Research Topics
Species | KEVIN ELICEIRISummaryAffiliation: University of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separaseJOSHUA N BEMBENEK
University of Wisconsin Madison, Laboratory of Molecular Biology, 1525 Linden Drive, Madison, WI 53706, USA
Development 134:3837-48. 2007..These observations lead us to speculate that SEP-1 has two separable yet coordinated functions: to regulate cortical granule exocytosis and to mediate chromosome separation...- Collagen density promotes mammary tumor initiation and progressionPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
BMC Med 6:11. 2008..Importantly, regions of high breast density are associated with increased stromal collagen. Thus, the influence of the extracellular matrix on breast carcinoma development and the underlying molecular mechanisms are not understood... - Collagen reorganization at the tumor-stromal interface facilitates local invasionPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI, USA
BMC Med 4:38. 2006.... - Tools for visualizing multidimensional images from living specimensKevin W Eliceiri
Laboratory for Optical and Computational Instrumentation, University of Wisconsin Madison, Madison, WI, USA
Photochem Photobiol 81:1116-22. 2005.... - Analysis of multidimensional biological image dataK W Eliceiri
University of Wisconsin-Madison, Madison, WI, USA
Biotechniques 33:1268-73. 2002 - Molecular expressions: exploring the world of optics and microscopy. http://microscopy.fsu.eduKevin W Eliceiri
Laboratory for Optical and Computational Instrumentation University of Wisconsin Madison 1675 Observatory Drive Madison, WI 53706, USA
Biol Cell 96:403-5. 2004.... - WormClassroom.org: an inquiry-rich educational web portal for research resources of Caenorhabditis elegansFong Mei Lu
Department of Curriculum and Instruction, School of Education, University of Wisconsin Madison, Madison, WI 53706, USA
CBE Life Sci Educ 6:98-108. 2007..elegans researchers; results and comments were used for site improvement. We are actively encouraging continued resource contributions from the C. elegans research and education community for the further development of WormClassroom... - Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganizationPaolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
Biophys J 95:5374-84. 2008.... - Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironmentPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Clin Exp Metastasis 26:357-70. 2009..Herein, we review recent developments of multiphoton FLIM and SLIM to study 2D and 3D cell migration, invasion into the tumor microenvironment, and metastasis... - CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryoKraig T Kumfer
Laboratorie of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 21:266-77. 2010..CHIN-1 restricted NMY-2 recruitment to the anterior during maintenance phase, consistent with its role in polarizing CDC-42 activity during this phase... - Student learning of early embryonic development via the utilization of research resources from the nematode Caenorhabditis elegansFong Mei Lu
Center for Biology Education, University of Wisconsin Madison, Madison, WI 53706 1574, USA
CBE Life Sci Educ 7:64-73. 2008..Furthermore, this study indicates that combining authentic research resources with inquiry-based learning benefits student learning of key concepts in embryology... - Visualization approaches for multidimensional biological image dataCurtis T Rueden
Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, WI 53706, USA
Biotechniques 43:31, 33-6. 2007..As the field has gotten more quantitative in its approaches, this need has only increased with the necessity of having a way to represent key attributes of the data in an open manner... - Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancerCurtis T Rueden
Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009..In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data... - Simultaneous two-photon spectral and lifetime fluorescence microscopyDamian K Bird
Laboratory for Optical and Computational Instrumentation, Department of Molecular Biology, 271 Animal Sciences, 1675 Observatory Drive, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Appl Opt 43:5173-82. 2004..The instrument provides a unique visualization of cellular structures and processes through spectrally and temporally resolved information and may ultimately find applications in live cell and tissue imaging... - Filamin A-beta1 integrin complex tunes epithelial cell response to matrix tensionScott Gehler
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Mol Biol Cell 20:3224-38. 2009..Our findings support a role for FLNa-beta1 integrin as a mechanosensitive complex that bidirectionally senses the tension of the matrix and, in turn, regulates cellular contractility and response to this matrix tension... - Shining new light on 3D cell motility and the metastatic processPaolo P Provenzano
Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Trends Cell Biol 19:638-48. 2009.... - Wavelet compression of three-dimensional time-lapse biological image dataH Narfi Stefansson
Department of Mathematics and Computer Science, University of Wisconsin, Madison, WI 53706, USA
Microsc Microanal 11:9-17. 2005..This will allow animation to be used as a powerful aid to visualizing dynamic changes in three-dimensional structures... - Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancerPaolo P Provenzano
