Research Topics
Genomes and Genes
| K PalczewskiSummaryAffiliation: University of Washington Country: USA Publications
| Collaborators
|
Detail Information
Publications
Rhodopsin: a structural primer for G-protein coupled receptorsRonald E Stenkamp
Department of Biochemistry, University of Washington, Seattle, 98195, USA
Arch Pharm (Weinheim) 338:209-16. 2005..A general view of these structural features is presented here...- Crystal structure of rhodopsin: A G protein-coupled receptorK Palczewski
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
Science 289:739-45. 2000..Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation... - Ca(2+)-binding proteins in the retina: structure, function, and the etiology of human visual diseasesK Palczewski
Department of Ophthalmology, University of Washington, Seattle, WA 98195 6485, USA
Bioessays 22:337-50. 2000..Understanding these Ca(2+)-binding proteins will provide a more complete picture of visual transduction, including the mechanisms associated with adaptation, and of related degenerative diseases... - Guanylate cyclase-activating proteins: structure, function, and diversityKrzysztof Palczewski
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
Biochem Biophys Res Commun 322:1123-30. 2004..In the absence of GCAP2, GCAP1 supports the generation of wild-type flash responses in both rod and cone cells. Recent progress revealed an unexpected complexity of the GC-GCAP system, pointing, out a number of unsolved questions... - Kinetics of visual pigment regeneration in excised mouse eyes and in mice with a targeted disruption of the gene encoding interphotoreceptor retinoid-binding protein or arrestinK Palczewski
Department of Ophthalmology, University of Washington, Seattle 98195, USA
Biochemistry 38:12012-9. 1999..Thus, the absence of arrestin appeared to have a minor effect on the kinetics of the visual cycle... - Activation and inactivation steps in the visual transduction pathwayK Palczewski
Department of Ophthalmology, University of Washington, Box 356485, Seattle, Washington 98195 6485, USA
Curr Opin Neurobiol 7:500-4. 1997.... - GTP-binding-protein-coupled receptor kinases--two mechanistic modelsK Palczewski
Department of Ophthalmology, University of Washington, School of Medicine, Seattle 98195 6485, USA
Eur J Biochem 248:261-9. 1997..In this review, mechanistic aspects of GPCR* phosphorylation related to the distinct properties, regulation and modes of action of GRKs are described... - Ca(2+)-binding proteins in the retina: from discovery to etiology of human disease(1)I Sokal
Department of Ophthalmology, University of Washington, Seattle, WA 98195 6485, USA
Biochim Biophys Acta 1498:233-51. 2000..These results suggest that CaM-like CaBPs have evolved into diverse subfamilies that control fundamental processes in cells where they are expressed... - Functional reconstitution of photoreceptor guanylate cyclase with native and mutant forms of guanylate cyclase-activating protein 1A Otto Bruc
Department of Ophthalmology, School of Medicine, University of Washington, Seattle 98195, USA
Biochemistry 36:4295-302. 1997..All mutants bound to ROS membranes in a Ca2+-independent manner, except delta25-GCAP1, which was mostly soluble. These findings suggest that the N-terminal region is important in tethering of GCAP1 to the ROS membranes... - Characterization of a dehydrogenase activity responsible for oxidation of 11-cis-retinol in the retinal pigment epithelium of mice with a disrupted RDH5 gene. A model for the human hereditary disease fundus albipunctatusG F Jang
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 276:32456-65. 2001..Furthermore, our results provide detailed characterization of a mouse model for the human disease fundus albipunctatus and emphasize the importance of 11-cis-RDH in keeping the balance between different components of the retinoid cycle... - Calcium-sensitive regions of GCAP1 as observed by chemical modifications, fluorescence, and EPR spectroscopiesI Sokal
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 276:43361-73. 2001.... - Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentrationI Sokal
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 274:19829-37. 1999..Thus, GCAP1 displays thermodynamic and kinetic properties that are compatible with its involvement early in the phototransduction response... - Molecular cloning and localization of rhodopsin kinase in the mammalian pinealX Zhao
