Research Topics
Genomes and Genes | A M PajorSummaryAffiliation: University of Texas Medical Branch Country: USA Publications
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Publications
Molecular properties of the SLC13 family of dicarboxylate and sulfate transportersAna M Pajor
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
Pflugers Arch 451:597-605. 2006..The purpose of this review is to provide an update on new advances in this gene family, particularly on structure-function studies and new members of the family...- Conformationally sensitive residues in extracellular loop 5 of the Na+/dicarboxylate co-transporterAna M Pajor
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas 77555 0645, USA
J Biol Chem 280:18728-35. 2005..Conformational changes in the protein affect the accessibility of the residues in extracellular loop 5 and provide further evidence of large-scale changes in the structure of NaDC-1 during the transport cycle... - Inhibition of the Na+/dicarboxylate cotransporter by anthranilic acid derivativesAna M Pajor
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 0645, USA
Mol Pharmacol 72:1330-6. 2007..In conclusion, ACA and ONO-RS-082 represent promising lead compounds for the development of specific inhibitors of the Na(+)/dicarboxylate cotransporters... - Inhibitor binding in the human renal low- and high-affinity Na+/glucose cotransportersAna M Pajor
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
J Pharmacol Exp Ther 324:985-91. 2008..The differences in the effects of the mutations between hSGLT1 and hSGLT2 suggest that this cysteine holds key residues in place rather than participating directly in inhibitor binding... - Conformationally sensitive residues in transmembrane domain 9 of the Na+/dicarboxylate co-transporterA M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555, USA
J Biol Chem 276:29961-8. 2001..In conclusion, TMD-9 may be involved in transducing conformational changes between the cation-binding sites and the substrate-binding site in NaDC-1, and it may also form part of the translocation pathway through the transporter... - Cloning and functional characterization of a high-affinity Na(+)/dicarboxylate cotransporter from mouse brainA M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555, USA
Am J Physiol Cell Physiol 280:C1215-23. 2001..In conclusion, mNaDC-3 likely codes for the high-affinity Na(+)/dicarboxylate cotransporter in brain, and it has some unusual electrical properties compared with the other members of the family... - Role of cationic amino acids in the Na+/dicarboxylate co-transporter NaDC-1A M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555 0641, USA
Biochem J 350:677-83. 2000..In conclusion, charge neutralization of Lys-84 and Arg-349 in NaDC-1 affects succinate handling, suggesting that these residues might have roles in substrate binding... - Molecular cloning, chromosomal organization, and functional characterization of a sodium-dicarboxylate cotransporter from mouse kidneyA M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555, USA
Am J Physiol Renal Physiol 279:F482-90. 2000..However, its function more closely resembles the rabbit and human orthologs rather than the rat NaDC-1, with which it shares higher sequence similarity... - Cysteine residues in the Na+/dicarboxylate co-transporter, NaDC-1A M Pajor
Department of Physiology, University of Texas Medical Branch, Galveston, TX 77555 0641, USA
Biochem J 344:205-9. 1999..The results indicate that mutagenesis of multiple cysteine residues in NaDC-1 may alter the shape or configuration of the protein, leading to alterations in protein trafficking or stability... - Protein kinase C-mediated regulation of the renal Na(+)/dicarboxylate cotransporter, NaDC-1A M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555 0641, USA
Biochim Biophys Acta 1420:223-30. 1999..We conclude that the inhibition of NaDC-1 transport activity by PMA occurs by a combination of endocytosis and inhibition of transport activity... - Sodium-coupled transporters for Krebs cycle intermediatesA M Pajor
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, USA
Annu Rev Physiol 61:663-82. 1999..The current model of secondary structure in NaDC-1 contains 11 transmembrane domains and an extracellular N-glycosylated carboxy terminus... - The substrate recognition domain in the Na+/dicarboxylate and Na+/sulfate cotransporters is located in the carboxy-terminal portion of the proteinA M Pajor
Department of Physiology, University of Arizona, College of Medicine, Tucson, AZ 85724, USA
Biochim Biophys Acta 1370:98-106. 1998.... - Water transport by the renal Na(+)-dicarboxylate cotransporterA K Meinild
Department of Physiology, University of California, School of Medicine, Los Angeles, California 90095 1751, USA
Am J Physiol Renal Physiol 278:F777-83. 2000..These results indicate that the renal Na(+)-dicarboxylate cotransporter mediates water transport and, under physiological conditions, may contribute to fluid reabsorption across the proximal tubule... - Threonine-509 is a determinant of apparent affinity for both substrate and cations in the human Na+/dicarboxylate cotransporterJittima Weerachayaphorn
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 0645, USA
Biochemistry 47:1087-93. 2008..Furthermore, the results suggest that the cation and substrate binding sites are located in close proximity to one another in NaDC1... - Expression cloning of NaDC-2, an intestinal Na(+)- or Li(+)-dependent dicarboxylate transporterL Bai
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724 5051, USA
Am J Physiol 273:G267-74. 1997..The differences in functional properties between NaDC-2 and the structurally related Na(+)-dicarboxylate cotransporters NaDC-1 and hNaDC-1 will form the basis of detailed structure-function studies... - Molecular cloning and functional expression of a sodium-dicarboxylate cotransporter from human kidneyA M Pajor
University of Arizona, Department of Physiology, College of Medicine, Tucson 85724, USA
