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Genomes and GenesSpecies | Luis J MontanerSummaryAffiliation: University of Pennsylvania Country: USA Publications
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Publications
Type 1 and type 2 cytokine regulation of macrophage endocytosis: differential activation by IL-4/IL-13 as opposed to IFN-gamma or IL-10L J Montaner
The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 162:4606-13. 1999..In summary, our data document differential effects on macrophage endocytic functions by type 1 or type 2 cytokines associated with induction and effector pathways in immunity...
Structured treatment interruptions to control HIV-1 and limit drug exposureL J Montaner
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Trends Immunol 22:92-6. 2001..Such an approach has the ultimate objective of harnessing anti-viral immune responses, reducing drug exposure (toxicity and cost) and potentially extending the clinical benefits of a suppressive treatment regimen...
HIV type 1 viremia on ART is positively associated with polyclonal T cell proliferation in subjects with T cell IFN-gamma secretion levels comparable to those of uninfected subjectsEmmanouil Papasavvas
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
AIDS Res Hum Retroviruses 24:1203-8. 2008..This finding suggests that T cell hyperresponsiveness may play a role in the pathogenesis of immune comorbidities on ART...
Delayed loss of control of plasma lipopolysaccharide levels after therapy interruption in chronically HIV-1-infected patientsEmmanouil Papasavvas
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
AIDS 23:369-75. 2009..Increased circulating levels of lipopolysaccharide (LPS) have been demonstrated in HIV-1-infected progressors. We investigated the effect of antiretroviral therapy (ART) interruptions on plasma LPS levels...
IL-13 acutely augments HIV-specific and recall responses from HIV-1-infected subjects in vitro by modulating monocytesEmmanouil Papasavvas
The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 175:5532-40. 2005..Our data show that IL-13 is able to acutely enhance the function of the CD14(+) cell subset toward supporting Ag-specific cell-mediated responses in chronic HIV-1 infection...
Baseline viral load and immune activation determine the extent of reconstitution of innate immune effectors in HIV-1-infected subjects undergoing antiretroviral treatmentJihed Chehimi
HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 179:2642-50. 2007....
HIV-1-specific CD4+ T cell responses in chronically HIV-1 infected blippers on antiretroviral therapy in relation to viral replication following treatment interruptionEmmanouil Papasavvas
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Clin Immunol 26:40-54. 2006..04) with no difference between groups by week 24. These data support that blippers have higher anti-HIV lymphoproliferative responses while on ART but experience equal to higher viral rebound as compared to matched non-blippers upon TI...
Randomized, controlled trial of therapy interruption in chronic HIV-1 infectionEmmanouil Papasavvas
The Wistar Institute, Philadelphia, Pennsylvania, USA
PLoS Med 1:e64. 2004..Based on these findings, we suggest that further clinical research on the long-term consequences of TI strategies to decrease drug exposure is warranted...
Inability of plasmacytoid dendritic cells to directly lyse HIV-infected autologous CD4+ T cells despite induction of tumor necrosis factor-related apoptosis-inducing ligandJihed Chehimi
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Virol 84:2762-73. 2010..Taken together, our data show a lack of sustained high levels of soluble IFN-alpha in chronic HIV-1 infection in vivo and document a lack of direct PDC cytolytic activity against autologous infected or uninfected CD4(+) T cells...
Increased metallothionein gene expression, zinc, and zinc-dependent resistance to apoptosis in circulating monocytes during HIV viremiaAndrea D Raymond
The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104 4268, USA
J Leukoc Biol 88:589-96. 2010..Taken together, increases in MT gene expression and intracellular zinc levels may contribute directly to maintenance of an immune-activated monocyte by mediating an increased resistance to apoptosis during active HIV-1 viremia...
Early and delayed benefits of HIV-1 suppression: timeline of recovery of innate immunity effector cellsLivio Azzoni
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
AIDS 21:293-305. 2007..The kinetics of recovery for innate immune effectors following antiretroviral therapy are unknown...
Increased soluble vascular cell adhesion molecule-1 plasma levels and soluble intercellular adhesion molecule-1 during antiretroviral therapy interruption and retention of elevated soluble vascular cellular adhesion molecule-1 levels following resumption Emmanouil Papasavvas
The Wistar Institute, Philadelphia, PA 19104, USA
AIDS 22:1153-61. 2008....
DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivoMichael A Chattergoon
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Mol Ther 16:419-26. 2008....
Synthetic imidazoquinolines potently and broadly activate the cellular immune response of patients with cutaneous T-cell lymphoma: synergy with interferon-gamma enhances production of interleukin-12Maria Wysocka
Department of Dermatology, University of Pennsylvania, PA 19104, USA
Clin Lymphoma Myeloma 7:524-34. 2007....
NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cellsCostin Tomescu
HIV Immunopathogenesis Laboratory, Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 179:2097-104. 2007..Overall, our data suggest that HIV-infected cells are not innately resistant to NK lysis, and that exogenous NK stimulation derived from PDC can trigger NK cytotoxicity against HIV-1-infected autologous CD4(+) primary T cells...
Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational studyLivio Azzoni
HIV 1 Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
J Int AIDS Soc 14:37. 2011..We sought to determine if adiposity and molecules associated with lipid metabolism may affect the response to ART and the degree of subsequent immune reconstitution, and to assess their ability to predict CD4 recovery...
Circulating monocytes in HIV-1-infected viremic subjects exhibit an antiapoptosis gene signature and virus- and host-mediated apoptosis resistanceMalavika S Giri
The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 182:4459-70. 2009....
Co-immunization with plasmid IL-12 generates a strong T-cell memory response in miceMichael A Chattergoon
Department of Pathology, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 Stellar-Chance Laboratories, Philadelphia, PA 19104, USA
Vaccine 22:1744-50. 2004..The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen...
Advances in macrophage and dendritic cell biology in HIV-1 infection stress key understudied areas in infection, pathogenesis, and analysis of viral reservoirsLuis J Montaner
The Wistar Institute, Philadelphia, PA 19104 4268, USA
J Leukoc Biol 80:961-4. 2006....
Lack of persistent drug-resistant mutations evaluated within and between treatment interruptions in chronically HIV-1-infected patientsEmmanouil Papasavvas
Wistar Institute and the Philadelphia Field Initiating Group for HIV-1 Trials, Philadelphia, Pennsylvania, USA
AIDS 17:2337-43. 2003....
HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophagesJihed Chehimi
HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
J Leukoc Biol 74:757-63. 2003..39-48%), supporting the role of mannose-binding receptors in viral transmission. Taken together, results show that DC-SIGN regulation in MDM does not singly predict the transmission potential of this cell type...
Increased microbial translocation in ? 180 days old perinatally human immunodeficiency virus-positive infants as compared with human immunodeficiency virus-exposed uninfected infants of similar ageEmmanouil Papasavvas
The Wistar Institute, Philadelphia, PA, USA
Pediatr Infect Dis J 30:877-82. 2011....
Lessons learned from HIV treatment interruption: safety, correlates of immune control, and drug sparingLivio Azzoni
HIV Immunopathogenesis Laboratory, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Curr HIV Res 1:329-42. 2003..Based on ongoing research, we identify the topics to be targeted in future studies...
Association between HIV replication and serum leptin levels: an observational study of a cohort of HIV-1-infected South African womenLivio Azzoni
The Wistar Institute, Philadelphia, PA, USA
J Int AIDS Soc 13:33. 2010..Advanced HIV infection can result in lipoatrophy and wasting, even in the absence of ongoing opportunistic infections, suggesting that HIV may directly affect adipose tissue amount and distribution...
Flt3 ligand treatment reverses endotoxin tolerance-related immunoparalysisMaria Wysocka
Department of Dermatology, University of Pennsylvania, Philadelphia, 19104, USA
J Immunol 174:7398-402. 2005..Notably, administration of flt3 ligand rapidly reverses immunoparalysis in vivo, accelerating and amplifying repopulation of tissues with proinflammatory and immunoregulatory cytokine-producing dendritic cells...
Microarray data on gene modulation by HIV-1 in immune cells: 2000-2006Malavika S Giri
HIV Immunopathogenesis Laboratory, Wistar Institute, 3601 Spruce St, Room 480, Philadelphia, PA 19104, USA
J Leukoc Biol 80:1031-43. 2006....
Increased plasmacytoid dendritic cell maturation and natural killer cell activation in HIV-1 exposed, uninfected intravenous drug usersCostin Tomescu
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
AIDS 24:2151-60. 2010..However, the exact mechanism contributing to NK activation in HIV-1 exposed, uninfected intravenous drug users (EU-IDU) remains to be elucidated...
Retention of viability, cytotoxicity, and response to IL-2, IL-15, or IFN-alpha by human NK cells after CD107a degranulationCostin Tomescu
HIV Immunopathogenesis Laboratory, Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
J Leukoc Biol 85:871-6. 2009..Overall, our data argue for the strength of the NK cell compartment in the continuous surveillance of tumor and virally infected cells in the body and highlight the use of using CD107a expression as a stable marker for NK cytotoxicity...
Enhancement of the host immune responses in cutaneous T-cell lymphoma by CpG oligodeoxynucleotides and IL-15Maria Wysocka
Department of Dermatology, University of Pennsylvania, 245 CRB, 415 Curie Blvd, Philadelphia, PA 19104, USA
Blood 104:4142-9. 2004..These findings have important implications for the potential enhancement of antitumor immunity among patients with advanced CTCL...
