Gregory J Crowther

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi A mechanism-based whole-cell screening assay to identify inhibitors of protein export in Escherichia coli by the Sec pathway
    Gregory J Crowther
    University of Washington, Seattle, WA, USA
    J Biomol Screen 17:535-41. 2012
  2. ncbi Using science songs to enhance learning: an interdisciplinary approach
    Gregory Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    CBE Life Sci Educ 11:26-30. 2012
  3. ncbi Poem: A is for alanine
    Gregory J Crowther
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195
    Biochem Mol Biol Educ 33:418. 2005
  4. ncbi Identification of attractive drug targets in neglected-disease pathogens using an in silico approach
    Gregory J Crowther
    Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS Negl Trop Dis 4:e804. 2010
  5. ncbi Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands
    Gregory J Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195 7185, USA
    J Biomol Screen 14:700-7. 2009
  6. ncbi Use of thermal melt curves to assess the quality of enzyme preparations
    Gregory J Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Anal Biochem 399:268-75. 2010
  7. ncbi TDR Targets: a chemogenomics resource for neglected diseases
    María P Magariños
    Instituto de Investigaciones Biotecnologicas, Universidad de San Martin, San Martin, Buenos Aires, Argentina
    Nucleic Acids Res 40:D1118-27. 2012
  8. ncbi Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy
    Ludmila Chistoserdova
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 189:9076-81. 2007
  9. ncbi Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds
    Gregory J Crowther
    University of Washington, Seattle, WA, USA
    Mol Biochem Parasitol 175:21-9. 2011
  10. ncbi Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline
    Stephen N Hewitt
    Seattle Structural Genomics Center for Infectious Disease SSGCID, University of Washington, WA 98195, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1006-9. 2011

Research Grants

  1. Modular design of central metabolism in methylotrophs
    Gregory Crowther; Fiscal Year: 2006

Collaborators

Detail Information

Publications16

  1. ncbi A mechanism-based whole-cell screening assay to identify inhibitors of protein export in Escherichia coli by the Sec pathway
    Gregory J Crowther
    University of Washington, Seattle, WA, USA
    J Biomol Screen 17:535-41. 2012
    ..Testing of current antibiotics confirmed that they do not generally act through the Sec pathway. A mini-screen of 800 compounds indicated the assay's readiness for larger screening projects...
  2. ncbi Using science songs to enhance learning: an interdisciplinary approach
    Gregory Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    CBE Life Sci Educ 11:26-30. 2012
    ..The challenges ahead include 1) defining the circumstances in which music is most likely to promote learning and 2) developing rubrics for evaluating the quality of songs...
  3. ncbi Poem: A is for alanine
    Gregory J Crowther
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195
    Biochem Mol Biol Educ 33:418. 2005
    ..This work is just one of many science songs and poems that may be used for educational purposes. A comprehensive database of over 2,000 songs is available online at www.science-groove.org/MASSIVE/...
  4. ncbi Identification of attractive drug targets in neglected-disease pathogens using an in silico approach
    Gregory J Crowther
    Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS Negl Trop Dis 4:e804. 2010
    ....
  5. ncbi Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands
    Gregory J Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195 7185, USA
    J Biomol Screen 14:700-7. 2009
    ..The authors conclude that buffer optimization to minimize variability in Tm measurements increases the success of thermal melt screens involving proteins for which a standard buffer is suboptimal...
  6. ncbi Use of thermal melt curves to assess the quality of enzyme preparations
    Gregory J Crowther
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Anal Biochem 399:268-75. 2010
    ....
  7. ncbi TDR Targets: a chemogenomics resource for neglected diseases
    María P Magariños
    Instituto de Investigaciones Biotecnologicas, Universidad de San Martin, San Martin, Buenos Aires, Argentina
    Nucleic Acids Res 40:D1118-27. 2012
    ....
  8. ncbi Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy
    Ludmila Chistoserdova
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 189:9076-81. 2007
    ..Mutation of a small open reading frame (fdh4B) downstream of fdh4A resulted in mutant phenotypes similar to the phenotypes of fdh4A mutants, suggesting that fdh4B is also involved in formate oxidation...
  9. ncbi Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds
    Gregory J Crowther
    University of Washington, Seattle, WA, USA
    Mol Biochem Parasitol 175:21-9. 2011
    ..Six of these targets were inhibited by one or more of the antimalarial scaffolds and may have potential use in drug development, further target validation studies and exploration of P. falciparum biochemistry and biology...
  10. ncbi Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline
    Stephen N Hewitt
    Seattle Structural Genomics Center for Infectious Disease SSGCID, University of Washington, WA 98195, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1006-9. 2011
    ..While the behavior of the cleaved proteins was disappointing, further refinements in MBP tagging may permit the more widespread use of MBP-fusion proteins in crystallographic studies...
  11. ncbi Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1
    Gregory J Crowther
    Department of Chemical Engineering, University of Washington, Box 355014, Seattle, Washington 98195 5014, USA
    J Bacteriol 190:5057-62. 2008
    ..These results all support the suggestion that formate, not formaldehyde, is the main branch point for methylotrophic metabolism in M. extorquens AM1...
  12. ncbi A systems biology approach uncovers cellular strategies used by Methylobacterium extorquens AM1 during the switch from multi- to single-carbon growth
    Elizabeth Skovran
    Department of Chemical Engineering, University of Washington, Seattle, Washington, USA
    PLoS ONE 5:e14091. 2010
    ..These different modes of growth utilize dramatically different central metabolic pathways with limited pathway overlap...
  13. ncbi Fiber recruitment affects oxidative recovery measurements of human muscle in vivo
    Gregory J Crowther
    Department of Physiology, University of Washington, Seattle, USA
    Med Sci Sports Exerc 34:1733-7. 2002
    ..We therefore tested the hypothesis that differences in muscle fiber recruitment can cause differences in whole-muscle oxidative recovery from exercise...
  14. ncbi Acidosis inhibits oxidative phosphorylation in contracting human skeletal muscle in vivo
    Sharon A Jubrias
    Department of Radiology, University of Washington Medical Center, Seattle, WA 98195, USA
    J Physiol 553:589-99. 2003
    ..These results show that acidosis inhibits oxidative phosphorylation in vivo and can limit ATP supply in exercising muscle to below the mitochondrial capacity...
  15. ncbi Control of glycolysis in contracting skeletal muscle. II. Turning it off
    Gregory J Crowther
    Department of Physiology and Biophysics, University of Washington Medical Center, Seattle, Washington 98195-7115, USA
    Am J Physiol Endocrinol Metab 282:E74-9. 2002
    ..We conclude that the inactivation of glycolysis after exercise reflects the cessation of contractile activity and is mediated within the glycolytic pathway rather than via the control of glycogen breakdown...
  16. ncbi Control of glycolysis in contracting skeletal muscle. I. Turning it on
    Gregory J Crowther
    Department of Physiology and Biophysics, University of Washington Medical Center, Seattle, Washington 98195-7115, USA
    Am J Physiol Endocrinol Metab 282:E67-73. 2002
    ..We conclude that the delayed onset of glycolytic flux during exercise reflects the time needed to raise metabolites to flux-activating levels...

Research Grants1

  1. Modular design of central metabolism in methylotrophs
    Gregory Crowther; Fiscal Year: 2006
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