Research Topics
Genomes and GenesSpecies | H LueckeSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interactionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 293:1499-503. 2001..We propose that this residue interacts with the adjacent helices of the cognate NpSRII transducer NpHtrII...- Tritrichomonas foetus: a strategy for structure-based inhibitor design of a protozoan inosine-5'-monophosphate dehydrogenaseH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA
Exp Parasitol 87:203-11. 1997..Furthermore, a deep groove extends 8 A from the substrate phosphate away from the sugar. This structural uniqueness forms the basis of our efforts to design compounds that specifically inhibit the parasite enzyme... - Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 280:1934-7. 1998..The nearby Arg82 is the center of a network of numerous hydrogen-bonded residues and an ordered water molecule. This network defines the pathway of the proton from the buried Schiff base to the extracellular surface... - Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 286:255-61. 1999..The structural changes detected suggest the means for conserving energy at the active site and for ensuring the directionality of proton translocation... - Atomic resolution structures of bacteriorhodopsin photocycle intermediates: the role of discrete water molecules in the function of this light-driven ion pumpH Luecke
Departments of Molecular Biology and Biochemistry and Physiology and Biophysics, UCI Program in Macromolecular Structure, University of California, 92697 3900, Irvine, CA, USA
Biochim Biophys Acta 1460:133-56. 2000.... - Coupling photoisomerization of retinal to directional transport in bacteriorhodopsinH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 300:1237-55. 2000..This accounts for the lowering of the pK(a) of Asp96, which then reprotonates the Schiff base via a newly formed chain of water molecules that is extending toward the Schiff base... - Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediateS Rouhani
Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
J Mol Biol 313:615-28. 2001..On the cytoplasmic side, there is increased hydration near the surface, suggesting how Asp96 might communicate with the bulk during the rise of the O intermediate... - Structure of bacteriorhodopsin at 1.55 A resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA
J Mol Biol 291:899-911. 1999..A bilayer of 18 tightly bound lipid chains forms an annulus around the protein in the crystal. Contacts between the trimers in the membrane plane are mediated almost exclusively by lipids... - BacteriorhodopsinJ K Lanyi
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
Curr Opin Struct Biol 11:415-9. 2001.... - Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complexF G Whitby
Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 94143 0446, USA
Biochemistry 36:10666-74. 1997..This structural uniqueness may facilitate structure-based identification and design of compounds that specifically inhibit the parasite enzyme... - X-ray structure of full-length annexin 1 and implications for membrane aggregationA Rosengarth
Department of Molecular Biology and Biochemistry and UCI Program in Macromolecular Structure, University of California, 3205 Biological Sciences II, Irvine, CA, 92697 3900, USA
J Mol Biol 306:489-98. 2001..In turn, helix D is unwound into a flap now partially covering the N-terminal helix. Implications for membrane aggregation will be discussed and a model of aggregation based on the structure will be presented... - Annexins V and XII alter the properties of planar lipid bilayers seen by conductance probesY Sokolov
Department of Physiology and Biophysics, University of California, Irvine, Irvine, California 92697 4560, USA
J Gen Physiol 115:571-82. 2000.... - Structure of the human p53 core domain in the absence of DNAYing Wang
Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA 92697 3900, USA
Acta Crystallogr D Biol Crystallogr 63:276-81. 2007..The non-crystallographic tetramers display different protein contacts from those in other p53 crystals, giving rise to the question of how p53 arranges as a tetramer when it binds its target DNA... - UTP-bound and Apo structures of a minimal RNA uridylyltransferaseJason Stagno
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 366:882-99. 2007..We also report a 2.4 Angstroms crystal structure of TbTUT4 with the bound 2' deoxyribonucleoside, which provides the structural basis of the enzyme's preference toward ribonucleotides... - Ligand-free and -bound structures of the binding protein (LivJ) of the Escherichia coli ABC leucine/isoleucine/valine transport system: trajectory and dynamics of the interdomain rotation and ligand specificitySergei Trakhanov
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Biochemistry 44:6597-608. 2005..Results of normal-mode analysis and molecular dynamics simulations are consistent with the trajectory and intrinsic flexibility of the interdomain hinges and the dominance of one domain in ligand binding in the open state... - Crystal structure of complete rhinovirus RNA polymerase suggests front loading of protein primerTodd C Appleby
Valeant Pharmaceuticals International, Costa Mesa, California 92626, USA
J Virol 79:277-88. 2005..Based on the structural features of HRV16 3Dpo1 and the catalytic mechanism known for all polymerases, a front-loading model for uridylylation is proposed... - Anabaena sensory rhodopsin: a photochromic color sensor at 2.0 ALutz Vogeley
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 306:1390-3. 2004.... - Atomic resolution structures and the mechanism of ion pumping in bacteriorhodopsinBrian W Edmonds
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA
Front Biosci 9:1556-66. 2004..The precise mechanism of ion transfer in BR is therefore unresolved... - Structural and functional characterization of pi bulges and other short intrahelical deformationsJean-Philippe Cartailler
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA
Structure 12:133-44. 2004..Their stabilization commonly occurs via hydrogen bonding to water molecules or polar groups. Numerous deformations are implicated in basic yet vital functional roles, commonly as ligand binding site contributors... - Structural clues to the mechanism of ion pumping in bacteriorhodopsinHartmut Luecke
Departments of Biochemistry, Biophysics and Computer Science, University of California, Irvine, California 92697, USA
Adv Protein Chem 63:111-30. 2003 - X-ray crystallographic analysis of lipid-protein interactions in the bacteriorhodopsin purple membraneJean-Philippe Cartailler
Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California 92697-3900, USA
Annu Rev Biophys Biomol Struct 32:285-310. 2003..A brief discussion of the possibility that bacteriorhodopsin functions as a light-driven inward hydroxide pump rather than an outward proton pump is also presented... - Sensory rhodopsin II: functional insights from structureJohn L Spudich
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030, USA
Curr Opin Struct Biol 12:540-6. 2002.... - Crystal structure of Tritrichomonas foetus inosine monophosphate dehydrogenase in complex with the inhibitor ribavirin monophosphate reveals a catalysis-dependent ion-binding siteGlen L Prosise
Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA
J Biol Chem 277:50654-9. 2002..Exploitation of these differences may lead to novel inhibitors, which favor the microbial form of the enzyme... - Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanismGlen L Prosise
Department of Molecular Biology and Biochemistry University of California, Irvine, CA 92697-3900 USA
J Mol Biol 326:517-27. 2003..In addition, a potassium ion has been located at the dimer interface. A structural model for the kinetic mechanism is proposed... - A calcium-driven conformational switch of the N-terminal and core domains of annexin A1Anja Rosengarth
UCI Program in Macromolecular Structure, Department of Molecular Biology and Biochemistry, University of California, 3205 McGaugh Hall, Irvine, CA 92797-3900, USA
J Mol Biol 326:1317-25. 2003..Possible implications of this calcium-induced conformational switch for the membrane aggregation properties of annexin A1 will be discussed... - Annexin A1 interaction with a zwitterionic phospholipid monolayer: a fluorescence microscopy studyJ Alfredo Freites
Department of Physics and Astronomy and Institute for Surface and Interface Science, University of California, Irvine, California 92697-4575, USA
Langmuir 20:11674-83. 2004..Also, annexin A1 is found to form networks in the presence of a domain structure in the monolayer. Our results point toward an important contribution of the unique N-terminal domain to the organization of the protein at the interface... - Cloning, purification and crystallization of full-length human annexin 2Jacqueline T Tran
Department of Molecular Biology and Biochemistry, UCI Program in Macromolecular Structure, 3205 McGaugh Hall, University of California, Irvine, CA 92797-3900, USA
Acta Crystallogr D Biol Crystallogr 58:1854-7. 2002..0.2 and is 93.0% complete, with an R(sym) of 4.5%. The structure of full-length annexin 2 will provide insight into how its N-terminal domain contributes to its functional role in a variety of biological processes... - Crystal structure of the Anabaena sensory rhodopsin transducerLutz Vogeley
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 367:741-51. 2007..Possible mechanisms for the interaction of this transducer tetramer with the ASR photoreceptor via its flexible alpha-face to mediate transduction of the light signal are discussed... - Grafting segments from the extracellular surface of CCR5 onto a bacteriorhodopsin transmembrane scaffold confers HIV-1 coreceptor activityNajmoutin G Abdulaev
Center for Advanced Research in Biotechnology, National Institute of Standards and Technology and the University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
Structure 10:515-25. 2002..Methods developed here may be applicable to the dissection of functional domains from other seven-transmembrane receptors and form a basis for future structural studies... - Apo and calcium-bound crystal structures of Alpha-11 giardin, an unusual annexin from Giardia lambliaPuja Pathuri
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 368:493-508. 2007..Vesicle-binding studies suggest that alpha-11 giardin, unlike most other annexins, does not bind to vesicles composed of acidic phospholipids in a calcium-dependent manner... - Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferasesJason Stagno
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Proc Natl Acad Sci U S A 104:14634-9. 2007..We propose a dual role for RNA substrates in TUTase-catalyzed reactions: contribution to selective incorporation of the cognate nucleoside and shaping of the catalytic metal binding site... - Crystal structure of metastasis-associated protein S100A4 in the active calcium-bound formPuja Pathuri
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 383:62-77. 2008..The structure of the active form of S100A4 provides insight into its interactions with its binding partners and a better understanding of its role in metastasis...
Research Grants
- Structure-Function Studies of Annexin I and IIHartmut Luecke; Fiscal Year: 2006..5) Co-crystallize annexin with t-PA and solve the structure of this complex after characterizing complex formation in solution. ..
- The mechanism of signal transduction in photoreceptorsHartmut Luecke; Fiscal Year: 2006..5. Determine the crystal structure of the primary Anabaena photoreceptor in the ground and signaling state. 6. Determine the structure of the complex of the Anabaena photoreceptor with its putative transducer. ..
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2009....
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2010....
- STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLEHartmut Luecke; Fiscal Year: 2002....
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2009....