Research Topics
Species | J J CullenSummaryAffiliation: University of Iowa Country: USA Publications
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Publications
The role of manganese superoxide dismutase in the growth of pancreatic adenocarcinomaJoseph J Cullen
Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Cancer Res 63:1297-303. 2003..Overexpression of MnSOD may be effective in growth suppression of pancreatic cancer...- Ascorbate induces autophagy in pancreatic cancerJoseph J Cullen
Department of Surgery, University of Iowa College of Medicine, Iowa City, IA, USA
Autophagy 6:421-2. 2010..Thus, it is clear that intravenous administration of ascorbate can yield very high plasma levels, while oral treatment does not... - Dicumarol inhibition of NADPH:quinone oxidoreductase induces growth inhibition of pancreatic cancer via a superoxide-mediated mechanismJoseph J Cullen
Department of Surgery, University of Iowa College of Medicine, Iowa City, IA, USA
Cancer Res 63:5513-20. 2003..These mechanisms suggest that altering the intracellular redox environment of pancreatic cancer cells may inhibit growth and delineate a potential strategy directed against pancreatic cancer... - Lipopolysaccharide temporarily impairs sphincter of Oddi motilityJ J Cullen
Department of Surgery, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
Nitric Oxide 5:547-54. 2001..Inducible nitric oxide synthase mRNA was readily seen in SO segments after LPS. LPS impairs EFS-induced relaxation and increases baseline tone of the SO. The effects of LPS on SO motility appear to be mediated by nitric oxide... - Expression of antioxidant enzymes in diseases of the human pancreas: another link between chronic pancreatitis and pancreatic cancerJoseph J Cullen
Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa, USA
Pancreas 26:23-7. 2003..There are three major types of primary intracellular antioxidant enzymes in mammalian cells: superoxide dismutase (SOD), catalase, and peroxidase, of which glutathione peroxidase is the most prominent... - Suppression of the malignant phenotype in human pancreatic cancer cells by the overexpression of manganese superoxide dismutaseChristine Weydert
Department of Radiation Oncology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Mol Cancer Ther 2:361-9. 2003..These results suggest that MnSOD may be a tumor suppressor gene in human pancreatic cancer. Delivery of the MnSOD gene may prove beneficial for suppression of pancreatic cancer growth... - Role of Rac1-dependent NADPH oxidase in the growth of pancreatic cancerJ Du
Department of Radiation Oncology, University of Iowa College of Medicine, Iowa City, IA, USA
Cancer Gene Ther 18:135-43. 2011..These results suggest that activation of Rac1-dependent superoxide generation leads to pancreatic cancer cell proliferation. In pancreatic cancer, inhibition of Rac1 may be a potential therapeutic target... - Suppression of the malignant phenotype in pancreatic cancer by overexpression of phospholipid hydroperoxide glutathione peroxidaseJingru Liu
Department of Radiation Oncology, University of Iowa College of Medicine, and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA
Hum Gene Ther 17:105-16. 2006..AdPhGPx-Lform decreased in vivo tumor growth to a greater extent than did AdPhGPx-S-form. Because of the growthinhibitory effects of PhGPx, lipid hydroperoxides may play an important role in the growth of pancreatic cancer... - Redox regulation of pancreatic cancer cell growth: role of glutathione peroxidase in the suppression of the malignant phenotypeJingru Liu
Department of Radiation Oncology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Hum Gene Ther 15:239-50. 2004..GPx may be a tumor suppressor gene in pancreatic cancer. Delivery of the GPx gene alone or in combination with the MnSOD gene may prove beneficial for treatment of pancreatic cancer... - Effect of endotoxin on opossum oesophageal motor functionH Park
Department of Internal Medicine, University of Iowa, College of Medicine and VA Medical Center, Iowa City, USA
Neurogastroenterol Motil 12:215-21. 2000..The increase in plasma nitrite/nitrate was attenuated by AG. These studies support the hypothesis that endotoxin modulates oesophageal motor function by increasing NO production and suggest that this results from the induction of iNOS... - Metastatic progression of pancreatic cancer: changes in antioxidant enzymes and cell growthAnne Lewis
University of Iowa College of Medicine, Iowa City, 52242, USA
Clin Exp Metastasis 22:523-32. 2005..Metastatic disease is associated with changes in the content and activity of antioxidant enzymes with an associated change in growth characteristics depending on the O(2) concentrations... - Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cellsJuan Du
