Research Topics
| Margaret ClarkeSummaryAffiliation: University of Oklahoma Health Sciences Center Country: USA Publications
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Detail Information
Publications
Distribution of alkaline phosphatase in vegetative dictyostelium cells in relation to the contractile vacuole complexMargaret Clarke
Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, 825 N E 13th Street, Oklahoma City, OK 73104, USA
Eur J Cell Biol 83:289-96. 2004..We discuss these results in relation to the two models of contractile vacuole structure and suggest that all data are consistent with the first model...- Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cellsMargaret Clarke
Oklahoma Medical Research Foundation, Oklahoma City, USA
J Cell Sci 115:2893-905. 2002..VatM-GFP was retrieved from the phagosome membrane prior to exocytosis of the indigestible remnants of the yeast particle. These data suggest that vacuolar proton pumps are recycled by fusion of advanced with newly formed endosomes... - Endosome fusion and microtubule-based dynamics in the early endocytic pathway of dictyosteliumMargaret Clarke
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
Traffic 3:791-800. 2002..Further maturation of endosomes led to loss of pleiomorphy in favor of a spherical shape, inability to fuse with new macropinosomes, and diminished motility... - Mechanically induced actin-mediated rocketing of phagosomesMargaret Clarke
Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
Mol Biol Cell 17:4866-75. 2006..The forces generated by rocketing phagosomes are sufficient to push the entire microtubule apparatus forward and to dislocate the nucleus... - Phagocyte meets prey: uptake, internalization, and killing of bacteria by Dictyostelium amoebaeMargaret Clarke
Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, 825 N E 13th Street, Oklahoma City, OK 73104, USA
Eur J Cell Biol 85:1001-10. 2006..Thus, tracking individual phagosomes has revealed that early steps in phagosome maturation occur much more rapidly than had been inferred from previous population studies... - Retrieval of the vacuolar H-ATPase from phagosomes revealed by live cell imagingMargaret Clarke
Program in Genetic Models of Disease, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America
PLoS ONE 5:e8585. 2010..Similarly, the enzyme is thought to be retrieved from phagosome membranes prior to exocytosis of indigestible material, although that process has not been directly visualized... - Altered expression of the 100 kDa subunit of the Dictyostelium vacuolar proton pump impairs enzyme assembly, endocytic function and cytosolic pH regulationTongyao Liu
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, 825 N E 13th Street, Oklahoma City, OK 73104, USA
J Cell Sci 115:1907-18. 2002..In addition, mutant cells manifested alterations in the dynamic regulation of cytosolic pH that are characteristic of normal cells grown in acid media, which suggested that the V-ATPase also plays a role in cytosolic pH regulation... - Curvature recognition and force generation in phagocytosisMargaret Clarke
Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
BMC Biol 8:154. 2010..We survey mechanisms that enable a phagocyte to remodel actin organization in response to particles of complex shape... - Heterogeneous modes of uptake for latex beads revealed through live cell imaging of phagocytes expressing a probe for phosphatidylinositol-(3,4,5)-trisphosphate and phosphatidylinositol-(3,4)-bisphosphateJennifer Giorgione
Oklahoma Medical Research Foundation, Program in Genetic Models of Disease, Oklahoma City, Oklahoma
Cell Motil Cytoskeleton 65:721-33. 2008..We conclude that caution is needed in extrapolating results from latex bead phagosomes to phagosomes containing physiological substances, especially in early stages of the endocytic pathway... - Dynamic properties of Legionella-containing phagosomes in Dictyostelium amoebaeHao Lu
Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
Cell Microbiol 7:995-1007. 2005..These studies have clarified certain aspects of the infection process and provided new insights into the dynamic interactions between the pathogen and its host... - Mitochondrial membrane dynamics are altered in cluA- mutants of DictyosteliumStephen D Fields
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
J Muscle Res Cell Motil 23:829-38. 2002..Our data suggest that cluA- cells may be blocked at a very late step in fission of the outer mitochondrial membrane... - Recent insights into host-pathogen interactions from DictyosteliumMargaret Clarke
Program in Genetic Models of Disease, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
Cell Microbiol 12:283-91. 2010..The social amoeba Dictyostelium discoideum is a powerful system for analysis of conserved host-pathogen interactions. This report reviews recent insights gained for several bacterial pathogens using Dictyostelium as host... - F-pili dynamics by live-cell imagingMargaret Clarke
Genetic Models of Disease Research Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
Proc Natl Acad Sci U S A 105:17978-81. 2008..Additionally and unexpectedly, we infer that extension and retraction are accompanied by rotation about the long axis of the filament... - Tubular-vesicular transformation in the contractile vacuole system of DictyosteliumGunther Gerisch
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Cell Biol Int 26:845-52. 2002..Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man... - Host cell-dependent secretion and translocation of the LepA and LepB effectors of Legionella pneumophilaJohn Chen
Department of Microbiology, Columbia University Medical Center, New York, NY 10032, USA
Cell Microbiol 9:1660-71. 2007..In contrast, overexpression of Lep-containing hybrid proteins appears to specifically inhibit the activity of the Icm/Dot TFSS and may prevent the delivery of other effectors that are critical for intracellular multiplication... - Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideumGrant P Otto
Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St, New York, NY 10032, USA
Mol Microbiol 51:63-72. 2004..pneumophila. We conclude that macroautophagy is dispensable for L. pneumophila intracellular replication in D. discoideum... - Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cellsTill Bretschneider
Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
Curr Biol 14:1-10. 2004..The analysis of actin network dynamics requires the combination of high-resolution imaging with a specific fluorescent probe that highlights the filamentous actin structures in live cells... - Dynamics of endocytic traffic of Entamoeba histolytica revealed by confocal microscopy and flow cytometryIsaura Meza
Departamento de Biomedicina Molecular, CINVESTAV del IPN, Mexico DF, Mexico
Cell Motil Cytoskeleton 59:215-26. 2004..A striking feature of the trophozoites was the great heterogeneity of their endocytic behavior... - Legionella effectors that promote nonlytic release from protozoaJohn Chen
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA
Science 303:1358-61. 2004..Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen...