Research Topics
Genomes and Genes | Chun Ming HuangSummaryAffiliation: University of California Country: USA Publications
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Publications
Surfactant sodium lauryl sulfate enhances skin vaccination: molecular characterization via a novel technique using ultrafiltration capillaries and mass spectrometric proteomicsChun Ming Huang
Department of Dermatology, Skin Diseases Research Center, University of Alabama, Birmingham, Alabama 35294, USA
Mol Cell Proteomics 5:523-32. 2006..Two secretory proteins, named as calcium-binding protein S100A9 and thymosin beta4, were identified by this novel technique. These two proteins thus may provide new insight into the enhancing effect of surfactants on skin vaccination...- Eradication of drug resistant Staphylococcus aureus by liposomal oleic acidsChun Ming Huang
Division of Dermatology, University of California, San Diego, La Jolla, CA 92093, USA
Biomaterials 32:214-21. 2011..These findings suggest that LipoOA hold great potential to become a new, effective, and safe antimicrobial agent for the treatment of MRSA infections... - In vivo tumor secretion probing via ultrafiltration and tissue chamber: implication for anti-cancer drugs targeting secretomeChun Ming Huang
Division of Dermatology, Department of Medicine, University of California, San Diego, CA 92161, USA
Recent Pat Anticancer Drug Discov 3:48-54. 2008..Many detection methods have been patented regarding probes and peptide used for identification of tumors... - A peptide with a ProGln C terminus in the human saliva peptidome exerts bactericidal activity against Propionibacterium acnesChun Ming Huang
Division of Dermatology, Department of Medicine, University of California, San Diego, and VA San Diego Healthcare Center, Rm 3217A, 3350 La Jolla Village Drive, San Diego, CA, USA
Antimicrob Agents Chemother 52:1834-6. 2008..A GPPPQGGRPQ peptide binds gram-positive Propionibacterium acnes and considerably inhibits bacterial growth. The peptide exhibiting innate immunity may be applied for treatment of various P. acnes-associated human diseases... - In vivo secretome sampling technology for proteomicsChun Ming Huang
Division of Dermatology, Department of Medicine, San Diego, CA, USA Moores Cancer Center, University of California, San Diego, CA, USA La Jolla Institute for Molecular Medicine, San Diego, CA, USA VA San Diego Healthcare Center, San Diego, CA, USA
Proteomics Clin Appl 1:953-62. 2007..Many prospective properties such as simple manipulation, dynamic sampling, pathogen clearance, online mass spectrometric coupling, and disease treatment and monitoring have made CUF probes attractive for clinical uses in the near future... - Mass spectrometric proteomics profiles of in vivo tumor secretomes: capillary ultrafiltration sampling of regressive tumor massesChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA
Proteomics 6:6107-16. 2006..The technique involving CUF probes linked to mass spectrometric proteomics reinforces systems biology studies of cell-cell interactions and is potentially applicable to the discovery of in vivo biomarkers in human disease... - Topical vaccination: the skin as a unique portal to adaptive immune responsesChun Ming Huang
Division of Dermatology, Department of Medicine, University of California, San Diego and VA San Diego Healthcare Center, Rm 3217A, 3350 La Jolla Village Drive, San Diego, CA, USA
Semin Immunopathol 29:71-80. 2007..A recent study demonstrating enhanced skin vaccination by using surfactants to extract partial lamellar lipids of the stratum corneum will also be introduced in this review... - Profiling human saliva endogenous peptidome via a high throughput MALDI-TOF-TOF mass spectrometryChun Ming Huang
Division of Dermatology, Department of Medicine, University of California, San Diego, CA 92161, USA
Comb Chem High Throughput Screen 12:521-31. 2009..Thus, a saliva peptidome containing peptides with -PQ C-termini, as presented here, may reinforce the development of innate-immunity-related disease monitoring using non-invasive saliva samples and mass spectrometry-based techniques... - Quantitative proteomes and in vivo secretomes of progressive and regressive UV-induced fibrosarcoma tumor cells: mimicking tumor microenvironment using a dermis-based cell-trapped system linked to tissue chamberYang Shi
