Research Topics
Species | David EyreSummaryAffiliation: University of Washington Country: USA Publications
Research Grants
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Detail Information
Publications
Biochemical markers of bone turnover and their association with bone marrow lesionsDavid J Hunter
Department of Epidemiology and Biostatistics, Boston University School of Medicine, Albany Street, Boston, Massachusetts 02118, USA
Arthritis Res Ther 10:R102. 2008..Our objective was to determine whether markers of bone resorption and formation could serve as markers for the presence of bone marrow lesions (BMLs)...- Cartilage markers and their association with cartilage loss on magnetic resonance imaging in knee osteoarthritis: the Boston Osteoarthritis Knee StudyDavid J Hunter
Clinical Epidemiology Research and Training Unit, Boston University School of Medicine, Boston, MA, USA
Arthritis Res Ther 9:R108. 2007..With the exception of COMP, if changes in cartilage turnover in patients with symptomatic knee OA are associated with cartilage loss, they do not appear to affect systemic biomarker levels... - The Helix-II epitope: a cautionary tale from a cartilage biomarker based on an invalid collagen sequenceD R Eyre
University of Washington, Department of Orthopaedics and Sports Medicine, 1959 NE Pacific Street, HSB BB1052, P O Box 356500, Seattle, WA 98195 6500, USA
Osteoarthritis Cartilage 17:423-6. 2009..An apparent database error in the sequence underlying the Helix-II cartilage biomarker immunoassay was investigated at the protein level... - Collagen of articular cartilageDavid Eyre
Orthopaedic Research Laboratories, Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195 6500, USA
Arthritis Res 4:30-5. 2002..Collagen X is normally restricted to the thin layer of calcified cartilage that interfaces articular cartilage with bone... - Collagen polymorphisms of the intervertebral discD R Eyre
University of Washington, Orthopaedic Research Laboratories, Box 356500, Seattle 98195 6500, USA
Biochem Soc Trans 30:844-8. 2002.... - Recent developments in cartilage research: matrix biology of the collagen II/IX/XI heterofibril networkD R Eyre
University of Washington, Orthopaedic Research Laboratories, Box 356500, Seattle 98195 6500, USA
Biochem Soc Trans 30:893-9. 2002..From the structure of the cartilage collagen heteropolymer we also predict a pivotal role for telopeptide (non-triple-helical) proteolytic cleavages in the remodelling and degradation of collagen fibrils... - Covalent cross-linking of the NC1 domain of collagen type IX to collagen type II in cartilageDavid R Eyre
Orthopaedic Research Laboratories, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 279:2568-74. 2004.... - Articular cartilage collagen: an irreplaceable framework?D R Eyre
University of Washington, Dept of Orthopaedics and Sports Medicine, Box 356500, Seattle, WA 98195 6500, USA
Eur Cell Mater 12:57-63. 2006.... - Advances in collagen cross-link analysisDavid R Eyre
Orthopaedic Research Labs, Department of Orthopaedics and Sports Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195 6500, USA
Methods 45:65-74. 2008..g., degrees of lysine hydroxylation and glycosylation) and preferred intermolecular binding partners for telopeptide and helical cross-linking domains in regulating cross-link type and placement... - Maturation of collagen Ketoimine cross-links by an alternative mechanism to pyridinoline formation in cartilageDavid R Eyre
Orthopedic Research Laboratories, Department of Orthopedics and Sports Medicine, University of Washington, Seattle, Washington 98195 6500, USA
J Biol Chem 285:16675-82. 2010..We proposed the trivial name arginoline for the ketoimine cross-link derivative. Mature bovine articular cartilage contains about equimolar amounts of arginoline and hydroxylysyl pyridinoline based on peptide yields... - The kyphoscoliotic type of Ehlers-Danlos syndrome (type VI): differential effects on the hydroxylation of lysine in collagens I and II revealed by analysis of cross-linked telopeptides from urineDavid Eyre
Orthopaedic Research Laboratories, University of Washington, Box 356500, Seattle, WA 98195 6500, USA
Mol Genet Metab 76:211-6. 2002..The presented approach of analyzing urinary cross-linked C-telopeptide fragments of type II collagen may allow the detection of chondrodysplasias due to genetic defects in lysyl hydroxylase isoforms active in cartilage... - Structurally abnormal type II collagen in a severe form of Kniest dysplasia caused by an exon 24 skipping mutationM A Weis
Orthopaedic Research Laboratories, University of Washington, Seattle, Washington 98195 6500, USA
J Biol Chem 273:4761-8. 1998.... - Osteoclasts generate cross-linked collagen N-telopeptides (NTx) but not free pyridinolines when cultured on human boneS Apone
