Research Topics
| NICHOLAS R RHINDSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
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Detail Information
Publications
Comparative functional genomics of the fission yeastsNicholas Rhind
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Science 332:930-6. 2011..These analyses elucidate the genome structure and gene regulation of fission yeast and provide tools for investigation across the Schizosaccharomyces clade...- Reconciling stochastic origin firing with defined replication timingNicholas Rhind
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Chromosome Res 18:35-43. 2010..In addition, we propose biochemically plausible mechanisms for these criteria and point out how stochastic and defined origin firing can be experimentally distinguished in population experiments... - The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpointNicholas Willis
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genetics 184:887-97. 2010..Genetic interactions between MRN and Rhp51, the fission yeast Rad51 homolog, lead us to suggest that MRN participates in checkpoint-dependent replication slowing through negative regulation of recombination... - Regulation of DNA replication by the S-phase DNA damage checkpointNicholas Willis
Biochemistry and Molecular Pharmacology, University on Massachusetts Medical School, Worcester MA 01605, USA
Cell Div 4:13. 2009.... - DNA replication timing: random thoughts about origin firingNicholas Rhind
Biochemistry and Molecular Pharmacology Department, University of Massachusetts Medical School, 364 Plantation Street, LRB904, Worcester, MA 01605, USA
Nat Cell Biol 8:1313-6. 2006..This model assumes varying origin efficiency instead of a strict origin-timing programme. Here, we discuss the evidence for both models... - Changing of the guard: how ATM hands off DNA double-strand break signaling to ATRNicholas Rhind
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Mol Cell 33:672-4. 2009.... - An intrinsic checkpoint model for regulation of replication originsNicholas Rhind
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cell Cycle 7:2619-20. 2008..This coupling fails in a checkpoint mutant background because stalled forks disassemble and release replisome factors prematurely, allowing for unregulated origin firing... - The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signalingMary E Porter-Goff
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Biol Cell 20:2096-107. 2009..This observation leads us to conclude that other functions of MRN, possibly its role in replication fork metabolism, are required for S-phase DNA damage checkpoint function... - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpointNicholas Willis
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Biol Cell 20:819-33. 2009..We propose that restraining recombination is required for the slowing of replication in response to DNA damage... - The Hsk1(Cdc7) replication kinase regulates origin efficiencyPrasanta K Patel
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Biol Cell 19:5550-8. 2008..By manipulating Hsk1-Dfp1 levels, we show that increasing or decreasing origin firing rates leads to an increase in genomic instability, demonstrating the biological importance of appropriate origin efficiency... - DNA replication origins fire stochastically in fission yeastPrasanta K Patel
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Biol Cell 17:308-16. 2006..Thus, the fission yeast strategy for the initiation of replication is different from models of eukaryotic replication that propose coordinated origin firing... - The DNA replication checkpoint directly regulates MBF-dependent G1/S transcriptionChaitali Dutta
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell Biol 28:5977-85. 2008..Furthermore, the structural and regulatory similarity between MBF and E2F, the metazoan G(1)/S transcription factor, suggests that this checkpoint mechanism may be broadly conserved among eukaryotes... - The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stressChaitali Dutta
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
PLoS ONE 4:e6944. 2009..These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint... - Cdc2 tyrosine phosphorylation is not required for the S-phase DNA damage checkpoint in fission yeastNaveen Kommajosyula
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01609, USA
Cell Cycle 5:2495-500. 2006..Our results are consistent with a strictly Cdc2-Y15 phosphorylation-independent mechanism of the fission yeast S-phase DNA damage checkpoint... - Incorporation of thymidine analogs for studying replication kinetics in fission yeastNicholas Rhind
Biochemistry and Molecular Pharmacology Department, University of Massachusetts Medical School, Worcester, MA, USA
Methods Mol Biol 521:509-15. 2009..This chapter describes the labeling of fission yeast, Schizosaccharomyces pombe, with the thymidine analog BrdU in order to identify sites and determine kinetics of DNA replication... - A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombeAlla Sigova
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 18:2359-67. 2004..Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways... - In vivo labeling of fission yeast DNA with thymidine and thymidine analogsSasirekha Sivakumar
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Methods 33:213-9. 2004....
Research Grants
- The Role of MRN in the S-Phase DNA Damage CheckpointNicholas Rhind; Fiscal Year: 2009..This understanding will lead to new therapeutic targets and diagnostic tools for the treatment and prevention of human cancer. ..
- Mechanism of the S-Phase DNA Damage CheckpointNICHOLAS R RHIND; Fiscal Year: 2010..The proposed research will elucidate the function of this checkpoint, allowing for the identification of new therapeutic targets and diagnostic tools for the treatment and prevention of human cancer. ..