Research Topics
| E Fidelma BoydSummaryAffiliation: University of Delaware Country: USA Publications
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Publications
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine ModelW Brian Whitaker
Address correspondence to E Fidelma Boyd
Infect Immun 80:1834-45. 2012..Thus, the defect in stress tolerance and colonization in ?toxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus...- Sialic acid catabolism and transport gene clusters are lineage specific in Vibrio vulnificusJean Bernard Lubin
Microbiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom
Appl Environ Microbiol 78:3407-15. 2012..Overall, our study demonstrates that the ability to catabolize and transport sialic acid is predominately lineage specific in V. vulnificus and that the TRAP transporter is essential for sialic acid uptake... - Genomic islands are dynamic, ancient integrative elements in bacterial evolutionE Fidelma Boyd
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Trends Microbiol 17:47-53. 2009..In addition, we explore the diversity of genomic islands and their insertion sites among Gram-negative bacteria and discuss why they integrate at a limited number of tRNA genes... - Rapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi CDavid F Woods
Department of Biological Sciences, University of Delaware, Newark, DE 19716
J Clin Microbiol 46:4018-22. 2008..The technique was also adapted for a real-time PCR rapid-detection assay for both serovars Choleraesuis and Paratyphi C that complements the current procedures for Salmonella sp. isolation and serotyping... - Molecular analysis of the emergence of pandemic Vibrio parahaemolyticusE Fidelma Boyd
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
BMC Microbiol 8:110. 2008..This new highly virulent strain is now globally disseminated... - Insights into the evolution of sialic acid catabolism among bacteriaSalvador Almagro-Moreno
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
BMC Evol Biol 9:118. 2009..Sialic acids from the human host are used by a number of pathogens as an energy source. Here we explore the evolution of the genes involved in the catabolism of sialic acid... - Modulation of responses of Vibrio parahaemolyticus O3:K6 to pH and temperature stresses by growth at different salt concentrationsW Brian Whitaker
Department of Biological Sciences, University of Delaware, Newark, DE 19711, USA
Appl Environ Microbiol 76:4720-9. 2010..The effect of V. parahaemolyticus growth in 1% or 3% NaCl was examined using a cytotoxicity assay, and we found that V. parahaemolyticus grown in 1% NaCl was significantly more toxic than that grown in 3% NaCl... - Dichotomy in the evolution of pathogenicity island and bacteriophage encoded integrases from pathogenic Escherichia coli strainsMichael G Napolitano
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Infect Genet Evol 11:423-36. 2011..coli PAIs. Overall this work is the first to demonstrate a dichotomy in the evolution of integrases encoded on PAIs and phages from pathogenic E. coli suggesting that PAIs are an evolutionary distinct genetic element... - Excision dynamics of Vibrio pathogenicity island-2 from Vibrio cholerae: role of a recombination directionality factor VefASalvador Almagro-Moreno
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
BMC Microbiol 10:306. 2010..IntV2 is required for VPI-2 excision from chromosome 1, which occurs at very low levels, and formation of a non-replicative circular intermediate... - Osmoadaptation among Vibrio species and unique genomic features and physiological responses of Vibrio parahaemolyticusLynn M Naughton
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Appl Environ Microbiol 75:2802-10. 2009..We complemented E. coli MKH13, a compatible solute transporter-negative strain, with bcct2 and demonstrated uptake of betaine at high salt concentrations... - Genomic and metabolic profiling of nonulosonic acids in Vibrionaceae reveal biochemical phenotypes of allelic divergence in Vibrio vulnificusAmanda L Lewis
Department of Molecular Microbiology, Washington University, St Louis, Missouri 63110, USA
Appl Environ Microbiol 77:5782-93. 2011..Taken together, the data implicate the family Vibrionaceae as a "hot spot" of NulO evolution and suggest that these molecules may have diverse roles in environmental persistence and/or animal virulence... - Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic islandAna Luisa V Cohen
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Appl Environ Microbiol 73:5553-65. 2007..Our data suggest that lineage I may have a higher pathogenic potential and that region XII, along with other regions, may give isolates a selective advantage either in the human host or in the aquatic environment or both... - Three pathogenicity islands of Vibrio cholerae can excise from the chromosome and form circular intermediatesRonan A Murphy
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