Presence of human immunodeficiency virus-1-specific CD4 and CD8 cellular immune responses in children with full or partial virus suppressionEmmanouil Papasavvas
The Wistar Institute, Philadelphia, USA
J Infect Dis 188:873-82. 2003..Overall, a history of stable CD4(+) T cell counts, as a result of therapy that imparted full or partial virus suppression, was associated with increased levels of anti-HIV CD4(+) T helper responses and decreased T cell activation...
IL-12 suppression during experimental endotoxin tolerance: dendritic cell loss and macrophage hyporesponsivenessM Wysocka
The Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 166:7504-13. 2001..We find no demonstrable mechanistic role for B or T lymphocytes, the soluble mediators IL-10, TNF-alpha, IFN-alphabeta, or nitric oxide, or the NF-kappaB family members p50, p52, or RelB...
Costimulatory molecule immune enhancement in a plasmid vaccine model is regulated in part through the Ig constant-like domain of CD80/86Michael G Agadjanyan
Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA
J Immunol 171:4311-9. 2003....
IL-13 acts on macrophages to block the completion of reverse transcription, inhibit virus production, and reduce virus infectivityL J Montaner
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Leukoc Biol 62:126-32. 1997..62. Overall, our data indicate that IL-13 can act on macrophages before and after HIV-1 infection by blocking the completion of reverse transcription, decreasing virus production, and reducing the infectivity of the progeny virions...
Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophagesQi Luo
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 279:18451-6. 2004..The bifunctional control of IL-12 p40 by EKLF and its modulation of NFkappaB support a potential function for this factor in orchestrating IL-12 p40 production in macrophages...
HIV and cells of macrophage/dendritic lineage and other non-T cell reservoirs: new answers yield new questionsRonald G Collman
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
J Leukoc Biol 74:631-4. 2003..This issue of JLB contains several reviews and original reports resulting from the workshop that address recent progress and highlight the current research questions regarding these cell types...
CCR5 and CXCR4 expression correlated with X4 and R5 HIV-1 infection yet not sustained replication in Th1 and Th2 cellsM Moonis
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
AIDS 15:1941-9. 2001..ccr5 Delta 32-homozygous individuals maintain the ability for Th1/Th2 polarization, i.e., the expression of CCR5 is not required for Th1/Th2 polarization...
Role of interleukin-13 in innate and adaptive immunityJ O Brubaker
The Wistar Institute, Philadelphia, PA 19104, USA
Cell Mol Biol (Noisy-le-grand) 47:637-51. 2001..The role of this cytokine in immune responses is still being determined but evidence to date suggests this molecule has been conserved as an important regulatory factor involved in both early innate and late adaptive responses...
Dendritic and natural killer cell subsets associated with stable or declining CD4+ cell counts in treated HIV-1-infected childrenL Azzoni
Wistar Institute, Philadelphia, Pennsylvania 19104-4268, USA
J Infect Dis 191:1451-9. 2005..We hypothesize that the evaluation of selected innate-immunity effector cells may serve as a marker of CD4+ cell loss in pediatric HIV-1 infection...
Immune reconstitutionDrew Weissman
Division of Infectious Diseases, University of Pennsylvania, 522B Johnson Pavilion, Philadelphia, PA 19104, USA
Clin Lab Med 22:719-40. 2002....
Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individualsJihed Chehimi
HIV Immunopathogenesis Laboratory, Wistar Institute, Philadelphia, PA 19104, USA
J Immunol 168:4796-801. 2002..The persistent impairment of certain DC subsets may result in a sustained defect in DC-mediated innate immune functions despite an effective treatment regimen...
Sustained impairment of IFN-gamma secretion in suppressed HIV-infected patients despite mature NK cell recovery: evidence for a defective reconstitution of innate immunityLivio Azzoni
Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
J Immunol 168:5764-70. 2002....
HIV-1 Vpr regulates expression of beta chemokines in human primary lymphocytes and macrophagesK Muthumani
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, USA
J Leukoc Biol 68:366-72. 2000..These results suggest that a Vpr-mediated increase in virus replication could be in part through down-regulation of chemokine production...
Accumulation and intranuclear distribution of unintegrated human immunodeficiency virus type 1 DNAP Bell
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Virol 75:7683-91. 2001..Taken together, our results demonstrate that unintegrated proviral HIV-1 DNA does not randomly localize within infected cells but preferentially aggregates in the nucleus within SC35 domains...
HIV-1 viral protein R compromises cellular immune function in vivoVelpandi Ayyavoo
Department of Infectious Diseases and Microbiology, University of Pittsburgh, PA 15261, USA
Int Immunol 14:13-22. 2002..The data support that Vpr compromises antigen-specific immune responses and ultimately effector cell function, thus confirming a strong selective advantage to the virus at the expense of the host...
Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitroElizabeth J Soilleux
Department of Molecular Histopathology, University of Cambridge, United Kingdom
J Leukoc Biol 71:445-57. 2002....
