Research Topics
| Ziyi LiSummaryAffiliation: University of Iowa Country: USA Publications
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Detail Information
Publications
Nuclear transfer: progress and quandariesXuemei Li
Institute of Zoology, Chinese Academy of Science, Beijing 100080, PR China
Reprod Biol Endocrinol 1:84. 2003..This knowledge will help to identify the molecular basis of developmental defects seen in cloned embryos and provide methods for circumventing such problems associated with cloning the future application of this technology...
Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transferZiyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, 1 111 BSB, 51 Newton Road, Iowa City, IA 52242, USA
Reprod Biol Endocrinol 1:83. 2003..In this review, we will discuss our progress and the hurdles to NT cloning and gene-targeting that accompany efforts to generate animal models of genetic diseases in species such as the ferret...
Nuclear transfer of M-phase ferret fibroblasts synchronized with the microtubule inhibitor demecolcineZiyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA 52242, USA
J Exp Zool A Comp Exp Biol 303:1126-34. 2005..Our results should facilitate the development of cloned ferrets as an animal model for human lung disease such as influenza and cystic fibrosis...
Cloned ferrets produced by somatic cell nuclear transferZiyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Room 1-111 BSB, 51 Newton Road, Iowa City, IA 52242, USA
Dev Biol 293:439-48. 2006..Cloning of the domestic ferret may also aid in recovery and conservation of the endangered black-footed ferret and European mink...
Developmental capacity of ferret embryos by nuclear transfer using G0/G1-phase fetal fibroblastsZiyi Li
Departments of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Biol Reprod 68:2297-303. 2003....
Adeno-associated virus-targeted disruption of the CFTR gene in cloned ferretsXingshen Sun
Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
J Clin Invest 118:1578-83. 2008..To our knowledge, this study represents the first description of genetically engineered ferrets and describes an approach that may be of substantial utility in modeling not only CF, but also other genetic diseases...
Factors affecting the efficiency of embryo transfer in the domestic ferret (Mustela putorius furo)Ziyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, USA
Theriogenology 66:183-90. 2006....
Efficient term development of vitrified ferret embryos using a novel pipette chamber techniqueXingshen Sun
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Biol Reprod 79:832-40. 2008..These data indicate that the pipette chamber vitrification technique significantly improves the live birth rate of transferred ferret embryos relative to current state-of-the-art methods...
Factors affecting the electrofusion of mouse and ferret oocytes with ferret somatic cellsZiyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Mol Reprod Dev 72:40-7. 2005..These studies define conditions for efficient cell fusion with ferret oocytes and should facilitate SCNT and the development of genetically defined animal models in this species...
Targeted correction of single-base-pair mutations with adeno-associated virus vectors under nonselective conditionsXiaoming Liu
Department of Anatomy and Cell Biology and the Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
J Virol 78:4165-75. 2004..However, the failure of direct cytoplasm- or nucleus-injected rAAV DNA to facilitate gene repair suggests that some aspect of intracellular viral processing may be required to prime recombinant viral genomes for gene repair events...
Conditions for in vitro maturation and artificial activation of ferret oocytesZiyi Li
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Biol Reprod 66:1380-6. 2002..3 +/- 1.1; n = 11) and trophectoderm (60.8 +/- 2.9, n = 11) cells. These results have begun to elucidate parameters important for animal modeling and cloning with ferrets...
