Research Topics
| Stephen CooperSummaryAffiliation: University of Michigan Country: USA Publications
| Collaborators
|
Detail Information
Publications
On a heuristic point of view concerning the expression of numerous genes during the cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA
IUBMB Life 64:10-7. 2012..An alternative view of the cell cycle proposes cycle-independent, invariant accumulation of mRNA during the cell cycle with decreases of specific proteins occurring only during the mitotic period of the cell cycle...- Is whole-culture synchronization biology's 'perpetual-motion machine'?Stephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Trends Biotechnol 22:266-9. 2004.... - Rejoinder: whole-culture synchronization cannot, and does not, synchronize cellsStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor MI 48109 0620, USA
Trends Biotechnol 22:274-6. 2004.... - Control and maintenance of mammalian cell sizeStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
BMC Cell Biol 5:35. 2004..If there is no cell-size-control system, then exponential growth is not allowed, as exponential growth, according to Conlon and Raff, would require a cell-size-control system... - Reanalysis of the protocol for in vitro synchronization of mammalian astrocytic cultures by serum deprivationStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Brain Res Brain Res Protoc 15:115-8. 2005..Thus, the proposed protocol will not produce a synchronized culture... - Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: on the analysis of gene expression during the normal, eukaryotic, cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Theor Biol Med Model 2:47. 2005..are discussed in order to see whether their analysis is related to gene expression during the division cycle... - Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronizationStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Cell Tissue Res 324:237-42. 2006..The current view of cell-cycle control that is based on methods that are not suitable for cell-cycle analysis must therefore be reconsidered when results are based on whole-culture synchronization... - Distinguishing between linear and exponential cell growth during the division cycle: single-cell studies, cell-culture studies, and the object of cell-cycle researchStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Theor Biol Med Model 3:10. 2006..In contrast to Mitchison's viewpoint, it is argued here that the biological laws underlying cell growth are not to be found in single-cell studies. The cellular growth law can and should be understood by studying cells as an aggregate... - Checkpoints and restriction points in bacteria and eukaryotic cellsStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Bioessays 28:1035-9. 2006..Some of the ubiquitous checkpoint phenomena widely described may be merely the result of the inherent incompleteness of earlier events preventing the initiation of subsequent events... - Regulation of DNA synthesis in bacteria: Analysis of the Bates/Kleckner licensing/initiation-mass model for cell cycle controlStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Mol Microbiol 62:303-7. 2006.... - Membrane-elution analysis of content of cyclins A, B1, and E during the unperturbed mammalian cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Cell Div 2:28. 2007..The membrane-elution method is a method that allows the study of the cell cycle by producing a culture of unperturbed, synchronized cells... - Microarrays and the relationship of mRNA variation to protein variation during the cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
J Theor Biol 249:574-81. 2007..We conclude that mRNA variations during the division cycle, as measured by microarrays, cannot by themselves, identify cycle-specific functions related to protein variations... - Thymidine block does not synchronize L1210 mouse leukaemic cells: implications for cell cycle control, cell cycle analysis and whole-culture synchronizationS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Cell Prolif 41:156-67. 2008..Generalizing the results to other cell lines, we suggest that these conclusions call into question experimental measurements of gene expression during the division cycle based on thymidine inhibition synchronization... - On the use of metaphor to understand, explain, or rationalize redundant genes in yeastStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
FEMS Yeast Res 8:345-8; discussion 349-50. 2008..The redundant gene proposal is analyzed, and it is noted that there are many problems with the redundant gene model. An alternative metaphor is suggested to explain the genetic composition of a yeast culture... - On the fiftieth anniversary of the Schaechter, Maaløe, Kjeldgaard experiments: implications for cell-cycle and cell-growth controlStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 5620, USA
Bioessays 30:1019-24. 2008..In particular, the emphasis on steady-state growth conditions, and clear and sharp changes in growth conditions were fundamental to their experiments and have been codified in the principles of the Copenhagen School of Microbiology... - Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cellsStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Cell Biol Int 33:71-7. 2009.... - Invariant mRNA and mitotic protein breakdown solves the Russian Doll problem of the cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0520, USA
Cell Biol Int 33:10-8. 2009..These results, and theoretical ideas support an alternative view of the cell cycle where many of the proposed control systems do not exist... - Rethinking synchronization of mammalian cells for cell cycle analysisS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Cell Mol Life Sci 60:1099-106. 2003..Release of arrested cells from inhibition does not produce cells reflecting cells during the normal division cycle. Thus, cells produced by batch or forcing methods are not experimental models for analysis of the normal cell cycle... - How the change from FLM to FACS affected our understanding of the G1-phase of the cell cycleStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Cell Cycle 2:157-9. 2003..It is suggested that these G1-phase controls and phenomena require a critical reevaluation in the light of an alternative cell-cycle model that does not require or postulate such G1-phase controls... - Division pattern of a round mutant of Escherichia coliS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
