, structure, and function of enamel proteinasesJames P Simmer
University of Texas Health Science Center at San Antonio, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
Connect Tissue Res 43:441-9. 2002..A brief review of dental enamel formation is presented, followed by a more detailed analysis of enamelysin and KLK4 expression, structure, and function... - Localization of EMSP1 expression during tooth formation and cloning of mouse cDNAJ C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 78284 7888, USA
J Dent Res 79:70-6. 2000..We conclude that EMSP1 is expressed by pig and mouse ameloblasts during the early maturation stage of amelogenesis... - Cloning human enamelin cDNA, chromosomal localization, and analysis of expression during tooth developmentC C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 78284 7888, USA
J Dent Res 79:912-9. 2000..These findings will facilitate the search for specific mutations in the enamelin gene in kindreds suffering from amelogenesis imperfecta... - Cloning and characterization of porcine enamelin mRNAsC C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 76:1720-9. 1997..Intact enamelin and proteolytic cleavage products containing its carboxyl terminus are limited to the most superficial layer of the developing enamel matrix, while other enamelin cleavage products are observed in deeper enamel... - Murine enamelin: cDNA and derived protein sequencesC C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 78284 7888, USA
Connect Tissue Res 39:47-61; discussion 63-7. 1998..Mouse and porcine enamelin share 61% amino acid identity and 75% DNA sequence identity. Mouse enamelin has 14 tandemly arranged copies of an 11 amino acid segment that is found only once in porcine enamelin... - Purification, characterization, and cloning of enamel matrix serine proteinase 1J P Simmer
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 77:377-86. 1998..Gelatin enzymography shows a dramatic increase in EMSP1 activity in the transition-stage enamel matrix. EMSP1 is most homologous to kallikriens and trypsins... - Sheathlin: cloning, cDNA/polypeptide sequences, and immunolocalization of porcine enamel sheath proteinsC C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 76:648-57. 1997..We propose that the porcine sheath proteins and their proteolytic cleavage products be designated "sheathlin"... - Cloning, characterization, and heterologous expression of exon-4-containing amelogenin mRNAsC C Hu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 76:641-7. 1997..The exon-4-containing amelogenin isoforms were heterologously expressed in E. coli by means of the pET11 expression system (Novagen, Madison, WI)... - Cloning and characterization of the mouse and human enamelin genesJ C Hu
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 78229 3900, USA
J Dent Res 80:898-902. 2001.... - Cloning, characterization, and tissue expression pattern of mouse tuftelin cDNAM MacDougall
University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 78284 7888, USA
J Dent Res 77:1970-8. 1998..6 and 3.2 kb, were detected. DNA sequence characterization of an RT-PCR amplification product confirmed expression of tuftelin in kidney, and identified an alternatively spliced mouse tuftelin mRNA lacking exon 2... - Cloning, cDNA sequence, and alternative splicing of porcine amelogenin mRNAsC C Hu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 75:1735-41. 1996..The 173- and the 56-residue amelogenins can be expressed from either promoter, while the 157-residue amelogenin is generated by only one of the two promoters... - Pig amelogenin gene expresses a unique exon 4C C Hu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284 7888, USA
Connect Tissue Res 43:435-40. 2002..We conclude that the pig amelogenin gene expresses a unique exon 4 that is not homologous to, or evolved from, the exon 4 segment expressed in humans and rodents... - Alternative splicing of the mouse amelogenin primary RNA transcriptJ P Simmer
University of Texas School of Dentistry, Department of Pediatric Dentistry, San Antonio 78284 7888
Calcif Tissue Int 55:302-10. 1994..Antibodies were raised to synthetic exon 4-encoded polypeptides and used to immunostain Western transfers and histologic tooth sections... - Cloning, DNA sequence, and alternative splicing of opossum amelogenin mRNAsC C Hu
University of Texas Health Science Center at San Antonio, Department of Pediatric Dentistry 78284 7888, USA