Department of Ophthalmology, University of Washington, Seattle 98195 6485, USA
Vis Neurosci 14:225-32. 1997..These findings correlate with an earlier report that neonatal rats show extraretinal light sensitivity, and suggest that a functional photoreceptive system may be present in the adult mammalian pineal... - Guanylyl cyclase activating protein. A calcium-sensitive regulator of phototransductionW A Gorczyca
Department of Ophthalmology, University of Washington, Seattle 98195
J Biol Chem 270:22029-36. 1995.... - Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulinF Haeseleer
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 275:1247-60. 2000..These data suggest that these novel CaBPs are an important component of Ca(2+)-mediated cellular signal transduction in the central nervous system where they may augment or substitute for CaM... - GCAP1 (Y99C) mutant is constitutively active in autosomal dominant cone dystrophyI Sokal
Department of Ophthalmology, School of Medicine, University of Washington, Seattle 98195, USA
Mol Cell 2:129-33. 1998..Alterations in physiological cGMP levels are also associated with other retinal degenerations, including Leber's congenital amaurosis... - Short-chain dehydrogenases/reductases in retinaF Haeseleer
Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195-6485, USA
Methods Enzymol 316:372-83. 2000 - Rod and cone visual cycle consequences of a null mutation in the 11-cis-retinol dehydrogenase gene in manA V Cideciyan
Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia 19104, USA
Vis Neurosci 17:667-78. 2000..Pathways in addition to 11-cis-RDH likely provide 11-cis-retinal for rods and cones and can maintain normal kinetics of visual recovery but only under certain constraints and less efficiently for cone than rod function... - Cloning and characterization of a human beta,beta-carotene-15,15'-dioxygenase that is highly expressed in the retinal pigment epitheliumW Yan
Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21287, USA
Genomics 72:193-202. 2001..In addition, the finding that many of the enzymes involved in retinoid metabolism are mutated in retinal degenerations suggests that BCDO may also be a candidate gene for retinal degenerative disease... - Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamilyF Haeseleer
Department of Ophthalmology, University of Washington School of Medicine, Seattle, Washington 98195, USA
J Biol Chem 274:6526-35. 1999..1, suggesting an ancestral gene duplication/translocation event. The identification of multiple Ca2+-binding proteins that interact with GC is suggestive of complex regulatory mechanisms for photoreceptor GC... - A novel form of rhodopsin kinase from chicken retina and pineal glandX Zhao
Department of Ophthalmology, University of Washington, Seattle 98195, USA
FEBS Lett 454:115-21. 1999..Based on results of molecular cloning and immunolocalization, it appears that both rod and cone photoreceptors express this novel GRK1. These data indicate a larger sequence diversity of photoreceptor GRKs than anticipated previously... - Structural and enzymatic aspects of rhodopsin phosphorylationH Ohguro
Department of Ophthalmology, School of Medicine, University of Washington, Seattle, 98195, USA
J Biol Chem 271:5215-24. 1996..These data provide a molecular mechanism for the site-specific phosphorylation of Rho observed in vivo... - Identification of a guanylyl cyclase-activating protein-binding site within the catalytic domain of retinal guanylyl cyclase 1I Sokal
Department of Ophthalmology, University of Washington, Seattle 98195, USA
Biochemistry 38:1387-93. 1999..Thus, despite interacting with different activating proteins, both AC and GC activity may be modulated through their respective regions within catalytic domains... - Activation of rhodopsin: new insights from structural and biochemical studiesT Okada
Dept of Ophthalmology, University of Washington, Seattle, WA 98195, USA
Trends Biochem Sci 26:318-24. 2001..In many aspects, rhodopsin might provide a structural and functional template for other members of the GPCR family... - Reduction of all-trans-retinal limits regeneration of visual pigment in miceJ C Saari
Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195 6485, USA
Vision Res 38:1325-33. 1998.... - Crystal structure of rhodopsin: a template for cone visual pigments and other G protein-coupled receptorsR E Stenkamp
