Research Topics
| S D CopleySummaryAffiliation: University of Colorado Country: USA Publications
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Publications
Enzymes with extra talents: moonlighting functions and catalytic promiscuityShelley D Copley
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder Campus, Box 216, Boulder, CO 80309 0216, USA
Curr Opin Chem Biol 7:265-72. 2003..New insights into catalytic promiscuity are provided by studies of aminoglycoside kinase (3') type IIIa, tetrachlorohydroquinone dehalogenase, and aldolase antibody 38C2...- A trade-off between catalytic power and substrate inhibition in TCHQ dehalogenaseJoseph R Warner
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
Biochemistry 47:3258-65. 2008.... - Identification and localization of a stable sulfenic acid in peroxide-treated tetrachlorohydroquinone dehalogenase using electrospray mass spectrometryW S Willett
Department of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO 80309, USA
Chem Biol 3:851-7. 1996..The damage is reversible by treatment with dithiothreitol. Possible types of oxidative damage include an inappropriate disulfide bond, a cysteine sulfenic acid, or a methionine sulfoxide... - Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genesShelley D Copley
Department of Molecular, Cellular, and Developmental Biology and the Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, CO 80309, USA
Genome Biol 3:research0025. 2002..To evaluate this, we have used bioinformatics to analyze sequences of the biosynthetic enzymes gamma-glutamylcysteine ligase and glutathione synthetase... - Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approachS D Copley
Dept of Chemistry and Biochemistry and Cooperative Institute for Research in Environmental Studies, University of Colorado at Boulder, Boulder, CO 80309, USA
Trends Biochem Sci 25:261-5. 2000..This apparently recently assembled pathway does not function very well: pentachlorophenol hydroxylase is quite slow, and tetrachlorohydroquinone dehalogenase is subject to severe substrate inhibition... - Microbial dehalogenases: enzymes recruited to convert xenobiotic substratesS D Copley
University of Colorado at Boulder, Department of Chemistry and Biochemistry 80309, USA
Curr Opin Chem Biol 2:613-7. 1998.... - Evolution of efficient pathways for degradation of anthropogenic chemicalsShelley D Copley
Department of Molecular, Cellular and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado, USA
Nat Chem Biol 5:559-66. 2009.... - Divergence of function in the thioredoxin fold suprafamily: evidence for evolution of peroxiredoxins from a thioredoxin-like ancestorShelley D Copley
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Biochemistry 43:13981-95. 2004.... - The origin of the RNA world: co-evolution of genes and metabolismShelley D Copley
University of Colorado at Boulder, CIRES, Campus Box 216, Boulder, CO 80309, USA
Bioorg Chem 35:430-43. 2007.... - Mechanism of the severe inhibition of tetrachlorohydroquinone dehalogenase by its aromatic substratesJoseph R Warner
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309, USA
Biochemistry 46:4438-47. 2007..In addition, dissociation of the GSSG product is inhibited by TriCHQ. The thiol-disulfide exchange reaction is the rate-limiting step in the reductive dehalogenation reaction under physiological conditions... - A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activitiesSean Yu McLoughlin
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute of Environmental Sciences, University of Colorado, Boulder, CO 80309, USA
Proc Natl Acad Sci U S A 105:13497-502. 2008..Notably, overexpression does not require a mutation in the regulatory region of the protein; amino acid limitation attributable to the poor catalytic abilities of E383A ProA causes a physiological response that results in overexpression... - MotifCluster: an interactive online tool for clustering and visualizing sequences using shared motifsMicah Hamady
Department of Computer Science, University of Colorado, Boulder, CO 80309, USA
Genome Biol 9:R128. 2008..We demonstrate MotifCluster's accuracy using gold-standard protein superfamilies; using recommended settings, families were assigned to the correct superfamilies with 0.17% false positive and no false negative assignments... - Why metabolic enzymes are essential or nonessential for growth of Escherichia coli K12 on glucoseJuhan Kim
Cooperative Institute for Research in Environmental Sciences and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Biochemistry 46:12501-11. 2007..coli to survive in a variety of habitats and therefore to have a metabolism that allows optimal exploitation of varying environmental resources and synthesis of small molecules when they cannot be obtained from the environment... - A mechanistic investigation of the thiol-disulfide exchange step in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenaseJoseph R Warner
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Campus Box 216, Boulder, Colorado 80309-0216, USA
Biochemistry 44:10360-8. 2005..Remarkably, single-turnover kinetic studies show that the enzyme catalyzes this step by approximately 10000-fold. This high reactivity requires an as yet unidentified protonated group in the active site... - A mechanism for the association of amino acids with their codons and the origin of the genetic codeShelley D Copley
Cooperative Institute for Research in Environmental Sciences, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Proc Natl Acad Sci U S A 102:4442-7. 2005.... - Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723MingHua Dai
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, Colorado 80309, USA
Appl Environ Microbiol 70:2391-7. 2004.... - Characterization of the initial steps in the reductive dehalogenation catalyzed by tetrachlorohydroquinone dehalogenasePhilip M Kiefer
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
Biochemistry 41:1315-22. 2002..Possible similarities between this mechanism and the mechanism for isomerization of maleylacetoacetate and maleylpyruvate are discussed... - The reaction catalyzed by tetrachlorohydroquinone dehalogenase does not involve nucleophilic aromatic substitutionPhilip M Kiefer
Department of Molecular, Cellular, and Developmental Biology and Cooperative Institute for Research in Environmental Sciences, Campus Box 216, University of Colorado at Boulder, Boulder, Colorado 80309, USA
Biochemistry 41:1308-14. 2002..Third, substantial normal solvent kinetic isotope effects on k(cat) and k(cat)/K(M,TriCHQ) are observed. Nonenzymatic and enzymatic nucleophilic S(N)Ar reactions typically show inverse solvent kinetic isotope effects... - Multiple turnovers of the nicotino-enzyme PdxB require ?-keto acids as cosubstratesJohannes Rudolph
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
Biochemistry 49:9249-55. 2010..7 × 10(6) M?¹s?¹. Additionally, we have characterized the stereochemistry of ?-ketoglutarate reduction by showing that d-2-HGA, but not l-2-HGA, is a competitive inhibitor vs 4PE and a noncompetitive inhibitor vs ?-ketoglutarate...