J Bacteriol 190:636-47. 2008..These results show that all three pathogenicity islands can excise from the chromosome, which is likely a first step in their horizontal transfer... - An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosidesElizabeth L Adams
Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA
J Microbiol Methods 84:352-4. 2011..V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces... - Sialic acid catabolism confers a competitive advantage to pathogenic vibrio cholerae in the mouse intestineSalvador Almagro-Moreno
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
Infect Immun 77:3807-16. 2009..Our data indicate an important relationship between the catabolism of sialic acid and bacterial pathogenesis, stressing the relevance of the utilization of the resources found in the host's environment... - The Vibrio seventh pandemic island-II is a 26.9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43.4 kb genomic island in V. vulnificusShirley Finnan
Department of Microbiology, UCC, National University of Ireland, Cork, Ireland
Microbiology 150:4053-63. 2004..vulnificus strain, CMCP6, did not contain the 43.4 kb VVI-I; in this strain two ORFs were found between the 5' and 3' flanking genes VV10636 and VV10632, showing 100 % identity to VV0508 and VV0561, respectively, which flank VVI-I... - Mobilization of the Vibrio pathogenicity island between Vibrio cholerae isolates mediated by CP-T1 generalized transductionE Fidelma Boyd
Department of Microbiology, National University of Ireland, University College Cork, Cork, Ireland
FEMS Microbiol Lett 214:153-7. 2002..In this paper, we show that the VPI can be transferred between O1 serogroup strains, the predominant cause of epidemic cholera, via a generalized transducing phage CP-T1... - Four genomic islands that mark post-1995 pandemic Vibrio parahaemolyticus isolatesCatherine C Hurley
Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
BMC Genomics 7:104. 2006..In 1996 the first appearance of a pandemic V. parahaemolyticus clone occurred, a new O3:K6 serotype strain that has now been identified worldwide as a major cause of seafood-borne gastroenteritis... - Genomic island identification in Vibrio vulnificus reveals significant genome plasticity in this human pathogenA M Quirke
Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
Bioinformatics 22:905-10. 2006..Supplementary information: Supplementary data are available at Bioinformatics online... - Molecular evolution of Vibrio pathogenicity island-2 (VPI-2): mosaic structure among Vibrio cholerae and Vibrio mimicus natural isolatesWilliam S Jermyn
Department of Microbiology, UCC, National University of Ireland Cork, Cork, Ireland
Microbiology 151:311-22. 2005..cholerae sequences; however, on the nanH gene tree, V. mimicus clustered with V. cholerae non-O1/non-O139 isolates, suggesting horizontal transfer of this region between these species... - Evolutionary genetic analysis of the emergence of epidemic Vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profilesYvonne A O'Shea
Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
J Clin Microbiol 42:4657-71. 2004..cholerae natural isolates indicate that consecutive pandemic strains arose from a common O1 serogroup progenitor through the successive acquisition of new virulence regions... - Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environmentShirley Finnan
Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
J Clin Microbiol 42:5783-92. 2004..Our data demonstrate the significant role horizontal gene transfer and recombination, together with gene loss, play in the evolution of this important human pathogen... - Crystal structure of VC1805, a conserved hypothetical protein from a Vibrio cholerae pathogenicity island, reveals homology to human p32Md Arif Sheikh
Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife, KY16 9ST, United Kingdom
Proteins 71:1563-71. 2008 - Investigation of a global collection of nontyphoidal Salmonella of various serotypes cultured between 1953 and 2004 for the presence of class 1 integronsBrenda P Murphy
Veterinary Department, Cork County Council, County Hall, Cork, Ireland
FEMS Microbiol Lett 266:170-6. 2007..A Salmonella Wien isolate possessing a complete class 1 integron with a dfrA5-ereA2 gene arrangement within the variable region was characterized... - The genomic code: inferring Vibrionaceae niche specializationF Jerry Reen
F. Jerry Reen and Salvador Almagro Moreno are at the Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland
Nat Rev Microbiol 4:697-704. 2006..The existence of complete genome sequences for closely related species from varied aquatic niches makes this group an excellent case study for genome comparison... - Evolutionary and functional analyses of variants of the toxin-coregulated pilus protein TcpA from toxigenic Vibrio cholerae non-O1/non-O139 serogroup isolatesE Fidelma Boyd
Department of Microbiology, National University of Ireland, University College Cork, Cork, Ireland
Microbiology 148:1655-66. 2002..All the V. cholerae non-O1 serogroup isolates tested were capable of colonizing the suckling mouse small intestine, suggesting that the different TcpA variants could function as colonization factors...