Sashwati Roy

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. ncbi Laser microdissection and capture of pure cardiomyocytes and fibroblasts from infarcted heart regions: perceived hyperoxia induces p21 in peri-infarct myocytes
    Donald E Kuhn
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 292:H1245-53. 2007
  2. ncbi Topical oxygen therapy induces vascular endothelial growth factor expression and improves closure of clinically presented chronic wounds
    Gayle M Gordillo
    The Comprehensive Wound Center, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Clin Exp Pharmacol Physiol 35:957-64. 2008
  3. ncbi miRNA in wound inflammation and angiogenesis
    Sashwati Roy
    Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Microcirculation 19:224-32. 2012
  4. ncbi Redox regulation of the VEGF signaling path and tissue vascularization: Hydrogen peroxide, the common link between physical exercise and cutaneous wound healing
    Sashwati Roy
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 44:180-92. 2008
  5. ncbi Transcriptome-wide analysis of blood vessels laser captured from human skin and chronic wound-edge tissue
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 104:14472-7. 2007
  6. ncbi Particulate ?-glucan induces TNF-? production in wound macrophages via a redox-sensitive NF-??-dependent pathway
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Wound Repair Regen 19:411-9. 2011
  7. ncbi MiRNA in innate immune responses: novel players in wound inflammation
    Sashwati Roy
    Comprehensive Wound Center and Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Physiol Genomics 43:557-65. 2011
  8. ncbi Dermal wound healing properties of redox-active grape seed proanthocyanidins
    Savita Khanna
    Department of Surgery, Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 33:1089-96. 2002
  9. ncbi Anti-angiogenic property of edible berries
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, 512 Heart and Lung Research Institute, Ohio State University Medical Center, 473 W. 12th Avenue, Columbus, OH 43210, USA
    Free Radic Res 36:1023-31. 2002
  10. ncbi Fra-2 mediates oxygen-sensitive induction of transforming growth factor beta in cardiac fibroblasts
    Sashwati Roy
    Department of Surgery, The Ohio State University Medical Center, 513 Davis Heart and Lung Research Institute, 473 West 12th Avenue, Columbus, OH 43210, USA
    Cardiovasc Res 87:647-55. 2010

Research Grants

  1. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2009
  2. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2010
  3. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2010

Collaborators

Detail Information

Publications87

  1. ncbi Laser microdissection and capture of pure cardiomyocytes and fibroblasts from infarcted heart regions: perceived hyperoxia induces p21 in peri-infarct myocytes
    Donald E Kuhn
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 292:H1245-53. 2007
    ..This work presents the first account of an analytical technique that applies the LMPC technology to study myocardial remodeling with a cell-type specific resolution...
  2. ncbi Topical oxygen therapy induces vascular endothelial growth factor expression and improves closure of clinically presented chronic wounds
    Gayle M Gordillo
    The Comprehensive Wound Center, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Clin Exp Pharmacol Physiol 35:957-64. 2008
    ..Treatment with TO is associated with an induction of VEGF expression in wound edge tissue and an improvement in wound size...
  3. ncbi miRNA in wound inflammation and angiogenesis
    Sashwati Roy
    Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Microcirculation 19:224-32. 2012
    ..Understanding the significance of miRs in wound inflammation and angiogenesis may help design therapeutic strategies for management of chronic nonhealing wounds...
  4. ncbi Redox regulation of the VEGF signaling path and tissue vascularization: Hydrogen peroxide, the common link between physical exercise and cutaneous wound healing
    Sashwati Roy
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 44:180-92. 2008
    ..Numerous antioxidants exhibit antiangiogenic properties. Current evidence lends firm credence to the hypothesis that low-level endogenous H(2)O(2) supports vascular growth...
  5. ncbi Transcriptome-wide analysis of blood vessels laser captured from human skin and chronic wound-edge tissue
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 104:14472-7. 2007
    ..Sets of genes with known vascular functions but never connected to wound healing were identified to be differentially expressed in wound-derived blood vessels paving the way for innovative clinically relevant hypotheses...
  6. ncbi Particulate ?-glucan induces TNF-? production in wound macrophages via a redox-sensitive NF-??-dependent pathway
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Wound Repair Regen 19:411-9. 2011
    ..Activation of wound macrophages by Glyc101 represents one of the potential mechanisms by which this ?-glucan may benefit chronic wounds where inefficient inflammatory response is one of the underlying causes of impaired healing...