Department of Biological Structure, University of Washington, Seattle, WA 98195, USA
Biochim Biophys Acta 1565:168-82. 2002..Homology models of the cone pigments provide a means of understanding the roles of amino acid sequence differences that shift the absorption maximum of the retinal chromophore in the environments of different opsins... - Molecular forms of human rhodopsin kinase (GRK1)X Zhao
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 273:5124-31. 1998..Thus, these studies suggest that rods and cones, express the same form of GRK1... - Mechanism of rhodopsin activation as examined with ring-constrained retinal analogs and the crystal structure of the ground state proteinG F Jang
Department of Ophthalmology, University of Washington, Seattle, Washington 98195
J Biol Chem 276:26148-53. 2001..These results suggest that the native chromophore cis-trans isomerization is merely a mechanism for repositioning of the beta-ionone ring which ultimately leads to helix movements and determines receptor activation... - Confronting complexity: the interlink of phototransduction and retinoid metabolism in the vertebrate retinaJ K McBee
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
Prog Retin Eye Res 20:469-529. 2001..The aim of this review is to summarize recent developments in our current understanding of the retinoid cycle at the molecular level, and to examine the relevance of these reactions to phototransduction... - Localization of guanylate cyclase-activating protein 2 in mammalian retinasA Otto-Bruc
Department of Ophthalmology, School of Medicine, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 94:4727-32. 1997..These results suggest that although GCAP1 is involved in the Ca2+-sensitive regulation of GC in rod and cone outer segments, GCAP2 may have non-phototransduction functions in photoreceptors and inner retinal neurons... - Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxinA E Otto Bruc
Department of Ophthalmology, University of Washington, Seattle, WA 98195 6485, USA
Proc Natl Acad Sci U S A 95:15014-9. 1998..These results suggest that under physiological conditions, Rho* phosphorylation is insensitive to regulation by Ca2+ and Ca2+-binding proteins, including recoverin... - Rapid restoration of visual pigment and function with oral retinoid in a mouse model of childhood blindnessJ P Van Hooser
Departments of Ophthalmology, Chemistry, and Pharmacology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 97:8623-8. 2000.... - Isomerization of 11-cis-retinoids to all-trans-retinoids in vitro and in vivoJ K McBee
Departments of Ophthalmology, Pharmacology, and Chemistry, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 276:48483-93. 2001..This study demonstrates an uncharacterized activity of RPE microsomes that could be important in the normal flow of retinoids in the eye in vivo during dark adaptation... - Molecular characterization of a novel short-chain dehydrogenase/reductase that reduces all-trans-retinalF Haeseleer
Department of Ophthalmology, University of Washington School of Medicine, Seattle, Washington 98195 6485, USA
J Biol Chem 273:21790-9. 1998..The presence of retSDR1 in a subset of inner retinal neurons and in other tissues suggests that the enzyme may also be involved in retinol metabolism outside of photoreceptors... - Null mutation in the rhodopsin kinase gene slows recovery kinetics of rod and cone phototransduction in manA V Cideciyan
Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 95:328-33. 1998..Cones may rely mainly on regeneration for the inactivation of photolyzed visual pigment, but RK also contributes to cone recovery... - Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase-activating proteinK Palczewski
Department of Ophthalmology, University of Washington, Seattle 98195
Neuron 13:395-404. 1994.... - Gene transfer mediated by recombinant baculovirus into mouse eyeF Haeseleer
Department of Ophthalmology, University of Washington, 1959 NE Pacific Street, Box 356 485, Seattle, WA 98195 6485, USA
Invest Ophthalmol Vis Sci 42:3294-300. 2001..To determine the efficiency of baculoviruses (BVs) to transfer recombinant genes in vivo into murine ocular tissues... - Crystal structure of rhodopsin: a G-protein-coupled receptorR E Stenkamp
Department of Biological Structure, University of Washington Seattle, WA 98195-7420, USA
