Research Topics
| G P OwensSummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
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Detail Information
Publications
VH4 gene segments dominate the intrathecal humoral immune response in multiple sclerosisGregory P Owens
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
J Immunol 179:6343-51. 2007..These results indicate a highly restricted B cell response in MS. Identifying the targets of CeSF plasma cells may yield insights into disease pathogenesis...- Measles virus-specific plasma cells are prominent in subacute sclerosing panencephalitis CSFG P Owens
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Neurology 68:1815-9. 2007..To demonstrate the specificity of expanded CD138(+) plasma cell clones recovered from the CSF of a patient with subacute sclerosing panencephalitis (SSPE) for measles virus (MV)... - Single-cell repertoire analysis demonstrates that clonal expansion is a prominent feature of the B cell response in multiple sclerosis cerebrospinal fluidGregory P Owens
Department of Neurology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Mail Stop Box B182, Denver, CO 80262, USA
J Immunol 171:2725-33. 2003..The most immediate implications of identifying rearranged Ig sequences in MS B cells is the potential to accurately recreate recombinant Abs from these overrepresented H and L chains that can be used to discover the relevant Ag(s) in MS... - Extraction and purification of active IgG from SSPE and MS brainG P Owens
Department of Neurology, University of Colorado Health Sciences Center, Denver 80262, USA
J Virol Methods 68:119-25. 1997.... - B-lymphocyte and plasma cell clonal expansion in monosymptomatic optic neuritis cerebrospinal fluidKurt Haubold
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Ann Neurol 56:97-107. 2004.... - Absence of Epstein-Barr virus in the brain and CSF of patients with multiple sclerosisS A Sargsyan
Department of Neurology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
Neurology 74:1127-35. 2010..Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that becomes latent in B-lymphocytes and has been implicated in the pathogenesis of multiple sclerosis (MS). We searched for latent and active EBV infection in MS brain and CSF... - Molecular immunologic strategies to identify antigens and b-cell responses unique to multiple sclerosisD H Gilden
Department of Neurology, University of Colorado Health Sciences Center, 4200 E Ninth Ave, Mail Stop B182, Denver, CO 80262, USA
Arch Neurol 58:43-8. 2001..New sophisticated molecular immunologic techniques described herein should enhance our ability to identify putative antigen(s) targets in MS... - The immunoglobulin G heavy chain repertoire in multiple sclerosis plaques is distinct from the heavy chain repertoire in peripheral blood lymphocytesG P Owens
Department of Neurology, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA
Clin Immunol 98:258-63. 2001..None of the VH sequences expressed in plaque regions were detected in peripheral blood lymphocytes from this patient. These data indicate the presence of a CNS-targeted antigen-driven response in MS plaques... - Laser capture microdissection and single-cell RT-PCR without RNA purificationKathryne Melissa Keays
Department of Neurology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Mail Stop B182, Denver, CO 80262, United States
J Immunol Methods 302:90-8. 2005..These techniques will streamline the identification of gene expression products in single cells from complex tissues and have the potential to identify IgGs expressed in the CNS of inflammatory diseases of unknown etiology... - Antigen discovery in chronic human inflammatory central nervous system disease: panning phage-displayed antigen libraries identifies the targets of central nervous system-derived IgG in subacute sclerosing panencephalitisM P Burgoon
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
J Immunol 167:6009-14. 2001..This strategy will be useful to identify potentially rare Ags in diseases of unknown cause... - Screening random peptide libraries with subacute sclerosing panencephalitis brain-derived recombinant antibodies identifies multiple epitopes in the C-terminal region of the measles virus nucleocapsid proteinGregory P Owens
Department of Neurology, University of Colorado Health Sciences Center, 4200 E. 9th Avenue, Mail Stop B182, Denver, CO 80262, USA
J Virol 80:12121-30. 2006..These results indicate the feasibility of using peptide screening for antigen discovery in central nervous system inflammatory diseases of unknown etiology, such as multiple sclerosis, neurosarcoidosis, or Behcet's syndrome... - Antibodies produced by clonally expanded plasma cells in multiple sclerosis cerebrospinal fluidGregory P Owens
Department of Neurology, University of Colorado Denver School of Medicine, Denver, CO 80045, USA
Ann Neurol 65:639-49. 2009..Nevertheless, the target antigen of this response remains enigmatic... - Comparative analysis of the CD19+ and CD138+ cell antibody repertoires in the cerebrospinal fluid of patients with multiple sclerosisAlanna M Ritchie