Burnham Institute for Medical Research, La Jolla, CA, USA
Proteomics 7:4589-600. 2007..Differential tumor proteomes and in vivo secretome were thus accentuated as potential therapeutic targets to control tumor growth... - Comparative proteomic profiling of murine skinChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, USA
J Invest Dermatol 121:51-64. 2003..These data establish numerous reference markers within the proteome map of murine skin and provide an important framework for future efforts aimed at characterization of the epidermal and subepidermal responses to environmental changes... - Vaccination targeting a surface sialidase of P. acnes: implication for new treatment of acne vulgarisTeruaki Nakatsuji
Division of Dermatology, Department of Medicine, University of California San Diego, San Diego, California, USA
PLoS ONE 3:e1551. 2008..Despite substantial research into potential new therapies for this common disease, vaccines against acne vulgaris are not yet available... - A differential proteome in tumors suppressed by an adenovirus-based skin patch vaccine encoding human carcinoembryonic antigenChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, VH 501, 1670 University Blvd, Birmingham, AL 35294 0019, USA
Proteomics 5:1013-23. 2005..These proteins may provide a vital link between early-stage tumor suppression and immune response of skin patch vaccination... - Sebum free fatty acids enhance the innate immune defense of human sebocytes by upregulating beta-defensin-2 expressionTeruaki Nakatsuji
Division of Dermatology, Department of Medicine, University of California, San Diego, San Diego, California 92161, USA
J Invest Dermatol 130:985-94. 2010..acnes and enhance the skin's innate antibacterial defense by inducing the expression of hBD-2 in sebocytes as well... - Vaccination targeting surface FomA of Fusobacterium nucleatum against bacterial co-aggregation: Implication for treatment of periodontal infection and halitosisPei Feng Liu
Department of Medicine, Division of Dermatology, University of California, San Diego, CA 92161, USA
Vaccine 28:3496-505. 2010..nucleatum co-opts P. gingivalis to exacerbate gum infections. FomA is highlighted as a potential target for development of new therapeutics against periodontal infection and halitosis in humans... - Bioengineering a humanized acne microenvironment model: proteomics analysis of host responses to Propionibacterium acnes infection in vivoTeruaki Nakatsuji
Department of Medicine, Division of Dermatology, University of California, San Diego, CA, USA
Proteomics 8:3406-15. 2008..The bioengineered acne model thus provides an in vivo microenvironment to study the interaction of host with P. acnes and offers a unique set-up for screening novel anti-acne drugs and vaccines... - Identification of Bacillus anthracis proteins associated with germination and early outgrowth by proteomic profiling of anthrax sporesChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, 35294 0019, USA
Proteomics 4:2653-61. 2004..The 31 new proteins identified in this study also complement an emerging proteomic database of B. anthracis... - In vivo protein sampling using capillary ultrafiltration semi-permeable hollow fiber and protein identification via mass spectrometry-based proteomicsChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, VH 566A, 1670 University Blvd, Birmingham, AL 35294 0019, USA
J Chromatogr A 1109:144-51. 2006..Also, the technique represents a powerful method for the identification of in vivo secreted proteins and has potential application for in the detection of biomarkers for human diseases... - The antimicrobial activity of liposomal lauric acids against Propionibacterium acnesDarren Yang
Department of NanoEngineering, University of California, San Diego, La Jolla, CA 92093, USA
Biomaterials 30:6035-40. 2009..acnes associated diseases... - Staphylococcus aureus hijacks a skin commensal to intensify its virulence: immunization targeting ?-hemolysin and CAMP factorChih Wei Lo
Division of Dermatology, Department of Medicine, University of California, San Diego, San Diego, California 92121, USA
