Research Topics
| Gary M DunnySummaryAffiliation: University of Minnesota Country: USA Publications
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Publications
The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolutionGary M Dunny
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
Philos Trans R Soc Lond B Biol Sci 362:1185-93. 2007..In addition, the system may serve as a useful bacterial model for addressing the evolution of biological complexity...- Multicellular behavior in bacteria: communication, cooperation, competition and cheatingGary M Dunny
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455 0312, USA
Bioessays 30:296-8. 2008..Here we discuss the significance of two recent studies of the phenomenon of "cheating" mutants and their exploitation of cooperating microbial populations of Pseudomonas aeruginosa... - Regulatory circuits controlling enterococcal conjugation: lessons for functional genomicsGary M Dunny
Department of Microbiology, University of Minnesota, 420 Delaware St SE, Minneapolis, MN 55455, USA
Curr Opin Microbiol 14:174-80. 2011..The pheromone system may serve as a useful paradigm to guide comprehensive functional genomic analysis of E. faecalis... - Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptidesBriana K Kozlowicz
Department of Microbiology, 1460 Mayo Memorial Building, University of Minnesota, Minneapolis, MN 55455, USA
Mol Microbiol 62:958-69. 2006..We now show that both of these peptides interact with the same binding pocket of PrgX, but they differentially alter the conformation of the protein and its oligomerization state, resulting in opposing biological activities... - Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulenceOlivia N Chuang
Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
Infect Immun 77:539-48. 2009.... - Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01Yuqing Chen
Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Mol Microbiol 63:1549-64. 2007..Specificity of conjugative DNA processing in these plasmids involves both DNA-protein and protein-protein interactions... - Specific control of endogenous cCF10 pheromone by a conserved domain of the pCF10-encoded regulatory protein PrgY in Enterococcus faecalisJosephine R Chandler
Department of Microbiology, University of Minnesota, Minneapolis, 55455, USA
J Bacteriol 187:4830-43. 2005..The combined genetic and physiological data suggest that PrgY may sequester or inactivate cCF10 as it is released from the membrane... - Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgYJosephine R Chandler
Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
J Bacteriol 190:1172-83. 2008..Despite evidence that both PrgY and Eep associate with cCF10 in or near the membrane, results presented here indicate that these two proteins function independently... - Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10Jack H Staddon
Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455-0312, USA
Plasmid 56:102-11. 2006..Therefore, this transfer system may have applications for gene delivery to a variety of poorly-transformed bacteria... - Enterococcus faecalis pheromone-responsive protein PrgX: genetic separation of positive autoregulatory functions from those involved in negative regulation of conjugative plasmid transferBriana K Kozlowicz
Department of Microbiology, 1460 Mayo Memorial Bldg, University of Minnesota, MN 55455, USA
Mol Microbiol 54:520-32. 2004..We propose a new model for the mechanism used by PrgX for regulation of the prgQ promoter, PrgX autoregulation, and Qa RNA processing... - Pheromone-inducible conjugation in Enterococcus faecalis: a model for the evolution of biological complexity?Briana K Kozlowicz
Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, MMC196, 420 Delaware St, SE, Minneapolis, MN 55455, USA
Int J Med Microbiol 296:141-7. 2006.... - Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesisKristi L Frank
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
Infect Immun 80:539-49. 2012..This work illustrates the use of a RIVET screen to provide information about the temporal activation of genes during infection, resulting in the identification and confirmation of a new virulence determinant in an important pathogen... - Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone inductionHelmut Hirt
Department of Microbiology, Medical School, University of Minnesota, Minneapolis, USA
J Bacteriol 187:1044-54. 2005..This observation could have relevance for the virulence of E. faecalis... - Conserved target for group II intron insertion in relaxase genes of conjugative elements of gram-positive bacteriaJack H Staddon
Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA
J Bacteriol 186:2393-401. 2004..Additional support for this mechanism comes from sequence analysis of the insertion sites of the E.c.I4 family of bacterial group II introns... - Characterization of cis-acting prgQ mutants: evidence for two distinct repression mechanisms by Qa RNA and PrgX protein in pheromone-inducible enterococcal plasmid pCF10Taeok Bae
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
