Research Topics
| DIRK DITTMERSummaryAffiliation: University of North Carolina Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Efficiency clustering for low-density microarrays and its application to QPCREric F Lock
Department of Statistics and Operations Research, University of North Carolina, Chapel Hill, NC, USA
BMC Bioinformatics 11:386. 2010..To achieve reliable quantification all primer pairs or array probes must perform with the same efficiency...- Rapalogs in viral cancersDirk P Dittmer
The University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, CB 7290, 715 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7290, USA 919 966 7960 919 962 8103
Expert Opin Investig Drugs 21:135-8. 2012..We also speculate how the molecular makeup of viral cancers could guide the selection and use of known and novel mTOR inhibitors to treat virus-associated malignancies... - Restricted Kaposi's sarcoma (KS) herpesvirus transcription in KS lesions from patients on successful antiretroviral therapyDirk P Dittmer
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, and Center for AIDS Research, University of North Carolina, Chapel Hill, North Carolina, USA
MBio 2:e00138-11. 2011..This implies a novel mode of pathogenesis (tightly latent KS), which may inform KS cancer treatment options in the post-ART era... - Multiple pathways for Epstein-Barr virus episome loss from nasopharyngeal carcinomaDirk P Dittmer
Lineberger Comprehensive Cancer Center, Center for AIDS Research and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA
Int J Cancer 123:2105-12. 2008..The conceptual insights provided by our quantitative analysis should aid our understanding of mammalian episomes, as well as lead to designs to cure latent viral infection... - Kaposi's sarcoma in the era of HAART-an update on mechanisms, diagnostics and treatmentDirk P Dittmer
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599 7290, USA
AIDS Rev 7:56-61. 2005..Today we know a lot about KSHV and can begin to understand, diagnose and treat KS as a viral disease rather than another sarcoma... - Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcomaAndrea J O'Hara
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
PLoS Pathog 5:e1000389. 2009..Mir-140 and Kaposi sarcoma-associated herpesvirus viral miRNAs increased linearly with the degree of transformation. Mir-24 emerged as a biomarker specific for KS... - Transcription profile of Kaposi's sarcoma-associated herpesvirus in primary Kaposi's sarcoma lesions as determined by real-time PCR arraysDirk P Dittmer
Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA
Cancer Res 63:2010-5. 2003..Stratification by KSHV lytic transcription can identify lesions with a high percentage of lytically infected cells, which may respond to antiviral drugs... - Faster quantitative real-time PCR protocols may lose sensitivity and show increased variabilityChelsey Hilscher
Department of Microbiology and Immunology and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, NC, USA
Nucleic Acids Res 33:e182. 2005..Fast PCR was associated with a loss of sensitivity as well as higher variability, but not with a loss of specificity or with a higher false positive rate... - Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirusWuguo Chen
University of North Carolina at Chapel Hill, CB 7290, 715 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7290, USA
J Virol 85:9495-505. 2011..The fact that RPS6 has a well-established nuclear function beyond its role in ribosome assembly suggests that RPS6 (and by extension other ribosomal proteins) contributes to the extraordinary stability of LANA... - KSHV-associated disease in the AIDS patientDirk P Dittmer
Department of Microbiology and Immunology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA
Cancer Treat Res 133:129-39. 2007 - Regulation and autoregulation of the promoter for the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirusJoseph H Jeong
University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, Oklahoma 73104, USA
J Biol Chem 279:16822-31. 2004..Furthermore, we demonstrate that LANA regulates its own promoter via its C-terminal domain and does bind to a defined site within the core promoter... - Toll-like receptor signaling controls reactivation of KSHV from latencySean M Gregory
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 106:11725-30. 2009..Our findings have broad implications for physiological triggers of latent viral infections, such as herpesviral reactivation and persistence in the host... - Gene alteration and precursor and mature microRNA transcription changes contribute to the miRNA signature of primary effusion lymphomaAndrea J O'Hara
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, and Curriculum in Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Blood 111:2347-53. 2008..This defines the miRNA signature of PEL and shows that transcriptional regulation of pre-miRNA as well as mature miRNA levels contribute nonredundant information that can be used for the classification of human tumors... - Liposomal daunorubicin as treatment for Kaposi's sarcomaChristin E Petre
Lineberger Comprehensive Cancer Center, Center for AIDS Research and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599 7290, USA
Int J Nanomedicine 2:277-88. 2007.... - Targeted therapy for Kaposi's sarcoma and Kaposi's sarcoma-associated herpesvirusDirk P Dittmer
Department of Microbiology and Immunology, Center for AIDS Research and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Curr Opin Oncol 19:452-7. 2007..To summarize major recent findings on the biology of human herpesvirus-8, i.e. Kaposi's sarcoma-associated herpesvirus, and the implications of these findings for Kaposi's sarcoma treatment... - Real-time quantitative PCR analysis of viral transcriptionJames Papin
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Methods Mol Biol 292:449-80. 2005..Because these arrays are RT-PCR-based, they are inherently more sensitive and robust than current hybridization-based approaches and are ideally suited to query viral gene expression in models of pathogenesis... - Maribavir inhibits epstein-barr virus transcription in addition to viral DNA replicationFu Zhang Wang
Departments of Microbiology and Immunology and Medicine, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
J Virol 83:12108-17. 2009..The results emphasize the different modes of action of the two drugs and suggest that the action of MBV may be linked to indirect effects on the transcription of EBV genes through the interaction of BGLF4 with multiple viral proteins... - Rapamycin is efficacious against primary effusion lymphoma (PEL) cell lines in vivo by inhibiting autocrine signalingSang Hoon Sin
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, NC 27599 7290, USA
Blood 109:2165-73. 2007..This validates sirolimus as a new treatment option for PEL... - Distinct p53, p53:LANA, and LANA complexes in Kaposi's Sarcoma--associated Herpesvirus LymphomasWuguo Chen
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill, CB no 7290, 715 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7290, USA
J Virol 84:3898-908. 2010..If p53 is activated by DNA damage or nutlin-3a, the complex falls apart easily, and p53 exercises its role as guardian of the genome... - Viral latent proteins as targets for Kaposi's sarcoma and Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) induced lymphomaMichelle R Staudt
Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd, Oklahoma City, OK 73104, USA
Curr Drug Targets Infect Disord 3:129-35. 2003..In particular Rta/orf50 is necessary and sufficient for lytic replication and deserves serious consideration as a target for KSHV-specific antivirals... - Whole-genome transcription profiling of rhesus monkey rhadinovirusDirk P Dittmer
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, North Carolina 27599, USA
J Virol 79:8637-50. 2005..Using this assay, we demonstrate that the genome-wide transcription profile for RRV closely parallels the genome-wide transcription profile for KSHV... - Tumor suppressor microRNAs are underrepresented in primary effusion lymphoma and Kaposi sarcomaAndrea J O'Hara
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research at University of North Carolina at Chapel Hill, NC 27599 7290, USA
Blood 113:5938-41. 2009..Because many tumor suppressor proteins are wild-type in KS and PEL, down-regulation of multiple tumor suppressor miRNAs provides a novel, alternative mechanism of transformation... - Novel Kaposi's sarcoma-associated herpesvirus homolog in baboonsDenise Whitby
Viral Epidemiology Section, AIDS Vaccine Program, SAIC-Frederick, National Cancer Institute, Frederick, MD 21702, USA
J Virol 77:8159-65. 2003..This establishes baboons as a novel resource to investigate rhadinovirus biology, which can be developed into an animal model system for KSHV-associated human diseases, vaccine development, and therapy evaluation... - De novo generation of CD4 T cells against viruses present in the host during immune reconstitutionTomas Kalina
Fred Hutchinson Cancer Research Center and the University of Washington School of Medicine, Seattle, WA, USA
Blood 105:2410-4. 2005..This finding provides a strong rationale to improve thymopoiesis in recipients of hematopoietic cell transplants and, perhaps, in other persons lacking de novo-generated CD4 T cells, such as AIDS patients and elderly persons... - EBV microRNAs in primary lymphomas and targeting of CXCL-11 by ebv-mir-BHRF1-3Tianli Xia
