Research Topics
| Katherine A BorkovichSummaryAffiliation: University of California Country: USA Publications
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Publications
Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organismKatherine A Borkovich
Department of Plant Pathology, University of California, Riverside, California 92521, USA
Microbiol Mol Biol Rev 68:1-108. 2004..The group of unshared genes includes potential new targets for antifungals as well as loci implicated in human and plant physiology and disease...- The predicted G-protein-coupled receptor GPR-1 is required for female sexual development in the multicellular fungus Neurospora crassaSvetlana Krystofova
Department of Plant Pathology, University of California, 1415 Boyce Hall, 900 University Ave, Riverside, 92521, USA
Eukaryot Cell 5:1503-16. 2006..The lack of ostioles in Deltagpr-1 and Deltabek-1 mutants has an undesirable effect on the ability to spread progeny (ascospores) by the normal ejection mechanism and would severely compromise the fitness of these strains in nature... - RIC8 is a guanine-nucleotide exchange factor for Galpha subunits that regulates growth and development in Neurospora crassaSara J Wright
Program in Biochemistry and Molecular Biology, Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA
Genetics 189:165-76. 2011..RIC8 acts as a GEF for GNA-1 and GNA-3 in vitro, with the strongest effect on GNA-3. Our results support a role for RIC8 in regulating GNA-1 and GNA-3 in Neurospora... - Mitogen-activated protein kinase cascade required for regulation of development and secondary metabolism in Neurospora crassaGyungsoon Park
Department of Plant Pathology and Microbiology, 1415 Boyce Hall, University of California, Riverside, 900 University Avenue, Riverside, CA 92521, USA
Eukaryot Cell 7:2113-22. 2008..Tyrosinase is an enzyme that catalyzes production of the secondary metabolite l-DOPA melanin. These results implicate the MAK-1 pathway in regulation of development and secondary metabolism in filamentous fungi... - Global analysis of serine-threonine protein kinase genes in Neurospora crassaGyungsoon Park
Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
Eukaryot Cell 10:1553-64. 2011..Finally, we demonstrated allelism between the S/T kinase gene NCU00406 and velvet (vel), encoding a p21-activated protein kinase (PAK) gene important for asexual and sexual growth and development in Neurospora... - GPR-4 is a predicted G-protein-coupled receptor required for carbon source-dependent asexual growth and development in Neurospora crassaLiande Li
Department of Plant Pathology, 1415 Boyce Hall, 900 University Avenue, University of California, Riverside, Riverside, CA 92521, USA
Eukaryot Cell 5:1287-300. 2006..crassa. GPR-4-related GPCRs are present in the genomes of several filamentous ascomycete fungal pathogens, raising the possibility that a similar pathway regulates carbon sensing in these organisms... - Pheromones are essential for male fertility and sufficient to direct chemotropic polarized growth of trichogynes during mating in Neurospora crassaHyojeong Kim
Department of Plant Pathology, 1415 Boyce Hall, 900 University Avenue, University of California, Riverside, CA 92521, USA
Eukaryot Cell 5:544-54. 2006..Furthermore, a self-attraction signal can be transmitted within a strain that expresses a pheromone and its cognate receptor... - High-throughput production of gene replacement mutants in Neurospora crassaGyungsoon Park
Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA
Methods Mol Biol 722:179-89. 2011..In addition, development of software programs for primer design and restriction enzyme selection facilitated the high-throughput aspects of the overall protocol... - Heterotrimeric G protein signaling in filamentous fungiLiande Li
Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521, USA
Annu Rev Microbiol 61:423-52. 2007..This review provides a comparative analysis of G protein pathways in several filamentous species, with discussion of both unifying themes and important unique signaling paradigms... - Analysis of mitogen-activated protein kinase phosphorylation in response to stimulation of histidine kinase signaling pathways in NeurosporaCarol A Jones
Department of Plant Pathology and Microbiology, University of California, Riverside, California, USA
Methods Enzymol 471:319-34. 2010..The protein extraction method presented may also be used to concentrate cellular proteins for additional applications, such as metabolic labeling or analysis of other posttranslational modifications... - The response regulator RRG-1 functions upstream of a mitogen-activated protein kinase pathway impacting asexual development, female fertility, osmotic stress, and fungicide resistance in Neurospora crassaCarol A Jones
Department of Plant Pathology and Microbiology, and Program in Biochemistry and Molecular Biology, University of California, Riverside, Riverside, CA 92521, USA
