KEITH W NEHRKE

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. ncbi A reduction in intestinal cell pHi due to loss of the Caenorhabditis elegans Na+/H+ exchanger NHX-2 increases life span
    Keith Nehrke
    Gastroenterology Unit, Department of Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 278:44657-66. 2003
  2. ncbi Regulation of acid-base transporters by reactive oxygen species following mitochondrial fragmentation
    David Johnson
    Dept of Medicine, Nephrology Division, Univ of Rochester Medical Center Box 675, 601 Elmwood Ave, Rochester NY 14642
    Am J Physiol Cell Physiol 302:C1045-54. 2012
  3. ncbi Mitochondrial fragmentation leads to intracellular acidification in Caenorhabditis elegans and mammalian cells
    David Johnson
    Department of Biochemistry, Medicine, and Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Biol Cell 21:2191-201. 2010
  4. ncbi Intestinal Ca2+ wave dynamics in freely moving C. elegans coordinate execution of a rhythmic motor program
    K Nehrke
    Dept of Medicine, Nephrology Division, Medical Center Box 675, 601 Elmwood Ave, Rochester NY 14642, USA
    Am J Physiol Cell Physiol 294:C333-44. 2008
  5. ncbi The NHX family of Na+-H+ exchangers in Caenorhabditis elegans
    Keith Nehrke
    Center for Oral Biology, Aab Institute of Biomedical Sciences and the Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:29036-44. 2002
  6. ncbi A novel mitochondrial K(ATP) channel assay
    Andrew P Wojtovich
    Department of Pharmacology, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Circ Res 106:1190-6. 2010
  7. ncbi The mitochondrial complex II and ATP-sensitive potassium channel interaction: quantitation of the channel in heart mitochondria
    Andrew P Wojtovich
    Department of Anesthesiology, University of Rochester Medical Center, NY, USA
    Acta Biochim Pol 57:431-4. 2010
  8. ncbi Targeted disruption of the Nhe1 gene fails to inhibit beta(1)-adrenergic receptor-induced parotid gland hypertrophy
    J E Melvin
    Center for Oral Biology, Aab Institute of Biomedical Sciences, Rochester, New York 14642, USA
    Am J Physiol Gastrointest Liver Physiol 280:G694-700. 2001
  9. ncbi cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans
    F K Hagen
    Center for Oral Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    J Biol Chem 273:8268-77. 1998
  10. ncbi Defective fluid secretion and NaCl absorption in the parotid glands of Na+/H+ exchanger-deficient mice
    K Park
    Center for Oral Biology, Rochester Institute of Biomedical Sciences, and the Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 276:27042-50. 2001

Research Grants

  1. Molecular Basis of K+ Current During Secretion
    Keith Nehrke; Fiscal Year: 2002
  2. Na+/H+ exchange in the nematode intestine
    Keith Nehrke; Fiscal Year: 2004
  3. pH Regulation of Fat Storage in the Nematode Intestine
    Keith Nehrke; Fiscal Year: 2007
  4. Neuroprotection by a Nuclear Carbonic Anhydrase in C. elegans
    KEITH W NEHRKE; Fiscal Year: 2010

