Research Topics
| Tove OlafsenSummaryAffiliation: University of California Country: USA Publications
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Publications
Targeting, imaging, and therapy using a humanized antiprostate stem cell antigen (PSCA) antibodyTove Olafsen
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
J Immunother 30:396-405. 2007..These data support further development of hu1G8 anti-PSCA antibody for targeted imaging and therapy for tumors of urogenital origin...- Protein targeting constructs in alpha therapyTove Olafsen
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Curr Radiopharm 4:197-213. 2011..e. diabody) and tetramer, CH2 domain deleted antibody fragments); other protein targeting constructs such as affibodies and peptides as well as liposomal delivery... - Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targetingTove Olafsen
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 700 Westwood Plaza, Los Angeles, CA 90095, USA
Protein Eng Des Sel 17:315-23. 2004..62 h) was similar. Thus, minibodies with different specificities display similar pharmacokinetics, while tumor uptake may vary depending on the antigen-antibody system... - Recombinant anti-CD20 antibody fragments for small-animal PET imaging of B-cell lymphomasTove Olafsen
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA
J Nucl Med 50:1500-8. 2009..To provide more sensitive, tumor-specific PET imaging of NHL, we sought to develop PET agents targeting CD20... - Optimizing radiolabeled engineered anti-p185HER2 antibody fragments for in vivo imagingTove Olafsen
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California at Los Angeles, 90095, USA
Cancer Res 65:5907-16. 2005..1 +/- 1.5% ID/g). Thus, by manipulating the size and format of anti-p185(HER2) antibody fragments, the kidney activity was reduced and high or low expression of p185HER2 in xenografts could be distinguished by microPET imaging... - ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies)Tove Olafsen
Department of Molecular and Medical Pharmacology, UCLA Crump Institute for Molecular Imaging, Los Angeles, CA 90095, USA
Protein Eng Des Sel 23:243-9. 2010..Specific tumor targeting and high contrast images were achieved with the anti-CD20 diabodies. These agents extend the repertoire of reagents that can potentially be used to improve detection of low-grade lymphomas... - Antibody vectors for imagingTove Olafsen
UCLA Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA
Semin Nucl Med 40:167-81. 2010..The preclinical data presented are compelling, and it is evident that antibody-based molecular imaging tracers will play an important future role in the diagnosis and management of cancer and other diseases... - Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragmentTove Olafsen
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California Los Angeles, 700 Westwood Plaza, Los Angeles, California 90095, USA
Nat Protoc 1:2048-60. 2006..The time frame for completing the procedure is about 6 months, provided that no complications are encountered... - Radioiodinated versus radiometal-labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: optimal pharmacokinetics for therapyVania Kenanova
Division of Molecular Biology, Beckman Research Institute of the City of Hope, Department of Radioimmunotherapy, Duarte, CA, USA
Cancer Res 67:718-26. 2007..Finally, the 125I/111In biodistribution data allowed for dose estimations, which suggest the 131I-labeled scFv-Fc H310A/H435Q as a promising candidate for radioimmunotherapy... - Humanized radioiodinated minibody for imaging of prostate stem cell antigen-expressing tumorsJeffrey V Leyton
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA
Clin Cancer Res 14:7488-96. 2008..In this study, the 2B3 minibody (an 80-kDa engineered antibody fragment) has been produced for rapid targeting and imaging... - Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applicationsTove Olafsen
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095
Protein Eng Des Sel 17:21-7. 2004..This format will provide a versatile platform for targeting a variety of agents to CEA-positive tumors... - Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imagingKaty M Venisnik
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Mol Imaging Biol 9:267-77. 2007..4% ID/g) at 21 hours. Although further optimization of this fusion protein may be needed to improve in vivo performance, the diabody-GLDelta15 is a promising optical imaging probe for tumor detection in vivo... - Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragmentsVania Kenanova
Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA
Cancer Res 65:622-31. 2005..Thus, attenuating the Fc-FcRn interaction provides a way of controlling the antibody fragment serum half-life without compromising expression and tumor targeting... - Engineered humanized diabodies for microPET imaging of prostate stem cell antigen-expressing tumorsJeffrey V Leyton
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Protein Eng Des Sel 22:209-16. 2009..Although the higher affinity bm2B3-Db8 demonstrated improved tumor retention at later time points (20 h), it did not improve tumor targeting or imaging compared with p2B3-Db8 at 12 h... - Tuning the serum persistence of human serum albumin domain III:diabody fusion proteinsVania E Kenanova
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, California NanoSystems Institute, University of California, Los Angeles, 570 Westwood Plaza, CA 90095 1770, USA
Protein Eng Des Sel 23:789-98. 2010..H464 appears to be most crucial for FcRn binding (greatest reduction in MRT), followed by H510 and H535. By mutating the DIII scaffold, we can dial serum kinetics for imaging or therapy applications... - Anti-CA19-9 diabody as a PET imaging probe for pancreas cancerMark D Girgis
Department of Surgery, UCLA, Los Angeles, California 90095, USA
