Research Topics
| David A SteinSummaryCountry: USA Publications
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Publications
Inhibition of RNA virus infections with peptide-conjugated morpholino oligomersDavid A Stein
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA
Curr Pharm Des 14:2619-34. 2008....- Treatment of AG129 mice with antisense morpholino oligomers increases survival time following challenge with dengue 2 virusDavid A Stein
AVI BioPharma Inc, 4575 SW Research Way, Corvallis, OR 97333, USA
J Antimicrob Chemother 62:555-65. 2008..To determine the antiviral activity of phosphorodiamidate morpholino oligomers (PMO) and peptide-conjugated PMO (PPMO) in AG129 mice infected with dengue 2 virus (DENV-2)... - Inhibition of HSV-1 ocular infection with morpholino oligomers targeting ICP0 and ICP27Megan Moerdyk-Schauwecker
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Antiviral Res 84:131-41. 2009..5-50% compared to controls. This study demonstrates that topically applied PPMO holds promise as an antiviral drug candidate against HSV-1 ocular infection... - Reduction of herpes simplex virus type-2 replication in cell cultures and in rodent models with peptide-conjugated morpholino oligomersKathleen Eide
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
Antivir Ther 15:1141-9. 2010..Peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs) are single-stranded DNA analogues that enter cells readily and can reduce target gene expression through steric blockage of complementary messenger RNA (mRNA)... - Inhibition of multiple subtypes of influenza A virus in cell cultures with morpholino oligomersQing Ge
AVI BioPharma Inc, 4575 SW Research Way, Corvallis, OR 97333, USA
Antimicrob Agents Chemother 50:3724-33. 2006..High efficacy by two of the P-PMO against multiple FLUAV subtypes suggests that these oligomers represent a broad-spectrum therapeutic approach against a high percentage of known FLUAV strains... - Nucleic acid-based inhibition of flavivirus infectionsDavid A Stein
AVI BioPharma Inc, Corvallis, Oregon 97333, USA
Front Biosci 13:1385-95. 2008..The goal of this review is to summarize the findings of many of the significant reports that have appeared on the topic of antisense-mediated strategies for the development of antiviral therapy for flaviviruses... - Arginine-rich peptide conjugation to morpholino oligomers: effects on antisense activity and specificityMichelle H Nelson
AVI BioPharma, Inc, Corvallis, Oregon 97333, USA
Bioconjug Chem 16:959-66. 2005..Additionally, it is shown that the enzymatic stability of an L- or unnatural peptide used for PMO conjugation affects the antisense properties of the resulting compound... - Inhibition of influenza A H3N8 virus infections in mice by morpholino oligomersChristopher Lupfer
Genetics Program, College of Agricultural Science, Oregon State University, Corvallis, OR 97331, USA
Arch Virol 153:929-37. 2008..These data indicate that PPMOs targeting highly conserved regions of FLUAV are promising novel therapeutic candidates... - Vesivirus viremia and seroprevalence in humansAlvin W Smith
Department of Biomedical Sciences, College of Veterinary Medicine, Laboratory for Calicivirus Studies, Oregon State University, Corvallis, Oregon 97331, USA
J Med Virol 78:693-701. 2006..These results justify further investigation of an association between Vesivirus infection and illness in humans... - A novel antisense inhibitor of MMP-9 attenuates angiogenesis, human prostate cancer cell invasion and tumorigenicityCarla A London
AVI BioPharma Inc, 4575 SW Research Way, Suite 200, Corvallis, Oregon 97333, USA
Cancer Gene Ther 10:823-32. 2003..These data establish the feasibility of developing a site-directed, nontoxic antisense therapeutic agent for inhibiting local invasion and metastasis... - Antisense treatment of caliciviridae: an emerging disease agent of animals and humansAlvin W Smith
Oregon State University, College of Veterinary Medicine, Corvallis 97331, USA
Curr Opin Mol Ther 4:177-84. 2002..Antisense technology using phosphorodiamidate morpholino oligomers shows promise in overcoming these current diagnostic and therapeutic problems inherent with newly emerging viral diseases... - Peptide-conjugated morpholino oligomers inhibit porcine reproductive and respiratory syndrome virus replicationDeendayal Patel
Molecular Virology Laboratory, VA MD Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
Antiviral Res 77:95-107. 2008..5UP2 and 5HP inhibited virus replication of 10 other strains of PRRSV. Results from this study suggest potential applications of PPMO for PRRS control... - Inhibition of dengue virus serotypes 1 to 4 in vero cell cultures with morpholino oligomersRichard M Kinney
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado, USA
J Virol 79:5116-28. 2005..The 5'SL and 3'CS P4-PMOs did not suppress the replication of West Nile virus NY99 in Vero cells. These data indicate that further evaluation of the 5'SL and 3'CS compounds as potential DEN virus therapeutics is warranted... - Inhibition of alphavirus infection in cell culture and in mice with antisense morpholino oligomersSlobodan Paessler
Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
Virology 376:357-70. 2008..Levels of virus in tissue samples correlated with animal survival. Uninfected mice suffered no apparent ill-effects from PPMO treatment. Thus, PPMO appear promising as candidates for therapeutic development against alphaviruses... - In vitro resistance selection and in vivo efficacy of morpholino oligomers against West Nile virusTia S Deas
Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
Antimicrob Agents Chemother 51:2470-82. 2007..The results suggest that a modification of the peptide conjugate composition to reduce its toxicity yet maintain its ability to effectively deliver PMO into cells may improve PMO-mediated therapy... - Antiviral effects of antisense morpholino oligomers in murine coronavirus infection modelsRenaud Burrer
The Scripps Research Institute, Department of Molecular and Integrative Neurosciences, Mail Drop SP30 2020, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
