Research Topics
Species | Kathryn V HolmesSummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
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Publications
Substitutions of conserved amino acids in the receptor-binding domain of the spike glycoprotein affect utilization of murine CEACAM1a by the murine coronavirus MHV-A59Larissa B Thackray
Department of Microbiology, University of Colorado Health Sciences Center, East 19th Avenue, Aurora, CO 80110, USA
Virology 334:98-110. 2005..However, viruses with Y162F substitutions had wild type growth, suggesting that Y162 may comprise part of a hydrophobic domain that contacts the MHV-binding site of mCEACAM1a...- SARS-associated coronavirusKathryn V Holmes
University of Colorado Health Sciences Center, Denver, USA
N Engl J Med 348:1948-51. 2003 - SARS coronavirus: a new challenge for prevention and therapyKathryn V Holmes
University of Colorado Health Sciences Center, Department of Microbiology, Denver, Colorado 80262, USA
J Clin Invest 111:1605-9. 2003 - Amino acid substitutions and an insertion in the spike glycoprotein extend the host range of the murine coronavirus MHV-A59Larissa B Thackray
Department of Microbiology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262, USA
Virology 324:510-24. 2004..Thus, relatively few changes in the N-terminal region of S1 are sufficient to permit MHV-A59 to interact with alternative receptors on murine and non-murine cells... - Structural biology. Adaptation of SARS coronavirus to humansKathryn V Holmes
University of Colorado Health Sciences Center, Mail Stop 8333, Post Office Box 6211, Aurora, CO 80045, USA
Science 309:1822-3. 2005 - Virology. The SARS coronavirus: a postgenomic eraKathryn V Holmes
Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Science 300:1377-8. 2003 - Host-pathogen interactions during coronavirus infection of primary alveolar epithelial cellsTanya A Miura
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho, USA
J Leukoc Biol 86:1145-51. 2009..Understanding the molecular details of these interactions will be critical for the design of effective strategies to prevent and treat coronavirus infections in the lung... - Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokinesTanya A Miura
Department of Microbiology, University of Colorado Health Sciences Center, MS 8333, PO Box 6511, Aurora, CO 80045, USA
Virology 369:288-98. 2007..Primary cultures of alveolar epithelial cells are an important model for the early events in viral infection that lead to pulmonary inflammation... - Rat coronavirus infection of primary rat alveolar epithelial cellsTanya A Miura
University of Colorado Health Sciences Center, Aurora 80010, USA
Adv Exp Med Biol 581:351-6. 2006 - Human coronavirus 229E: receptor binding domain and neutralization by soluble receptor at 37 degrees CJamie J Breslin
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Virol 77:4435-8. 2003..Soluble hAPN neutralized the infectivity of HCoV-229E virions at 37 degrees C, but not 4 degrees C. Binding of hAPN may therefore trigger conformational changes in the viral spike protein at 37 degrees C that facilitate virus entry... - Template-based coiled-coil antigens elicit neutralizing antibodies to the SARS-coronavirusBrian Tripet
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
J Struct Biol 155:176-94. 2006.... - Mutational analysis of aminopeptidase N, a receptor for several group 1 coronaviruses, identifies key determinants of viral host rangeSonia M Tusell
Molecular Biology Program, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
J Virol 81:1261-73. 2007..Therefore, entry and host range of these group 1 coronaviruses depend on the ability of the viral spike glycoproteins to recognize small, species-specific amino acid differences in the APN proteins of different species... - Dissection of the fusion machine of SARS-coronavirusMegan W Howard
University of Colorado Health Sciences Center, Aurora 80045, USA
Adv Exp Med Biol 581:319-22. 2006 - The N-terminal region of the murine coronavirus spike glycoprotein is associated with the extended host range of viruses from persistently infected murine cellsJeanne H Schickli
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Virol 78:9073-83. 2004..Thus, 21 amino acid substitutions and a 7-amino-acid insert in the N-terminal region of the S glycoprotein of MHV/BHK confer the ability to bind and in some cases infect cells of nonmurine species... - Aromatic amino acids in the juxtamembrane domain of severe acute respiratory syndrome coronavirus spike glycoprotein are important for receptor-dependent virus entry and cell-cell fusionMegan W Howard
Dept of Microbiology, Mail Stop 8333, 12800 East 19th Ave, P O Box 6511, Aurora, CO 80045, USA
J Virol 82:2883-94. 2008..Because the JMD is so highly conserved in all coronavirus S proteins, it is a potential target for development of drugs that may inhibit virus entry and/or cell-cell fusion mediated by S proteins of all coronaviruses... - Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229EAurelio Bonavia
Department of Microbiology Molecular Biology Program, University of Colorado Health Sciences Center, 4200 E 9th Avenue, Denver, CO 80262, USA
J Virol 77:2530-8. 2003..Thus, the data suggest that the domain of the spike protein between amino acids 417 and 547 is required for the binding of HCoV-229E to its hAPN receptor... - Rat respiratory coronavirus infection: replication in airway and alveolar epithelial cells and the innate immune responseC Joel Funk
National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA
J Gen Virol 90:2956-64. 2009.... - Conformational changes in the spike glycoprotein of murine coronavirus are induced at 37 degrees C either by soluble murine CEACAM1 receptors or by pH 8Bruce D Zelus
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA
J Virol 77:830-40. 2003.... - Structural characterization of the SARS-coronavirus spike S fusion protein coreBrian Tripet
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, CO 80262, USA
