Research Topics
| S B KleiboekerSummaryAffiliation: University of Missouri Country: USA Publications
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Detail Information
Publications
Pathogenesis of African swine fever virus in Ornithodoros ticksS B Kleiboeker
Department of Veterinary Pathobiology, University of Missouri, College of Veterinary Medicine, Columbia 65211, USA
Anim Health Res Rev 2:121-8. 2001..In addition to O. porcinus porcinus, a number of North American, Central American and Caribbean species of Ornithodoros have been shown to be potential vectors of ASFV...
Applications of competitor RNA in diagnostic reverse transcription-PCRSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory and Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211, USA
J Clin Microbiol 41:2055-61. 2003..When inhibition was detected, either dilution of the sample or RNA extraction by an alternative protocol proved successful in eliminating the source of inhibition...
Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCRSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory and Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211, USA
Virol J 2:29. 2005..25 U UNG per 25 microl reaction with a 20 min, 30 degrees C incubation prior to RT-PCR. Under these conditions, high concentrations of amplicon DNA could be degraded while the CT for RNA detection was increased by 1.2 cycles...
Swine fever: classical swine fever and African swine feverSteven B Kleiboeker
Department of Veterinary Pathobiology, Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 1600 East Rollins, Columbia, MO 65211, USA
Vet Clin North Am Food Anim Pract 18:431-51. 2002..oie.int) offer updated information on current worldwide epizootics of ASF and CSF and other swine diseases. Details of biosecurity procedures can be found at http://www.agebb.missouri.edu; see publication G2340...
Detection and multigenic characterization of a herpesvirus associated with malignant catarrhal fever in white-tailed deer (Odocoileus virginianus) from MissouriSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory and Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211, USA
J Clin Microbiol 40:1311-8. 2002....
Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCRSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory and the Department of Veterinary Pathobiology, University of Missouri, College of Veterinary Medicine, Columbia, MO 65211, USA
J Vet Diagn Invest 17:165-70. 2005..The diagnostic sensitivity and specificity of the multiplex reaction was found to be at a minimum equivalent to that of both nested RT-PCR and virus isolation...
Diagnosis of West Nile virus infection in horsesSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
J Vet Diagn Invest 16:2-10. 2004....
Timing of seroconversion and acquisition of positive polymerase chain reaction assay results in calves experimentally infected with bovine leukemia virusDusty W Nagy
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
Am J Vet Res 68:72-5. 2007..To determine the interval to provirus and serum antibody detection (via PCR assay and ELISA, respectively) in calves after experimental inoculation with bovine leukemia virus (BLV)...
Use of a polymerase chain reaction assay to detect bovine leukosis virus in dairy cattleDusty W Nagy
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA
J Am Vet Med Assoc 222:983-5. 2003..Sensitivity and negative predictive value for PCR assay were low. Consequently, PCR assay alone is unreliable for routine detection of BLV in herds with high prevalence of the disease...
Association of two newly recognized herpesviruses with interstitial pneumonia in donkeys (Equus asinus)Steven B Kleiboeker
Department of Veterinary Pathobiology, University of Missouri, College of Veterinary Medicine, Columbia 65211, USA
J Vet Diagn Invest 14:273-80. 2002....
Detection and nucleotide sequencing of a DNA-packaging protein gene of equine gammaherpesvirusesSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory, Department of Veterinary Pathobiology, University of Missouri, College of Veterinary Medicine, Columbia, MO 65211, USA
J Vet Diagn Invest 16:67-74. 2004..Because the role of gammaherpesviruses has not been well defined in equine disease, it is envisioned that a single, sensitive PCR assay to detect these potential pathogens will facilitate further assessment of their role in disease...
West Nile virus encephalitis: sequential histopathological and immunological events in a murine model of infectionDavid Garcia-Tapia
Department of Veterinary Pathobiology Department, University of Missouri Columbia, Columbia, Missouri 65211, USA
J Neurovirol 13:130-8. 2007..These results suggest that the chemokines MCP-5, IP-10, and MIG are important triggers of inflammation in brain due to their early up-regulation following WNV infection...
Retention of ingested porcine reproductive and respiratory syndrome virus in housefliesJennifer A Schurrer
Swine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA
Am J Vet Res 66:1517-25. 2005..Therefore, retention of PRRSV in houseflies appears to be a function of initial virus load after ingestion and environmental temperature. These factors may impact the risk of insect-borne spread of PRRSV among farms...
Decreased periparturient transmission of bovine leukosis virus in colostrum-fed calvesDusty W Nagy
Departments of Veterinary Medicine and Surgery, University of Missouri Columbia, Columbia, MO 65211, USA
J Vet Intern Med 21:1104-7. 2007..Two conclusions have been drawn from previous studies. One suggests that colostrum ingestion transmits BLV to neonatal calves. The second suggests that colostral antibodies are protective...
