Shelley D Copley

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. ncbi A trade-off between catalytic power and substrate inhibition in TCHQ dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 47:3258-65. 2008
  2. ncbi Evolution of efficient pathways for degradation of anthropogenic chemicals
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado, USA
    Nat Chem Biol 5:559-66. 2009
  3. ncbi Toward a systems biology perspective on enzyme evolution
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    J Biol Chem 287:3-10. 2012
  4. ncbi The origin of the RNA world: co-evolution of genes and metabolism
    Shelley D Copley
    University of Colorado at Boulder, CIRES, Campus Box 216, Boulder, CO 80309, USA
    Bioorg Chem 35:430-43. 2007
  5. ncbi Divergence of function in the thioredoxin fold suprafamily: evidence for evolution of peroxiredoxins from a thioredoxin-like ancestor
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 43:13981-95. 2004
  6. ncbi Mechanism of the severe inhibition of tetrachlorohydroquinone dehalogenase by its aromatic substrates
    Joseph R Warner
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:4438-47. 2007
  7. ncbi Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as cosubstrates
    Johannes Rudolph
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Biochemistry 49:9249-55. 2010
  8. ncbi A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activities
    Sean Yu McLoughlin
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute of Environmental Sciences, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 105:13497-502. 2008
  9. ncbi Pre-steady-state kinetic studies of the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Joseph R Warner
    University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:13211-22. 2007
  10. ncbi A dimethyl ketal-protected benzoin-based linker suitable for photolytic release of unprotected peptides
    Nataliya Chumachenko
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    J Org Chem 76:9409-16. 2011

Research Grants

  1. Elucidation and Evolutionary Potential of a Latent Pathway for PLP Synthesis
    Shelley D Copley; Fiscal Year: 2010
  2. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    Shelley D Copley; Fiscal Year: 2010
  3. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    SHELLEY COPLEY; Fiscal Year: 2009
  4. Mechanisms and Evolutionary Potential of Promiscuous Enzyme Activities
    SHELLEY COPLEY; Fiscal Year: 2007
  5. Recruitment of Enzymes to Serve New Functions
    SHELLEY COPLEY; Fiscal Year: 2006
  6. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    Shelley D Copley; Fiscal Year: 2010