Ostex International, Inc, Seattle, WA 98134, USA
Bone 21:129-36. 1997.... - Bone biomarkers as tools in osteoporosis managementD R Eyre
Department of Orthopaedics, University of Washington, Seattle, USA
Spine (Phila Pa 1976) 22:17S-24S. 1997..The telopeptide markers (NTx and CTx) appear to be the most specific and responsive markers of systemic osteoclast activity... - Sport-related differences in biomarkers of bone resorption and cartilage degradation in endurance athletesE Hutchinson
Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA 98195-6500, USA
Osteoarthritis Cartilage 14:71-6. 2006..The results suggest that crew undergo the highest bone remodeling and runners the highest cartilage degradation. The results also show how these markers can vary physiologically between individuals, at extremes of skeletal exercise... - The elastin-like protein matrix of lamprey branchial cartilage is cross-linked by lysyl pyridinolineR J Fernandes
Department of Orthopaedics, University of Washington, Seattle, Washington, 98195, USA
Biochem Biophys Res Commun 261:635-40. 1999..Pyridinoline cross-links are considered to be characteristic of collagen, so their presence in an elastin-like protein in a primitive cartilage poses evolutionary questions about the tissue, the protein, and the cross-linking mechanism... - Incorporation of structurally defective type II collagen into cartilage matrix in kniest chondrodysplasiaR J Fernandes
Department of Orthopaedics, University of Washington, Seattle, Washington, 98195, USA
Arch Biochem Biophys 355:282-90. 1998..Short deletions (single or partial exon) clustered in one region of the alpha1(II) chain are favored, resulting in abnormal heterotrimeric molecules that become a significant component of the cartilage extracellular matrix... - Incomplete processing of type II procollagen by a rat chondrosarcoma cell lineR J Fernandes
Department of Orthopaedics, University of Washington, Seattle 98195, USA
Eur J Biochem 247:620-4. 1997..The findings indicate that the RCS-LTC cell line fails to express an active type II procollagen N-proteinase and, therefore, offers a useful culture system in which to study the role of N-propeptide removal in fibrillogenesis... - Proteolysis of human bone collagen by cathepsin K: characterization of the cleavage sites generating by cross-linked N-telopeptide neoepitopeL M Atley
Orthopaedic Research Laboratories, University of Washington, Seattle 98195 6500, USA
Bone 26:241-7. 2000..Because cathepsin K appears to be essential for the normal resorption of mineralized bone matrix by osteoclasts, these findings help explain the specificity and responsiveness of NTx as a marker of osteoclastic bone resorption in vivo... - Matrilin-3 forms disulfide-linked oligomers with matrilin-1 in bovine epiphyseal cartilageJ J Wu
Orthopaedic Research Laboratories, University of Washington, Seattle, Washington 98195, USA
J Biol Chem 273:17433-8. 1998..The results indicate a molecular stoichiometry of (matrilin-1)2(matrilin-3)2... - Structural analysis of cross-linking domains in cartilage type XI collagen. Insights on polymeric assemblyJ J Wu
Department of Orthopaedics, University of Washington, Seattle 98195, USA
J Biol Chem 270:18865-70. 1995..This structural feature provides a potential mechanism for the proteolytic depolymerization of type XI collagen by proteases that can cleave between the cross-link and the triple helix (e.g. stromelysin)... - Identification of cross-linking sites in bovine cartilage type IX collagen reveals an antiparallel type II-type IX molecular relationship and type IX to type IX bondingJ J Wu
Orthopaedic Research Laboratories, University of Washington, Seattle 98195
J Biol Chem 267:23007-14. 1992..These data provide a molecular basis for the proposed function of type IX collagen as a critical contributor to the mechanical stability and resistance to swelling of the collagen type II fibril framework of cartilage... - Collagen type IX from human cartilage: a structural profile of intermolecular cross-linking sitesM Diab
Department of Orthopaedics, University of Washington, Seattle 98195 6500, USA
Biochem J 314:327-32. 1996..We also present evidence that, if the proteoglycan form of type IX collagen is present in human cartilage, it can only be a minor component of the matrix, similar to findings with bovine cartilage... - Molecular properties of matrilin-3 isolated from human growth cartilageD Kleemann-Fischer
Department of Orthopaedics, University of Washington, Seattle 98195, USA
Arch Biochem Biophys 387:209-15. 2001..Immunohistochemistry of tissue sections confirmed that both matrilin-1 and matrilin-3 are widely codistributed throughout human skeletal growth cartilage... - Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxisR J Fernandes
Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195 6500, USA
J Biol Chem 276:31502-9. 2001..The findings provide an explanation for the sparing of cartilage in dermatosparaxis and, perhaps, for the relative sparing of some procollagen I-containing tissues... - Evidence that serum NTx (collagen-type I N-telopeptides) can act as an immunochemical marker of bone resorptionJ D Clemens
Ostex International, Inc, Seattle, WA 98134, USA
Clin Chem 43:2058-63. 1997..g., by degradative processes occurring later in the liver and kidney... - Fibroblast growth factor-18 is a trophic factor for mature chondrocytes and their progenitorsJ L Ellsworth
ZymoGenetics, Inc, 1201 Eastlake Avenue East, Seattle, Washington 98102, USA
Osteoarthritis Cartilage 10:308-20. 2002..In addition, the expressions of Fgf18 and Fgf receptors (Fgfr) in adult human articular cartilage were examined... - The association of lumbar spondylolisthesis with collagen IX tryptophan allelesY Matsui
Orthopaedic Research Laboratories, Department of Orthopaedics and Sports Medicine, University of Washington, P.O. Box 356500, Seattle, Washington 98195-6500, USA
J Bone Joint Surg Br 86:1021-6. 2004..81, 95% CI 1.47 to 41.95). Our findings indicate that tryptophan polymorphisms predispose carriers to the development of symptomatic spinal stenosis associated with spondylolisthesis which requires surgery... - Short-term calcium supplementation has no effect on biochemical markers of bone remodeling in early postmenopausal womenU Ulrich
Department of Obstetrics and Gynecology, University of Washington School of Medicine, Seattle, Washington, USA
Arch Gynecol Obstet 270:230-4. 2004..This study was designed to assess changes in biochemical markers of bone remodeling in early postmenopausal women receiving calcium supplementation... - Bone remodeling and bone mineral density during pregnancyU Ulrich
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA
Arch Gynecol Obstet 268:309-16. 2003.... - The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cellsRussell J Fernandes
Department of Orthopaedics and Sports Medicine, Box 356500, University of Washington, Seattle, WA 98195 6500, USA
Bone 40:1343-51. 2007.... - Sp1 family of transcription factors regulates the human alpha2 (XI) collagen gene (COL11A2) in Saos-2 osteoblastic cellsTomohiro Goto
Department of Orthopaedics, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
J Bone Miner Res 21:661-73. 2006..The findings indicate both ubiquitous and osteoblast-specific mechanisms of collagen gene regulation... - Spondylocheiro dysplastic form of the Ehlers-Danlos syndrome--an autosomal-recessive entity caused by mutations in the zinc transporter gene SLC39A13Cecilia Giunta
Division of Metabolism and Molecular Pediatrics, University Children s Hospital, CH 8032 Zurich, Switzerland
Am J Hum Genet 82:1290-305. 2008.... - Analysis of cartilage biomarkers in the early phases of canine experimental osteoarthritisJohn R Matyas
University of Calgary, Calgary, Alberta, Canada
Arthritis Rheum 50:543-52. 2004..To study 3 body fluids for changes in the levels of 5 biomarkers of cartilage metabolism during the early phases of experimental osteoarthritis (OA)... - Assembly of collagen types II, IX and XI into nascent hetero-fibrils by a rat chondrocyte cell lineRussell J Fernandes
Department of Orthopedics and Sports Medicine, University of Washington, Seattle, WA 98195, USA
Eur J Biochem 270:3243-50. 2003.... - Protein consequences of the Col2a1 C-propeptide mutation in the chondrodysplastic Dmm mouseRussell J Fernandes
Orthopaedic Research Laboratories, Department of Orthopaedics and Sports Medicine, Box 356500, University of Washington, Seattle, WA 98195, USA
Matrix Biol 22:449-53. 2003..The integrity of the C-propeptide is thus crucial for the biosynthesis of normal type II collagen by chondrocytes... - The heterozygous disproportionate micromelia (dmm) mouse: morphological changes in fetal cartilage precede postnatal dwarfism and compared with lethal homozygotes can explain the mild phenotypeRobert E Seegmiller
Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602, USA
J Histochem Cytochem 56:1003-11. 2008..Thus, intracellular entrapment of proalpha1(II) seems to be primarily responsible for the dominant-negative effect of the Dmm mutation in the expression of dwarfism... - Matrix deposition of tryptophan-containing allelic variants of type IX collagen in developing human cartilageYoshito Matsui
Orthopaedic Research Laboratories, Department of Orthopaedics and Sports Medicine, University of Washington, P O Box 356500, Seattle, WA 98195 6500, USA