Department of Neurology, University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262, USA
J Immunol 173:649-56. 2004..Detection of plasma cell clones by single-cell PCR will facilitate the in vitro production of recombinant Abs useful in identifying disease-relevant Ags... - CSF IgG heavy-chain bias in patients at the time of a clinically isolated syndromeJeffrey L Bennett
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO, United States
J Neuroimmunol 199:126-32. 2008..Clinically, subjects with VH4 or VH2 CSF IgG repertoire bias rapidly progressed to definite MS, whereas individuals without repertoire bias did not develop MS after a minimum of 2 years of follow-up (p=0.01)... - Varicella zoster virus is not a disease-relevant antigen in multiple sclerosisMark P Burgoon
Department of Neurology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
Ann Neurol 65:474-9. 2009..VZV is not a disease-relevant antigen in MS... - Construction of recombinant mouse IgG1 antibody directed against varicella zoster virus immediate early protein 63Niklaus H Mueller
Department of Neurology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
Hybridoma (Larchmt) 27:1-10. 2008.... - Potential of a unique antibody gene signature to predict conversion to clinically definite multiple sclerosisElizabeth M Cameron
Department of Neurology, University of Texas Southwestern Medical Center, Dallas TX 75154, USA
J Neuroimmunol 213:123-30. 2009..If confirmed, signature prevalence would be a novel genetic diagnostic tool candidate for patients with early demyelinating disease of the central nervous system... - Specificity of recombinant antibodies generated from multiple sclerosis cerebrospinal fluid probed with a random peptide libraryXiaoli Yu
Department of Neurology, University of Colorado Health Sciences Center, 4200 E 9th Avenue, Mail Stop B182, Denver, Colorado 80262, USA
J Neuroimmunol 172:121-31. 2006..Our data demonstrate that MS rAb reflects the specificity of IgG in the CSF. Thus, the epitopes/mimotopes identified by MS rAb may provide clues to disease-relevant antigens... - Recombinant antibodies generated from both clonal and less abundant plasma cell immunoglobulin G sequences in subacute sclerosing panencephalitis brain are directed against measles virusMark P Burgoon
Department of Neurology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Neurovirol 12:398-402. 2006..The intrathecal response may be useful in determining the causes of other inflammatory CNS diseases, such as multiple sclerosis, Behcet's disease, and neurosarcoidosis... - Rapid and efficient identification of epitopes/mimotopes from random peptide librariesXiaoli Yu
Department of Neurology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Mail Stop B182, Denver, CO 80262, USA
J Immunol Methods 316:67-74. 2006..This technique will expedite identification of MS-specific peptides that can be used to discover the corresponding protein antigens... - Laser-capture microdissection of plasma cells from subacute sclerosing panencephalitis brain reveals intrathecal disease-relevant antibodiesMark P Burgoon
Department of Neurology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262, USA
Proc Natl Acad Sci U S A 102:7245-50. 2005..This strategy can be used to identify disease-relevant antigens in CNS inflammatory diseases of unknown etiology... - Oligoclonal immunoglobulins in cerebrospinal fluid during varicella zoster virus (VZV) vasculopathy are directed against VZVMark P Burgoon
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Ann Neurol 54:459-63. 2003..This approach holds promise in identifying and purifying the relevant oligoclonal IgGs in inflammatory central nervous system diseases of unknown cause... - B cells in multiple sclerosisMark P Burgoon
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Front Biosci 9:786-96. 2004..Identification of these targets will reveal valuable insights into the cause and pathogenesis of MS and is likely to lead to effective treatment... - Proteomic analysis of multiple sclerosis cerebrospinal fluidB N Hammack
Department of Neurology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Mult Scler 10:245-60. 2004..It remains unknown whether these four proteins are related to the cause and pathogenesis of MS... - Improved resolution of human cerebrospinal fluid proteins on two-dimensional gelsB N Hammack
Department of Neurology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Mult Scler 9:472-5. 2003..Improved protein recovery and resolution will facilitate excision from gels for analysis by peptide mass fingerprinting... - The B cell response in multiple sclerosisGregory P Owens
Department of Neurology, University of Colorado Health Sciences Center, Denver, 80262, USA
Neurol Res 28:236-44. 2006..Together with histologic studies showing the presence of ectopic lymphoid follicles in the meninges of some MS patients, this data supports the notion of a targeted and compartmentalized humoral response in MS... - Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blastsKimberly M Winges
Howard Hughes Medical Institute Research Fellow, Chevy Chase, MD 20815, USA
J Neuroimmunol 192:226-34. 2007....