  7. ncbi MiRNA in innate immune responses: novel players in wound inflammation
    Sashwati Roy
    Comprehensive Wound Center and Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Physiol Genomics 43:557-65. 2011
    ..Such treatment would help establish new paradigms highlighting a central role of miRs in the understanding and management of dysregulated inflammation as noted in conjunction with chronic wounds...
  8. ncbi Dermal wound healing properties of redox-active grape seed proanthocyanidins
    Savita Khanna
    Department of Surgery, Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 33:1089-96. 2002
    ..In summary, our current study provides firm evidence to support that topical application of GSPE represents a feasible and productive approach to support dermal wound healing...
  9. ncbi Anti-angiogenic property of edible berries
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, 512 Heart and Lung Research Institute, Ohio State University Medical Center, 473 W. 12th Avenue, Columbus, OH 43210, USA
    Free Radic Res 36:1023-31. 2002
    ..Matrigel assay using human dermal microvascular endothelial cells showed that edible berries impair angiogenesis...
  10. ncbi Fra-2 mediates oxygen-sensitive induction of transforming growth factor beta in cardiac fibroblasts
    Sashwati Roy
    Department of Surgery, The Ohio State University Medical Center, 513 Davis Heart and Lung Research Institute, 473 West 12th Avenue, Columbus, OH 43210, USA
    Cardiovasc Res 87:647-55. 2010
    ....
  11. ncbi Transcriptome of primary adipocytes from obese women in response to a novel hydroxycitric acid-based dietary supplement
    Sashwati Roy
    Laboratory of Molecular Medicine and the Microarray Core Facility, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    DNA Cell Biol 26:627-39. 2007
    ..Although HCA-SX seems to be conditionally effective in weight management in experimental animals and in humans, its mechanism of action remains unclear...
  12. ncbi Oxygen: from the benefits of inducing VEGF expression to managing the risk of hyperbaric stress
    Viren Patel
    Laboratory of Molecular Medicine, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Antioxid Redox Signal 7:1377-87. 2005
    ..This is of special concern for people with inadequate intake of dietary antioxidants. Nutritional antioxidant supplementation may offset HBO-induced oxidative stress...
  13. ncbi Oxidant-induced vascular endothelial growth factor expression in human keratinocytes and cutaneous wound healing
    Chandan K Sen
    Laboratory of Molecular Medicine, Dorothy M Davis Heart and Lung Research Institute, Department of Surgery Center for Minimally Invasive Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Biol Chem 277:33284-90. 2002
    ..Taken together, the results of this study indicate that wound healing is subject to redox control...
  14. ncbi Improved function of diabetic wound-site macrophages and accelerated wound closure in response to oral supplementation of a fermented papaya preparation
    Eric Collard
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Antioxid Redox Signal 13:599-606. 2010
    ..Given that FPP has a long track record of safe human consumption, testing of the beneficial effects of FPP on diabetic wound-related outcomes in a clinical setting is warranted...
  15. ncbi Nanomolar vitamin E alpha-tocotrienol inhibits glutamate-induced activation of phospholipase A2 and causes neuroprotection
    Savita Khanna
    Department of Surgery, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    J Neurochem 112:1249-60. 2010
    ..This work presents first evidence recognizing glutamate-induced changes in cPLA(2) as a novel mechanism responsible for neuroprotection observed in response to nanomolar concentrations of TCT...
  16. ncbi Neuroprotective properties of the natural vitamin E alpha-tocotrienol
    Savita Khanna
    Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio, USA
    Stroke 36:2258-64. 2005
    ..CONCLUSIONS: The natural vitamin E, TCT, acts on key molecular checkpoints to protect against glutamate- and stroke-induced neurodegeneration...
  17. ncbi Transcriptome of the subcutaneous adipose tissue in response to oral supplementation of type 2 Leprdb obese diabetic mice with niacin-bound chromium
    Cameron Rink
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Physiol Genomics 27:370-9. 2006
    ..Further studies testing the molecular basis of NBC function and long-term outcomes are warranted...