J Virol 81:5637-48. 2007..However, the strong antiviral effect observed suggests that with further development, P-PMO may provide an effective therapeutic approach against a broad range of coronavirus infections... - VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in miceSven Enterlein
Department of Virology, Philipps University Marburg, Hans Meerwein str 3, 35043 Marburg, Germany
Antimicrob Agents Chemother 50:984-93. 2006..Together, these data suggest that antisense PMO and P-PMO have the potential to control EBOV infection and are promising therapeutic candidates... - A morpholino oligomer targeting highly conserved internal ribosome entry site sequence is able to inhibit multiple species of picornavirusJeffrey K Stone
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143, USA
Antimicrob Agents Chemother 52:1970-81. 2008..These results suggest that EnteroX may have broad therapeutic potential against entero- and rhinoviruses... - Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomersBenjamin W Neuman
The Scripps Research Institute, Division of Virology, Department of Neuropharmacology, La Jolla, CA 92037, USA
J Virol 79:9665-76. 2005..Those partially resistant viruses grew more slowly and formed smaller plaques than wild-type SARS-CoV. These results suggest PMO compounds have powerful therapeutic and investigative potential toward coronavirus infection... - Inhibition of flavivirus infections by antisense oligomers specifically suppressing viral translation and RNA replicationTia S Deas
Wadsworth Center, New York State Department of Health, 120 New Scotland Ave, Albany, NY 12208, USA
J Virol 79:4599-609. 2005.... - Antisense morpholino-oligomers directed against the 5' end of the genome inhibit coronavirus proliferation and growthBenjamin W Neuman
The Scripps Research Institute, Department of Neuropharmacology, Division of Virology, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
J Virol 78:5891-9. 2004..These results suggest that this composition of antisense compound has therapeutic potential for control of coronavirus infection... - Morpholino oligomers targeting the PB1 and NP genes enhance the survival of mice infected with highly pathogenic influenza A H7N7 virusGülsah Gabriel
Institute of Virology, Philipps University Marburg, Germany
J Gen Virol 89:939-48. 2008..These data suggest that PPMO provide a useful reagent to investigate influenza virus molecular biology and may constitute a therapeutic strategy against highly pathogenic influenza viruses... - West Nile virus genome cyclization and RNA replication require two pairs of long-distance RNA interactionsBo Zhang
Wadsworth Center, New York State Department of Health, Albany, New York 12201, USA
Virology 373:1-13. 2008..Therefore, the 5'UAR/3'UAR pairing may release the 3' end of viral genome (as a template) during the initiation of minus-strand RNA synthesis... - Inhibition of dengue virus translation and RNA synthesis by a morpholino oligomer targeted to the top of the terminal 3' stem-loop structureKatherine Lynn Holden
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, 140 Warren Hall, Berkeley, CA 94720-7360, USA
Virology 344:439-52. 2006..These results show that the 3'CS and the 3'SL domains regulate DEN translation and RNA synthesis and further demonstrate that P-PMOs are potentially useful as antiviral agents... - Inhibition of foot-and-mouth disease virus infections in cell cultures with antisense morpholino oligomersAriel Vagnozzi
Plum Island Animal Disease Center, U S Department of Agriculture Agricultural Research Service NAA, Greenport, NY 11944 0848, USA
J Virol 81:11669-80. 2007..The results indicate that PPMOs have potential for treating FMDV infections and that they also represent useful tools for studying picornaviral translation and evolution... - Inhibition and escape of SARS-CoV treated with antisense morpholino oligomersBenjamin W Neuman
The Scripps Research Institute, La Jolla, California, USA
Adv Exp Med Biol 581:567-71. 2006 - Antiviral activity of morpholino oligomers designed to block various aspects of Equine arteritis virus amplification in cell cultureErwin van den Born
Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, LUMC P4-26, PO Box 9600, 2300 RC Leiden, The Netherlands
J Gen Virol 86:3081-90. 2005..The data suggest that these compounds could be useful as reagents for exploring the molecular mechanics of nidovirus translation and have anti-EAV potential at relatively low concentrations... - Gene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomersKelly L Warfield
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA
PLoS Pathog 2:e1. 2006..The work described here may contribute to development of designer, "druggable" countermeasures for filoviruses and other microbial pathogens... - Inhibition of replication and transcription activator and latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus by morpholino oligomersYan Jin Zhang
Center for Pediatric Research, Eastern Virginia Medical School, Norfolk, VA 23510, USA
Antiviral Res 73:12-23. 2007..These results suggest that RP1 P-PMO can specifically block KSHV replication, and further study is warranted... - Suppression of porcine reproductive and respiratory syndrome virus replication by morpholino antisense oligomersYan Jin Zhang
Center for Pediatric Research, Eastern Virginia Medical School, Norfolk, VA 23510, USA
Vet Microbiol 117:117-29. 2006..Cell viability assays detected no cytotoxicity of 5UP1 within the concentration-range of this study. These results indicate that P-PMO 5UP1 has potential as an anti-PRRSV agent... - Inhibition of coxsackievirus B3 in cell cultures and in mice by peptide-conjugated morpholino oligomers targeting the internal ribosome entry siteJi Yuan
The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, B.C, Canada
J Virol 80:11510-9. 2006..Thus, PPMO-6 provided potent inhibition of CVB3 amplification both in cell cultures and in vivo and appears worthy of further evaluation as a candidate for clinical development...