Chembiochem 3:963-7. 2002 - Alligator rhodopsin: sequence and biochemical propertiesW C Smith
Department of Ophthalmology, University of Florida, Gainesville 32610, USA
Exp Eye Res 61:569-78. 1995..Phylogenetic analysis of 17 rhodopsin sequences indicates that the alligator is more closely related to the chicken than to the other species examined... - A critical role of CaBP4 in the cone synapseTadao Maeda
Department of Ophthalmology, University of Washington, Seattle, 98195, USA
Invest Ophthalmol Vis Sci 46:4320-7. 2005..In this study, double CaBP4/rod alpha-transducin knockout (Cabp4(-/-)Gnat1(-/-)) mice lacking the rod-mediated component of electrophysiologic responses were generated and analyzed to investigate the role of CaBP4 in cones... - Retinoid absorption and storage is impaired in mice lacking lecithin:retinol acyltransferase (LRAT)Sheila M O'Byrne
Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
J Biol Chem 280:35647-57. 2005..The fatty acyl composition of these (chylomicron) retinyl esters suggests that they are synthesized via an acyl-CoA-dependent process suggesting the existence of a physiologically significant acyl-CoA:retinol acyltransferase... - Partial agonism in a G Protein-coupled receptor: role of the retinal ring structure in rhodopsin activationFranz J Bartl
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Schumannstrasse 20 21, D 10098 Berlin, Germany
J Biol Chem 280:34259-67. 2005..We conclude that the main role of the ring structure is to maintain the active state, thus specifying a mechanism of activation by a partial agonist of the G protein-coupled receptor rhodopsin... - Detecting molecular interactions that stabilize native bovine rhodopsinK Tanuj Sapra
Center for Biotechnology, University of Technology, 01307 Dresden, Germany
J Mol Biol 358:255-69. 2006..These changes highlight the structural importance of this disulfide bond and may form the basis of dysfunctions associated with its absence... - Structural analysis of Mg2+ and Ca2+ binding to CaBP1, a neuron-specific regulator of calcium channelsJennifer N Wingard
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, 20850, USA
J Biol Chem 280:37461-70. 2005..Both conformational states may serve to modulate the activity of Ca(2+) channel targets... - Metabolism and transactivation activity of 13,14-dihydroretinoic acidAlexander R Moise
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 280:27815-25. 2005..This new metabolite can also be an intermediate in a retinol degradation pathway or it can serve as a precursor for the synthesis of bioactive 13,14-dihydroretinoid metabolites... - Related enzymes solve evolutionarily recurrent problems in the metabolism of carotenoidsAlexander R Moise
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
Trends Plant Sci 10:178-86. 2005.... - The supramolecular structure of the GPCR rhodopsin in solution and native disc membranesKitaru Suda
ME Muller Institute for Microscopy, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
Mol Membr Biol 21:435-46. 2004..These results corroborate the recent findings from atomic force microscopy and molecular modeling on the supramolecular structure and packing arrangement of murine rhodopsin dimers... - Role of photoreceptor-specific retinol dehydrogenase in the retinoid cycle in vivoAkiko Maeda
Department of Ophthalmology, University of Washington, Seattle, Washington 98195 6485, USA
J Biol Chem 280:18822-32. 2005..We conclude that prRDH is an enzyme that catalyzes reduction of all-trans-retinal in the rod outer segment, most noticeably at higher light intensities and prolonged illumination, but is not an essential enzyme of the retinoid cycle... - Oligomerization of G protein-coupled receptors: past, present, and futurePaul S H Park
Department of Ophthalmology, University of Washington, Seattle, Washington 98195 6485, USA
Biochemistry 43:15643-56. 2004..Cooperative interactions within such an oligomeric array may be critical for the propagation of an external signal across the cell membrane and to the G protein, and may therefore underlie the mechanistic basis of signaling... - A novel mutation (I143NT) in guanylate cyclase-activating protein 1 (GCAP1) associated with autosomal dominant cone degenerationKoji M Nishiguchi
Ocular Molecular Genetics Institute, Massachusetts Eye and Ear Infirmary, Boston, MA, USA