Collaborators

Detail Information

Publications22

  1. ncbi A trade-off between catalytic power and substrate inhibition in TCHQ dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 47:3258-65. 2008
    ....
  2. ncbi Evolution of efficient pathways for degradation of anthropogenic chemicals
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado, USA
    Nat Chem Biol 5:559-66. 2009
    ....
  3. ncbi Toward a systems biology perspective on enzyme evolution
    Shelley D Copley
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    J Biol Chem 287:3-10. 2012
    ....
  4. ncbi The origin of the RNA world: co-evolution of genes and metabolism
    Shelley D Copley
    University of Colorado at Boulder, CIRES, Campus Box 216, Boulder, CO 80309, USA
    Bioorg Chem 35:430-43. 2007
    ....
  5. ncbi Divergence of function in the thioredoxin fold suprafamily: evidence for evolution of peroxiredoxins from a thioredoxin-like ancestor
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 43:13981-95. 2004
    ....
  6. ncbi Mechanism of the severe inhibition of tetrachlorohydroquinone dehalogenase by its aromatic substrates
    Joseph R Warner
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:4438-47. 2007
    ..In addition, dissociation of the GSSG product is inhibited by TriCHQ. The thiol-disulfide exchange reaction is the rate-limiting step in the reductive dehalogenation reaction under physiological conditions...
  7. ncbi Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as cosubstrates
    Johannes Rudolph
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Biochemistry 49:9249-55. 2010
    ..7 × 10(6) M?¹s?¹. Additionally, we have characterized the stereochemistry of ?-ketoglutarate reduction by showing that d-2-HGA, but not l-2-HGA, is a competitive inhibitor vs 4PE and a noncompetitive inhibitor vs ?-ketoglutarate...
  8. ncbi A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activities
    Sean Yu McLoughlin
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute of Environmental Sciences, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 105:13497-502. 2008
    ..Notably, overexpression does not require a mutation in the regulatory region of the protein; amino acid limitation attributable to the poor catalytic abilities of E383A ProA causes a physiological response that results in overexpression...
  9. ncbi Pre-steady-state kinetic studies of the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Joseph R Warner
    University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 46:13211-22. 2007
    ....
  10. ncbi A dimethyl ketal-protected benzoin-based linker suitable for photolytic release of unprotected peptides
    Nataliya Chumachenko
    Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    J Org Chem 76:9409-16. 2011
    ..Additionally, we developed a route to a dithiane-protected linker that requires only two steps and proceeds in 65% yield, a significant improvement over previous synthetic routes...
  11. ncbi A mechanism for the association of amino acids with their codons and the origin of the genetic code
    Shelley D Copley
    Cooperative Institute for Research in Environmental Sciences, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 102:4442-7. 2005
    ....
  12. ncbi Three serendipitous pathways in E. coli can bypass a block in pyridoxal-5'-phosphate synthesis
    Juhan Kim
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA
    Mol Syst Biol 6:436. 2010
    ..One step in the pathway may be non-enzymatic...
  13. ncbi Enzymes with extra talents: moonlighting functions and catalytic promiscuity
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder Campus, Box 216, Boulder, CO 80309 0216, USA
    Curr Opin Chem Biol 7:265-72. 2003
    ..New insights into catalytic promiscuity are provided by studies of aminoglycoside kinase (3') type IIIa, tetrachlorohydroquinone dehalogenase, and aldolase antibody 38C2...
  14. ncbi Pentachlorophenol Hydroxylase, a Poorly Functioning Enzyme Required for Degradation of Pentachlorophenol by Sphingobium chlorophenolicum
    Klara Hlouchova
    Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, Colorado 80309, United States
    Biochemistry 51:3848-60. 2012
    ..The conversion of TCHQ to TCBQ reverses flux through the pathway. Substantial uncoupling also occurs during the reaction with TCHQ...
  15. ncbi Why metabolic enzymes are essential or nonessential for growth of Escherichia coli K12 on glucose
    Juhan Kim
    Cooperative Institute for Research in Environmental Sciences and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Biochemistry 46:12501-11. 2007
    ..coli to survive in a variety of habitats and therefore to have a metabolism that allows optimal exploitation of varying environmental resources and synthesis of small molecules when they cannot be obtained from the environment...
  16. ncbi Visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of Escherichia coli
    MingHua Dai
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, CO 80309, USA
    Metab Eng 7:45-52. 2005
    ..This frequency is three-four orders of magnitude better than those previously reported for recombination in fused protoplasts of Gram-negative bacteria such as E. coli and Providencia alcalifaciens...
  17. ncbi Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genes
    Shelley D Copley
    Department of Molecular, Cellular, and Developmental Biology and the Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, CO 80309, USA
    Genome Biol 3:research0025. 2002
    ..To evaluate this, we have used bioinformatics to analyze sequences of the biosynthetic enzymes gamma-glutamylcysteine ligase and glutathione synthetase...
  18. ncbi MotifCluster: an interactive online tool for clustering and visualizing sequences using shared motifs
    Micah Hamady
    Department of Computer Science, University of Colorado, Boulder, CO 80309, USA
    Genome Biol 9:R128. 2008
    ..We demonstrate MotifCluster's accuracy using gold-standard protein superfamilies; using recommended settings, families were assigned to the correct superfamilies with 0.17% false positive and no false negative assignments...
  19. ncbi A mechanistic investigation of the thiol-disulfide exchange step in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Joseph R Warner
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, Colorado 80309-0216, USA
    Biochemistry 44:10360-8. 2005
    ..Remarkably, single-turnover kinetic studies show that the enzyme catalyzes this step by approximately 10000-fold. This high reactivity requires an as yet unidentified protonated group in the active site...
  20. ncbi Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723
    MingHua Dai
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Appl Environ Microbiol 70:2391-7. 2004
    ....
  21. ncbi Characterization of the initial steps in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenase
    Philip M Kiefer
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 41:1315-22. 2002
    ..Possible similarities between this mechanism and the mechanism for isomerization of maleylacetoacetate and maleylpyruvate are discussed...
  22. ncbi The reaction catalyzed by tetrachlorohydroquinone dehalogenase does not involve nucleophilic aromatic substitution
    Philip M Kiefer
    Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biochemistry 41:1308-14. 2002
    ..Third, substantial normal solvent kinetic isotope effects on k(cat) and k(cat)/K(M,TriCHQ) are observed. Nonenzymatic and enzymatic nucleophilic S(N)Ar reactions typically show inverse solvent kinetic isotope effects...

Research Grants12

  1. Elucidation and Evolutionary Potential of a Latent Pathway for PLP Synthesis
    Shelley D Copley; Fiscal Year: 2010
    ..Such pathways could allow degradation of anthropogenic chemicals such as antibiotics, pesticides, and industrial pollutants. ..
  2. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    Shelley D Copley; Fiscal Year: 2010
    ..In addition, this work will inform efforts to engineer enzymes and bacteria to produce chemicals and pharmaceuticals or to degrade pollutants under environmentally benign conditions. ..
  3. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    SHELLEY COPLEY; Fiscal Year: 2009
    ..In addition, this work will inform efforts to engineer enzymes and bacteria to produce chemicals and pharmaceuticals or to degrade pollutants under environmentally benign conditions. ..
  4. Mechanisms and Evolutionary Potential of Promiscuous Enzyme Activities
    SHELLEY COPLEY; Fiscal Year: 2007
    ..Thus, the processes by which new enzyme activities evolve are of great interest for both theoretical and practical reasons. ..
  5. Recruitment of Enzymes to Serve New Functions
    SHELLEY COPLEY; Fiscal Year: 2006
    ....
  6. The Evolutionary Origin and Potential of Newly Recruited Enzymes
    Shelley D Copley; Fiscal Year: 2010
    ..In addition, this work will inform efforts to engineer enzymes and bacteria to produce chemicals and pharmaceuticals or to degrade pollutants under environmentally benign conditions. ..