Research Topics
Species | Brian FoxSummaryAffiliation: University of Wisconsin Country: USA Publications
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Publications
Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombinationSandy Thao
Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA
J Struct Funct Genomics 5:267-76. 2004..Sequence verified entry clones were then used to create expression vectors again via the Gateway system...- Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studiesRobert C Tyler
Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, Wisconsin 53706, USA
Proteins 59:633-43. 2005..coli platform when successful yields more protein, as currently needed for crystallization trials for X-ray structure determination... - Small-scale, semi-automated purification of eukaryotic proteins for structure determinationRonnie O Frederick
The University of Wisconsin Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Room 141B, 433 Babcock Drive, Madison, WI 53706, USA
J Struct Funct Genomics 8:153-66. 2007.... - Structural genomics: from genes to structures with valuable materials and many questions in betweenBrian G Fox
Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
Nat Methods 5:129-32. 2008.... - X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1Brian G Fox
University of Wisconsin Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, 53706, USA
Proteins 61:206-8. 2005 - Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenaseLucas J Bailey
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
Protein Expr Purif 57:9-16. 2008..15 microM and 3.0 s(-1), respectively. This expression system and purification allows for the recovery of the soluble oxidoreductase in yields that facilitate further biochemical and structural characterizations... - Characterization of the nitrosyl adduct of substrate-bound mouse cysteine dioxygenase by electron paramagnetic resonance: electronic structure of the active site and mechanistic implicationsBrad S Pierce
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
Biochemistry 46:8569-78. 2007..Moreover, the computed EPR parameters (g and A values) are in excellent agreement with experimental results for this CDO species and those obtained from comparable synthetic {FeNO}7 (S = 1/2) iron-nitrosyl complexes... - Structures of proteins of biomedical interest from the Center for Eukaryotic Structural GenomicsGeorge N Phillips
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
J Struct Funct Genomics 8:73-84. 2007..Many times proteins qualify in more than one of these categories. Here we review some of the structures that have connections to human health and disease... - Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction mediumHassan K Sreenath
Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
Protein Expr Purif 40:256-67. 2005..These protein preparations have been utilized to solve X-ray crystal structures by multiwavelength anomalous diffraction phasing... - X-ray structure of ILL2, an auxin-conjugate amidohydrolase from Arabidopsis thalianaEduard Bitto
Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
Proteins 74:61-71. 2009..Overall, the structure of ILL2 suggests that this enzyme likely uses a catalytic mechanism that follows the paradigm established for the other enzymes of the M20 peptidase family... - Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphurariaJason G McCoy
Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Proteins 74:368-77. 2009..1 degrees C. Modeling of cofactor binding in the active site based on the structure of methoxy mycolic acid synthase 2 revealed a number of conserved interactions within the active site... - Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determinationRobert C Tyler
Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1549, USA
Protein Expr Purif 40:268-78. 2005..1H-15N heteronuclear single quantum correlation spectroscopy as well as 13C; 15N-edited spectroscopy showed that the purified [U-15N]- and [U-13C, U-15N]-labeled proteins were suitable for structure analysis... - Sulfate requirement for heterotrophic growth of "Ferroplasma acidarmanus" strain fer1David J Baumler
Cellular and Molecular Biology Program, Kaspar Laboratory, University of Wisconsin Madison, 1925 Willow Dr, Madison, WI 53706, USA
Res Microbiol 156:492-8. 2005..Sulfide was absent in the precipitate but zinc and sulfur were detected. These data demonstrate that SO4 is required for heterotrophic growth of strain fer1 and may have a role in the global sulfur cycle... - The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thalianaEduard Bitto
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, USA
Acta Crystallograph Sect F Struct Biol Cryst Commun 61:469-72. 2005..The metal-binding site was defined and is similar to the iron(II) binding sites found in other members of the superfamily... - The Center for Eukaryotic Structural GenomicsJohn L Markley