Invest Ophthalmol Vis Sci 45:3863-70. 2004..To identify pathogenic mutations in the guanylate cyclase-activating protein 1 (GCAP1) and GCAP2 genes and to characterize the biochemical effect of mutation on guanylate cyclase (GC) stimulation... - Structure of the rhodopsin dimer: a working model for G-protein-coupled receptorsDimitrios Fotiadis
, Biozentrum, University of Basel, CH-4056 Basel, Switzerland
Curr Opin Struct Biol 16:252-9. 2006..Recent functional analyses of fractions from solubilized disk membranes revealed that higher-order Rho oligomers are the most active species. These and other results have enhanced our understanding of GPCR structure and function... - Inner retinal photoreception independent of the visual retinoid cycleDaniel C Tu
Department of Ophthalmology and Visual Sciences, Washington University Medical School, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 103:10426-31. 2006..These results demonstrate that the melanopsin-dependent ipRGC photocycle is independent of the visual retinoid cycle... - Improvements in G protein-coupled receptor purification yield light stable rhodopsin crystalsDavid Salom
Novasite Pharmaceuticals, Inc, San Diego, CA 92121, USA
J Struct Biol 156:497-504. 2006..Importantly, these new crystals are stable upon photoactivation and the preliminary X-ray diffraction analysis of both photoactivated and ground state rhodopsin crystals are also reported... - Impact of retinal disease-associated RPE65 mutations on retinoid isomerizationGrzegorz Bereta
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
Biochemistry 47:9856-65. 2008.... - Trafficking of membrane-associated proteins to cone photoreceptor outer segments requires the chromophore 11-cis-retinalHoubin Zhang
Department of Ophthalmology, University of Utah Health Science Center, Salt Lake City, Utah 84132, USA
J Neurosci 28:4008-14. 2008..Furthermore, the presence of 11-cis-retinal is essential for proper transport of several membrane-associated cone phototransduction polypeptides in these cones... - Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activationPuja Singh
Life Sciences Institute, Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
J Biol Chem 283:14053-62. 2008..Co-localization of the N terminus and the C-terminal extension near the hinge of the kinase domain suggests that activated GPCRs stimulate kinase activity by binding to this region to facilitate full closure of the kinase domain... - Mechanical properties of bovine rhodopsin and bacteriorhodopsin: possible roles in folding and functionK Tanuj Sapra
Biotechnology Center, University of Technology, Dresden, Germany
Langmuir 24:1330-7. 2008..This core may reflect differences in mechanisms of protein folding between the two membrane proteins. The different structural rigidity of the two proteins may also reflect their adaptation to differing functions... - Metabolic basis of visual cycle inhibition by retinoid and nonretinoid compounds in the vertebrate retinaMarcin Golczak
Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
J Biol Chem 283:9543-54. 2008..Finally, we provide evidence for a crucial role of lecithin: retinol acyltransferase activity in mediating tissue specific absorption and long lasting therapeutic effects of retinoid-based visual cycle inhibitors... - Stereospecificity of retinol saturase: absolute configuration, synthesis, and biological evaluation of dihydroretinoidsAlexander R Moise
Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4965, USA
J Am Chem Soc 130:1154-5. 2008 - Redundant and unique roles of retinol dehydrogenases in the mouse retinaAkiko Maeda
Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
Proc Natl Acad Sci U S A 104:19565-70. 2007..The results suggest that RDH8 and RDH12 are dispensable in support of the visual cycle but appear to be key components in clearance of free all-trans-retinal, thereby preventing A2E accumulation and photoreceptor cell death... - Crystal packing analysis of Rhodopsin crystalsDavid T Lodowski
Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA
J Struct Biol 158:455-62. 2007..The inter-monomer surface contacts are different for each model, but several of these models coincide in implicating helix I, II, and H-8 as contributors to the main contact surface stabilizing the dimers... - Stabilizing effect of Zn2+ in native bovine rhodopsinPaul S H Park
Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Biol Chem 282:11377-85. 2007..The inclusion of ZnCl2 in SMFS assay buffer increased the stability of most structural segments. This effect was not mimicked by CaCl2, CdCl2, or CoCl2. Thus, Zn2+ stabilizes the structure of rhodopsin in a specific manner... - Crystal structure of a photoactivated deprotonated intermediate of rhodopsinDavid Salom
Novasite Pharmaceuticals Inc, San Diego, CA 92121, USA
Proc Natl Acad Sci U S A 103:16123-8. 2006..The activation and oligomerization models likely extend to the majority of other G protein-coupled receptors... - Improvement in rod and cone function in mouse model of Fundus albipunctatus after pharmacologic treatment with 9-cis-retinalAkiko Maeda
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
Invest Ophthalmol Vis Sci 47:4540-6. 2006..To assess changes in rod and cone visual functions in a mouse model of Fundus albipunctatus with disrupted 11-cis-retinol dehydrogenase (RDH) genes after pharmacologic treatment with an artificial retinal chromophore... - Diseases caused by defects in the visual cycle: retinoids as potential therapeutic agentsGabriel H Travis
Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA 90095, USA
Annu Rev Pharmacol Toxicol 47:469-512. 2007..Recent progress in both areas provides hope that multiple inherited retinal diseases will soon be treated by pharmaceutical intervention... - Light-driven cone arrestin translocation in cones of postnatal guanylate cyclase-1 knockout mouse retina treated with AAV-GC1Shannon E Haire
Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610 0244, USA
Invest Ophthalmol Vis Sci 47:3745-53. 2006..The purpose of this study was to determine whether the expression of GC1 restores cone arrestin translocation in the cone cells of postnatal GC1 knockout mouse retina... - Functional characterization of rhodopsin monomers and dimers in detergentsBeata Jastrzebska
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:54663-75. 2004..These studies provide an explanation of how detergent influences the oligomer-dimermonomer equilibrium of Rho and describe the functional characterization of Rho monomers and dimers in detergent... - Diversity of guanylate cyclase-activating proteins (GCAPs) in teleost fish: characterization of three novel GCAPs (GCAP4, GCAP5, GCAP7) from zebrafish (Danio rerio) and prediction of eight GCAPs (GCAP1-8) in pufferfish (Fugu rubripes)Yoshikazu Imanishi
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
J Mol Evol 59:204-17. 2004..Based on genome searches and EST analyses, the mouse and human genomes do not harbor GCAP4-8 or GCIP genes... - A naturally occurring mutation of the opsin gene (T4R) in dogs affects glycosylation and stability of the G protein-coupled receptorLi Zhu
Department of Ophthalmology, University of Washington, 1957 NE Pacific St, Seattle, WA 98195-6485, USA
J Biol Chem 279:53828-39. 2004..The T4R mutation may represent a novel molecular mechanism of degeneration where the unliganded form of the mutant opsin exerts a detrimental effect by losing its structural integrity... - Role of the conserved NPxxY(x)5,6F motif in the rhodopsin ground state and during activationOlaf Fritze
Institut fur Medizinische Physik und Biophysik, Universitatsklinikum Charite, Humboldt Universitat, Schumann Strasse 20 21, D 10098 Berlin, Germany
Proc Natl Acad Sci U S A 100:2290-5. 2003..The dual control of the structural rearrangements secures reliable transformation of quiescent rhodopsin to activating Meta II... - Calmodulin and Ca2+-binding proteins (CaBPs): variations on a themeFrancoise Haeseleer
Department of Ophthalmology, University of Washington, Seattle, Washington 98195 6485, USA
Adv Exp Med Biol 514:303-17. 2002..CaBPs could be cell-type specific proteins that play a key role in the Ca2+ signaling of specialized retinal neurons... - The crystallographic model of rhodopsin and its use in studies of other G protein-coupled receptorsSlawomir Filipek
Department of Biological Structure, University of Washington, Seattle, Washington 98195, USA
Annu Rev Biophys Biomol Struct 32:375-97. 2003..Use of the rhodopsin structural model to understand the structure and function of other GPCRs provides strong evidence validating the structural model... - Pharmacological chaperone-mediated in vivo folding and stabilization of the P23H-opsin mutant associated with autosomal dominant retinitis pigmentosaSyed M Noorwez