Am J Physiol 270:F642-8. 1996.... - Arginine-349 and aspartate-373 of the Na(+)/dicarboxylate cotransporter are conformationally sensitive residuesXiaozhou Yao
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555-0641, USA
Biochemistry 41:1083-90. 2002..We conclude that the accessibility of Arg-349 and Asp-373 is likely to change with the conformational states of the transport cycle... - Primary structure and functional characteristics of a mammalian sodium-coupled high affinity dicarboxylate transporterR Kekuda
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia 30912, USA
J Biol Chem 274:3422-9. 1999..This represents the first report on the cloning and functional characterization of a mammalian Na+-dependent, high affinity dicarboxylate transporter... - Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularlyF F Zhang
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555-0641, USA
Biochim Biophys Acta 1511:80-9. 2001..The results of this study show that both the N-terminus and hydrophilic loop 4 of NaDC-1 are located intracellularly, which supports the current model of NaDC-1 structure... - Sodium-dependent extracellular accessibility of Lys-84 in the sodium/dicarboxylate cotransporterJittima Weerachayaphorn
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 0645, USA
J Biol Chem 282:20213-20. 2007..The results point to a new location for Lys-84 within the substrate access pore of the Na(+)/dicarboxylate cotransporter, either in a transmembrane helix or a reentrant loop facing a water-filled pore... - Functional reconstitution of SdcS, a Na+-coupled dicarboxylate carrier protein from Staphylococcus aureusJason A Hall
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 0645, USA
J Bacteriol 189:880-5. 2007.... - Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2Jittima Weerachayaphorn
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 0645, USA
Biochim Biophys Acta 1778:1051-9. 2008..Since many of these pathways are not found in human intestine, this model may be best suited for studying Na+-dependent transport of succinate by NaDC1... - Functional characterization of CitM, the Mg2+-citrate transporterH Li
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555 0641, USA
J Membr Biol 185:9-16. 2002..coli has properties similar to the transporter in intact B. subtilis cells. The results support a transport model with a coupling stoichiometry of one proton coupled to the uptake of one complex of (Mg2+-citrate)1-... - Sequence of a pyrimidine-selective Na+/nucleoside cotransporter from pig kidney, pkCNT1A M Pajor
University of Arizona, Department of Physiology, College of Medicine, Tucson, AZ 85724 5051
Biochim Biophys Acta 1415:266-9. 1998..pkCNT1 transports pyrimidines, such as thymidine and uridine, and has a Km for uridine of 9 microM... - Functional characterization of a Na(+)-coupled dicarboxylate carrier protein from Staphylococcus aureusJason A Hall
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 0647, USA
J Bacteriol 187:5189-94. 2005..5) of 2.7 mM) binding before dicarboxylate (K(m) of 4.5 microM). Because this transporter and its mammalian homologs are functionally similar, we suggest that SdcS may serve as a useful model for DASS family structural analysis... - Serines 260 and 288 are involved in sulfate transport by hNaSi-1Hongyan Li
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555 0641, USA
J Biol Chem 278:37204-12. 2003..Because the four residues are located in transmembrane domain 5, this transmembrane domain is likely to participate in the conformational movements during the transport cycle of hNaSi-1... - Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1Naomi Oshiro
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 0645, USA
Biochemistry 45:2302-10. 2006..We conclude that TM 3 and 4 contain amino acid residues that are important determinants of substrate specificity and catalytic efficiency in NaDC1... - Functional characterization of high-affinity Na(+)/dicarboxylate cotransporter found in Xenopus laevis kidney and heartNaomi Oshiro
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 0645, USA
Am J Physiol Cell Physiol 289:C1159-68. 2005..However, other electrophysiological properties of xNaDC-3 are unique and involve large leak currents, possibly mediated by anions, that are activated by binding of sodium or lithium to a single site... - Mutagenesis of the N-glycosylation site of hNaSi-1 reduces transport activityHongyan Li
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555 0641, USA
Am J Physiol Cell Physiol 285:C1188-96. 2003..These results suggest that Asn591 and/or N-glycosylation is critical for transport activity in NaSi-1... - Role of conserved prolines in the structure and function of the Na+/dicarboxylate cotransporter 1, NaDC1Aditya D Joshi
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-0645, USA
Biochemistry 45:4231-9. 2006..The prolines in TM 10, at positions 523 and 524, may not be directly involved in the transporter function but may be necessary for maintaining structure... - Ala-504 is a determinant of substrate binding affinity in the mouse Na(+)/dicarboxylate cotransporterNaomi Oshiro
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 0645, USA
Biochim Biophys Acta 1758:781-8. 2006..We conclude that multiple amino acids from TM 8, 9 and 10 contribute to the transport of adipate in NaDC1. Furthermore, Ala-504 in TM 10 is an important determinant of K(0.5) for both adipate and succinate... - OKP cells express the Na-dicarboxylate cotransporter NaDC-1Seiji Aruga
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-8856, USA
Am J Physiol Cell Physiol 287:C64-72. 2004..In OKP cells transfected with a green fluorescent protein-oNaDC-1 construct, however, media acidification increases Na-dependent citrate uptake, demonstrating posttranscriptional acid regulation of NaDC-1 activity...
Research Grants
- Involvement of Na/Dicarboxylate Cotransporter in AgingAna Pajor; Fiscal Year: 2002..abstract_text> ..