  18. ncbi Characterization of the potent neuroprotective properties of the natural vitamin E alpha-tocotrienol
    Savita Khanna
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Colombus, Ohio 43210, USA
    J Neurochem 98:1474-86. 2006
    ..Oral supplementation of alpha-tocotrienol to humans results in a peak plasma concentration of 3 microm. Thus, oral alpha-tocotrienol may be neuroprotective by antioxidant-independent as well as antioxidant-dependent mechanisms...
  19. ncbi Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds
    Sabyasachi Biswas
    Department of Surgery, Ohio State University Medical Center, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 107:6976-81. 2010
    ....
  20. ncbi MicroRNA expression in response to murine myocardial infarction: miR-21 regulates fibroblast metalloprotease-2 via phosphatase and tensin homologue
    Sashwati Roy
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 West 12th Avenue, Columbus, OH 43210, USA
    Cardiovasc Res 82:21-9. 2009
    ..For the first time, we sought to test how myocardial ischaemia-reperfusion (IR) changes miR expression...
  21. ncbi Assessment of wound-site redox environment and the significance of Rac2 in cutaneous healing
    Navdeep Ojha
    Department of Surgery, Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 44:682-91. 2008
    ..Taken together, the findings of this study support that the wound site is rich in oxidants. Rac2 significantly contributes to oxidant production at the wound site and supports the healing process...
  22. ncbi Natural vitamin E alpha-tocotrienol: retention in vital organs in response to long-term oral supplementation and withdrawal
    Viren Patel
    Laboratory of Molecular Medicine, Department of Surgery, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Res 40:763-71. 2006
    ....
  23. ncbi Anti-angiogenic property of edible berry in a model of hemangioma
    Mustafa Atalay
    Laboratory of Molecular Medicine, Department of Surgery, 512 Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 W. 12th Avenue, Columbus 43210, USA
    FEBS Lett 544:252-7. 2003
    ..The current results provide the first in vivo evidence substantiating the anti-angiogenic property of edible berries...
  24. ncbi Oxygen-sensitive outcomes and gene expression in acute ischemic stroke
    Cameron Rink
    Department of Surgery, The Ohio State University Medical Center, 473 W 12th Avenue, Columbus, OH 43210, USA
    J Cereb Blood Flow Metab 30:1275-87. 2010
    ..The findings provide key information relevant to understanding oxygen-dependent molecular mechanisms in the AIS-affected brain...
  25. ncbi Dermal wound healing is subject to redox control
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Comprehensive Wound Center, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Mol Ther 13:211-20. 2006
    ..Taken together, this work presents the first in vivo evidence indicating that strategies to influence the redox environment of the wound site may have a bearing on healing outcomes...
  26. ncbi Characterization of the acute temporal changes in excisional murine cutaneous wound inflammation by screening of the wound-edge transcriptome
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Physiol Genomics 34:162-84. 2008
    ..This study provides a digested database that may serve as a valuable reference tool to develop novel hypotheses aiming to elucidate the biology of cutaneous wound healing comprehensively...
  27. ncbi Characterization of a preclinical model of chronic ischemic wound
    Sashwati Roy
    Comprehensive Wound Center, Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Physiol Genomics 37:211-24. 2009
    ..This study provides a thorough database that may serve as a valuable reference tool to develop novel hypotheses aiming to elucidate the biology of ischemic chronic wounds in a preclinical setting...
  28. ncbi Characterization of the structural and functional changes in the myocardium following focal ischemia-reperfusion injury
    Navdeep Ojha
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH, USA
    Am J Physiol Heart Circ Physiol 294:H2435-43. 2008
    ..Myocardial remodeling following I/R includes secondary myocyte death followed by the loss of cardiac function over time...
  29. ncbi Transcriptome analysis of the ischemia-reperfused remodeling myocardium: temporal changes in inflammation and extracellular matrix
    Sashwati Roy
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio, USA
    Physiol Genomics 25:364-74. 2006
    ..Taken together, this work presents the first evidence gained from the use of DNA microarrays to understand the molecular mechanisms implicated in the early and late inflammatory phases of the I-R heart...
  30. ncbi Evidence for the involvement of miRNA in redox regulated angiogenic response of human microvascular endothelial cells
    Shani Shilo
    Comprehensive Wound Center, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Arterioscler Thromb Vasc Biol 28:471-7. 2008
    ..A Dicer knockdown approach was used to test the significance of miRNA in regulating the redox state and angiogenic response of human microvascular endothelial cells (HMECs)...