Collaborators

Detail Information

Publications13

  1. ncbi A reduction in intestinal cell pHi due to loss of the Caenorhabditis elegans Na+/H+ exchanger NHX-2 increases life span
    Keith Nehrke
    Gastroenterology Unit, Department of Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 278:44657-66. 2003
    ..Our data demonstrate a functional role for a Na+/H+ exchanger in nutrient absorption in vivo and lays the groundwork for examining integrated acid-base physiology in a non-mammalian model organism...
  2. ncbi Regulation of acid-base transporters by reactive oxygen species following mitochondrial fragmentation
    David Johnson
    Dept of Medicine, Nephrology Division, Univ of Rochester Medical Center Box 675, 601 Elmwood Ave, Rochester NY 14642
    Am J Physiol Cell Physiol 302:C1045-54. 2012
    ..Because maintaining pH homeostasis is essential for cell function and viability, the results of this work provide new insight into the pathophysiology associated with the loss of inner mitochondrial membrane fusion...
  3. ncbi Mitochondrial fragmentation leads to intracellular acidification in Caenorhabditis elegans and mammalian cells
    David Johnson
    Department of Biochemistry, Medicine, and Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Mol Biol Cell 21:2191-201. 2010
    ..Taken together these data reveal a potential role for acidosis in the differing etiology of diseases associated with mitochondrial morphology defects such as ADOA and CMT-2A...
  4. ncbi Intestinal Ca2+ wave dynamics in freely moving C. elegans coordinate execution of a rhythmic motor program
    K Nehrke
    Dept of Medicine, Nephrology Division, Medical Center Box 675, 601 Elmwood Ave, Rochester NY 14642, USA
    Am J Physiol Cell Physiol 294:C333-44. 2008
    ..Finally, our results demonstrate that it is possible to assess Ca(2+) dynamics and muscular contractions in a completely unrestrained model organism...
  5. ncbi The NHX family of Na+-H+ exchangers in Caenorhabditis elegans
    Keith Nehrke
    Center for Oral Biology, Aab Institute of Biomedical Sciences and the Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:29036-44. 2002
    ..NHX-4 has a K(a) for Na+ of approximately 32 mm, is not Cl- -dependent, and is relatively insensitive to the amiloride analog EIPA...
  6. ncbi A novel mitochondrial K(ATP) channel assay
    Andrew P Wojtovich
    Department of Pharmacology, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, NY 14642, USA
    Circ Res 106:1190-6. 2010
    ..The validity of current methods to assay mK(ATP) activity is disputed...
  7. ncbi The mitochondrial complex II and ATP-sensitive potassium channel interaction: quantitation of the channel in heart mitochondria
    Andrew P Wojtovich
    Department of Anesthesiology, University of Rochester Medical Center, NY, USA
    Acta Biochim Pol 57:431-4. 2010
    ..4 % of total complex II molecules are necessary to activate the mK(ATP). These results estimate the mK(ATP) content at 15 channels per mitochondrion...
  8. ncbi Targeted disruption of the Nhe1 gene fails to inhibit beta(1)-adrenergic receptor-induced parotid gland hypertrophy
    J E Melvin
    Center for Oral Biology, Aab Institute of Biomedical Sciences, Rochester, New York 14642, USA
    Am J Physiol Gastrointest Liver Physiol 280:G694-700. 2001
    ..These data directly demonstrate that acinar cell hypertrophy induced by chronic beta(1)-adrenergic receptor stimulation occurs independently of NHE1 activity...
  9. ncbi cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans
    F K Hagen
    Center for Oral Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    J Biol Chem 273:8268-77. 1998
    ..elegans suggests that mucin O-glycosylation is catalyzed by a complex gene family, which is conserved among evolutionary-distinct organisms...
  10. ncbi Defective fluid secretion and NaCl absorption in the parotid glands of Na+/H+ exchanger-deficient mice
    K Park
    Center for Oral Biology, Rochester Institute of Biomedical Sciences, and the Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 276:27042-50. 2001
    ..These results also demonstrate that basolateral NHE1 and apical NHE2 modulate saliva secretion in vivo, especially during sustained stimulation when secretion depends less on Na(+)/K(+)/2Cl(-) cotransporter activity...
  11. ncbi The C. elegans mitochondrial K+(ATP) channel: a potential target for preconditioning
    Andrew P Wojtovich
    Department of Pharmacology and Physiology, Box 604, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 376:625-8. 2008
    ..Since C. elegans also exhibit IPC, our observations provide a framework to study the role of mK(ATP) in IR injury in a genetic model organism...
  12. ncbi Acute inhibition of brain-specific Na(+)/H(+) exchanger isoform 5 by protein kinases A and C and cell shrinkage
    S Attaphitaya
    Center for Oral Biology in the Aab Institute of Biomedical Sciences, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Am J Physiol Cell Physiol 281:C1146-57. 2001
    ..These results demonstrate that rat brain NHE5 is downregulated by activation of PKA and PKC and by cell shrinkage, important regulators of neuronal cell function...
  13. ncbi Charge distribution of flanking amino acids inhibits O-glycosylation of several single-site acceptors in vivo
    K Nehrke
    Department of Dental Research, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
    Glycobiology 7:1053-60. 1997
    ..Charge distribution would appear, therefore, to play a more general role in determining the extent to which solitary O-glycosylation sites are modified...

Research Grants7

  1. Molecular Basis of K+ Current During Secretion
    Keith Nehrke; Fiscal Year: 2002
    ..Finally, we will look at compensatory changes that occur in the expression of functionally-related ion transport proteins in the transgenic strain. ..
  2. Na+/H+ exchange in the nematode intestine
    Keith Nehrke; Fiscal Year: 2004
    ..This multidisciplinary approach will help to define the biological role of individual Na+/H+ exchangers in the nematode intestine. ..
  3. pH Regulation of Fat Storage in the Nematode Intestine
    Keith Nehrke; Fiscal Year: 2007
    ..The strength of this proposal is that it will use a powerful model system and novel reagents for monitoring pH in living organisms to study fat biology from a unique perspective: that of acid-base homeostasis. ..
  4. Neuroprotection by a Nuclear Carbonic Anhydrase in C. elegans
    KEITH W NEHRKE; Fiscal Year: 2010
    ..This application is focused on understanding the mechanism behind nuclear CAs protective effects in nematodes and translating these findings to mammals as a way of targeting cognitive impairment. ..