J Invest Dermatol 131:401-9. 2011..acnes and S. aureus. These observations suggest a previously unreported immunotherapy targeting the interaction of S. aureus with a skin commensal... - Vaccine therapy for P. acnes-associated diseasesTeruaki Nakatsuji
Department of Medicine, Division of Dermatology, University of California, San Diego, CA 92161, USA
Infect Disord Drug Targets 8:160-5. 2008..acnes challenge and decreases P. acnes-induced elevation of cytokine production. This review highlights the potential functions of killed P. acnes- and sialidase-based vaccines as novel treatments for P. acnes-associated diseases... - Propionibacterium acnes CAMP factor and host acid sphingomyelinase contribute to bacterial virulence: potential targets for inflammatory acne treatmentTeruaki Nakatsuji
Division of Dermatology, Department of Medicine, University of California San Diego, San Diego, California, United States of America
PLoS ONE 6:e14797. 2011..In the progression of acne vulgaris, the disruption of follicular epithelia by an over-growth of Propionibacterium acnes (P. acnes) permits the bacteria to spread and become in contact with various skin and immune cells... - Proteomics reveals that proteins expressed during the early stage of Bacillus anthracis infection are potential targets for the development of vaccines and drugsChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, Alabama, USA
Genomics Proteomics Bioinformatics 2:143-51. 2004.... - Antibodies elicited by inactivated propionibacterium acnes-based vaccines exert protective immunity and attenuate the IL-8 production in human sebocytes: relevance to therapy for acne vulgarisTeruaki Nakatsuji
Division of Dermatology, Department of Medicine, University of California, San Diego, California 92161, USA
J Invest Dermatol 128:2451-7. 2008..These observations highlight the concept that development of vaccines targeting microbial products may represent an alternative strategy to conventional antibiotic therapy... - Passive immunoprotection targeting a secreted CAMP factor of Propionibacterium acnes as a novel immunotherapeutic for acne vulgarisPei Feng Liu
Department of Medicine, Division of Dermatology, University of California, San Diego, San Diego, CA 92121, USA
Vaccine 29:3230-8. 2011..acnes-associated diseases and highlight the concept of neutralizing P. acnes virulence without disturbing the bacterial commensalism in human microbiome... - In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomicsChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA
Proteomics 6:5805-14. 2006..The technique of using CUF probes linked to mass spectrometric proteomics represents a powerful method to identify in vivo secreted proteins, and may be applicable for identification of proteins relevant in various human diseases... - A novel immunogenic spore coat-associated protein in Bacillus anthracis: characterization via proteomics approaches and a vector-based vaccine systemYu Tsueng Liu
Moores Cancer Center, University of California, San Diego, CA, USA
Protein Expr Purif 57:72-80. 2008..More importantly, the immunogenic SCAP may potentially be a new candidate for the development of anthrax vaccines... - The essentiality of alpha-2-macroglobulin in human salivary innate immunity against new H1N1 swine origin influenza A virusChao Hsuan Chen
Division of Dermatology, Department of Medicine, University of California, San Diego, CA 92121, USA
Proteomics 10:2396-401. 2010..We emphasize that endogenous A2M in human innate immunity functions as a natural inhibitor against S-OIV... - Proteomic characterization of skin and epidermis in response to environmental agentsChun Ming Huang
Department of Dermatology, Skin Diseases Research Center, University of Alabama, Birmingham, AL 35294 0019, USA
Expert Rev Proteomics 2:809-20. 2005.... - Prospective highlights of functional skin proteomicsChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Mass Spectrom Rev 24:647-60. 2005..This type of research into functional skin proteomics will provide a critical step toward understanding disease and developing successful therapeutic strategies... - An innate bactericidal oleic acid effective against skin infection of methicillin-resistant Staphylococcus aureus: a therapy concordant with evolutionary medicineChao Hsuan Chen