  31. ncbi P21waf1/cip1/sdi1 as a central regulator of inducible smooth muscle actin expression and differentiation of cardiac fibroblasts to myofibroblasts
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Mol Biol Cell 18:4837-46. 2007
    ..This observation establishes p21 as a central regulator of reperfusion-induced phenotypic switch of CFs to myofibroblasts...
  32. ncbi Regulation of vascular responses to inflammation: inducible matrix metalloproteinase-3 expression in human microvascular endothelial cells is sensitive to antiinflammatory Boswellia
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Antioxid Redox Signal 8:653-60. 2006
    ..In all experiments, both in vitro and in vivo, BE30% was more effective than BE3%. In sum, this work lends support to our previous report that BE has potent antiinflammatory properties both in vitro as well as in vivo...
  33. ncbi Oral tocotrienols are transported to human tissues and delay the progression of the model for end-stage liver disease score in patients
    Viren Patel
    Department of Internal Medicine, Ohio State University Medical Center, Columbus, OH, USA
    J Nutr 142:513-9. 2012
    ..The findings of this study, in the context of the current literature, lay the foundation for Phase II clinical trials testing the efficacy of TE against stroke and end-stage liver disease in humans...
  34. ncbi Nox-4-dependent nuclear H2O2 drives DNA oxidation resulting in 8-OHdG as urinary biomarker and hemangioendothelioma formation
    Gayle Gordillo
    Division of Plastic Surgery, Department of Surgery, Davis Heart Lung Research Institute, The Ohio State University, Columbus, Ohio 43212, USA
    Antioxid Redox Signal 12:933-43. 2010
    ..The current state of information connects nox-4 to MCP-1 to form a major axis of control that regulates the fate of hemangioendothelioma development in vivo...
  35. ncbi Human genome screen to identify the genetic basis of the anti-inflammatory effects of Boswellia in microvascular endothelial cells
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    DNA Cell Biol 24:244-55. 2005
    ..These findings warrant further research aimed at identifying the signaling mechanisms by which BE exerts its anti-inflammatory effects...
  36. ncbi Macrophage dysfunction impairs resolution of inflammation in the wounds of diabetic mice
    Savita Khanna
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio, United States of America
    PLoS ONE 5:e9539. 2010
    ..At the wound-site, efficient dead cell clearance (efferocytosis) is a pre-requisite for the timely resolution of inflammation and successful healing...
  37. ncbi Body weight and abdominal fat gene expression profile in response to a novel hydroxycitric acid-based dietary supplement
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Gene Expr 11:251-62. 2004
    ..Functional characterization of HCA-SX-sensitive genes revealed that upregulation of genes encoding serotonin receptors represent a distinct effect of dietary HCA-SX supplementation...
  38. ncbi Tocotrienol: the natural vitamin E to defend the nervous system?
    Chandan K Sen
    Davis Heart and Lung Research Institute, 473 West 12th Avenue, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Ann N Y Acad Sci 1031:127-42. 2004
    ..Dietary supplementation studies have established that tocotrienol, fed orally, does reach the brain. The current findings point towards tocotrienol as a potent neuroprotective form of natural vitamin E...
  39. ncbi Delivery of orally supplemented alpha-tocotrienol to vital organs of rats and tocopherol-transport protein deficient mice
    Savita Khanna
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Free Radic Biol Med 39:1310-9. 2005
    ..Examination of whether alpha-TCT may benefit humans suffering from neurological disorders because of congenital TTP deficiency is warranted...
  40. ncbi Molecular basis of vitamin E action: tocotrienol modulates 12-lipoxygenase, a key mediator of glutamate-induced neurodegeneration
    Savita Khanna
    Laboratory of Molecular Medicine, Department of Surgery, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Biol Chem 278:43508-15. 2003
    ..These findings lend further support to alpha-tocotrienol as a potent neuroprotective form of vitamin E...
  41. ncbi Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation
    Sashwati Roy
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Wound Repair Regen 19:753-66. 2011
    ..Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers...
  42. ncbi Micromanaging vascular biology: tiny microRNAs play big band
    Chandan K Sen
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Vasc Res 46:527-40. 2009
    ..Prudent targeting of non-coding genes such as miRNAs, which in turn regulates large sets of coding genes, holds promise in gene therapy. Recent developments in miRNA biology offer lucrative opportunities to manage vascular health...
  43. ncbi Neuroprotective and antiinflammatory properties of a novel demethylated curcuminoid
    Savita Khanna
    Departments of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Antioxid Redox Signal 11:449-68. 2009
    ..Taken together, DC exhibited promising neuroprotective and antiinflammatory properties that must be characterized in vivo...
  44. ncbi Detection of macrophages via paramagnetic vesicles incorporating oxidatively tailored cholesterol ester: an approach for atherosclerosis imaging
    Andrei Maiseyeu
    Davis Heart and Lung Research Institute, Room 110, 473 W 12th Avenue, Columbus, OH 43210 1252, USA
    Nanomedicine (Lond) 5:1341-56. 2010
    ..In this article we describe the synthesis of biocompatible, paramagnetic, fluorescent phosphatidylserine vesicles containing cholesterol ester with a free carboxylic acid function and its use for targeted imaging of macrophages...
  45. ncbi Oxygen sensing by primary cardiac fibroblasts: a key role of p21(Waf1/Cip1/Sdi1)
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus 43210, USA
    Circ Res 92:264-71. 2003
    ..CFs deficient in p21 were resistant to such O2 sensitivity. This study raises the vital broad-based issue of controlling ambient O2 during the culture of primary cells isolated from organs...
  46. ncbi Perceived hyperoxia: oxygen-induced remodeling of the reoxygenated heart
    Chandan K Sen
    Laboratory of Molecular Medicine, 512 Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, 473 W 12th Avenue, Columbus, 43210, USA
    Cardiovasc Res 71:280-8. 2006
    ..Understanding of the underlying mechanisms of this and other myocardial healing responses should prove to be instrumental in developing productive therapeutic approaches to mend the infarcted heart...
  47. ncbi Characterization of perceived hyperoxia in isolated primary cardiac fibroblasts and in the reoxygenated heart
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 West 12th Avenue, Columbus, OH 43210, USA
    J Biol Chem 278:47129-35. 2003
    ..Furthermore, the first evidence demonstrating activation of perceived hyperoxia sensitive genes in the cardiac I-R tissue is presented...
  48. ncbi Prevalence of obstructive sleep apnea in patients with chronic wounds
    Brian T Patt
    Sleep Heart Program, Critical Care, and Sleep Medicine, The Ohio State University, Columbus, OH 43210, USA
    J Clin Sleep Med 6:541-4. 2010
    ..We hypothesized that OSA would have more prevalence in patients of a wound center than the general middle-aged population...
  49. ncbi Regulation of c-Src activity in glutamate-induced neurodegeneration
    Savita Khanna
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Biol Chem 282:23482-90. 2007
    ..Findings of this study present a new paradigm that addresses the regulation of c-Src under neurodegenerative conditions...
  50. ncbi Dermal excisional wound healing in pigs following treatment with topically applied pure oxygen
    Richard B Fries
    Laboratory of Molecular Medicine, Dorothy M. Davis Heart and Lung Research Institute and Comprehensive Wound Center, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Mutat Res 579:172-81. 2005
    ..Topically applied pure oxygen has the potential of benefiting some wound types. Further studies testing the potential of topical oxygen in pre-clinical and clinical settings are warranted...
  51. ncbi Laser microdissection and pressure-catapulting technique to study gene expression in the reoxygenated myocardium
    Donald E Kuhn
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, 473 W. 12th Avenue, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 290:H2625-32. 2006
    ..This work reports a sophisticated yet rapid approach to measurement of relative gene expressions from tissue elements captured from spatially resolved microscopic regions in the heart with micrometer-level precision...
  52. ncbi Oxygen-sensitive reset of hypoxia-inducible factor transactivation response: prolyl hydroxylases tune the biological normoxic set point
    Savita Khanna
    Laboratory of Molecular Medicine, Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, 43210, USA
    Free Radic Biol Med 40:2147-54. 2006
    ..siRNA dependent knockdown of PHD expression revealed that O(2)-sensitive regulation of PHD may contribute to tuning the normoxic set point in biological cells...