University of Wisconsin, Departments of Pharmacology and Biomedical Engineering and Laboratory for Optical and Computational Instrumentation and Paul P Carbone Comprehensive Cancer Center, Madison, Wisconsin 53706, USA
J Biomed Opt 13:031220. 2008..Using SLIM, we present a method for imaging and differentiating signals from multiple fluorophores that can have overlapping spectra via SLIM Plotter-a computational tool for visualizing and analyzing large spectral-lifetime data sets... - Metadata matters: access to image data in the real worldMelissa Linkert
Laboratory for Optical and Computational Instrumentation, Department of Molecular Biology, Graduate School, University of Wisconsin at Madison, Madison, WI 53711, USA
J Cell Biol 189:777-82. 2010..We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository... - Applications of combined spectral lifetime microscopy for biologyLong Yan
Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, Madison, WI, USA
Biotechniques 41:249, 251, 253 passim. 2006.... - Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancerPaolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
Am J Pathol 173:1551-65. 2008..Therefore, FAK acts as a primary node in the activated signaling network in transformed motile cells and is a prime candidate for novel therapeutic interventions to treat aggressive human breast cancers... - Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breastMatthew W Conklin
Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
Cell Biochem Biophys 53:145-57. 2009..Most forms of cancer have altered metabolism and redox ratios; here we present a method that has potential for early detection of these changes, which are preserved in fixed tissue samples such as classic histopathology slides... - BioClips of symmetric and asymmetric cell divisionFong Mei Lu
Laboratory of Molecular Biology, University of Wisconsin Madison, Madison, WI 53706, USA
Biol Cell 99:289-95. 2007..wormclassroom.org/cb/bioclip)... - Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devicesKyung Eun Sung
Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
Biomaterials 30:4833-41. 2009..In addition, the presented method lays the foundation for high throughput 3D cellular screening... - Bimolecular fluorescence complementation analysis of eukaryotic fusion productsHo Pi Lin
Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
Biol Cell 102:525-37. 2010..The interaction of said proteins after cell fusion produces a fluorescent signal, enabling the identification and tracking of fusion products over time... - Analysis of histology specimens using lifetime multiphoton microscopyKevin W Eliceiri
University of Wisconsin-Madison, Laboratory for Optical and Computational Instrumentation and Laboratory of Molecular Biology, Madison, Wisconsin 53706, USA
J Biomed Opt 8:376-80. 2003..Our findings demonstrate that lifetime multiphoton microscopy has considerable potential as a diagnostic tool for pathological investigations... - VisBio: a computational tool for visualization of multidimensional biological image dataCurtis Rueden
Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, Madison, WI, USA
Traffic 5:411-7. 2004..To address these concerns, we have developed VisBio, an application and toolkit for visualization and analysis of multidimensional, biological image data of any dimensionality... - Nonlinear optical imaging of cellular processes in breast cancerPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Microsc Microanal 14:532-48. 2008..Furthermore, the ability to record and monitor the intrinsic fluorescence and SHG signals provides a unique tool for researchers to understand key events in cancer progression in its natural context... - Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissuesMelissa C Skala
Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin, USA
Cancer Res 65:1180-6. 2005..This study provides the groundwork for further exploration into the application of multiphoton fluorescence endoscopy in a clinical setting... - Visualization of morphological and molecular features associated with chronic ischemia in bioengineered human skinErin M Gill
Department of Biomedical Engineering, University of Wisconsin, Madison, 53706, USA
Microsc Microanal 16:117-31. 2010..We propose that these tissue changes are representative of the ischemic condition and that our experimental model system is appropriate for addressing mechanisms of susceptibility to chronic wounds... - Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADHDamian K Bird
Laboratory for Optical and Computational Instrumentation, Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Cancer Res 65:8766-73. 2005..Our studies suggest that the fluorescence lifetime of both the free and the protein-bound components of NADH and the ratio of free to protein-bound NADH is related to changes in the NADH/NAD+ ratio... - Lrrc10 is required for early heart development and function in zebrafishKi Hyun Kim
Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, 1300 University Avenue, Madison, WI 53706, USA
Dev Biol 308:494-506. 2007..Taken together, the present study demonstrates that Lrrc10 is necessary for normal cardiac development and cardiac function in zebrafish embryos, which will enhance our understanding of congenital heart defects and heart disease...
Research Grants
- Upgrade of Femtosecond Laser Sources for Optical WorkstationKEVIN ELICEIRI; Fiscal Year: 2007..The requested laser sources will benefit several health related imaging projects ranging from studying cell division to understanding the role of signaling in breast cancer. ..