Division of Dermatology, Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0671, USA
J Microbiol Biotechnol 21:391-9. 2011..In conclusion, our results suggest a promising therapeutic approach against MRSA through boosting the bacteriocidal activities of native FFAs, which may have been co-evolved during the interactions between microbes and their hosts... - Histone H4 is a major component of the antimicrobial action of human sebocytesDong Youn Lee
Division of Dermatology, University of California San Diego and VA San Diego Healthcare Center, San Diego, California, USA
J Invest Dermatol 129:2489-96. 2009..Taken together, these results indicate that the release of histone H4 by holocrine secretion from the sebaceous gland may play an important role in innate immunity... - Use of nanoparticles as therapy for methicillin-resistant Staphylococcus aureus infectionsPei Feng Liu
Department of Medicine, University of California, San Diego, 92161, USA
Curr Drug Metab 10:875-84. 2009..This review emphasized the potential of nanoparticles in the targeted antibiotics for therapy of staphylococcal infections... - Proteomics integrated with Escherichia coli vector-based vaccines and antigen microarrays reveals the immunogenicity of a surface sialidase-like protein of Propionibacterium acnesCheng Po Huang
Moores Cancer Center, University of California, San Diego, CA, USA Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taiwan
Proteomics Clin Appl 2:1234-45. 2008..In addition, the novel immunogenic SLP potentially serves as an antigen candidate for the development of vaccines targeting P. acnes-associated diseases... - A novel vaccine targeting Fusobacterium nucleatum against abscesses and halitosisPei Feng Liu
Department of Medicine, Division of Dermatology, University of California, San Diego, CA 92161, USA
Vaccine 27:1589-95. 2009..nucleatum. The novel vaccine inducing protective immunity provides an alternative option to conventional antibiotic treatments for chronic halitosis associated with abscesses... - Stimuli-responsive liposome fusion mediated by gold nanoparticlesDissaya Pornpattananangkul
Department of NanoEngineering, University of California at San Diego, La Jolla, California 92093, USA
ACS Nano 4:1935-42. 2010.... - In vivo sampling of extracellular beta-thymosin by ultrafiltration probesYu Tsueng Liu
Moores Cancer Center, University of California, San Diego, CA 92161, USA
Ann N Y Acad Sci 1112:104-13. 2007..The fabrication and biological application of CUF probes are also described. Data from CUF probe-captured beta-thymosin may guide future exploration of extracellular beta-thymosin... - Recent advances in protein profiling of tissues and tissue fluidsShi Yang
The Burnham Institute for Medical Research, Proteomics Facility, La Jolla, CA 92037, USA
Expert Rev Proteomics 4:515-29. 2007..Advances in quantitative proteomics will significantly improve protein profiling of tissue and tissue fluid samples collected by CUF probes... - Antimicrobial property of lauric acid against Propionibacterium acnes: its therapeutic potential for inflammatory acne vulgarisTeruaki Nakatsuji
Division of Dermatology, Department of Medicine, University of California, San Diego, California, USA
J Invest Dermatol 129:2480-8. 2009..acnes-induced ear swelling and granulomatous inflammation. The obtained data highlight the potential of using lauric acid as an alternative treatment for antibiotic therapy of acne vulgaris... - Heat shock proteins HSP27 and HSP70 are present in the skin and are important mediators of allergic contact hypersensitivityNabiha Yusuf
Department of Dermatology and Skin Diseases Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Immunol 182:675-83. 2009..The results suggest that HSPs form a link between adaptive and innate immunity during the early stages of contact hypersensitivity... - Mass spectrometry-based label-free quantitative proteomicsWenhong Zhu
Center on Proteolytic Pathways, Burnham Institute for Medical Research, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
J Biomed Biotechnol 2010:840518. 2010..In this paper, we will discuss the technologies of these label-free quantitative methods, statistics, available computational software, and their applications in complex proteomics studies... - Vaccines and photodynamic therapies for oral microbial-related diseasesPei Feng Liu