Detection of equine herpesvirus 3 in equine skin lesions by polymerase chain reactionSteven B Kleiboeker
Veterinary Medical Diagnostic Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
J Vet Diagn Invest 16:74-9. 2004..Using the novel EHV3 nucleotide sequence identified in this report, a sensitive and specific PCR assay targeted to the highly conserved DNA polymerase gene was developed...
Association between the strength of serologic recognition of bovine leukosis virus and lymphocyte count in bovine leukosis virus-infected cowsDusty W Nagy
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61802, USA
J Am Vet Med Assoc 220:1681-4. 2002....
West Nile virus encephalomyelitis in a sheepJeff W Tyler
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
J Vet Intern Med 17:242-4. 2003
Lead enters Rcho-1 trophoblastic cells by calcium transport mechanisms and complexes with cytosolic calcium-binding proteinsTim J Evans
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
Toxicol Appl Pharmacol 186:77-89. 2003..We conclude that trophoblasts have the ability to clear Pb(2+) from the maternal circulation and deliver it to the fetus...
Evaluation of shedding of bovine herpesvirus 1, bovine viral diarrhea virus 1, and bovine viral diarrhea virus 2 after vaccination of calves with a multivalent modified-live virus vaccineSteven B Kleiboeker
Department of Veterinary Pathobiology and the Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
J Am Vet Med Assoc 222:1399-403. 2003..CONCLUSIONS AND CLINICAL RELEVANCE: Shedding of BHV1, BVDV1, and BVDV2 after vaccination was either nonexistent or undetected and did not result in transmission of BHV1, BVDV1, or BVDV2 vaccine viruses to pregnant contact control cows...
Phylogenetic characterization of canine distemper viruses detected in naturally infected dogs in North AmericaIngrid D R Pardo
Veterinary Medical Diagnostic Laboratory, Department of Veterinary Pathobiology, University of Missouri, 1600 E. Rollins, Columbia, MO 65211, USA
J Clin Microbiol 43:5009-17. 2005..Importantly, the three CDV strains detected were demonstrated to be genetically distinct from known vaccine strains and strains previously reported in the continental United States...
Use of a PRRSV infectious clone to evaluate in vitro quasispecies evolutionSusan K Schommer
University of Missouri, Columbia 65211, USA
Adv Exp Med Biol 581:435-8. 2006
Porcine reproductive and respiratory syndrome virus field isolates differ in in vitro interferon phenotypesSang-Myeong Lee
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, 1600 E. Rollins, Columbia, MO 65211, USA
Vet Immunol Immunopathol 102:217-31. 2004..Furthermore, a PRRSV field isolate strongly enhance polyI:C-induced IFN-alpha production in PAM cultures and this priming effect was suppressed by other PRRSV isolates...
Porcine arterivirus activates the NF-kappaB pathway through IkappaB degradationSang-Myeong Lee
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri-Columbia, MO 65211, USA
Virology 342:47-59. 2005..The results presented here provide a basis for understanding molecular pathways of pathology and immune evasion associated with disease caused by PRRSV...
Effect of dietary lipoic acid on metabolic hormones and acute-phase proteins during challenge with infectious bovine rhinotracheitis virus in cattleTy B Schmidt
Division of Animal Science, University of Missouri, MO 65211, USA
Am J Vet Res 67:1192-8. 2006..CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that LA supplementation augmented certain aspects of the immune response; LA32 steers had a more rapid recovery from IBRV viral challenge than did others...
Replication of West Nile virus in equine peripheral blood mononuclear cellsDavid Garcia-Tapia
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
Vet Immunol Immunopathol 110:229-44. 2006..Furthermore, primary equine immune cell cultures represent a potentially useful model of a natural WNV host when testing compounds such as antivirals for use in WNV treatment...
Porcine reproductive and respiratory syndrome virus induces apoptosis through a mitochondria-mediated pathwaySang Myeong Lee
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri Columbia, USA
Virology 365:419-34. 2007..In summary, these findings demonstrate mechanisms by which PRRSV induces apoptosis and will contribute to an enhanced understanding of PRRSV pathogenesis...
Effects of delayed or prolonged fixation on immunohistochemical detection of bovine viral diarrhea virus type I in skin of two persistently infected calvesMargaret A Miller
Department of Veterinary Pathobiology, Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907, USA
J Vet Diagn Invest 17:461-3. 2005..Samples can be held in formalin at least 36 days, without loss of reactivity. A 1-day delay in fixation caused no loss of reactivity, provided the specimen was refrigerated and protected from desiccation...