J Dent Res 75:1728-34. 1996.... - A comparison of enamelin and amelogenin expression in developing mouse molarsJ C Hu
University of Texas Health Science Center at San Antonio, 78229-3900, USA
Eur J Oral Sci 109:125-32. 2001..No expression was observed in pulp, bone, or along the developing root. We conclude that amelogenin and enamelin are enamel-specific and do not directly participate in the formation of dentin or cementum in developing mouse molars... - Calcium binding of enamel proteins and their derivatives with emphasis on the calcium-binding domain of porcine sheathlinY Yamakoshi
Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2 1 3 Tsurumi, Tsurumi ku, 230 8501, Yokohama, Japan
Arch Oral Biol 46:1005-14. 2001..3 kDa amelogenins. It is concluded that if enamel proteins have similar calcium-binding properties in vivo as have been measured in vitro, they would tend to buffer the free calcium ion concentration in enamel fluid... - Alternative splicing of amelogeninsJ P Simmer
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry 78284 7888, USA
Connect Tissue Res 32:131-6. 1995..These data indicate that alternatively spliced amelogenin mRNAs are translated into protein and secreted into the enamel matrix... - Enamelysin (matrix metalloproteinase 20)-deficient mice display an amelogenesis imperfecta phenotypeJohn J Caterina
Matrix Metalloproteinase Unit, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 277:49598-604. 2002..Our findings demonstrate that enamelysin activity is essential for proper enamel development... - Conservation and variation in enamel protein distribution during vertebrate tooth developmentPaul G Satchell
Baylor College of Dentistry/Texas A and M University System, Dallas, Texas, USA
J Exp Zool 294:91-106. 2002..We speculate that there might be a unique correlation between amelogenin-rich tetrapod and lungfish enamel with long and parallel crystals and enamelin-rich basal vertebrate enameloid with diverse patterns of crystal organization... - Enamelysin and kallikrein-4 mRNA expression in developing mouse molarsJan C C Hu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry, 78229 3900, USA
Eur J Oral Sci 110:307-15. 2002..the KLK4 signal was present in maturation-stage ameloblasts on days 9, 11, and 14. The expression patterns of MMP-20 and KLK4 by ameloblasts in mouse molars are stage-specific and complementary... - Porcine kallikrein-4 activation, glycosylation, activity, and expression in prokaryotic and eukaryotic hostsOkhee Ryu
University of Texas School of Dentistry, Health Science Center at San Antonio, Department of Pediatric Dentistry, San Antonio, Texas, USA
Eur J Oral Sci 110:358-65. 2002..Eleven cleavage sites in the amelogenin protein were identified, demonstrating that KLK4 degrades amelogenin and is likely to participate in the degradation of enamel proteins in vivo... - How do enamelysin and kallikrein 4 process the 32-kDa enamelin?Yasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:45-51; discussion 93-5, 379-80. 2006..After 12 h of digestion with KLK4, all of the 32-kDa enamelin had been cleaved, but some cleavage products persisted after 48 h of digestion... - Mutational analysis of candidate genes in 24 amelogenesis imperfecta familiesJung-Wook Kim
University of Michigan School of Dentistry, University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA, and Department of Pediatric Dentistry and Dental Research Institute, Seoul, Korea
Eur J Oral Sci 114:3-12; discussion 39-41, 379. 2006..Out of 24 families with isolated enamel defects, only six disease-causing mutations were identified in the AI candidate genes. This finding suggests that many additional genes potentially contribute to the etiology of AI... - Enamel formation and amelogenesis imperfectaJan C C Hu
University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
Cells Tissues Organs 186:78-85. 2007..In addition, we provide a fresh perspective on the roles matrix proteins play in catalyzing the biomineralization of dental enamel... - pH triggered self-assembly of native and recombinant amelogenins under physiological pH and temperature in vitroFelicitas B Wiedemann-Bidlack
Department of Biomineralization, The Forsyth Institute, 140 The Fenway, Boston, MA 02115 3782, USA
J Struct Biol 160:57-69. 2007..Hence, tightly controlled pH values during secretory amelogenesis may serve to regulate the functions of both full-length and cleaved amelogenins... - FAM83H mutations in families with autosomal-dominant hypocalcified amelogenesis imperfectaJung Wook Kim