The Viral Oncology Program, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA
Cancer Res 68:1436-42. 2008..Targeted suppression of CXCL-11/I-TAC by a viral-encoded miRNA may serve as an immunomodulatory mechanism in these tumors... - More on HIV-associated Kaposi's sarcomaSusan E Krown
N Engl J Med 358:535-6; author reply 536. 2008 - In vivo-restricted and reversible malignancy induced by human herpesvirus-8 KSHV: a cell and animal model of virally induced Kaposi's sarcomaAgata D Agostino Mutlu
Laboratory of Viral Oncogenesis, Division of Hematology Oncology, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
Cancer Cell 11:245-58. 2007..These results show that KSHV malignancy is in vivo growth restricted and reversible, defining mECK36 as a biologically sensitive animal model of KSHV-dependent KS... - Reactivation of Kaposi's sarcoma-associated herpesvirus by natural products from Kaposi's sarcoma endemic regionsDenise Whitby
Viral Epidemiology Section, AIDS Vaccine Program, SAIC Frederick, NCI Frederick, Frederick, MD 21702, USA
Int J Cancer 120:321-8. 2007..These in vitro data provide support for the "oncoweed" hypothesis by demonstrating basic biological plausibility... - Long-term-infected telomerase-immortalized endothelial cells: a model for Kaposi's sarcoma-associated herpesvirus latency in vitro and in vivoFeng-Qi An
Department of Molecular Genetics and Microbiology, University of Florida, Shands Cancer Center, Gainesville, FL 32610-0232, USA
J Virol 80:4833-46. 2006..KSHV-infected LTCs represent the first xenograft model for KS and should be of use to study KS pathogenesis and for the validation of anti-KS drug candidates... - Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteinsGerman P Horn
, Klinikum der Philipps, , Marburg, Germany
J Virol 79:1911-7. 2005..Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei... - Role of Notch signal transduction in Kaposi's sarcoma-associated herpesvirus gene expressionHeesoon Chang
Department of Microbiology and Molecular Genetics and Tumor Virology Division, New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
J Virol 79:14371-82. 2005.... - Azidothymidine inhibits NF-kappaB and induces Epstein-Barr virus gene expression in Burkitt lymphomaMotoki Kurokawa
Department of Dermatology, Medical College, University of Miyazaki, Japan
Blood 106:235-40. 2005..AZT inhibits NF-kappaB and up-regulates EBV gene expression in primary EBV+ BLs. AZT with hydroxyurea may represent an inexpensive, targeted regimen for endemic BL...
Research Grants
- ART Modulation of Viral Pathogenesis in Oral EpitheliaDirk P Dittmer; Fiscal Year: 2010..e. TLR signaling by using our recently developed NF-kappaB real-time QPCR array to compare both clinical samples and experimental models of buccal and gingival epithelium. ..
- ART Modulation of Viral Pathogenesis in Oral EpitheliaDIRK DITTMER; Fiscal Year: 2007..e. TLR signaling by using our recently developed NF-kappaB real-time QPCR array to compare both clinical samples and experimental models of buccal and gingival epithelium. ..
- Regulation of the KSHV latent promoterDIRK DITTMER; Fiscal Year: 2007....
- Primate Model for Viral Pathogenesis in the Oral CavityDIRK DITTMER; Fiscal Year: 2006..abstract_text> ..
- Unified high throughput diagnosticsDIRK DITTMER; Fiscal Year: 2003..It utilizes the established capability in bacterial genomics at the University of Oklahoma Health Sciences Center and is greatly motivated by Oklahoma City's unfortunate experience with a large-scale terrorist attack. ..
- In situ real-time PCR arrays for viral-associated canc*DIRK DITTMER; Fiscal Year: 2005..In KS lesions tumor progression depends on and responds to viral gene expression. This study will provide a road map for a better understanding of KS biology and clinical classification of KS tumors. ..
- Real-time high throughput PCR for microbial pathogensDIRK DITTMER; Fiscal Year: 2003..Heuristic computations would increase or decrease the degeneracy of primers to reduce false positive and increase the possibility to amplify more distantly related sequences. ..
- Human gamma herpes virus DNA vaccinesDIRK DITTMER; Fiscal Year: 2002..This experience and the immunologic evaluations performed will hopefully be preparatory for a successful trial of one or more of these gamma herpes virus vaccines in man. ..
- Regulation of the KSHV latent promoterDirk P Dittmer; Fiscal Year: 2010..Understanding their regulation will help unravel the biology of cancers caused by KSHV and, we hope, provide novel targets for intervention. ..