Research Topics
| Sanford H LeubaSummaryAffiliation: University of Pittsburgh Country: USA Publications
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Detail Information
Publications
Single-molecule analysis of chromatinSanford H Leuba
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Hillman Cancer Center, UPCI Research Pavilion, Pennsylvania 15213-1863, USA
Methods Enzymol 376:73-105. 2004- Expedient placement of two fluorescent dyes for investigating dynamic DNA protein interactions in real timeSanford H Leuba
Department of Cell Biology, University of Pittsburgh School of Medicine and Swanson School of Engineering, Petersen Institute of NanoScience and Engineering and University of Pittsburgh Cancer Institute, Pittsburgh, PA, 15213, USA
Chromosome Res 16:451-67. 2008..Thus, appropriately placed fluorescent dyes can be used to monitor conformational changes occurring in DNA and or proteins and provide increased sensitivity for investigating dynamic DNA-protein interactions in real time... - Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers studySanford H Leuba
Department of Cell Biology and Physiology, School of Medicine, Hillman Cancer Center, University of Pittsburgh Cancer Institute Research Pavilion, Pittsburgh, PA 15213, USA
Proc Natl Acad Sci U S A 100:495-500. 2003..This result is a clear demonstration of the dynamic equilibrium between nucleosome assembly and disassembly at the single chromatin fiber level... - Single-molecule studies of chromatin fibers: a personal reportSanford H Leuba
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Hillman Cancer Center, Pittsburgh, PA 15213, USA
Arch Histol Cytol 65:391-403. 2002..We believe that our results so far have contributed significantly to our understanding of chromatin, but we also hope that this is only the beginning, and that more exciting times lie ahead... - DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchangeSyam P Anand
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
J Bacteriol 189:4502-9. 2007..Further, our results show that the helicase activity of PcrA, although not essential, might play a facilitatory role in the RecA displacement reaction... - Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flowSanford H Leuba
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Methods 47:214-22. 2009..Finally, we present some past successful examples of using these approaches to elucidate unique information about protein-nucleic acid interactions... - Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by single-pair fluorescence resonance energy transferMiroslav Tomschik
Department of Cell Biology and Physiology, Hillman Cancer Center, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Proc Natl Acad Sci U S A 102:3278-83. 2005..The brief excursions into an extended open state may create windows of opportunity for protein factors involved in DNA transactions to bind to or translocate along the DNA... - Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactionsHaocheng Zheng
Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Methods 41:342-52. 2007..We also discuss data processing methods for SCFM... - Steric exclusion and wrapping of the excluded DNA strand occurs along discrete external binding paths during MCM helicase unwindingBrian W Graham
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
Nucleic Acids Res 39:6585-95. 2011..The novel wrapping mechanism stabilizes the MCM complex in a positive unwinding mode, protects the displaced single-stranded DNA tail and prevents reannealing... - Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modificationsPEDRO RODRIGUEZ-COLLAZO
Department of Cell Biology and Physiology, Hillman Cancer Center, University of Pittsburgh, PA 15213, USA
Nucleic Acids Res 37:e81. 2009.... - Dynamics of individual polymers using microfluidic based microcurvilinear flowChao Min Cheng
Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, Scaife Hall, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA
Lab Chip 9:2339-47. 2009.... - Optimal practices for surface-tethered single molecule total internal reflection fluorescence resonance energy transfer analysisMatt V Fagerburg
Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Methods Mol Biol 749:273-89. 2011..Here, we describe some best practices for ensuring that smFRET data are of the highest quality. In addition to instrumentation, we describe sample preparation and data analysis... - Single-molecule approaches reveal the idiosyncrasies of RNA polymerasesJordanka Zlatanova
Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA
Structure 14:953-66. 2006..This review discusses methods and results from this emerging field, and it puts them in perspective of existing biochemical and structural data... - Magnetic tweezers: a sensitive tool to study DNA and chromatin at the single-molecule levelJordanka Zlatanova
Department of Chemistry and Chemicl Engineering, Polytechnic University, Brooklyn, New York 11201, USA
Biochem Cell Biol 81:151-9. 2003..Its potential for chromatin studies is also enormous, and we will briefly present our first chromatin results... - Chromatin fibers, one-at-a-timeJordanka Zlatanova
Department of Chemistry and Chemical Engineering, Polytechnic University, 6 Metro Tech Center, Brooklyn, NY 11201, USA
J Mol Biol 331:1-19. 2003..The beginning is exciting and promising, but much more effort will be needed to take advantage of the huge potential that the new physics-based techniques offer... - Stretching and imaging single DNA molecules and chromatinJordanka Zlatanova
Department of Chemistry and Chemical Engineering, Polytechnic University, Brooklyn, NY 11201, USA
J Muscle Res Cell Motil 23:377-95. 2002.... - Selective requirements for histone H3 and H4 N termini in p300-dependent transcriptional activation from chromatinWoojin An
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
Mol Cell 9:811-21. 2002....