Department of Ophthalmology, University of Florida, Gainesville 55455, USA
J Biol Chem 278:14442-50. 2003..These properties suggest that 11-cis-7-ring retinal may be a useful therapeutic agent for the rescue of P23H-opsin and the prevention of retinal degeneration... - Signaling states of rhodopsin. Formation of the storage form, metarhodopsin III, from active metarhodopsin IIMartin Heck
Institut fur Medizinische Physik und Biophysik, Universitatsklinikum Charite, Humboldt Universitat zu Berlin, Schumannstrasse 20 21, 10098 Berlin, Germany
J Biol Chem 278:3162-9. 2003..Therefore, Meta III could be a form of rhodopsin that is stored away, thus regulating photoreceptor regeneration... - Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retinaFrancoise Haeseleer
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 277:45537-46. 2002..These newly identified enzymes add new elements to important retinoid metabolic pathways that have not been explained by previous genetic and biochemical studies... - Vaccination with recoverin, a cancer-associated retinopathy antigen, induces autoimmune retinal dysfunction and tumor cell regression in miceAkiko Maeda
Department of Ophthalmology, University of Washington, Seattle, WA 98195-6485, USA
Eur J Immunol 32:2300-7. 2002.... - Identification of a family of calcium sensors as protein ligands of inositol trisphosphate receptor Ca(2+) release channelsJun Yang
Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104 6085, USA
Proc Natl Acad Sci U S A 99:7711-6. 2002.... - GCAP1 rescues rod photoreceptor response in GCAP1/GCAP2 knockout miceKim A Howes
Department of Ophthalmology, Moran Eye Center, University of Utah Health Science Center, Salt Lake City, UT 84112 5330, USA
EMBO J 21:1545-54. 2002..These results demonstrate that GCAP1 at near normal levels supports the generation of wild-type flash responses in the absence of GCAP2... - Differential modulation of Ca(v)2.1 channels by calmodulin and Ca2+-binding protein 1Amy Lee
Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington 98195 7280, USA
Nat Neurosci 5:210-7. 2002..Our results identify an interaction between Ca2+ channels and CaBP1 that may regulate Ca2+-dependent forms of synaptic plasticity by inhibiting Ca2+ influx into neurons... - Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to manYoshikazu Imanishi
Department of Ophthalmology, Pharmacology, and Chemistry, University of Washington, Seattle, WA 98195, USA
Eur J Neurosci 15:63-78. 2002.... - Calcium-binding proteins: intracellular sensors from the calmodulin superfamilyFrancoise Haeseleer
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
Biochem Biophys Res Commun 290:615-23. 2002..A large number of NCBP and CaBP subfamily members would benefit from their potentially specialized role in Ca(2+)-dependent cellular processes. Pinpointing the role of these proteins will be a challenging task for further research... - Organization of the G protein-coupled receptors rhodopsin and opsin in native membranesYan Liang
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
J Biol Chem 278:21655-62. 2003..This is the first semi-empirical model of a higher order structure of a GPCR in native membranes, and it has profound implications for the understanding of how this receptor interacts with partner proteins... - Ligand channeling within a G-protein-coupled receptor. The entry and exit of retinals in native opsinSandra A Schadel
, , , Schumannstrasse 20-21, Germany
J Biol Chem 278:24896-903. 2003..This mechanism enables a unidirectional process for the release of photoisomerized chromophore and the uptake of newly synthesized 11-cis-retinal for the regeneration of rhodopsin... - Evaluation of the role of the retinal G protein-coupled receptor (RGR) in the vertebrate retina in vivoTadao Maeda
Department of Ophthalmology, University of Washington, Seattle, WA 98195 6485, USA
J Neurochem 85:944-56. 2003.... - Essential role of Ca2+-binding protein 4, a Cav1.4 channel regulator, in photoreceptor synaptic functionFrancoise Haeseleer
Department of Ophthalmology, University of Washington, Box 356485, Seattle, Washington 98195, USA
Nat Neurosci 7:1079-87. 2004..These observations indicate that CaBP4 is important for normal synaptic function, probably through regulation of Ca(2+) influx and neurotransmitter release in photoreceptor synaptic terminals... - Ca2+ binding to EF hands 1 and 3 is essential for the interaction of apoptosis-linked gene-2 with Alix/AIP1 in ocular melanomaLalita Subramanian
Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53792, USA
Biochemistry 43:11175-86. 2004..Together, these data indicate that gross surface hydrophobicity changes are insufficient for target recognition... - Identification of all-trans-retinol:all-trans-13,14-dihydroretinol saturaseAlexander R Moise
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:50230-42. 2004..Thus, saturation of all-trans-retinol to all-trans-13,14-dihydroretinol by RetSat produces a new metabolite of yet unknown biological function... - Rhodopsin signaling and organization in heterozygote rhodopsin knockout miceYan Liang
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:48189-96. 2004..From these data, we conclude that the differences in physiological responses measured in WT and Rho+/- mice are due to structural changes of the whole ROS and not due to a lower density of Rho... - Impairment of the transient pupillary light reflex in Rpe65(-/-) mice and humans with leber congenital amaurosisTomas S Aleman
Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Invest Ophthalmol Vis Sci 45:1259-71. 2004..To determine the impairment of the transient pupillary light reflex (TPLR) due to severe retinal dysfunction and degeneration in a murine model of Leber congenital amaurosis (LCA) and in patients with the disease... - Noninvasive two-photon imaging reveals retinyl ester storage structures in the eyeYoshikazu Imanishi
Department of Ophthalmology, University of Washington, 1959 NE Pacific St, Box 356485, Seattle, WA 98195-6485, USA
J Cell Biol 164:373-83. 2004..These results indicate that RESTs located close to the RPE plasma membrane are essential components in 11-cis-retinal production... - Photoreceptor guanylate cyclase variants: cGMP production under controlIzabela Sokal
Department of Ophthalmology, University of Washington, Seattle, WA 98195, U.S.A
Acta Biochim Pol 50:1075-95. 2003..Recent efforts have been directed toward understanding the properties of GC at the physiological and structural levels. Here, we summarize the recent progress and present a list of topics of ongoing research... - Lecithin-retinol acyltransferase is essential for accumulation of all-trans-retinyl esters in the eye and in the liverMatthew L Batten
Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:10422-32. 2004..We conclude that Lrat-/- mice may serve as an animal model with early onset severe retinal dystrophy and severe retinyl ester deprivation... - Evolutionary analysis of rhodopsin and cone pigments: connecting the three-dimensional structure with spectral tuning and signal transferDavid C Teller
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
FEBS Lett 555:151-9. 2003.... - Novel targeting strategy for generating mouse models with defects in the retinoid cycleCarola Driessen
Department of Biochemistry 160, University of Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Vision Res 43:3075-9. 2003..Generation of double and triple knockouts will aid in determining if these retinol dehydrogenases are responsible for the remaining 11-cis-retinol oxidation observed in RDH5 knockout animals... - Retinoid cycle in the vertebrate retina: experimental approaches and mechanisms of isomerizationVladimir Kuksa
Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195, USA
Vision Res 43:2959-81. 2003..The chemistry of the all-trans-retinol to 11-cis-retinol isomerization is also discussed... - Phototransduction: crystal clearKevin D Ridge
Center for Advanced Research in Biotechnology, National Institute of Standards and Technology and the University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
Trends Biochem Sci 28:479-87. 2003..Given the accelerated pace of structure determination, it is anticipated that this will be the first G-protein-coupled pathway for which a complete molecular description is ultimately available... - Soluble fusion proteins between single transmembrane photoreceptor guanylyl cyclases and their activatorsIzabela Sokal
Department of Ophthalmology, University of Washington, Seattle, Washington 98195-6485, USA
Biochemistry 41:251-7. 2002..Thus, fusion proteins may provide an important advance for further structural studies of photoreceptor GCs and other sTMRs with and without different forms of regulatory proteins...