Division of Dermatology, Department of Medicine, University of California, San Diego, CA 92161, USA
Curr Drug Metab 10:90-4. 2009..The purpose of this article is to highlight the advantages of vaccine therapy and photodynamic therapy for oral microbial-related diseases compared to treatments with antimicrobial agents and traditional periodontal surgery... - Commensal bacteria regulate Toll-like receptor 3-dependent inflammation after skin injuryYuping Lai
Division of Dermatology, Department of Medicine, University of California San Diego, San Diego, California, USA
Nat Med 15:1377-82. 2009..To our knowledge, these findings show for the first time that the skin epithelium requires TLR3 for normal inflammation after wounding and that the microflora can modulate specific cutaneous inflammatory responses... - Erbium:YAG laser enhances transdermal peptide delivery and skin vaccinationWoan Ruoh Lee
Department of Dermatology, Taipei Medical University Hospital, Taipei, Taiwan
J Control Release 128:200-8. 2008..To reveal the mechanisms underlying these changes, a proteomic technique combined with mass spectrometry was used. This is, to our knowledge, the first report of a laser being used to immunize intact animals... - Proteomics reveals protein profile changes in doxorubicin--treated MCF-7 human breast cancer cellsShui-Tein Chen
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan
Cancer Lett 181:95-107. 2002..Modulation of HSP27 levels may be a clinically useful potential target for design of antitumor drugs and controlling breast tumor growth... - Comparative proteomic analysis of human whole salivaChun Ming Huang
Center for Neuroscience, National Sun Yat Sen University, Kaohsiung, Taiwan
Arch Oral Biol 49:951-62. 2004..The proteomic approach identifies candidates from human whole saliva that may prove to be of diagnostic and therapeutic significance... - A new N-acetylgalactosamine containing peptide as a targeting vehicle for mammalian hepatocytes via asialoglycoprotein receptor endocytosisYing-Ta Wu
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan
Curr Drug Deliv 1:119-27. 2004.... - Proteomic analysis of lipopolysaccharide-induced apoptosis in PC12 cellsYa-Hui Huang
Center for Neuroscience, National Sun Yat-Sen University, Kaohsiung, Taiwan, Republic of China
Proteomics 2:1220-8. 2002..Their identification therefore provides an impetus for further delineation of the cellular and molecular basis of apoptotic cell death and sepsis based on proteomic profiling of PC12 cells... - Decreasing systemic toxicity via transdermal delivery of anticancer drugsJia You Fang
Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan
Curr Drug Metab 9:592-7. 2008..The use of a erbium:YAG (Er:YAG) laser for transdermal delivery of anticancer drugs is specifically highlighted in this review... - Breast tumor microenvironment: proteomics highlights the treatments targeting secretomeShui Tein Chen
Institute of Biological Chemistry and the Genomics Research Center, Academia Sinica, Taipei, Taiwan
J Proteome Res 7:1379-87. 2008..Such in vivo profiling of breast cancer secretomes may facilitate the development of novel drugs specifically targeting secretome...
Research Grants
- Anthrax vaccination by targeting spore germinationChun Ming Huang; Fiscal Year: 2005..This unprecedented study will elicit the pathological mechanisms and vaccination capabilities of immune inhibitor A and camelysin expressed in Bacillus anthracis. ..
- Immunological Secretomes of the Early Anthrax InfectionChun Ming Huang; Fiscal Year: 2010..This protein will thus serve as a positive control to evaluate novel antigens identified in the proposed studies. ..
- Secretomes captured in vivo via ultrafiltration probesChun Ming Huang; Fiscal Year: 2007..We believe that using CUF probes/Mass spectrometry will be a powerful tool which can be applied to other animal models and other human diseases. ..
- Immunological Secretomes of the Early Anthrax InfectionChun Ming Huang; Fiscal Year: 2007..This protein will thus serve as a positive control to evaluate novel antigens identified in the proposed studies. ..