Department of Surgery and University of Iowa College of Medicine, Holden Comprehensive Cancer Center, and Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA
J Biol Chem 281:37416-26. 2006..We conclude mitochondrial production of reactive oxygen species mediates the increased susceptibility of cancer cells to dicumarol-induced cytotoxicity... - A prospective study of outcomes, healthcare resource utilization, and costs associated with postoperative nosocomial infectionsLoreen A Herwaldt
Department of Internal Medicine, University of Iowa College of Public Health, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Infect Control Hosp Epidemiol 27:1291-8. 2006..We evaluated 4 important outcomes associated with postoperative nosocomial infection: costs, mortality, excess length of stay, and utilization of healthcare resources... - Treatment of pancreatic cancer cells with dicumarol induces cytotoxicity and oxidative stressAnne Lewis
Department of Surgery, Radiation Oncology and Holden Comprehensive Cancer Center, and University of Iowa College of Medicine, Iowa City, Iowa, USA
Clin Cancer Res 10:4550-8. 2004..CONCLUSIONS: Inhibition of NQO(1) with dicumarol induces cell killing and oxidative stress in pancreatic cancer cells and speculate that dicumarol may prove to be useful in pancreatic cancer therapeutics... - Inhibition of cell growth by overexpression of manganese superoxide dismutase (MnSOD) in human pancreatic carcinomaMatthew Ough
University of Iowa College of Medicine, Iowa City, IA, USA
Free Radic Res 38:1223-33. 2004..These results suggest that MnSOD may be a tumor suppressor gene in human pancreatic cancer... - Endotoxin temporarily impairs canine colonic absorption of water and sodiumJ J Cullen
Surgical Service, VAMC, Iowa City, Iowa, USA
J Surg Res 74:34-8. 1998..We conclude that endotoxin temporarily impairs postprandial colonic absorption, which may be due to the rapid liquid transit that occurs. These effects may contribute to the diarrhea seen during and after septic episodes... - Mechanisms of ascorbate-induced cytotoxicity in pancreatic cancerJuan Du
Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Clin Cancer Res 16:509-20. 2010..Pharmacologic concentrations of ascorbate may be effective in cancer therapeutics. We hypothesized that ascorbate concentrations achievable with i.v. dosing would be cytotoxic in pancreatic cancer for which the 5-year survival is <3%... - Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissueChristine J Weydert
Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine, Iowa City, Iowa, USA
Nat Protoc 5:51-66. 2010..Other techniques including immunohistochemistry and immunogold can further evaluate the levels of the various antioxidant enzymes in tissues and cells. In general, these assays require 24-48 h to complete... - Human pancreatic carcinoma cells activate maspin expression through loss of epigenetic controlMatthew Fitzgerald
Department of Radiation Oncology, Free Radical and Radiation Biology Program, University of Iowa, Iowa City, IA 52242, USA
Neoplasia 5:427-36. 2003..Thus, human pancreatic carcinoma cells acquire maspin expression through epigenetic derepression of the maspin locus, and in so doing appear to co-opt a normal cellular mechanism for the regulation of this gene... - Targeting NAD(P)H:quinone oxidoreductase (NQO1) in pancreatic cancerAnne M Lewis
University of Iowa College of Medicine, Iowa City, Iowa, USA
Mol Carcinog 43:215-24. 2005..The effects of streptonigrin were reversed in pancreatic cancer cells pretreated with dicumarol, a known inhibitor of NQO1. NQO1 may be a therapeutic target in pancreatic cancer where survival is measured in months... - Modulation of reactive oxygen species in pancreatic cancerMelissa L T Teoh
Department of Surgery and Radiation Oncology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Clin Cancer Res 13:7441-50. 2007..e., manganese superoxide dismutase (MnSOD), copper zinc superoxide dismutase (CuZnSOD), and extracellular superoxide dismutase (EcSOD)] on the malignant phenotype of human pancreatic cancer... - Are gastric, jejunal, or both forms of enteral feeding gastroprotective during stress?K S Ephgrave
Veterans Administration Medical Center, Iowa City, Iowa 52246, USA
J Surg Res 88:1-7. 2000..The purpose of these studies was to determine if jejunal nutrients can protect without gastric contact and to determine if gastric pH, motility, or mucosal perfusion is affected... - Preoperative risk factors for nasal carriage of Staphylococcus aureusLoreen A Herwaldt
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242-1081, USA