Center for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin Madison, Madison, WI 53706, USA
J Struct Funct Genomics 10:165-79. 2009.... - Autoinduction of protein expressionBrian G Fox
Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin, USA
Curr Protoc Protein Sci . 2009..Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included... - The length of the bound fatty acid influences the dynamics of the acyl carrier protein and the stability of the thioester bondGregory A Zornetzer
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin53706, USA
Biochemistry 49:470-7. 2010..Thus, acyl-ACP offers an interesting system for future investigations of complex protein motions on the micro- and millisecond time scales... - Structure and mechanism of mouse cysteine dioxygenaseJason G McCoy
Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin, Madison, 53706 1544, USA
Proc Natl Acad Sci U S A 103:3084-9. 2006..Details of the predicted active site are used to present and discuss a plausible mechanism of action for the enzyme... - High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomicsWon Bae Jeon
The Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, 433 Bobcock Drive, 53706-1549, USA
J Struct Funct Genomics 6:143-7. 2005..The protocols have been used successfully to provide high quality proteins that are suitable for structural studies by X-ray crystallography and NMR... - X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3Thomas E Malone
Molecular and Environmental Toxicology Program, University of Wisconsin-Madison, 53706, USA
Proteins 58:243-5. 2005 - Structure of human J-type co-chaperone HscB reveals a tetracysteine metal-binding domainEduard Bitto
Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
J Biol Chem 283:30184-92. 2008..The normal mode analysis of HscB revealed that this L-shaped protein preferentially undergoes a scissors-like motion that correlates well with the conformational changes of human HscB observed in the crystals... - Identification of the binding region of the [2Fe-2S] ferredoxin in stearoyl-acyl carrier protein desaturase: insight into the catalytic complex and mechanism of actionPablo Sobrado
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 45:4848-58. 2006..These results are considered in the light of the presently available models for Fd-mediated electron transfer in Delta9D and other protein-protein complexes... - Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15NQin Zhao
Center for Eukaryotic Structural Genomics and Department of Biochemistry, University of Wisconsin--Madison, 433 Babcock Drive, Madison, WI 53706, USA
J Struct Funct Genomics 5:87-93. 2004.... - Structural and functional characterization of a novel phosphatase from the Arabidopsis thaliana gene locus At1g05000David J Aceti
Department of Biochemistry, The Center for Eukaryotic Structural Genomics, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA
Proteins 73:241-53. 2008..Divalent metal cations were not required for activity and had little effect on the reaction... - A graphical approach to tracking and reporting target status in structural genomicsXiaokang Pan
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
J Struct Funct Genomics 8:209-16. 2007..This software package has facilitated inter-group communication, improved the quality and accuracy of information in our LIMS, and increased the efficiency and accuracy of our reports to PepcDB... - Combined participation of hydroxylase active site residues and effector protein binding in a para to ortho modulation of toluene 4-monooxygenase regiospecificityKevin H Mitchell
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706-1544, USA
Biochemistry 41:3176-88. 2002..Evolutionary and mechanistic implications of these findings are considered... - X-ray structure of a soluble Rieske-type ferredoxin from Mus musculusElena J Levin
Department of Biochemistry, University of Wisconsin, Madison, USA
Acta Crystallogr D Biol Crystallogr 64:933-40. 2008..Based on its high sequence identity, the structure of the human homolog is likely to be modeled well by the mouse Rieske-type ferredoxin structure... - Crystallographic and catalytic studies of the peroxide-shunt reaction in a diiron hydroxylaseLucas J Bailey
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
Biochemistry 48:8932-9. 2009..These results provide a new structural benchmark for how activated intermediates may be formed and dispatched during diiron hydroxylase catalysis... - Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenaseNathaniel L Elsen
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
