Research Topics
| M J SadowskySummaryAffiliation: University of Minnesota Country: USA Publications
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Publications
AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymesM J Sadowsky
Department of Microbiology, Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St Paul 55108, USA
J Bacteriol 180:152-8. 1998..Overall, the data suggest that AtzA, AtzB, and AtzC diverged from a common ancestor and, by random events, have been reconstituted onto an atrazine catabolic plasmid...
A manganese-dependent dioxygenase from Arthrobacter globiformis CM-2 belongs to the major extradiol dioxygenase familyY R Boldt
Department of Microbiology, University of Minnesota, St Paul 55108
J Bacteriol 177:1225-32. 1995..The suggested common ancestry is also supported by sequence obtained from genes flanking mndD, which share significant sequence identity with xylJ and xylG from Pseudomonas putida...
Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally differentJ L Seffernick
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St. Paul, Minnesota 55108, USA
J Bacteriol 183:2405-10. 2001....
Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas sp. strain ADPB Martinez
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St Paul, Minnesota 55108, USA
J Bacteriol 183:5684-97. 2001..These results begin to provide insight into how plasmids are structured, and thus evolve, to encode the catabolism of compounds recently added to the biosphere...
The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathwayK L Boundy-Mills
Department of Soil, Water, and Climate, University of Minnesota, St Paul 55108, USA
Appl Environ Microbiol 63:916-23. 1997..The atzA and atzB genes catalyze the first two steps of the metabolic pathway in a bacterium that rapidly metabolizes atrazine to carbon dioxide, ammonia, and chloride...
Biodegradation of atrazine and related s-triazine compounds: from enzymes to field studiesL P Wackett
Department of Biochemistry, Molecular Biology and Biophysics and BioTechnology Institute, University of Minnesota, St Paul 55108, USA
Appl Microbiol Biotechnol 58:39-45. 2002..The method was used for cleaning up a spill of 1,000 pounds of atrazine to attain a level of herbicide acceptable to regulatory agencies...
Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazineM L De Souza
Department of Biochemistry, University of Minnesota, St Paul 55108, USA
Appl Environ Microbiol 61:3373-8. 1995..strain ADP. A 0.6-kb ApaI-PstI fragment from pMD4, containing the putative atrazine chlorohydrolase gene, hybridized to DNA from atrazine-degrading bacteria isolated in Switzerland and Louisiana.(ABSTRACT TRUNCATED AT 250 WORDS)..
The Bradyrhizobium japonicum noeD gene: a negatively acting, genotype-specific nodulation gene for soybeanS M Lohrke
Department of Soil, Water, and Climate, Biological Process Technology Institute, University of Minnesota, St Paul 55108, USA
Mol Plant Microbe Interact 11:476-88. 1998..These results suggest that specific LCO signal molecules may be one of the factors influencing nodulation specificity in this symbiotic system...
Evolution of catabolic pathways: Genomic insights into microbial s-triazine metabolismN Shapir
Department of Biochemistry, Molecular Biology, and Biophysics and BioTechnology Institute, University of Minnesota, 1479 Gortner Avenue, St. Paul, MN 55108, USA
J Bacteriol 189:674-82. 2007
Substrate specificity of atrazine chlorohydrolase and atrazine-catabolizing bacteriaJ L Seffernick
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St Paul, Minnesota 55108, USA
Appl Environ Microbiol 66:4247-52. 2000..strain ADP. This suggests that either (i) another enzyme(s) is present which confers the broader substrate range or (ii) the AtzA itself has a broader substrate range...
Therapeutic transplantation of the distal gut microbiotaA Khoruts
Department of Medicine, Center for Immunology, University of Minnesota, Minneapolis, Minnesota, USA
Mucosal Immunol 4:4-7. 2011....
Manganese(II) active site mutants of 3,4-dihydroxyphenylacetate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2Y R Boldt
Department of Microbiology, Center for Metals in Biocatalysis, University of Minnesota, Minneapolis 55455, USA
Biochemistry 36:2147-53. 1997..As is the case with the superoxide dismutases, the extradiol-cleaving catechol dioxygenases appear to utilize identical coordinating residues for their iron- and manganese-dependent enzymes...
Sphingobacterium sp. strain PM2-P1-29 harbours a functional tet(X) gene encoding for the degradation of tetracyclineS Ghosh
Department of Civil Engineering, University of Minnesota, Minneapolis, 55455 0116, USA
J Appl Microbiol 106:1336-42. 2009..In this study, we characterized a tetracycline resistant, aerobic, Gram-negative Sphingobacterium sp. strain PM2-P1-29 that harbours a tet(X) gene...
The Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeansM J Sadowsky
Soil Science Department, University of Minnesota, St Paul 54108
Proc Natl Acad Sci U S A 88:637-41. 1991..Restriction fragments that hybridize to pMJS12 were detected in genomic DNAs from both nodulation-restricted and -unrestricted strains...
Characterization of cytochromes c550 and c555 from Bradyrhizobium japonicum: cloning, mutagenesis, and sequencing of the c555 gene (cycC)R E Tully
Soybean and Alfalfa Research Laboratory, U S Department of Agriculture, Beltsville, Maryland 20705
J Bacteriol 173:7887-95. 1991..The mutant and wild type exhibited identical phenotypes when used to nodulate plants of soybean (Glycine max L. Merr.), with no significant differences in nodule number, nodule mass, or total amount of N2 fixed...