Matrix Biol 22:123-9. 2003..Any pathological consequences are likely, therefore, to be long-term and indirect rather than from overt misassembly of matrix... - Intervertebral disc collagen. Usage of the short form of the alpha1(IX) chain in bovine nucleus pulposusJiann Jiu Wu
Orthopedic Research Laboratories, University of Washington, Seattle 98195, USA
J Biol Chem 278:24521-5. 2003..As in cartilage, type IX collagen of nucleus pulposus was heavily cross-linked to type II collagen and to other molecules of type IX collagen with a similar site occupancy... - Collagen XI chain misassembly in cartilage of the chondrodysplasia (cho) mouseRussell J Fernandes
Departments of Orthopaedics and Sports Medicine, Orthopaedic Research Laboratory, University of Washington, Seattle, WA 98195, USA
Matrix Biol 26:597-603. 2007.... - Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfectaWayne A Cabral
Bone and Extracellular Matrix Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Genet 39:359-65. 2007..Proband collagen secretion was moderately delayed, but total collagen secretion was increased. Prolyl 3-hydroxylase 1 is therefore crucial for bone development and collagen helix formation... - CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfectaRoy Morello
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
Cell 127:291-304. 2006..In humans, CRTAP mutations are associated with the clinical spectrum of recessive osteogenesis imperfecta, including the type II and VII forms. Hence, dysregulation of prolyl 3-hydroxylation is a mechanism for connective tissue disease... - From biomarker to surrogate outcome to osteoarthritis--what are the challenges?L Stephan Lohmander
Lund University Hospital, Lund, Sweden
J Rheumatol 32:1142-3. 2005 - The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritisL Stefan Lohmander
Lund University Hospital, Lund, Sweden
Arthritis Rheum 48:3130-9. 2003..To determine concentrations of crosslinked C-telopeptide fragments of type II collagen (CTX-II) in synovial fluid (SF) from patients with joint injury, osteoarthritis (OA), or other knee arthritides... - COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERGYoshito Matsui
Department of Orthopedics and Sports Medicine, University of Washington School of Medicine, Seattle, Washington 98195 6500, USA
J Biol Chem 278:11369-75. 2003..Taken together, these findings indicate that the COL11A2 gene is regulated both by potential ERG association with a histone deacetylase complex and by direct EWS/ERG recruitment of RNA polymerase II... - Differential expression of aggrecan mRNA isoforms by chondrosarcoma cellsYoshito Matsui
Department of Orthopaedic Surgery, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
Anticancer Res 22:4169-72. 2002..Each corresponds to a subdomain, epidermal growth factor 1(EGF1), EGF2, and a short complement repeat (SCR)... - Deficiency of cartilage-associated protein in recessive lethal osteogenesis imperfectaAileen M Barnes
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
N Engl J Med 355:2757-64. 2006....
Research Grants
- PATHOLOGY OF INBORN SKELETAL DISEASESDavid Eyre; Fiscal Year: 1999....
- PATHOLOGY OF INBORN SKELETAL DISEASESDavid Eyre; Fiscal Year: 2004..By defining molecular mechanisms whereby genetic factors cause joints to fail leads on novel therapeutic and preventive measures are anticipated. ..
- COLLAGENS OF CARTILAGE AND THE INTERVERTEBRAL DISCDavid Eyre; Fiscal Year: 2007..Since mechanical failure of the collagen framework of both tissues is a key, irreversible feature of age-related joint degeneration, new insights on molecular mechanisms may offer the basis of therapeutic advances. ..
- COLLAGEN CROSS-LINKING IN SKELETAL AGING AND DISEASEDavid Eyre; Fiscal Year: 2009..abstract_text> ..
- Collagen Diversity and Pathobiology in Skeletal TissuesDavid R Eyre; Fiscal Year: 2010..With this knowledge new targets for therapy and prevention of genetic and acquired human disorders of bones and joints are predicted. ..
- PATHOLOGY OF INBORN SKELETAL DISEASESDavid Eyre; Fiscal Year: 1993..Through an understanding of the effects of rare inborn mutations, so the normal properties of collagens and their variations can be better appreciated...
- BIOCHEMISTRY OF THE INTERVERTEBRAL DISCDavid Eyre; Fiscal Year: 1993..The basic studies in this proposal are aimed at an improved knowledge at the molecular level of how this complex collagen framework is assembled, how cells maintain it in the mature tissue and what happens to it in degeneration...
- COLLAGEN CROSS-LINKING IN SKELETAL AGING AND DISEASEDavid R Eyre; Fiscal Year: 2010..In parallel with basic tissue studies, collagen fragments in urine are being examined by similar methods as potential non-invasive biomarkers of a patient's bone and joint health. ..