Infect Control Hosp Epidemiol 25:481-4. 2004..aureus nasal carriage. It remains to be seen whether preoperative weight loss would reduce the rate of nasal carriage. In addition, the value of screening this patient population for S. aureus nasal carriage merits further investigation... - Enhancing the antitumor activity of adriamycin and ionizing radiationWenqing Sun
Free Radical and Radiation Biology Program, Department of Radiation Oncology, and Holden Comprehensive Cancer Center, Carver College of Medicine, The University of Iowa, and VA Medical Center, Iowa City, IA 52242, USA
Cancer Res 69:4294-300. 2009..The results suggest that agents that produce O(2)(*-) in combination with AdMnSOD plus BCNU may represent a powerful new antitumor regimen against breast cancer... - 2-deoxy-D-glucose causes cytotoxicity, oxidative stress, and radiosensitization in pancreatic cancerMitchell C Coleman
Department of Radiation Oncology, University of Iowa, Iowa City, IA 52242, USA
Free Radic Biol Med 44:322-31. 2008.... - Intranasal mupirocin to prevent postoperative Staphylococcus aureus infectionsTrish M Perl
University of Iowa Colleges of Medicine and Public Health, Iowa City, USA
N Engl J Med 346:1871-7. 2002..aureus surgical-site infections overall, but it did significantly decrease the rate of all nosocomial S. aureus infections among the patients who were S. aureus carriers... - Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossumHyojin Park
Department of Internal Medicine, University of Iowa College of Medicine and VA Medical Center, Iowa City, USA
J Gastroenterol 37:1000-4. 2002..CONCLUSIONS: These studies support the hypothesis that endotoxin increases NO production by the induction of iNOS protein and mRNA... - Preventing postoperative Staphylococcus infections: an updateMatthew Grady
Department of Surgery, University of Iowa College of Medicine, and Veterans Affairs Medical Center, Iowa City, IA, USA
Surg Technol Int 11:57-60. 2003..Mupirocin, a topical antibiotic effective against Gram-positive organisms, was proved to be effective in reducing the rates of nasal colonization of S. aureus and decreased postoperative nosocomial infections due to S. aureus... - Preoperative risk factors for postoperative Staphylococcus aureus nosocomial infectionsSandy Fang
Department of Surgery, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa, USA
Surg Technol Int 13:35-8. 2004..Thus, by modulating these variables, investigators may create interventions aimed at reducing S. aureus nasal carriage and ultimately, postoperative nosocomial infections... - Efficacy of beta-lapachone in pancreatic cancer treatment: exploiting the novel, therapeutic target NQO1Matthew Ough
Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Cancer Biol Ther 4:95-102. 2005.... - Mechanisms of impaired gallbladder contractile response in chronic acalculous cholecystitisAnders R Merg
Department of Surgery, University of Iowa College of Medicine, Iowa City, IA, USA
J Gastrointest Surg 6:432-7. 2002..The impaired gallbladder emptying in chronic acalculous cholecystitis appears to be due to diminished spontaneous contractile activity and decreased contractile responsiveness to both CCK and EFS... - Mitochondrial DNA depletion induces radioresistance by suppressing G2 checkpoint activation in human pancreatic cancer cellsCarla R Cloos
Departments of Surgery, Iowa City, Iowa, USA
Radiat Res 171:581-7. 2009..These results suggest that mitochondrial function may regulate cell cycle checkpoint activation and radiosensitivity in pancreatic cancer cells... - Mitochondrial ROS and radiation induced transformation in mouse embryonic fibroblastsChangbin Du
Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA
Cancer Biol Ther 8:1962-71. 2009..These results support the hypothesis that SOD2 activity and mitochondria generated ROS regulate IR induced transformation in mouse embryonic fibroblasts... - Extracellular redox state regulates features associated with prostate cancer cell invasionLuksana Chaiswing
Pathology and Laboratory Medicine Service, William S Middleton Memorial Veterans Hospital, Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53705, USA
Cancer Res 68:5820-6. 2008..Our results suggest a prominent role of extracellular redox status in regulation of cell invasion, which may provide opportunities for therapeutic interventions...
Research Grants
- Mechanistic Role of Peroxynitrite in Gallbladder DiseaseJOSEPH CULLEN; Fiscal Year: 2004..In addition, it is expected that the results will fundamentally advance the field of free radical biology and gallbladder physiology. ..
- Mechanisms of Dicumarol-Induced CytotoxicityJOSEPH CULLEN; Fiscal Year: 2007..pancreatic cancer cells. ..