  53. ncbi Reactive oxygen species and EGR-1 gene expression in surgical postoperative peritoneal adhesions
    Sashwati Roy
    Department of Surgery, Center for Minimally Invasive Surgery, Laboratory of Molecular Medicine, Davis Heart and Lung Research Institute, The Ohio State University, 473 W 12th Avenue, Columbus, OH 73210, USA
    World J Surg 28:316-20. 2004
    ..The newly developed rodent model of adhesion may present a useful approach to reproducibly and objectively study molecular mechanisms underlying the dynamic process of de novo adhesion tissue formation...
  54. ncbi Hyperglycemic oxoaldehyde, glyoxal, causes barrier dysfunction, cytoskeletal alterations, and inhibition of angiogenesis in vascular endothelial cells: aminoguanidine protection
    Sean M Sliman
    Lipid Signaling and Lipidomics and Vasculotoxicity Laboratory, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH 43210, USA
    Mol Cell Biochem 333:9-26. 2010
    ....
  55. ncbi Minimally invasive neuroradiologic model of preclinical transient middle cerebral artery occlusion in canines
    Cameron Rink
    Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 105:14100-5. 2008
    ..This work reports the successful development of a powerful preclinical model of stroke that lends itself to the study of biologic mechanisms as well as to testing experimental therapeutics...
  56. ncbi Oxygenation state as a driver of myofibroblast differentiation and wound contraction: hypoxia impairs wound closure
    Chandan K Sen
    Comprehensive Wound Center, Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Invest Dermatol 130:2701-3. 2010
    ..They present compelling evidence indicating tissue hypoxia conflicts with wound closure. We are reminded that correcting wound tissue hypoxia is critical for the tissue's response to other therapeutic interventions...
  57. ncbi A key angiogenic role of monocyte chemoattractant protein-1 in hemangioendothelioma proliferation
    Gayle M Gordillo
    Division of Plastic Surgery, The Ohio State University Medical Center, 410 W 10th Ave, Columbus, OH 43210, USA
    Am J Physiol Cell Physiol 287:C866-73. 2004
    ..This study has produced the first in vivo evidence of a complete response for any neoplasm, specifically a vascular proliferative lesion, to anti-MCP-1 therapy in animals with intact immune systems...
  58. ncbi Copper-induced vascular endothelial growth factor expression and wound healing
    Chandan K Sen
    Laboratory of Molecular Medicine, Department of Surgery, 512 Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Am J Physiol Heart Circ Physiol 282:H1821-7. 2002
    ..Copper-sensitive pathways regulate key mediators of wound healing such as angiogenesis and extracellular matrix remodeling. Copper-based therapeutics represents a feasible approach to promote dermal wound healing...
  59. ncbi miR-200b targets Ets-1 and is down-regulated by hypoxia to induce angiogenic response of endothelial cells
    Yuk Cheung Chan
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Biol Chem 286:2047-56. 2011
    ..This study provides the first evidence that hypoxia-sensitive miR-200b is involved in induction of angiogenesis via directly targeting Ets-1 in HMECs...
  60. ncbi Hemangioma model for in vivo angiogenesis: inducible oxidative stress and MCP-1 expression in EOMA cells
    Gayle M Gordillo
    Department of Surgery, Laboratory of Molecular Medicine, Davis Heart and Lung Research Institute, Ohio State University, Columbus, Ohio 43210, USA
    Methods Enzymol 352:422-32. 2002
  61. ncbi Tocotrienols: the emerging face of natural vitamin E
    Chandan K Sen
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Vitam Horm 76:203-61. 2007
    ..The current state of knowledge warrants strategic investment into the lesser known forms of vitamin E. This will enable prudent selection of the appropriate vitamin E molecule for studies addressing a specific health need...
  62. ncbi Wound site neutrophil transcriptome in response to psychological stress in young men
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Gene Expr 12:273-87. 2005
    ..Further effort to gain a more comprehensive understanding of the functional significance of such behavior-genome interaction is warranted...
  63. ncbi MicroRNA in cutaneous wound healing: a new paradigm
    Shani Shilo
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio, USA
    DNA Cell Biol 26:227-37. 2007
    ..If tested positive, this hypothesis is likely to lead to completely novel diagnostic and therapeutic strategies for the treatment of problem wounds...
  64. ncbi Redox signals in wound healing
    Chandan K Sen
    Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Biochim Biophys Acta 1780:1348-61. 2008
    ..The objective of this review is to describe our current understanding of how redox-sensitive processes may drive dermal tissue repair...