Mol Microbiol 51:271-81. 2004..Thus, even though the synthesis of these negative regulators is coupled, they each act independently on separate targets to regulate expression of conjugation functions... - Analysis of the amino acid sequence specificity determinants of the enterococcal cCF10 sex pheromone in interactions with the pheromone-sensing machineryKathryn R Fixen
Department of Microbiology, University of Minnesota, MMC 196, Minneapolis, MN 55455, USA
J Bacteriol 189:1399-406. 2007..The results provide functional data to complement ongoing structural studies of PrgX and increase our understanding of the functional interactions of cCF10 and iCF10 with the pheromone-sensing machinery of pCF10... - Functional genomics of Enterococcus faecalis: multiple novel genetic determinants for biofilm formation in the core genomeKatie S Ballering
Department of Microbiology, University of Minnesota, 1460 Mayo Bldg, MMC196, 420 Delaware St, SE, Minneapolis, MN 55455 0312, USA
J Bacteriol 191:2806-14. 2009.... - Development of a host-genotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalisChristopher J Kristich
Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Plasmid 57:131-44. 2007..Thus, the advanced tools for genetic manipulation of E. faecalis reported here enable efficient and sophisticated genetic analysis of these important pathogens... - Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalisKe Shi
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 102:18596-601. 2005..Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression... - Direct evidence for control of the pheromone-inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript-driven attenuation mechanismChristopher M Johnson
Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA
J Bacteriol 192:1634-42. 2010..Shokeen, C. M. Johnson, T. J. Greenfield, D. A. Manias, G. M. Dunny, and K. E. Weaver, submitted for publication)... - Convergent transcription confers a bistable switch in Enterococcus faecalis conjugationAnushree Chatterjee
Department of Chemical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 108:9721-6. 2011..Moreover, given that convergent transcription is conserved across species, the mechanism of coupling RNAP collision and antisense interaction is likely to have a significant regulatory role in gene expression... - Relation between antibiotic susceptibility and ultrastructure of Staphylococcus aureus biofilms on surgical sutureCarol L Wells
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455, USA
Surg Infect (Larchmt) 12:297-305. 2011..Experiments were designed to clarify the association between antibiotic resistance and biofilm ultrastructure... - Development and use of an efficient system for random mariner transposon mutagenesis to identify novel genetic determinants of biofilm formation in the core Enterococcus faecalis genomeChristopher J Kristich
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
Appl Environ Microbiol 74:3377-86. 2008..These results provide significant new information about the genetics of enterococcal biofilm formation and demonstrate the general utility of our transposon system for functional genomic analysis of E. faecalis... - A paracrine peptide sex pheromone also acts as an autocrine signal to induce plasmid transfer and virulence factor expression in vivoJosephine R Chandler
Department of Microbiology, University of Minnesota, MMC 196, 420 Delaware Street SE, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 102:15617-22. 2005..Although cCF10 has traditionally been viewed as an intercellular signal, these results show that pCF10 has also adapted cCF10 as an autocrine signal that activates expression of virulence and conjugation functions... - Two targets in pCF10 DNA for PrgX binding: their role in production of Qa and prgX mRNA and in regulation of pheromone-inducible conjugationTaeok Bae
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
J Mol Biol 315:995-1007. 2002..On the basis of these results, we propose that both DNA binding sites are required for the autoregulation of PrgX expression and for positive regulation of Qa RNA... - Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteinsChristopher M Waters
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
J Bacteriol 185:3613-23. 2003..These new functions attributed to GelE suggest that it acts to increase the dissemination of E. faecalis in high-density environments... - The aggregation domain of aggregation substance, not the RGD motifs, is critical for efficient internalization by HT-29 enterocytesChristopher M Waters
Department of Microbiology, University of Minnesota Medical School, 1420 Delaware Street SE, Minneapolis, MN 55455, USA
Infect Immun 71:5682-9. 2003..These data show that Asc10 directs internalization of E. faecalis into HT-29 enterocytes through a non-RGD-dependent mechanism... - Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutantChristopher J Kristich
420 Delaware St. S.E, MMC196, Minneapolis, MN 55455, USA
Appl Environ Microbiol 71:5837-49. 2005..faecalis cells required for high-frequency conjugative plasmid transfer in liquid matings. The system of markerless exchange reported here will facilitate detailed genetic analysis of these important pathogens... - Quantitative analysis of group II intron expression and splicing in Lactococcus lactisYuqing Chen