J Bacteriol 179:5582-4. 1997..These results differ from a previous proposal that division planes in this round mutant are perpendicular to the prior division plane (W. D. Donachie, S. Addinall, and K. Begg, Bioessays 17:569-576, 1995)... - On the interpretation of the shortening of the G1-phase by overexpression of cyclins in mammalian cellsS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 49109 0620, USA
Exp Cell Res 238:110-5. 1998..Thus, the experiments on cyclin overexpression and their effect on G1-phase length cannot be used to support the proposal that cyclins act specifically during the G1-phase of the division cycle... - On the proposal of a G0 phase and the restriction pointS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 49109 0620, USA
FASEB J 12:367-73. 1998..Their experiments are consistent with the continuum model of the mammalian division cycle. There is no need to postulate a restriction point or a G0 phase to explain the serum starvation results... - Mammalian cells are not synchronized in G1-phase by starvation or inhibition: considerations of the fundamental concept of G1-phase synchronizationS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
Cell Prolif 31:9-16. 1998..However, these cells are not arrested at a particular point in the G1-phase. Analysis of 'G1 arrested cells' suggests that, upon resumption of growth, the cells are not synchronized... - Cycle-specific replication of chromosomal and F-plasmid originsS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
FEMS Microbiol Lett 163:217-22. 1998.... - G1 and S phase gene expression cannot be analyzed in mammalian cells synchronized by inhibitionS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, USA
Microb Comp Genomics 2:269-73. 1997..This proposal has a wide applicability and should not be confined to the specific article under discussion... - Phosphorylation-dephosphorylation of retinoblastoma protein not necessary for passage through the mammalian cell division cycleS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
IUBMB Life 48:225-30. 1999..We propose that phosphorylation-dephosphorylation of Rb is independent of the division cycle and is primarily determined by growth conditions throughout the division cycle... - Revisiting retinoblastoma protein phosphorylation during the mammalian cell cycleS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
Cell Mol Life Sci 58:580-95. 2001..We propose that additional experimental evidence is needed to decide whether there is a G1-phase-specific phosphorylation of Rb protein... - Helical growth and the curved shape of Vibrio choleraeS Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
FEMS Microbiol Lett 198:123-4. 2001..The comma-like shape of V. cholerae is not due to an asymmetrical positioning of peptidoglycan such that some chains of peptidoglycan are placed so they are more spread out on one side of the cell and squeezed together on the other side... - The Schaechter-Bentzon-Maaløe experiment and the analysis of cell cycle events in eukaryotic cellsStephen Cooper
Dept of Microbiology, University of Michigan School of Medicine, Ann Arbor, MI 48109 0620, USA
Trends Microbiol 10:169-73. 2002..The idea of an control experiment under unperturbed conditions is proposed for the analysis of data on cell-cycle-specific gene expression in yeast and mammalian cells... - Minimally disturbed, multicycle, and reproducible synchrony using a eukaryotic "baby machine"Stephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Bioessays 24:499-501. 2002..But most important, the baby machine method now opens the way for the study of the cell cycle of minimally disturbed, artifact-free, well-synchronized, mammalian cells... - Reappraisal of G1-phase arrest and synchronization by lovastatinStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
Cell Biol Int 26:715-27. 2002..Analysis of previous reports of 'synchronization' and growth-arrest support these findings. It is concluded that lovastatin neither synchronizes cells, nor arrests cells in the G1-phase of the division cycle... - Reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest pointsStephen Cooper
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
FASEB J 17:333-40. 2003..It is proposed that the arrest of cells with a particular DNA content equivalent to that in cells in the G1 phase of the division cycle does not mean there is any particular G1 phase control point... - Analysis of cell-cycle gene expression in Saccharomyces cerevisiae using microarrays and multiple synchronization methodsKerby Shedden
Department of Statistics, University of Michigan, Ann Arbor, MI 48109 1285, USA
Nucleic Acids Res 30:2920-9. 2002..Under this alternative explanation, the observed cyclicities in the other three experiments are a stress response to synchronization, and may not reproduce in unperturbed cells... - Analysis of cell-cycle-specific gene expression in human cells as determined by microarrays and double-thymidine block synchronizationKerby Shedden
Department of Statistics, University of Michigan, Ann Arbor, MI 48109-1285, USA
Proc Natl Acad Sci U S A 99:4379-84. 2002..This finding places doubt on the assumption that the cells are actually synchronized. We propose that the microarray results do not support the proposal that there are numerous cell-cycle-specifically expressed genes in human cells... - SDF-1alpha/CXCL12 enhances retroviral-mediated gene transfer into immature subsets of human and murine hematopoietic progenitor cellsW Tao
Department of Microbiology and Immunology, The Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN 46202-5181, USA
Gene Ther 11:61-9. 2004..These results may be of clinical relevance... - Effect of mecillinam on peptidoglycan synthesis during the division cycle of Salmonella typhimurium 2616J Licht
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620
Res Microbiol 144:423-33. 1993..Other antibiotics added at equivalent activities did not show an increase in cross-linking... - Cell cycle analysis and microarraysStephen Cooper
Trends Genet 18:289-90. 2002