  65. ncbi Natural vitamin E ?-tocotrienol protects against ischemic stroke by induction of multidrug resistance-associated protein 1
    Han A Park
    Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, Ohio, USA
    Stroke 42:2308-14. 2011
    ..Multidrug resistance-associated protein 1 (MRP1), a key mediator of intracellular oxidized glutathione efflux from neural cells, may therefore possess neuroprotective functions...
  66. ncbi Tocotrienols: Vitamin E beyond tocopherols
    Chandan K Sen
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Life Sci 78:2088-98. 2006
    ..The current state of knowledge warrants strategic investment into the lesser known forms of vitamin E. This will enable prudent selection of the appropriate vitamin E molecule for studies addressing a specific need...
  67. ncbi Human skin wounds: a major and snowballing threat to public health and the economy
    Chandan K Sen
    Department of Surgery, The Ohio State University Comprehensive Wound Center, Columbus, Ohio 43210, USA
    Wound Repair Regen 17:763-71. 2009
    ..The immense economic and social impact of wounds in our society calls for allocation of a higher level of attention and resources to understand biological mechanisms underlying cutaneous wound complications...
  68. ncbi Endothelial dysfunction in the microcirculation of patients with obstructive sleep apnea
    Brian T Patt
    The Ohio State University Columbus, USA
    Am J Respir Crit Care Med 182:1540-5. 2010
    ..Obstructive sleep apnea (OSA) is a risk factor for cardiovascular disease. We hypothesized that patients with OSA and no cardiovascular disease have oxidant-related microcirculatory endothelial dysfunction...
  69. ncbi Algorithms for mapping of mRNA targets for microRNA
    Ilya Ioshikhes
    Department of Biomedical Informatics, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    DNA Cell Biol 26:265-72. 2007
    ..Strategies for improvement of the current bioinformatics tools and effective approaches for biological validation are discussed...
  70. ncbi Tocotrienols in health and disease: the other half of the natural vitamin E family
    Chandan K Sen
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, United States
    Mol Aspects Med 28:692-728. 2007
    ..The current state of knowledge warrants strategic investment into investigating the lesser known forms of vitamin E...
  71. ncbi Perceived hyperoxia: oxygen-regulated signal transduction pathways in the heart
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Methods Enzymol 381:133-9. 2004
  72. ncbi Oxygen, oxidants, and antioxidants in wound healing: an emerging paradigm
    Chandan K Sen
    Laboratory of Molecular Medicine, Dorothy M Davis Heart and Lung Research Institute, Department of Surgery CMIS, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Ann N Y Acad Sci 957:239-49. 2002
    ..Strategies to manipulate the redox environment in the wound are likely to be of outstanding significance in wound healing...
  73. ncbi Restraint stress alters lung gene expression in an experimental influenza A viral infection
    Andrea Engler
    Laboratory of Neuroendocrine Immunology, Section of Oral Biology, College of Dentistry, The Ohio State University Health Sciences Center, Columbus, OH 43210, USA
    J Neuroimmunol 162:103-11. 2005
    ....
  74. ncbi Vitamin E sensitive genes in the developing rat fetal brain: a high-density oligonucleotide microarray analysis
    Sashwati Roy
    Laboratory of Molecular Medicine, Department of Surgery and Molecular and Cellular Biochemistry, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 W. 12th Avenue, Columbus 43210, USA
    FEBS Lett 530:17-23. 2002
    ..The enrichment is more in fetal brain tissue. Using a GeneChip microarray expression profiling approach we have identified a specific set of vitamin E sensitive genes in the developing rat fetal brain...
  75. ncbi Microarray analysis of gene expression: considerations in data mining and statistical treatment
    Joseph S Verducci
    Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University, Columbus, Ohio, USA
    Physiol Genomics 25:355-63. 2006
    ....
  76. ncbi DNA microarray technology in nutraceutical and food safety
    Yiwen Liu-Stratton
    Laboratory of Molecular Medicine and DNA Microarray and Genetics Facility, Department of Surgery, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 W. 12th Avenue, Columbus, OH 43210, USA
    Toxicol Lett 150:29-42. 2004
    ..Optimization of this technology to specifically address food safety is a vast resource that remains to be mined. Efforts to develop diagnostic custom arrays and simplified bioinformatics tools for field use are warranted...
