David Knecht

Summary

Affiliation: University of Connecticut
Country: USA

Publications

  1. ncbi Metallothionein mediates leukocyte chemotaxis
    Xiuyun Yin
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    BMC Immunol 6:21. 2005
  2. ncbi Actin binding domains direct actin-binding proteins to different cytoskeletal locations
    Raymond W Washington
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    BMC Cell Biol 9:10. 2008
  3. ncbi Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics
    David A Knecht
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA
    PLoS ONE 5:e14039. 2010
  4. ncbi Traction force microscopy in Dictyostelium reveals distinct roles for myosin II motor and actin-crosslinking activity in polarized cell movement
    Maria L Lombardi
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    J Cell Sci 120:1624-34. 2007
  5. ncbi Automated real-time measurements of leukocyte chemotaxis
    Nacima Hadjout
    Department of Molecular and Cell Biology, The University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269 3125, USA
    J Immunol Methods 320:70-80. 2007
  6. ncbi RacB regulates cytoskeletal function in Dictyostelium spp
    Eunkyung Lee
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Eukaryot Cell 2:474-85. 2003
  7. ncbi Visualization of actin dynamics during macropinocytosis and exocytosis
    Eunkyung Lee
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    Traffic 3:186-92. 2002
  8. ncbi Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis
    Cameron C Scott
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Cell Biol 169:139-49. 2005
  9. ncbi Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcgammaR, phagocytosis
    Isabel M Olazabal
    School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, Birmingham, United Kingdom
    Curr Biol 12:1413-18. 2002
  10. ncbi tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis
    Linnan Tang
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genes Dev 22:2278-90. 2008

Research Grants

  1. MOLECULAR GENETIC ANALYSIS OF THE CYTOSKELETON
    David Knecht; Fiscal Year: 2001
  2. MOLECULAR GENETICS OF DEVELOPMENT IN DICTYOSTELIUM
    David Knecht; Fiscal Year: 1991
  3. Molecular Genetics of the Cytoskeleton
    David Knecht; Fiscal Year: 2009

Collaborators

  • Michael A Lynes
  • Xiuyun Yin
  • Gabriel Fenteany
  • Eunkyung Lee
  • Raymond W Washington
  • Linnan Tang
  • Maria L Lombardi
  • Nacima Hadjout
  • Cameron C Scott
  • Isabel M Olazabal
  • Yu Long
  • Ross M Bickford
  • Mei Yu Chen
  • Yuan Xiong
  • Mei-Yu Chen
  • Pablo A Iglesias
  • Jonathan Franca-Koh
  • Peter N Devreotes
  • Juliet Lee
  • Micah Dembo
  • Philippe Chavrier
  • Wendy Dobson
  • Roberto J Botelho
  • Natasha Coady-Osberg
  • Colin Heath
  • Sergio Grinstein
  • Philip Stahl
  • David J Seastone
  • Ed Harris
  • James A Cardelli
  • Robin C May
  • Laura M Machesky
  • Emmanuelle Caron
  • Kerstin Schilling

Detail Information

Publications10

  1. ncbi Metallothionein mediates leukocyte chemotaxis
    Xiuyun Yin
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    BMC Immunol 6:21. 2005
    ..We have noticed that the MT genes map close to the chemokines Ccl17 and Cx3cl1. Cysteine motifs that characterize these chemokines are also found in the MT sequence suggesting that MT might also act as a chemotactic factor...
  2. ncbi Actin binding domains direct actin-binding proteins to different cytoskeletal locations
    Raymond W Washington
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    BMC Cell Biol 9:10. 2008
    ..Although these two proteins have been extensively characterized, little is known about what regulates their binding to F-actin filaments in the cell...
  3. ncbi Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics
    David A Knecht
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA
    PLoS ONE 5:e14039. 2010
    ..They are also known to cause changes in the organization of the actin cytoskeleton...
  4. ncbi Traction force microscopy in Dictyostelium reveals distinct roles for myosin II motor and actin-crosslinking activity in polarized cell movement
    Maria L Lombardi
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    J Cell Sci 120:1624-34. 2007
    ..We propose that myosin II motor activity increases the rate and frequency at which traction stress asymmetry develops, whereas actin crosslinking activity is important for stabilizing it...
  5. ncbi Automated real-time measurements of leukocyte chemotaxis
    Nacima Hadjout
    Department of Molecular and Cell Biology, The University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269 3125, USA
    J Immunol Methods 320:70-80. 2007
    ..The ECIS/taxis assay system can be used to compare the movement of different cell types, to assess the effect of complex chemotactic gradients, or to determine the effects of pharmaceuticals on chemotactic motility...
  6. ncbi RacB regulates cytoskeletal function in Dictyostelium spp
    Eunkyung Lee
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Eukaryot Cell 2:474-85. 2003
    ....
  7. ncbi Visualization of actin dynamics during macropinocytosis and exocytosis
    Eunkyung Lee
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    Traffic 3:186-92. 2002
    ..These results show that dynamic changes in actin filament association with the vesicle membrane accompany both endocytosis and exocytosis...
  8. ncbi Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis
    Cameron C Scott
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Cell Biol 169:139-49. 2005
    ..These observations suggest that hydrolysis of PI(4,5)P(2) dictates the remodeling of actin necessary for completion of phagocytosis...
  9. ncbi Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcgammaR, phagocytosis
    Isabel M Olazabal
    School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, Birmingham, United Kingdom
    Curr Biol 12:1413-18. 2002
    ..While myosins have been previously implicated in FcgammaR phagocytosis, to our knowledge, this is the first demonstration of a role for myosin-II in CR phagocytosis...
  10. ncbi tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis
    Linnan Tang
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genes Dev 22:2278-90. 2008
    ..Thus, localization of kinase activity to microtubules is critical for TsuA function. We propose that functions in association with the microtubule network may underlie the divergent roles of Fused kinase proteins in different organisms...

Research Grants21

  1. MOLECULAR GENETIC ANALYSIS OF THE CYTOSKELETON
    David Knecht; Fiscal Year: 2001
    ..Several novel in vitro and in vivo assays will be used to assess the consequences of these alterations in the cytoskeleton. ..
  2. MOLECULAR GENETICS OF DEVELOPMENT IN DICTYOSTELIUM
    David Knecht; Fiscal Year: 1991
    ..The purpose of this project is not simply to clone more genes to study their regulation. Rather it is to use functional criteria to isolate important genes as a means to eventually studying the function of the gene products...
  3. Molecular Genetics of the Cytoskeleton
    David Knecht; Fiscal Year: 2009
    ..The structure of the actin binding domains of each of these proteins will also be determined to investigate the mechanism by which they differentially recognize actin filaments. ..