Research Topics
| Sharon MurphySummaryAffiliation: University of Minnesota Country: USA Publications
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Detail Information
Publications
Consumption of watercress fails to alter coumarin metabolism in humansS E Murphy
University of Minnesota Cancer Center, Minneapolis 55455, USA
Drug Metab Dispos 29:786-8. 2001..Therefore, under the conditions of our study, PEITC and other constituents of watercress had at most a marginal inhibitory effect on P450 2A6-catalyzed coumarin 7-hydroxylation...- Analysis of [3',3'-d(2)]-nicotine and [3',3'-d(2)]-cotinine by capillary liquid chromatography-electrospray tandem mass spectrometrySharon E Murphy
University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
J Chromatogr B Analyt Technol Biomed Life Sci 857:1-8. 2007..Preliminary analysis of a subset of subjects from these studies detected a significantly lower rate of nicotine conversion to cotinine by past smokers compared to current smokers... - Nicotine 5'-oxidation and methyl oxidation by P450 2A enzymesSharon E Murphy
The Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware St SE, Minneapolis, MN 55455, USA
Drug Metab Dispos 33:1166-73. 2005..Therefore, methyl oxidation of nicotine by P450 2A6 or P450 2A13 followed by nitrosation of nornicotine are possible endogenous pathways of N'-nitrosonornicotine formation... - Functional characterization of CYP2A13 polymorphismsK E Schlicht
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
Xenobiotica 37:1439-49. 2007..Although these changes are not likely to impact in vivo metabolism, these data should aid in the interpretation and design of future epidemiology studies... - A comparison of urinary biomarkers of tobacco and carcinogen exposure in smokersSharon E Murphy
Transdisciplinary Tobacco Use Research Center, Minneapolis, MN 55455, USA
Cancer Epidemiol Biomarkers Prev 13:1617-23. 2004..Whereas urinary concentrations of 1-hydroxypyrene were consistent across the four visits, the levels were not correlated with NNAL, anatabine, nicotine, or any nicotine metabolites... - Chronic nicotine consumption does not influence 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesisSharon E Murphy
Department of Biochemistry Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Cancer Prev Res (Phila) 4:1752-60. 2011..These negative findings in a commonly used model of human lung carcinogenesis should lead us to question the interpretation of the many in vitro studies that find that nicotine stimulates cancer cell growth... - Pilot study on lower nitrosamine smokeless tobacco products compared with medicinal nicotineM Irene Mendoza Baumgart
Transdisciplinary Tobacco Use Research Center, Cancer Center, University of Minnesota, Minneapolis, MN 55414, USA
Nicotine Tob Res 9:1309-23. 2007..However, the population effect of marketing of such products as reduced exposure/reduced risk is unknown. The need for further research in this area and regulation of tobacco products is evident... - Urinary metabolites of a tobacco-specific lung carcinogen in nonsmoking hospitality workersOzlem E Tulunay
Tobacco Use Research Center, University of Minnesota, 2701 University Avenue Southeast, 201, Minneapolis, MN 55414, USA
Cancer Epidemiol Biomarkers Prev 14:1283-6. 2005..The results of this study show that smoke exposure in bars and restaurants may have important health effects on nonsmoking employees, elicited by the increase in carcinogen levels... - Effects of high dose transdermal nicotine replacement in cigarette smokersDorothy Hatsukami
Transdisciplinary Tobacco Use Research Center, University of Minnesota, Department of Psychiatry, Minneapolis, MN 55414, United States
Pharmacol Biochem Behav 86:132-9. 2007..In most of these studies, only conventional doses of NRT have been tested and higher doses may be required to result in significant reductions in smoking and in biomarkers of exposure... - Relationships between cigarette consumption and biomarkers of tobacco toxin exposureAnne M Joseph
Section of General Internal Medicine 111 0, VA Medical Center, One Veterans Drive, Minneapolis, MN 55417, USA
Cancer Epidemiol Biomarkers Prev 14:2963-8. 2005.... - Tobacco smoke exposure in nonsmoking hospitality workers before and after a state smoking banJoni A Jensen
Tobacco Use Research Center, University of Minnesota, 717 Delaware Street South East, Minneapolis, MN 55414, USA
Cancer Epidemiol Biomarkers Prev 19:1016-21. 2010..These results provide further support for the importance of protecting employees working in all venues... - Effects of cigarette reduction on cardiovascular risk factors and subjective measuresDorothy K Hatsukami
Transdisciplinary Tobacco Use Research Center, University of Minnesota, 2701 University Ave SE, Minneapolis, MN 55414, USA
