Research TopicsSpecies | Jan HuSummaryAffiliation: University of Michigan Country: USA Publications
Research Grants
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Detail Information
Publications
Cell proliferation and apoptosis in enamelin null miceJan C C Hu
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
Eur J Oral Sci 119:329-37. 2011....- Porcine dentin sialoprotein glycosylation and glycosaminoglycan attachmentsYasuo Yamakoshi
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
BMC Biochem 12:6. 2011..Dentin sialoprotein (Dsp), the N-terminal domain of dentin sialophosphoprotein (Dspp), is a highly glycosylated proteoglycan, but little is known about the number, character, and attachment sites of its carbohydrate moieties... - Enamelin and autosomal-dominant amelogenesis imperfectaJ C C Hu
Department of Orthodontics and Pediatric Dentistry, University of Michigan, School of Dentistry, 1011 North University, Ann Arbor, MI 48109 1078, USA
Crit Rev Oral Biol Med 14:387-98. 2003.... - Proteomic analysis of enamel matrix using a two-dimensional protein fractionation systemYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:266-71; discussion 285-6, 382. 2006..Loss of these glycoproteins is associated with a post-transition increase in enamel mineralization... - How do enamelysin and kallikrein 4 process the 32-kDa enamelin?Yasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:45-51; discussion 93-5, 379-80. 2006..After 12 h of digestion with KLK4, all of the 32-kDa enamelin had been cleaved, but some cleavage products persisted after 48 h of digestion... - A nonsense mutation in the enamelin gene causes local hypoplastic autosomal dominant amelogenesis imperfecta (AIH2)Carina K Mårdh
Department of Medical Genetics Medical Biosciences, Umea University, S 901 87 Umea, Sweden
Hum Mol Genet 11:1069-74. 2002..These findings support ENAM as a disease gene, and shed new light on the molecular mechanism of the disease and to the function of the enamelin protein in enamel formation... - Enamel formation and amelogenesis imperfectaJan C C Hu
University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
Cells Tissues Organs 186:78-85. 2007..In addition, we provide a fresh perspective on the roles matrix proteins play in catalyzing the biomineralization of dental enamel... - Characterization of porcine dentin sialoprotein (DSP) and dentin sialophosphoprotein (DSPP) cDNA clonesYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 111:60-7. 2003.... - Dentin glycoprotein: the protein in the middle of the dentin sialophosphoprotein chimeraYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, Michigan 48108, USA
J Biol Chem 280:17472-9. 2005.... - FAM83H mutations in families with autosomal-dominant hypocalcified amelogenesis imperfectaJung Wook Kim
Department of Cell and Developmental Biology, Dental Research Institute, School of Dentistry, Seoul National University, 275 1 Yongon dong, Chongno Gu, Seoul 110 768, Korea
Am J Hum Genet 82:489-94. 2008..3. The mutations perfectly cosegregate with the disease phenotype and demonstrate that FAM83H is required for proper dental-enamel calcification... - Porcine dentin sialoprotein is a proteoglycan with glycosaminoglycan chains containing chondroitin 6-sulfateYasuo Yamakoshi
University of Michigan Dental Research Laboratory, Ann Arbor, Michigan 48108, USA
J Biol Chem 280:1552-60. 2005..DSP binds biotin-labeled hyaluronic acid, and such binding is inhibited by the addition of unlabeled hyaluronic acid. We conclude that DSP is a proteoglycan and that GAG attachments are the predominant structural feature of porcine DSP... - Dentin sialophosphoprotein is processed by MMP-2 and MMP-20 in vitro and in vivoYasuo Yamakoshi
Departments of Biologic and Materials Sciences, University of Michigan Dental Research Laboratory, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
J Biol Chem 281:38235-43. 2006..quot; Exact correspondence between DSPP cleavage sites that occur in vivo and those generated in vitro demonstrates that MMP-2 and MMP-20 process DSPP into smaller subunits in the dentin matrix during odontogenesis... - Porcine dentin matrix protein 1: gene structure, cDNA sequence, and expression in teethJung Wook Kim
University of Michigan Dental Research Laboratory, Ann Arbor, 48108, USA
Eur J Oral Sci 114:33-41. 2006..In developing teeth, immunohistochemistry localized DMP1 in odontoblasts and the dentinal tubules of mineralized dentin and in ameloblasts, but not in the enamel matrix... - Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in miceJan C C Hu
Department of Orthodontics and Pediatric Dentistry and Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48108, USA
J Biol Chem 283:10858-71. 2008..These results demonstrate ameloblast-specific expression of enamelin and reveal that enamelin is essential for proper enamel matrix organization and mineralization... - Porcine dentin sialophosphoprotein: length polymorphisms, glycosylation, phosphorylation, and stabilityYasuo Yamakoshi
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
J Biol Chem 283:14835-44. 2008..The 4 alleles encode DPP domains having 551, 575, 589, or 594 amino acids and completely explain the DPP size variations. DPP length variations are polymorphic and are not associated with dentin defects... - Proteomics and genetics of dental enamelJan C-C Hu
University of Michigan Dental Research Lab, Ann Arbor, Mich. 48108, USA
Cells Tissues Organs 181:219-31. 2005.... - Porcine SPARC: isolation from dentin, cDNA sequence, and computer modelYong-Hee P Chun
University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA
Eur J Oral Sci 114:78-85; discussion 93-5, 379-80. 2006..SPARC is composed of three structural/functional domains: an acidic Ca2+-binding, a follistatin-like, and an extracellular calcium-binding domain... - Establishment of porcine pulp-derived cell lines and expression of recombinant dentin sialoprotein and recombinant dentin matrix protein-1Takanori Iwata
University of Michigan Dental Research Laboratory, Ann Arbor, MI, USA
Eur J Oral Sci 115:48-56. 2007..We conclude that we have immortalized porcine odontoblast-like cells which express recombinant dentin extracellular matrix components with post-translational modifications that closely resemble those produced in vivo... - Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam)Yuanyuan Hu
Department of Orthodontics and Pediatric Dentistry, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
Eur J Oral Sci 116:113-23. 2008..Our study provides new insights into regulatory mechanisms governing enamelin expression... - Mutational analysis of candidate genes in 24 amelogenesis imperfecta familiesJung-Wook Kim
University of Michigan School of Dentistry, University of Michigan Dental Research Laboratory, Ann Arbor, MI 48108, USA, and Department of Pediatric Dentistry and Dental Research Institute, Seoul, Korea
Eur J Oral Sci 114:3-12; discussion 39-41, 379. 2006..Out of 24 families with isolated enamel defects, only six disease-causing mutations were identified in the AI candidate genes. This finding suggests that many additional genes potentially contribute to the etiology of AI...
Research Grants
- ENAMEL WITHOUT ENAMELINJan Hu; Fiscal Year: 2007..The findings will establish an appropriate knowledge base for framing future hypotheses concerning the molecular mechanisms by which enamelin controls enamel biomineralization. ..
- Why is Fam83h critical for enamel formation?Jan C C Hu; Fiscal Year: 2010..Our long term goal is to advance understanding of normal and pathological tooth formation to stimulate improvements in the public's oral health. ..