Biochemistry 48:3838-46. 2009..This combination of catalytic and structural studies offers important new insight into the role of the role of conserved Thr-201, and its contributions to the catalytic reaction cycle... - Spectroscopic and computational characterization of substrate-bound mouse cysteine dioxygenase: nature of the ferrous and ferric cysteine adducts and mechanistic implicationsJessica D Gardner
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Biochemistry 49:6033-41. 2010.... - Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteinsPil Seok Chae
Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
Nat Methods 7:1003-8. 2010..MNG amphiphiles are promising tools for membrane protein science because of the ease with which they may be prepared and the facility with which their structures may be varied... - Fluorescence anisotropy studies of enzyme-substrate complex formation in stearoyl-ACP desaturaseJeffrey A Haas
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 41:14472-81. 2002..These results indicate that acyl chain length selectivity derives in major part from partition of the enzyme-substrate complex between substrate release and subsequent steps in catalysis... - Remarkable aliphatic hydroxylation by the diiron enzyme toluene 4-monooxygenase in reactions with radical or cation diagnostic probes norcarane, 1,1-dimethylcyclopropane, and 1,1-diethylcyclopropaneLuke A Moe
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706-1544, USA
Biochemistry 43:15688-701. 2004..The results show a divergence of radical and cation pathways for T4moH-mediated hydroxylation that can be dissected by diagnostic substrate probe rearrangements and by changes in the source of oxygen used for substrate oxygenation... - Preparation of isotopically labeled spinach acyl-acyl carrier protein for NMR structural studiesGregory A Zornetzer
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1549, USA
Protein Expr Purif 46:446-55. 2006.... - Reactions of the diiron enzyme stearoyl-acyl carrier protein desaturaseBrian G Fox
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
Acc Chem Res 37:421-9. 2004..For soluble Delta9D, the natural reaction may initiate at the C-10 position, in contrast to the well-established initial reactivity of the membrane enzyme homologue stearoyl-coenzyme A (CoA) Delta(9) desaturase at the C-9 position... - Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complexMichael A Goren
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI 53706, USA
Protein Expr Purif 62:171-8. 2008..The applicability of cell-free translation to the assembly and purification of other integral membrane protein complexes is discussed... - Structural consequences of effector protein complex formation in a diiron hydroxylaseLucas J Bailey
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706 1544, USA
Proc Natl Acad Sci U S A 105:19194-8. 2008..This description provides insight into the functional role of effector protein binding in all carboxylate-bridged diiron hydroxylases... - A combined approach to improving large-scale production of tobacco etch virus proteasePaul G Blommel
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Protein Expr Purif 55:53-68. 2007..coli cell paste). Methods for producing glutathione-S-transferase-tagged TEV with similar yields (approximately 12 mg pure protease fusion per gram of E. coli cell paste) are also reported... - Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium compositionPaul G Blommel
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Biotechnol Prog 23:585-98. 2007..Upon the basis of the analysis of over 500 independent expression results, combinations of optimized expression media and expression plasmids that gave protein yields of greater than 1000 mug/mL of expression culture were identified... - Rapid-mix and chemical quench studies of ferredoxin-reduced stearoyl-acyl carrier protein desaturaseKaren S Lyle
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 42:5857-66. 2003..4), indicating C-H bond cleavage does not contribute significantly to the rate-limiting steps of pre-steady-state catalysis. These results were used to assemble and evaluate a minimal kinetic model for Delta9D catalysis... - Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenaseLuke A Moe
Department of Biochemistry, University of Wisconsin-Madison, USA
Acta Crystallogr D Biol Crystallogr 62:476-82. 2006..These structural differences are identified as possible contributors to the evolutionary specialization of soluble Rieske-type ferredoxins between the diiron monooxygenases and cis-dihydrodiol-forming dioxygenases... - Solution structures of spinach acyl carrier protein with decanoate and stearateGregory A Zornetzer
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706-1549, USA
Biochemistry 45:5217-27. 2006..These structures demonstrate the conformational flexibility of spinach ACP and suggest how the protein changes to accommodate its myriad binding partners... - X-ray structure of Danio rerio secretagogin: A hexa-EF-hand calcium sensorEduard Bitto
Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 53706 1544, USA
Proteins 76:477-83. 2009.... - Insight into the mechanism of aromatic hydroxylation by toluene 4-monooxygenase by use of specifically deuterated toluene and p-xyleneKevin H Mitchell
Department of Biochemistry, University of Wisconsin, Madison, WI 53706-1544, USA
Proc Natl Acad Sci U S A 100:3784-9. 2003.... - Desaturation, chain scission, and register-shift of oxygen-substituted fatty acids during reaction with stearoyl-ACP desaturaseCorina E Rogge
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Biochemistry 41:10141-8. 2002..Possible mechanisms of the acyl chain scission and implications of these results for the desaturation mechanism are considered... - Crystal structure of gene locus At3g16990 from Arabidopsis thalianaPaul G Blommel
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Proteins 57:221-2. 2004 - Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobioseAllison Riederer
DOE Great Lakes Bioenergy Research Center, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Appl Environ Microbiol 77:1243-53. 2011..The pattern of expression of transcriptional regulators, sigma factors, and signal transducers indicates that response to growth rate is the dominant global mechanism used for control of gene expression in C. thermocellum... - Crystal structure of the protein from gene At3g17210 of Arabidopsis thalianaCraig A Bingman
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Proteins 57:218-20. 2004 - One-plasmid tunable coexpression for mycobacterial protein-protein interaction studiesYong Chang
Biophysics Graduate Degree Program, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706 1549, USA
Protein Sci 18:2316-25. 2009..Furthermore, the tunable coexpression strategy used herein should also be applicable in other species with minor modifications... - Component interactions and implications for complex formation in the multicomponent toluene 4-monooxygenaseLuke A Moe
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706-1544, USA
Biochemistry 45:5478-85. 2006..The results indicate that specialization of effector protein function may include specific protein-protein interactions with [2Fe-2S] domains as well as the hydroxylase component... - Cell-free translation of integral membrane proteins into unilamelar liposomesMichael A Goren
Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
Methods Enzymol 463:647-73. 2009.... - Flexi vector cloningPaul G Blommel
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
Methods Mol Biol 498:55-73. 2009..The protocol also includes transfer of the sequence-verified clones into another Flexi Vector plasmid backbone. Smaller numbers of cloning reactions can be undertaken by appropriate scaling of the indicated reaction volumes... - X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37RvDavid H Dyer
Department of Biochemistry, 433 Babcock Dr, University of Wisconsin, Madison, Madison, WI 53706, USA
Protein Sci 14:1508-17. 2005..Instead, the amino group of Lys-76 occupied this position. The relationships between DesA2 and known diiron enzymes are discussed... - Cell-free protein synthesis technology in NMR high-throughput structure determinationShin ichi Makino
Department of Biochemistry, Center for Eukaryotic Structural Genomics, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI, USA
Methods Mol Biol 607:127-47. 2010.... - A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesiclesPablo Sobrado
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Room 141B, 433 Babcock Drive, Madison, WI 53706, USA
Protein Expr Purif 58:229-41. 2008..The utility of this approach as a delivery method for production and incorporation of monotopic (peripheral) membrane proteins is discussed... - Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thalianaDavid W Smith
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Proteins 57:854-7. 2004 - Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoaElitza S Sevova
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA
J Biol Chem 285:20580-7. 2010..These results represent the first application of cell-free synthesis for the rapid preparation and functional annotation of integral membrane proteins and thus illustrate its utility in studying otherwise intractable enzyme systems... - High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning systemPaul G Blommel
Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin, Madison, 53706-1549, USA
Protein Expr Purif 47:562-70. 2006..With the proper construction in the flanking sequence of the vector, gene transfer efficiencies of 95-98% were demonstrated... - Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteinsPaul G Blommel
The University of Wisconsin Center for Eukaryotic Structural Genomics, Biophysics Graduate Degree Program and Biochemistry Department, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