Chest 128:2528-37. 2005..To assess the effect of continued smoking and smoking reduction on cardiovascular biomarkers (eg, WBC count, cholesterol concentrations, BP, heart rate)... - Inactivation of CYP2A6 and CYP2A13 during nicotine metabolismLinda B von Weymarn
The Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street S.E, Minneapolis, MN 55455, USA
J Pharmacol Exp Ther 316:295-303. 2006..Studies are on-going to identify the metabolite responsible for nicotine-mediated inactivation of CYP2A13... - Nicotine metabolism in African Americans and European Americans: variation in glucuronidation by ethnicity and UGT2B10 haplotypeJeannette Zinggeler Berg
Department of Biochemistry, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
J Pharmacol Exp Ther 332:202-9. 2010..Second, we provide the first convincing evidence that UGT2B10 is a key catalyst of these glucuronidation pathways in vivo... - Preferential metabolic activation of N-nitrosopiperidine as compared to its structural homologue N-nitrosopyrrolidine by rat nasal mucosal microsomesHansen L Wong
University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA
Chem Res Toxicol 16:1298-305. 2003..These results are consistent with local metabolic activation of nitrosamines as a contributing factor in their tissue-specific carcinogenicity... - Exposure to a tobacco-specific lung carcinogen in adolescent versus adult smokersLouise A Hertsgaard
University of Minnesota Transdisciplinary Tobacco Use Research Center, 2701 University Avenue Southeast, 201, Minneapolis, MN 55414, USA
Cancer Epidemiol Biomarkers Prev 17:3337-43. 2008..This study compared carcinogen and nicotine exposure in adolescent and adult smokers across categories of CPD... - Urinary levels of tobacco-specific nitrosamine metabolites in relation to lung cancer development in two prospective cohorts of cigarette smokersJian Min Yuan
Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
Cancer Res 69:2990-5. 2009..Findings of the present study directly link NNK exposure to lung cancer development in humans... - 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides in the urine of infants exposed to environmental tobacco smokeStephen S Hecht
The Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street Southeast, Minneapolis, MN 55455, USA
Cancer Epidemiol Biomarkers Prev 15:988-92. 2006..The results of this study show substantial uptake of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in infants exposed to ETS and support the concept that persistent ETS exposure in childhood could be related to cancer later in life... - Abstinence and relapse rates following a college campus-based quit & win contestJanet L Thomas
Department of Medicine, Division of General Internal Medicine, University of Minnesota, Minneapolis 55414, USA
J Am Coll Health 58:365-72. 2010..To conduct and evaluate Quit & Win contests at 2 2-year college and 2 4-year university campuses... - Stability of the nicotine metabolite ratio in ad libitum and reducing smokersMarc E Mooney
University of Minnesota, Transdisciplinary Tobacco Use Research Center, 2701 University Avenue South East, Suite 201, Minneapolis, MN 55414, USA
Cancer Epidemiol Biomarkers Prev 17:1396-400. 2008..Our objective was to evaluate the correlates and stability of the 3-HC to cotinine ratio in ad libitum and reducing smokers, using nicotine replacement therapy (NRT), over a period of months... - Smoking reduction fails to improve clinical and biological markers of cardiac disease: a randomized controlled trialAnne M Joseph
Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55414, USA
Nicotine Tob Res 10:471-81. 2008..These results emphasize the importance of abstinence for smokers with heart disease to minimize health risks from tobacco... - Effect of oral snus and medicinal nicotine in smokers on toxicant exposure and withdrawal symptoms: a feasibility studyMichael Kotlyar
University of Minnesota Tobacco Use Research Center, University of Minnesota, Minneapolis, Minnesota, USA
Cancer Epidemiol Biomarkers Prev 20:91-100. 2011..Data are needed regarding how smokers interested in cessation would use these products, the levels of resultant toxicant exposure, and the feasibility of using these products as aids for tobacco cessation... - Developing the science base for reducing tobacco harmDorothy K Hatsukami
University of Minnesota Transdisciplinary Tobacco Use Research Center, Minneapolis, MN 55414, USA
Nicotine Tob Res 9:S537-53. 2007..A description of the ongoing activities and challenges in these areas is provided, along with projected directions for the future... - Similar exposure to a tobacco-specific carcinogen in smokeless tobacco users and cigarette smokersStephen S Hecht