  77. ncbi Simultaneous detection of tocopherols and tocotrienols in biological samples using HPLC-coulometric electrode array
    Sashwati Roy
    Departments of Surgery and Molecular and Cellular Biochemistry, Laboratory of Molecular Medicine, Davis Heart and Lung Research Institute, Ohio State University, Columbus, Ohio 43210, USA
    Methods Enzymol 352:326-32. 2002
  78. ncbi Aerobically derived lactate stimulates revascularization and tissue repair via redox mechanisms
    Thomas K Hunt
    Wound Healing Laboratory, University of California San Francisco, San Francisco, California 94143 0522, USA
    Antioxid Redox Signal 9:1115-24. 2007
    ..Lactate and oxygen together stimulate angiogenesis and matrix deposition...
  79. ncbi Flavonoids differentially regulate IFN gamma-induced ICAM-1 expression in human keratinocytes: molecular mechanisms of action
    Toshinori Bito
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
    FEBS Lett 520:145-52. 2002
    ....
  80. ncbi Postprandial levels of the natural vitamin E tocotrienol in human circulation
    Pramod Khosla
    Department of Nutrition and Food Science, Wayne State University, Detroit, Michigan, USA
    Antioxid Redox Signal 8:1059-68. 2006
    ..Tocotrienols were detected in the blood plasma and all lipoprotein subfractions studied postprandially...
  81. ncbi cDNA microarray screening in food safety
    Sashwati Roy
    Laboratory of Molecular Medicine and DNA Microarray and Genetics Facility, Dorothy M. Davis Heart and Lung Research Institute, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Toxicology 221:128-33. 2006
    ..Its values lie in being able to provide complimentary molecular insight when employed in addition to traditional tests for food safety, as part of a more comprehensive battery of tests...
  82. ncbi miRNA: licensed to kill the messenger
    Chandan K Sen
    DNA Cell Biol 26:193-4. 2007
    ..Having acknowledged the potential risk, we have to recognize that new knowledge about the functional roles of miRNA is revolutionizing cell biology and will have a major impact on biomedical research imminently...
  83. ncbi Harnessing hypoxic adaptation to prevent, treat, and repair stroke
    Rajiv R Ratan
    Winifred Masterson Burke Medical Research Institute, White Plains, NY 10605, USA
    J Mol Med (Berl) 85:1331-8. 2007
    ..The breadth and depth of this homeostatic program offers a hopeful alternative to the current pessimism towards stroke therapeutics...
  84. ncbi Relief from a heavy heart: redox-sensitive NF-kappaB as a therapeutic target in managing cardiac hypertrophy
    Chandan K Sen
    Am J Physiol Heart Circ Physiol 289:H17-9. 2005
  85. ncbi Glutamate-induced c-Src activation in neuronal cells
    Savita Khanna
    Departments of Surgery and Molecular and Cellular Biochemistry, Laboratory of Molecular Medicine, Davis Heart and Lung Research Institute, Ohio State University, Columbus, Ohio 43210, USA
    Methods Enzymol 352:191-8. 2002
  86. ncbi Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals
    Natalie B Schweitzer
    Department of Zoology, Miami University, Oxford, OH 45056, USA
    Life Sci 77:2246-61. 2005
    ..In conclusion, a total of 44 CV genes were expressed differently in SED compared to PA and EX; and SED showed more physiological evidence of CVD...
  87. ncbi Functional genomics: high-density oligonucleotide arrays
    Sashwati Roy
    Laboratory of Molecular Medicine, Departments of Surgery and Molecular and Cellular Biochemistry, Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Methods Enzymol 353:487-97. 2002

Research Grants4

  1. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2009
    ..Successful completion of the project will offer novel therapeutic opportunities to treat chronic inflammation that is commonly associated with problem wounds. ..
  2. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2010
    ..Successful completion of the project will offer novel therapeutic opportunities to treat chronic inflammation that is commonly associated with problem wounds. ..
  3. Mechanisms underlying impaired diabetic wound healing
    Sashwati Roy; Fiscal Year: 2010
    ..Successful completion of the project will offer novel therapeutic opportunities to treat chronic inflammation that is commonly associated with problem wounds. ..