J Biol Chem 279:20836-49. 2004..Overall, the structure of the hetero-stranded complex is consistent with the structures observed for other type 1 viral fusion proteins in their fusion-competent state... - The N-terminal domain of the murine coronavirus spike glycoprotein determines the CEACAM1 receptor specificity of the virus strainJean C Tsai
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
J Virol 77:841-50. 2003..These data suggest that the RBD domain and the rest of the spike must coevolve to optimize function in viral entry and spread... - Human angiotensin-converting enzyme 2 (ACE2) is a receptor for human respiratory coronavirus NL63M K Smith
University of Colorado Health Sciences Center, Aurora 80045, USA
Adv Exp Med Biol 581:285-8. 2006 - Blinded case-control study of the relationship between human coronavirus NL63 and Kawasaki syndromeSamuel R Dominguez
Department of Pediatrics, The Children's Hospital, University of Colorado Health Science Center, Denver, CO, USA
J Infect Dis 194:1697-701. 2006..These data suggest that, although HCoV-NL63 was circulating in children in our community during the time of the study, the prevalence of infection with HCoV-NL63 was not greater in patients with KS than in control subjects... - The spike glycoprotein of murine coronavirus MHV-JHM mediates receptor-independent infection and spread in the central nervous systems of Ceacam1a-/- MiceTanya A Miura
Department of Microbiology MS 8333, University of Colorado Health Sciences Center, 12800 E 19th Ave, P O Box 6511, Aurora, CO 80045, USA
J Virol 82:755-63. 2008..Thus, the ability of the MHV-JHM spike to mediate mCEACAM1a-independent spread in the brain is likely an important factor in the severe neurovirulence of MHV-JHM in wild-type mice... - Detection of four human coronaviruses in respiratory infections in children: a one-year study in ColoradoSamuel R Dominguez
Department of Pediatrics, The Children s Hospital, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
J Med Virol 81:1597-604. 2009..Our data raise the possibility that HCoV may play a role in gastrointestinal and CNS disease. Additional studies are needed to investigate the potential roles of HCoVs in these diseases... - Detection of group 1 coronaviruses in bats in North AmericaSamuel R Dominguez
University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
Emerg Infect Dis 13:1295-300. 2007..Because of the potential for bat coronaviruses to cause disease in humans and animals, further surveillance and characterization of bat coronaviruses in North America are needed... - CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirusScott A Jeffers
Department of Microbiology and Molecular Biology Program, University Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262, USA
Proc Natl Acad Sci U S A 101:15748-53. 2004..Our data suggest that the large S glycoprotein of SARS-CoV may use both ACE2 and CD209L in virus infection and pathogenesis... - Molecular interactions of group 1 coronaviruses with feline APNSonia M Tusell
University of Colorado Health Sciences Center, Aurora 80045, USA
Adv Exp Med Biol 581:289-91. 2006 - Human coronavirus 229E can use CD209L (L-SIGN) to enter cellsScott A Jeffers
University of Colorado Health Sciences Center at Fitzsimons, Aurora, USA
Adv Exp Med Biol 581:265-9. 2006 - Cells of human aminopeptidase N (CD13) transgenic mice are infected by human coronavirus-229E in vitro, but not in vivoDavid E Wentworth
Department of Microbiology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
Virology 335:185-97. 2005..This hAPN-transgenic line will also be used for crossbreeding experiments with other knockout, immune deficient, or transgenic mice to identify factors, in addition to hAPN, that are required for HCoV-229E infection... - Ceacam1a-/- mice are completely resistant to infection by murine coronavirus mouse hepatitis virus A59Erin Hemmila
Department of Microbiology, University of Colorado Health Sciences Center, Denver, USA
J Virol 78:10156-65. 2004..These results indicate that CEACAM1a is the sole receptor for MHV-A59 in both liver and brain and that its deletion from the mouse renders the mouse completely resistant to infection by this virus... - Receptor-dependent coronavirus infection of dendritic cellsBrian C Turner
Department of Microbiology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262, USA
J Virol 78:5486-90. 2004.... - Global distribution of novel rhinovirus genotypeThomas Briese
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York 10032, USA
Emerg Infect Dis 14:944-7. 2008..Molecular dating indicates that these viruses have been circulating for at least 250 years... - Crystal structure of murine sCEACAM1a[1,4]: a coronavirus receptor in the CEA familyKemin Tan
Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
EMBO J 21:2076-86. 2002..We discuss the structural basis of virus receptor activities of murine CEACAM1 proteins, binding of Neisseria to human CEACAM1, and other homophilic and heterophilic interactions of CEA family members... - SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cellsEric C Mossel
Colorado State University, Fort Collins, CO 80523, USA
Virology 372:127-35. 2008..Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection... - Maneuvering for advantage: the genetics of mouse susceptibility to virus infectionSeung-Hwan Lee
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5
Trends Genet 19:447-57. 2003..We focus especially on the innate mechanisms that function as the host's first line of defense against infection. We also discuss the main issues that confront this field, as well as its future... - Potential for receptor-based antiviral drugs against SARSRichard K Williams
Lancet 362:77. 2003 - Expression of the mouse hepatitis virus receptor by central nervous system microgliaChandran Ramakrishna
Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
J Virol 78:7828-32. 2004....
Research Grants
- MOLECULAR PATHOGENESIS OF INFECTIOUS DISEASESKathryn Holmes; Fiscal Year: 2007....