Research Topics
Genomes and Genes | Jennifer L JohnsonSummaryAffiliation: The Scripps Research Institute Country: USA Publications
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Publications
A systematic approach to understand the mechanism of action of the bisthiazolium compound T4 on the human malaria parasite, Plasmodium falciparumKarine G Le Roch
Department of Cell Biology and Neuroscience, University of California, Riverside, 900 University Avenue, Riverside, CA 92521, USA
BMC Genomics 9:513. 2008..Here we have employed transcriptome and proteome profiling analyses to characterize the global response of P. falciparum to T4 during the intraerythrocytic cycle of this parasite...
Gene transfer and expression in human neutrophils. The phox homology domain of p47phox translocates to the plasma membrane but not to the membrane of mature phagosomesJennifer L Johnson
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, USA
BMC Immunol 7:28. 2006..Consequently, exogenous gene expression in neutrophils is challenging. We report here the transfection of genes and expression of active proteins in human primary peripheral neutrophils using nucleofection...
Rab27a and Rab27b regulate neutrophil azurophilic granule exocytosis and NADPH oxidase activity by independent mechanismsJennifer L Johnson
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Traffic 11:533-47. 2010..Exocytosis of secretory vesicles in Rab27-deficient neutrophils was functional, suggesting that Rab27 GTPases selectively control the exocytosis of neutrophil granules...
Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytesDaniela B Munafo
Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Biochem J 402:229-39. 2007..Our results suggest that the granules implicated in cargo release towards the surrounding milieu are molecularly and mechanistically different from those involved in their release towards the phagolysosome...
Cross-talk between IRAK-4 and the NADPH oxidaseSandrine Pacquelet
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Biochem J 403:451-61. 2007..We conclude that IRAK-4 phosphorylates p47phox and regulates NADPH oxidase activation after LPS stimulation...
The Rab27a effectors JFC1/Slp1 and Munc13-4 regulate exocytosis of neutrophil granulesAgnieszka A Brzezinska
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Traffic 9:2151-64. 2008..However, mobilization of CD11b was not affected in Munc13-4-deficient neutrophils, indicating that secretory defects in these cells are limited to a selective group of exocytosable organelles...
Signalling mechanisms for Toll-like receptor-activated neutrophil exocytosis: key roles for interleukin-1-receptor-associated kinase-4 and phosphatidylinositol 3-kinase but not Toll/IL-1 receptor (TIR) domain-containing adaptor inducing IFN-beta (TRIF)Agnieszka A Brzezinska
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA
Immunology 127:386-97. 2009..Our results identified IRAK-4, p38MAPK and PI3K as important regulatory components with different roles in the signalling pathways that control Toll-like receptor ligand-triggered neutrophil exocytosis...
Munc13-4 restricts motility of Rab27a-expressing vesicles to facilitate lipopolysaccharide-induced priming of exocytosis in neutrophilsJennifer L Johnson
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 286:5647-56. 2011....
Increased survival and reduced neutrophil infiltration of the liver in Rab27a- but not Munc13-4-deficient mice in lipopolysaccharide-induced systemic inflammationJennifer L Johnson
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Infect Immun 79:3607-18. 2011....
The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphataseJennifer L Johnson
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Biochem J 391:699-710. 2005..In conclusion, we present evidence that JFC1 differentially regulates the secretion of PSAP and PSA, and that Rab27a and PI3K play a central role in the exocytosis of prostate-specific markers...
DNase I inhibits a late phase of reactive oxygen species production in neutrophilsDaniela B Munafo
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
J Innate Immun 1:527-42. 2009..Altogether, our data suggest that inhibition of ROS production by microorganism-derived DNase would contribute to their ability to evade killing...
Akt regulates the subcellular localization of the Rab27a-binding protein JFC1 by phosphorylationJennifer L Johnson
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Traffic 6:667-81. 2005..Our results suggest that Akt regulates JFC1/Slp1 function by phosphorylation and may have implications on Rab27a-containing vesicle secretion...
The C2A domain of JFC1 binds to 3'-phosphorylated phosphoinositides and directs plasma membrane association in living cellsSergio D Catz
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:11652-7. 2002..We also show that the association of the C2A domain to the membrane is modulated by calcium. From these results we analyze possible mechanisms for the role of JFC1 in cellular trafficking...