Mol Biol Cell 18:2123-36. 2007..The data indicate that RRG-1 controls vegetative cell integrity, hyperosmotic sensitivity, fungicide resistance, and protoperithecial development through regulation of the OS-4/OS-5/OS-2 MAPK pathway... - The heterotrimeric G-protein subunits GNG-1 and GNB-1 form a Gbetagamma dimer required for normal female fertility, asexual development, and galpha protein levels in Neurospora crassaSvetlana Krystofova
Department of Plant Pathology, University of California Riverside, Riverside, CA 92521, USA
Eukaryot Cell 4:365-78. 2005..Direct evidence for a physical association between GNB-1 and GNG-1 in vivo was provided by coimmunoprecipitation... - A pheromone receptor gene, pre-1, is essential for mating type-specific directional growth and fusion of trichogynes and female fertility in Neurospora crassaHyojeong Kim
Department of Plant Pathology, University of California, 2338 Webber Hall, 900 University Avenue, Riverside, CA 92521, USA
Mol Microbiol 52:1781-98. 2004..We propose that PRE-1 is a pheromone receptor coupled to GNA-1 that is essential for the mating of mat A strains as females, consistent with a role in launching the pheromone response pathway in N. crassa... - Use of 1H nuclear magnetic resonance to measure intracellular metabolite levels during growth and asexual sporulation in Neurospora crassaJames D Kim
Department of Plant Pathology and Microbiology, University of California, 900 University Avenue, Riverside, CA 92521, USA
Eukaryot Cell 10:820-31. 2011..However, analysis of individual metabolites revealed differences in wild-type and ?gna-3 strains cultured under conditions of low and high sucrose... - The fungal opsin gene nop-1 is negatively-regulated by a component of the blue light sensing pathway and influences conidiation-specific gene expression in Neurospora crassaJennifer A Bieszke
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 6431 Fannin Street, JFB 1 765, Houston, TX 77030, USA
Curr Genet 52:149-57. 2007..The results suggest that NOP-1 directly or indirectly modulates carotenogenesis and repression of conidiation-specific gene expression in N. crassa... - A G-protein beta subunit required for sexual and vegetative development and maintenance of normal G alpha protein levels in Neurospora crassaQi Yang
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
Eukaryot Cell 1:378-90. 2002..In contrast, many other delta gnb-1 phenotypes, including female sterility and defective conidiation, can be explained by altered levels of the three N. crassa G alpha proteins... - The genome sequence of the filamentous fungus Neurospora crassaJames E Galagan
Whitehead Institute Center for Genome Research, 320 Charles Street, Cambridge, Massachusetts 02141, USA
Nature 422:859-68. 2003..Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes... - Shared and independent roles for a Galpha(i) protein and adenylyl cyclase in regulating development and stress responses in Neurospora crassaF Douglas Ivey
Department of Microbiology and Molecular Genetics, University of Texas--Houston Medical School, Houston, Texas 77030, USA
Eukaryot Cell 1:634-42. 2002..Taken together, the results suggest that in general, GNA-1 and CR-1 regulate N. crassa growth and development using parallel pathways, while thermotolerance is largely dependent on cAMP... - Circadian rhythmicity mediated by temporal regulation of the activity of p38 MAPKMichael W Vitalini
Center for Biological Clocks Research, Faculty of Genetics, and Program for the Biology of Filamentous Fungi, 3258 TAMU Department of Biology, Texas A and M University, College Station, TX 77843, USA
Proc Natl Acad Sci U S A 104:18223-8. 2007..crassa circadian clock allows anticipation and preparation for hyperosmotic stress and desiccation that begin at sunrise. These results suggest a conserved role for MAPK pathways in circadian rhythmicity... - A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factorsHildur V Colot
Department of Genetics, Dartmouth Medical School, HB7400, Hanover, NH 03755, USA
Proc Natl Acad Sci U S A 103:10352-7. 2006..Overall, the methodology, which achieves high-throughput gene disruption at an efficiency >90% in this filamentous fungus, promises to be applicable to other eukaryotic organisms that have a low frequency of homologous recombination... - Severe impairment of growth and differentiation in a Neurospora crassa mutant lacking all heterotrimeric G alpha proteinsAnn M Kays
Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, Texas 77031, USA
Genetics 166:1229-40. 2004..These studies indicate that although G alpha subunits are not essential for viability in filamentous fungi, their loss results in an organism that cannot effectively forage for nutrients or undergo asexual or sexual reproduction... - Enabling a community to dissect an organism: overview of the Neurospora functional genomics projectJay C Dunlap
Department of Genetics, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
Adv Genet 57:49-96. 2007..In addition, these studies have driven the assembly of an SNP map presently populated by nearly 300 markers that will greatly accelerate the positional cloning of genes...