J Surg Res 170:169-78. 2011..Our goal was to develop and characterize the anti-CA19-9 diabody fragment and determine its ability to provide antigen specific imaging of pancreas cancer... - Engineered antibody fragments with infinite affinity as reporter genes for PET imagingLiu H Wei
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
J Nucl Med 49:1828-35. 2008..The objective of the present study was to evaluate a new approach for reporter gene imaging based on cell surface expression of antibody fragments that can irreversibly bind to radiometal chelates... - An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumorsEric J Lepin
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
Eur J Nucl Med Mol Imaging 37:1529-38. 2010..However, the humanization and antibody fragment reformatting decreased its apparent affinity. Here, we sought to evaluate PET imaging contrast with affinity matured minibodies... - Site-specific, thiol-mediated conjugation of fluorescent probes to cysteine-modified diabodies targeting CD20 or HER2Shannon J Sirk
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
Bioconjug Chem 19:2527-34. 2008..Conjugates retained both specificity and fluorescence, demonstrating the successful expansion of the CysDb repertoire to new targets and to new site-specific conjugation possibilities... - Recombinant carcinoembryonic antigen as a reporter gene for molecular imagingVania Kenanova
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, 700 Westwood Plaza, Los Angeles, CA 90095, USA
Eur J Nucl Med Mol Imaging 36:104-14. 2009..We have developed a reporter gene based on carcinoembryonic antigen (CEA), a human protein with limited normal tissue expression... - Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumorsKaty M Venisnik
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Protein Eng Des Sel 19:453-60. 2006.... - Cys-diabody quantum dot conjugates (immunoQdots) for cancer marker detectionBhaswati Barat
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
Bioconjug Chem 20:1474-81. 2009.... - Evaluation of two internalizing carcinoembryonic antigen reporter genes for molecular imagingBhaswati Barat
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1770, USA
Mol Imaging Biol 13:526-35. 2011..The objective of this article is to develop internalizing positron emission tomography (PET) reporter genes for tracking genetically modified T cells in vivo... - Antibodies for molecular imaging of cancerAnna M Wu
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
Cancer J 14:191-7. 2008..Antibody imaging can provide a sensitive, noninvasive means for molecular characterization of cell surface phenotype in vivo, which can in turn guide diagnosis, prognosis, therapy selection, and monitoring of treatment in cancer... - Targeting CEA in Pancreas Cancer Xenografts with a Mutated scFv-Fc Antibody FragmentMark D Girgis
Department of Surgery, UCLA, 10833 LeConte Ave, Rm 54 140, Los Angeles, CA 90095, USA
EJNMMI Res 1:24. 2011..abstract:.. - CA19-9 as a Potential Target for Radiolabeled Antibody-Based Positron Emission Tomography of Pancreas CancerMark D Girgis
Department of Surgery, UCLA, 10833 LeConte Avenue, Rm 54 140, Los Angeles, CA 90095, USA
Int J Mol Imaging 2011:834515. 2011..Conclusion. We show in vivo targeting of our antigen positive xenograft with a radiolabeled anti-CA19-9 antibody. These data demonstrate the potential to achieve anti-CA19-9 antibody based positron emission tomography of pancreas cancer... - Unexpected expression pattern for glycosylphosphatidylinositol-anchored HDL-binding protein 1 (GPIHBP1) in mouse tissues revealed by positron emission tomography scanningTove Olafsen
Department of Molecular and Medical Pharmacology, California NanoSystems Institute, Los Angeles, California 90095, USA
J Biol Chem 285:39239-48. 2010..In support of this concept, lung LPL levels were significantly lower in Gpihbp1(-/-) mice than in Gpihbp1(+/+) mice. In addition, Lpl(-/-) mice expressing human LPL exclusively in muscle contained high levels of human LPL in the lung... - ImmunoPET using engineered antibody fragments: fluorine-18 labeled diabodies for same-day imagingTove Olafsen
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, CNSI 4335, Box 951770, Los Angeles, CA, 90095 1770, USA
Tumour Biol 33:669-77. 2012..This approach can be broadly applied to antibodies targeting cell surface biomarkers for molecular imaging of tumors and should be highly translatable for clinical use... - Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPTSepideh Afshar
Department of Microbiology, Immunology, and Molecular Genetics, UCLA, 247 BSRB, 615 Charles E, Young East, Los Angeles, 90095, USA
J Exp Clin Cancer Res 28:147. 2009..abstract:.. - The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entryKouki Morizono
Division of Hematology and Oncology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
Cell Host Microbe 9:286-98. 2011..These results reveal an alternative molecular mechanism of viral entry that can broaden host range and enhance infectivity of enveloped viruses... - PET imaging of colorectal cancer in xenograft-bearing mice by use of an 18F-labeled T84.66 anti-carcinoembryonic antigen diabodyWeibo Cai
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, Stanford, California 94305 5484, USA
J Nucl Med 48:304-10. 2007..66 diabody, a genetically engineered noncovalent dimer of single-chain variable fragments, for small-animal PET imaging of CEA expression in xenograft-bearing mice... - Reduction of kidney uptake in radiometal labeled peptide linkers conjugated to recombinant antibody fragments. Site-specific conjugation of DOTA-peptides to a Cys-diabodyLin Li
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
Bioconjug Chem 13:985-95. 2002..Furthermore, we demonstrated a role for the attachment chemistry to the epsilon-amino group of the lysine on the magnitude of kidney uptake...