Anal Biochem 336:75-86. 2005..The assay was also demonstrated as a general tool for monitoring proteolysis of multidomain fusion proteins containing an internal protease site such as are being created in structural genomics studies worldwide... - Crystallization and preliminary analysis of native and N-terminal truncated isoforms of toluene-4-monooxygenase catalytic effector proteinAllen M Orville
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
Acta Crystallogr D Biol Crystallogr 59:572-5. 2003..These crystallographic results are the first available for the evolutionarily related but functionally diversified catalytic effector proteins from the multicomponent diiron monooxygenase family... - Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector proteinGeorge T Lountos
School of Chemistry and Biochemistry, Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA
Biochemistry 44:7131-42. 2005..The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the approximately 33 residue N-terminal region for catalytic activity... - Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferaseNatasha M Nesbitt
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
Protein Expr Purif 39:269-82. 2005..The specific activity of the purified protein is 0.541 U mg(-1), indicating a turnover number of approximately 0.2 s(-1), and the apparent Km values for octanoyl-ACP and apo-H protein are 10.2+/-4.4 and 13.2+/-2.9 microM, respectively... - Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-BAllen M Orville
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
Acta Crystallogr D Biol Crystallogr 60:957-61. 2004..Thus, the local symmetry produces approximate systematic absences along the (00l) principal axis and complicates the space-group determination... - Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complexLars Skjeldal
IKB, Agricultural University of Norway, Box 5040, 1432 NLH, Norway
J Biol Inorg Chem 9:945-53. 2004..Comparisons are made between the present NMR structure, previous paramagnetic NMR studies of T4moC, and the X-ray structures of other members of the Rieske protein family... - Identification of transcribed sequences in Arabidopsis thaliana by using high-resolution genome tiling arraysViktor Stolc
NASA ARC
Proc Natl Acad Sci U S A 102:4453-8. 2005..Transcription activities were also detected for many of the intergenic regions and the small RNAs, including tRNA, small nuclear RNA, small nucleolar RNA, and microRNA. Expression of tRNAs correlates with genome-wide amino acid usage... - N-isotope effects on the Raman spectra of Fe(2)S(2) ferredoxin and Rieske ferredoxin: evidence for structural rigidity of metal sitesFrederik J Rotsaert
Department of Biochemistry and Molecular Biology, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006-8921, USA
J Biol Inorg Chem 8:318-26. 2003..In both cases, the structural rigidity is likely to play a role in minimizing the reorganization energy for electron transfer... - Reaction mechanisms of non-heme diiron hydroxylases characterized in whole cellsErin Bertrand
Department of Chemistry, Bates College, 5 Andrews Rd. Lewiston, ME 04240, United States
J Inorg Biochem 99:1998-2006. 2005..No radical lifetime dependence on substrate concentration was seen. Results from experiments with diethylcyclopropane, spirooctane, dimethylcyclopropane, and diethylcyclopropane are consistent with a restricted active site for AlkB... - Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-CAllen M Orville
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
Acta Crystallogr D Biol Crystallogr 60:1289-91. 2004..6 A. The asymmetric unit is likely to contain at least two XenB molecules (V(M) = 3.1 A(3) Da(-1), 60% solvent) and a molecular-replacement solution has been determined in order to solve the structure... - Geometric and electronic structure studies of the binuclear nonheme ferrous active site of toluene-4-monooxygenase: parallels with methane monooxygenase and insight into the role of the effector proteins in O2 activationJennifer K Schwartz
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 130:7098-109. 2008....
Research Grants
- ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATIONBrian Fox; Fiscal Year: 2007..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
- ROLE OF IRON OXO CLUSTERS IN FATTY ACID DESATURATIONBrian Fox; Fiscal Year: 2003..The proposed studies will also reveal the molecular details of reaction with non-natural trans fatty acids, which are increasingly found in human diet from the consumption of partially hydrogenated plant oils. ..
- IRON OXO CLUSTERS AND FATTY ACID DESATURATIONBrian Fox; Fiscal Year: 1999..Both of these outcomes will help to further define the principles which govern O2-activation by the proteins containing iron-oxo clusters. ..