Department of Cell and Developmental Biology, Dental Research Institute, School of Dentistry, Seoul National University, 275 1 Yongon dong, Chongno Gu, Seoul 110 768, Korea
Am J Hum Genet 82:489-94. 2008..3. The mutations perfectly cosegregate with the disease phenotype and demonstrate that FAM83H is required for proper dental-enamel calcification... - Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in miceJan C C Hu
Department of Orthodontics and Pediatric Dentistry and Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48108, USA
J Biol Chem 283:10858-71. 2008..These results demonstrate ameloblast-specific expression of enamelin and reveal that enamelin is essential for proper enamel matrix organization and mineralization... - Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam)Yuanyuan Hu
Department of Orthodontics and Pediatric Dentistry, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
Eur J Oral Sci 116:113-23. 2008..Our study provides new insights into regulatory mechanisms governing enamelin expression... - Porcine dentin sialophosphoprotein: length polymorphisms, glycosylation, phosphorylation, and stabilityYasuo Yamakoshi
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
J Biol Chem 283:14835-44. 2008..The 4 alleles encode DPP domains having 551, 575, 589, or 594 amino acids and completely explain the DPP size variations. DPP length variations are polymorphic and are not associated with dentin defects... - Co-operative mineralization and protein self-assembly in amelogenesis: silica mineralization and assembly of recombinant amelogenins in vitroChristabel E Fowler
GlaxoSmith Kline, Weybridge, Surrey, UK
Eur J Oral Sci 114:297-303; discussion 327-9, 382. 2006..The results also suggest that full-length amelogenin can guide the formation of organized mineralized structures through co-operative interactions between assembling protein and forming mineral... - Proteomic analysis of enamel matrix using a two-dimensional protein fractionation systemYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:266-71; discussion 285-6, 382. 2006..Loss of these glycoproteins is associated with a post-transition increase in enamel mineralization... - Porcine SPARC: isolation from dentin, cDNA sequence, and computer modelYong-Hee P Chun
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:78-85; discussion 93-5, 379-80. 2006..SPARC is composed of three structural/functional domains: an acidic Ca2+-binding, a follistatin-like, and an extracellular calcium-binding domain... - Proteomics and genetics of dental enamelJan C-C Hu
University of Michigan Dental Research Lab, Ann Arbor, Mich. 48108, USA
Cells Tissues Organs 181:219-31. 2005.... - MMP-20 is predominately a tooth-specific enzyme with a deep catalytic pocket that hydrolyzes type V collagenBenjamin E Turk
Department of Pharmacology, Yale University, New Haven, Connecticut 06520, USA
Biochemistry 45:3863-74. 2006.... - Porcine dentin matrix protein 1: gene structure, cDNA sequence, and expression in teethJung Wook Kim
University of Michigan Dental Research Laboratory, Ann Arbor, 48108, USA
Eur J Oral Sci 114:33-41. 2006..In developing teeth, immunohistochemistry localized DMP1 in odontoblasts and the dentinal tubules of mineralized dentin and in ameloblasts, but not in the enamel matrix... - Thermal denaturation of a recombinant mouse amelogenin: circular dichroism and differential scanning calorimetric studiesMotohisa Oobatake
Protein Engineering Research Institute, Osaka, Japan
Proteins 62:461-9. 2006..The thermodynamic characteristics of rM179 are unique and different from a typical globular protein, proline-rich peptides, and a molten globule state... - Dentin glycoprotein: the protein in the middle of the dentin sialophosphoprotein chimeraYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, Michigan 48108, USA
J Biol Chem 280:17472-9. 2005.... - The effect of recombinant mouse amelogenins on the formation and organization of hydroxyapatite crystals in vitroElia Beniash
Department of Biomineralization, The Forsyth Institute, Boston, MA 02115 3799, USA
J Struct Biol 149:182-90. 2005..Our data also suggest that nascent enamel structure emerges as a result of cooperative interactions between forming crystals and assembling proteins... - Kallikrein 4 is a secreted proteinJames P Simmer
Cancer Res 64:8481-2; author reply 8482-3. 2004 - Functions of KLK4 and MMP-20 in dental enamel formationYuhe Lu
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109 1078, USA
Biol Chem 389:695-700. 2008....