Research Topics
| ROBERT M SHANKSSummaryAffiliation: University of Pittsburgh Country: USA Publications
Research Grants
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Detail Information
Publications
Development of a novel system for isolating genes involved in predator-prey interactions using host independent derivatives of Bdellovibrio bacteriovorus 109JAdrian A Medina
Department of Oral Biology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07101, USA
BMC Microbiol 8:33. 2008..Although the life cycle of Bdellovibrio has been extensively investigated, very little is known about the mechanisms involved in predation...- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesisJoseph Horzempa
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
BMC Microbiol 8:172. 2008..After infecting a mammalian host, the facultative intracellular bacterium, Francisella tularensis, encounters an elevated environmental temperature. We hypothesized that this temperature change may regulate genes essential for infection... - New yeast recombineering tools for bacteriaRobert M Q Shanks
Department of Ophthalmology, University of Pittsburgh Eye Center, PA 15213, USA
Plasmid 62:88-97. 2009..As a demonstration of utility, some of the approaches and vectors generated in this study were used to make a pigP deletion mutation in the opportunistic pathogen Serratia marcescens... - A Serratia marcescens OxyR homolog mediates surface attachment and biofilm formationRobert M Q Shanks
Charles T Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, UPMC Eye Center, Pittsburgh, PA 15213, USA
J Bacteriol 189:7262-72. 2007..Together, these data support a model in which OxyR contributes to early stages of S. marcescens biofilm formation by influencing fimbrial gene expression... - The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriaeEric J Kalivoda
Charles T Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
Appl Environ Microbiol 74:3461-70. 2008..The regulatory pathway by which environmental carbon sources influence cAMP concentrations to alter production of type 1 fimbrial adhesins establishes a novel mechanism by which bacteria control biofilm development... - Catabolite repression control of flagellum production by Serratia marcescensNicholas A Stella
Charles T Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Eye Center, EEI 1020, 203 Lothrop Street, Pittsburgh, PA 15213, USA
Res Microbiol 159:562-8. 2008..Given the role of flagella in bacterial pathogenicity, the regulatory pathway described here may assist us in better understanding the putative role of motility in dissemination and virulence of this opportunistic pathogen... - Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitisPaul P Thompson
The Charles T Campbell Ophthalmic Microbiology Laboratory, Ophthalmology and Visual Sciences Research Center, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Clin Microbiol 46:3232-6. 2008..Polyhexamethylene biguanide can inhibit PCR, and we suggest that specimen collection occur prior to topical treatment to avoid possible false-negative results... - CAP37-derived antimicrobial peptides have in vitro antiviral activity against adenovirus and herpes simplex virus type 1Y Jerold Gordon
The Charles T Campbell Lab Ophthalmic Microbiology Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Curr Eye Res 34:241-9. 2009..The antiviral activity of an established antibacterial CAP37 domain and its extracellular mechanism of action were investigated... - Topical levofloxacin 1.5% overcomes in vitro resistance in rabbit keratitis modelsRegis P Kowalski
Department of Ophthalmology Microbiology, The Eye and Ear Institute of Ophthalmology Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA
Acta Ophthalmol 88:e120-5. 2010..To determine whether topical levofloxacin 1.5% will successfully treat both levofloxacin-resistant and susceptible Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) in rabbit keratitis models... - Cyclic AMP negatively regulates prodigiosin production by Serratia marcescensEric J Kalivoda
Charles T Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Eye Center, Pittsburgh, PA 15213, USA
Res Microbiol 161:158-67. 2010..These experiments establish cAMP as a negative regulator of prodigiosin production in S. marcescens... - Francisella tularensis DeltapyrF mutants show that replication in nonmacrophages is sufficient for pathogenesis in vivoJoseph Horzempa
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
Infect Immun 78:2607-19. 2010..These findings indicate that replication in nonmacrophages contributes to the pathogenesis of F. tularensis... - Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensisJoseph Horzempa
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
J Microbiol Methods 80:106-8. 2010..For counterselection, we adapted a strategy using I-SceI, which causes a double-stranded break in the integrated suicide vector, forcing a second recombination to mediate allelic replacement... - A novel cell-associated protection assay demonstrates the ability of certain antibiotics to protect ocular surface cell lines from subsequent clinical Staphylococcus aureus challengeJ B Wingard
UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15208, USA
Antimicrob Agents Chemother 55:3788-94. 2011..A range of antibiotic effectiveness was displayed in this cell association assay, providing data that may be considered in addition to traditional testing when determining therapeutic dosing regimens... - Genetic evidence for an alternative citrate-dependent biofilm formation pathway in Staphylococcus aureus that is dependent on fibronectin binding proteins and the GraRS two-component regulatory systemRobert M Q Shanks
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
Infect Immun 76:2469-77. 2008..aureus catheter models of infection, we propose that the citrate-stimulated biofilm formation pathway may represent a clinically relevant pathway for the formation of these bacterial communities on medical implants... - Saccharomyces cerevisiae-based molecular tool kit for manipulation of genes from gram-negative bacteriaRobert M Q Shanks
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
Appl Environ Microbiol 72:5027-36. 2006..These tools can be used for genetic studies of pseudomonads and many other gram-negative bacteria... - Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfacesRobert M Q Shanks
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
Nephrol Dial Transplant 21:2247-55. 2006..A novel and potential clinically relevant finding of the present study is the observation that citrate at low levels strongly stimulates biofilm formation by S. aureus... - Rhamnolipids modulate swarming motility patterns of Pseudomonas aeruginosaNicky C Caiazza
Department of Microbiology and Immunology, Rm. 505, Vail Building, Dartmouth Medical School, Hanover, NH 03755, USA
J Bacteriol 187:7351-61. 2005..The abilities to produce and respond to rhamnolipids in the context of group behavior are discussed... - Heparin stimulates Staphylococcus aureus biofilm formationRobert M Q Shanks
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
Infect Immun 73:4596-606. 2005..These finding suggests that heparin stimulates S. aureus biofilm formation via a novel pathway...
Research Grants
- Novel virulence and exoenzyme regulatorsROBERT M SHANKS; Fiscal Year: 2010..The answers to these questions will allow for the creation of new drugs to treat S. marcescens ocular infections and reduce infection-associated vision loss. ..