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA
Appl Environ Microbiol 71:2576-86. 2005..Interestingly, LtrB protein showed a tendency to function in cis on its oriT target. The low level of ltrB transcript and relatively inefficient splicing of the intron may limit Ll.ltrB mobility and dissemination in nature... - Characterization of a Staphylococcus aureus surface virulence factor that promotes resistance to oxidative killing and infectious endocarditisNatalia Malachowa
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA
Infect Immun 79:342-52. 2011..These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections... - A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactisJoanna R Klein
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
J Bacteriol 186:1991-8. 2004..Once the system was validated for the engineered mutants, random mutagenesis of the intron followed by genetic and molecular screening for splicing defects resulted in identification of point mutations that affect splicing... - Acceleration of Enterococcus faecalis biofilm formation by aggregation substance expression in an ex vivo model of cardiac valve colonizationOlivia N Chuang-Smith
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
PLoS ONE 5:e15798. 2010..Interference with the molecular interactions involved in adherence and initiation of biofilm development in vivo with specific inhibitory compounds could lead to more effective treatment of infectious endocarditis... - Bacterial contamination of surgical suture resembles a biofilmMichelle J Henry-Stanley
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA
Surg Infect (Larchmt) 11:433-9. 2010..The purpose of this study was to compare the kinetic development of Staphylococcus aureus and Enterococcus faecalis on five surgical suture materials and to clarify factors that might influence this growth... - Formation of vegetations during infective endocarditis excludes binding of bacterial-specific host antibodies to Enterococcus faecalisJohn K McCormick
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
J Infect Dis 185:994-7. 2002.... - A eukaryotic-type Ser/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistenceChristopher J Kristich
Department of Microbiology and Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 104:3508-13. 2007.... - Enterococcal peptide sex pheromones: synthesis and control of biological activityJosephine R Chandler
Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg, 420 Delaware Street SE, Minneapolis, MN 55455-0312, USA
Peptides 25:1377-88. 2004.... - Esp-independent biofilm formation by Enterococcus faecalisChristopher J Kristich
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
J Bacteriol 186:154-63. 2004..faecalis. In summary, E. faecalis forms complex biofilms by a process that is sensitive to environmental conditions and does not require the Esp surface protein... - ccfA, the genetic determinant for the cCF10 peptide pheromone in Enterococcus faecalis OG1RFMichelle H Antiporta
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455-0312, USA
J Bacteriol 184:1155-62. 2002..faecalis and also resulted in cCF10 production by Lactococcus lactis, a non-pheromone producer. Site-directed mutagenesis of the ccfA sequence encoding the cCF10 peptide confirmed that ccfA was a functional genetic determinant for cCF10... - High-resolution visualization of the microbial glycocalyx with low-voltage scanning electron microscopy: dependence on cationic dyesStanley L Erlandsen
Deptartment of Genetics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
J Histochem Cytochem 52:1427-35. 2004.... - In vivo induction of virulence and antibiotic resistance transfer in Enterococcus faecalis mediated by the sex pheromone-sensing system of pCF10Helmut Hirt
Department of Microbiology, University of Minnesota, Medical School, Minneapolis, Minnesota 55455, USA
Infect Immun 70:716-23. 2002..The data suggested that the mechanism of in vivo induction may involve interference of plasma with the normal function of the pheromone peptide and its inhibitor... - Bacterial group II introns and their association with mobile genetic elementsJoanna R Klein
Department of Microbiology, University of Minnesota Medical School, 1460 Mayo Bldg/Box 196, 420 Delaware St. SE, Minneapolis, MN 55455-0312, USA
Front Biosci 7:d1843-56. 2002..Limited functional data is available for most of these introns, but sequence analysis points out several common themes, most notably that bacterial group II introns are almost always carried on mobile genetic elements... - Group effort in toxin synthesisGary M Dunny
Nature 415:33-4. 2002 - Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediateYuqing Chen
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
J Bacteriol 190:3632-45. 2008.... - Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine proteaseAgathe Bourgogne
Division of Infectious Disease, Center for the Study of Emerging and Re-Emerging Pathogens, MSB 2.112, University of Texas Medical School, 6431 Fannin St, Houston, TX 77030, USA
J Bacteriol 188:2875-84. 2006....