Cancer Center and Transdisciplinary Tobacco Use Research Center, University of Minnesota Cancer Center, 420 Delaware Street Southeast, MMC 806, Minneapolis, MN 55455, USA
Cancer Epidemiol Biomarkers Prev 16:1567-72. 2007..001). These results show similar exposures to the potent tobacco-specific carcinogen NNK in smokeless tobacco users and smokers. These findings do not support the use of smokeless tobacco as a safe substitute for smoking... - Reduced nicotine content cigarettes: effects on toxicant exposure, dependence and cessationDorothy K Hatsukami
University of Minnesota Transdisciplinary Tobacco Use Research Center, Minneapolis, MN 55414, USA
Addiction 105:343-55. 2010..To examine the effects of reduced nicotine cigarettes on smoking behavior, toxicant exposure, dependence and abstinence... - Comparative metabolism of the tobacco-specific nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol by rat cytochrome P450 2A3 and human cytochrome P450 2A13John R Jalas
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
Drug Metab Dispos 31:1199-202. 2003.... - Effects of reduced cigarette smoking on levels of 1-hydroxypyrene in urineStephen S Hecht
Transdisciplinary Tobacco Use Research Center and Cancer Center, University of Minnesota, Mayo Mail Code 806, 420 Delaware Street SE, Minneapolis, MN 55455, USA
Cancer Epidemiol Biomarkers Prev 13:834-42. 2004.... - Effects of nicotine infusion on the metabolism of the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in ratsDaniel Keyler
College of Pharmacy, University of Minnesota, 308 Harvard Street SE, Minneapolis, MN 55455, USA
Cancer Lett 202:1-9. 2003.... - N-glucuronidation of trans-3'-hydroxycotinine by human liver microsomesGwendolyn E Kuehl
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA
Chem Res Toxicol 16:1502-6. 2003.... - Metabolites of a tobacco-specific lung carcinogen in nonsmoking casino patronsKristin E Anderson
Division of Epidemiology, Comprehensive Cancer Center, University of Minnesota, Minneapolis, Minnesota 55454, USA
Cancer Epidemiol Biomarkers Prev 12:1544-6. 2003..044 nmol/mg creatinine, P < 0.0001) and total NNAL (0.018 pmol/mg creatinine, P < 0.001). These findings demonstrate that exposure of nonsmokers to ETS in a commercial setting results in uptake of a tobacco-specific lung carcinogen... - Effects of reduced cigarette smoking on the uptake of a tobacco-specific lung carcinogenStephen S Hecht
Transdisciplinary Tobacco Use Research Center and College of Pharmacy, University of Minnesota, Minneapolis, USA
J Natl Cancer Inst 96:107-15. 2004.... - Evaluation of carcinogen exposure in people who used "reduced exposure" tobacco productsDorothy K Hatsukami
University of Minnesota Transdisciplinary Tobacco Use Research Center and Cancer Center, Minneapolis 55414, USA
J Natl Cancer Inst 96:844-52. 2004..Although tobacco products with reportedly reduced carcinogen content are being marketed, carcinogen uptake in people who use these products has not been assessed systematically... - Prevalence of cigarette smoking in pregnant women participating in the special supplemental nutrition programme for Women, Infants and Children (WIC) in Minneapolis and Saint Paul, Minnesota, USAJulie A Ross
Division of Pediatric Epidemiology and Clinical Research, University of Minnesota Department of Pediatrics, 420 Delaware Street SE, Minneapolis, MN 55455, USA
Paediatr Perinat Epidemiol 16:246-8. 2002..These data suggest that some participants in WIC make a concerted effort to quit smoking when they find out they are pregnant, and are generally truthful when reporting their smoking habits during pregnancy... - Similar uptake of lung carcinogens by smokers of regular, light, and ultralight cigarettesStephen S Hecht
Transdiciplinary Tobacco Use Research and Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
Cancer Epidemiol Biomarkers Prev 14:693-8. 2005..The results are consistent with epidemiologic studies that show no difference in lung cancer risk in smokers of these cigarettes... - Comparative metabolism of N-nitrosopiperidine and N-nitrosopyrrolidine by rat liver and esophageal microsomes and cytochrome P450 2A3Hansen L Wong
University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
Carcinogenesis 24:291-300. 2003..These results are consistent with previous findings that tissue-specific activation of nitrosamines contributes to tissue-specific tumor formation... - Nicotine and 4-(methylnitrosamino)-1-(3-pyridyl)-butanone metabolism by cytochrome P450 2B6Kari E Dicke
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis 55455, USA
Drug Metab Dispos 33:1760-4. 2005..However, P450 2B6 contributed significantly to NNK metabolism in HLMs expressing both P450 2B6 and P450 2A6, suggesting a possible role for P450 2B6 in NNK metabolic activation... - Metabolic activation of the tobacco carcinogen 4-(methylnitrosamino)-(3-pyridyl)-1-butanone by cytochrome P450 2A13 in human fetal nasal microsomesHansen L Wong
The Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
Chem Res Toxicol 18:913-8. 2005..The results are also the first to demonstrate high efficiency NNK alpha-hydroxylation in a human tissue... - Effect of CYP2A13 active site mutation N297A on metabolism of coumarin and tobacco-specific nitrosaminesKari E Schlicht
Department of Biochemistry Molecular Biology and Biophysics and Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
Drug Metab Dispos 37:665-71. 2009..In summary, Asn297 in P450 2A13 is important for orienting NNK and coumarin in the active site, changing this residue to Ala results in altered enzyme kinetics for NNK, NNN, and coumarin... - Cytochrome P450 enzymes as catalysts of metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a tobacco specific carcinogenJohn R Jalas
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
Chem Res Toxicol 18:95-110. 2005 - N-glucuronidation of nicotine and cotinine by human liver microsomes and heterologously expressed UDP-glucuronosyltransferasesGwendolyn E Kuehl
University of Minnesota Cancer Center, Department of Biochemistry, Molecular Biology and Biophysics, Minneapolis, Minnesota 55455, USA
Drug Metab Dispos 31:1361-8. 2003..Taken together, these data support a role for both UGT1A9 and UGT1A4 in the catalysis of nicotine and cotinine N-glucuronidation... - Cytochrome P450 2A-catalyzed metabolic activation of structurally similar carcinogenic nitrosamines: N'-nitrosonornicotine enantiomers, N-nitrosopiperidine, and N-nitrosopyrrolidineHansen L Wong
University of Minnesota, The Cancer Center, 420 Delaware Street SE, Mayo Mail Code 806, Minneapolis, Minnesota 55455, USA
Chem Res Toxicol 18:61-9. 2005..05), similar to previous reports with P450 2A3. Taken together, these data indicate that critical P450 2A residues determine the catalytic activities of NNN, NPIP, and NPYR alpha-hydroxylation... - Identification of N-(hydroxymethyl) norcotinine as a major product of cytochrome P450 2A6, but not cytochrome P450 2A13-catalyzed cotinine metabolismKathryn M Brown
The Cancer Center and the Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
Chem Res Toxicol 18:1792-8. 2005..This work has confirmed that the major metabolite of cotinine in smokers, trans-3'-hydroxycotinine, is only a minor metabolite of P450 2A6-catalyzed cotinine metabolism... - Smokeless tobacco topography and toxin exposureCharlotte A Lemmonds
Transdisciplinary Tobacco Use Research Center, University of Minnesota Cancer Center, Minneapolis 55414, USA
Nicotine Tob Res 7:469-74. 2005..001), particularly total dip duration, are significantly correlated with total cotinine, total nicotine, and total NNAL. These results suggest that how long a ST user has tobacco in his mouth is most predictive of tobacco toxin exposure... - UGT2B10 genotype influences nicotine glucuronidation, oxidation, and consumptionJeannette Zinggeler Berg
Department of Biochemistry, Molecular Biology, and Biophysics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
Cancer Epidemiol Biomarkers Prev 19:1423-31. 2010..We report here the effect of a polymorphism in a UDP-glucuronsyltransferase, UGT2B10, on nicotine metabolism and consumption... - CYP2A13: variable expression and role in human lung microsomal metabolic activation of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanoneXiuling Zhang
Wadsworth Center, New York State Department of Health, and School of Public Health, State University at New York, Albany, New York 12201 0509, USA
J Pharmacol Exp Ther 323:570-8. 2007..Therefore, individuals having relatively high levels of CYP2A13 expression will likely have an increased risk of developing smoking-related lung cancer... - Gene expression signature of cigarette smoking and its role in lung adenocarcinoma development and survivalMaria Teresa Landi
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health NIH, Department of Health and Human Services DHHS, Bethesda, Maryland, USA
PLoS ONE 3:e1651. 2008..Tobacco smoking is responsible for over 90% of lung cancer cases, and yet the precise molecular alterations induced by smoking in lung that develop into cancer and impact survival have remained obscure...
Research Grants
- Cytochrome P450 in Nicotine MetabolismSharon Murphy; Fiscal Year: 2007..Also of key importance, is that the inactivation of P450 2A enzymes will impact the metabolism of the tobacco specific carcinogens, NNK and NNN. ..
- Cytochrome P450 in Nicotine MetabolismSharon Murphy; Fiscal Year: 2004....
- Cytochrome P450 in Nicotine MetabolismSharon E Murphy; Fiscal Year: 2010..Also of key importance, is that the inactivation of P450 2A enzymes will impact the metabolism of the tobacco specific carcinogens, NNK and NNN. ..