Research Topics
| Jianghong RaoSummaryAffiliation: Stanford University Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Self-illuminating quantum dot conjugates for in vivo imagingMin-Kyung So
Molecular Imaging Program, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305-5484, USA
Nat Biotechnol 24:339-43. 2006..Compared with existing quantum dots, self-illuminating quantum dot conjugates have greatly enhanced sensitivity in small animal imaging, with an in vivo signal-to-background ratio of > 10(3) for 5 pmol of conjugate...- Fluorescence imaging in vivo: recent advancesJianghong Rao
Molecular Imaging Program at Stanford, Department of Radiology and Bio X Program, Cancer Biology Program, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305 5484, USA
Curr Opin Biotechnol 18:17-25. 2007..Further emerging developments are aiming to achieve high-resolution, multimodality and lifetime-based in vivo fluorescence imaging... - HaloTag protein-mediated specific labeling of living cells with quantum dotsMin Kyung So
Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, P093, Mail code 5484, Stanford, CA 94305, USA
Biochem Biophys Res Commun 374:419-23. 2008..Live cell fluorescence imaging indicates that the labeling is specific and takes place at the cell surface. This HaloTag protein-mediated cell labeling method should facilitate the application of quantum dots for live cell imaging... - Quantum dot imaging for embryonic stem cellsShuan Lin
Molecular Imaging Program at Stanford MIPS and Bio X Program, Department of Radiology, Stanford University, Stanford, CA 94305, USA
BMC Biotechnol 7:67. 2007..In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled with Qtracker delivered quantum dots (QDs)... - Visualizing RNA splicing in vivoGayatri Gowrishankar
Molecular Imaging Program at Stanford, Department of Radiology and Bio X Program, Cancer Biology Program, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305 5484, USA
Mol Biosyst 3:301-7. 2007.... - Shedding light on tumors using nanoparticlesJianghong Rao
Department of Radiology and Bio X Program, Stanford University School of Medicine, Stanford, California 94305 5484, USA
ACS Nano 2:1984-6. 2008..This versatile nanoplatform promises more efficient delivery of payloads to tumors for improving cancer detection and treatment... - Quantum dot bioconjugates for in vitro diagnostics & in vivo imagingYun Xing
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine Stanford, CA 94305, USA
Cancer Biomark 4:307-19. 2008..In addition, we will discuss the making of a new class of QDs--the self-illuminating QDs and their in vivo imaging and sensing applications. We will conclude with the issues and perspectives on QDs as in vivo imaging probes... - Immobilizing reporters for molecular imaging of the extracellular microenvironment in living animalsZuyong Xia
Molecular Imaging Program at Stanford, Department of Radiology and Bio X Program, Stanford University, California 94035 5484, United States
ACS Chem Biol 6:1117-26. 2011..Our results show that the in vivo immobilization of reporters can be used as a general strategy for probing the local extracellular microenvironment... - Imaging target mRNA and siRNA-mediated gene silencing in vivo with ribozyme-based reportersMin Kyung So
Molecular Imaging Program at Stanford, Department of Radiology, Biophysics, Cancer Biology Programs, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305 5484, USA
Chembiochem 9:2682-91. 2008..This new ribozyme-based RNA reporter system should open up new avenues for in vivo RNA imaging and direct imaging of siRNA inhibition... - Particle size, surface coating, and PEGylation influence the biodistribution of quantum dots in living miceMeike L Schipper
Molecular Imaging Program at Stanford MIPS, Department of Radiology, Bio X Program, Stanford University, 318 Campus Drive, Palo Alto, CA 94305 5427, USA
Small 5:126-34. 2009..Renal excretion of small QDs and slowing of RES clearance by PEGylation or peptide surface coating are encouraging steps toward the use of modified QDs for imaging living subjects... - Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugationZuyong Xia
Biophysics Program, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305 5484, USA
Anal Chem 80:8649-55. 2008..The suitability of these nanosensors for a multiplex protease assay has also been shown... - Improved QD-BRET conjugates for detection and imagingYun Xing
Molecular Imaging Program at Stanford MIPS, Department of Radiology, School of Medicine, Stanford University, Stanford, CA 94305, USA
Biochem Biophys Res Commun 372:388-94. 2008..Stable QD-BRET probes should further facilitate their applications for both in vitro testing as well as in vivo cell tracking studies... - Real-time imaging of Rab5 activity using a prequenched biosensorKe Zhan
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, California 94305 5484, United States
ACS Chem Biol 6:692-9. 2011..This novel method should enable imaging of the biological process in which Rab5 activity is regulated in various cellular systems... - Creating self-illuminating quantum dot conjugatesMin-Kyung So
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305-5484, USA
Nat Protoc 1:1160-4. 2006.... - Biosensing and imaging based on bioluminescence resonance energy transferZuyong Xia
Department of Radiology and Bio X Program, Stanford University School of Medicine, Stanford, CA 94305 5484, USA
Curr Opin Biotechnol 20:37-44. 2009..Future development of new BRET acceptors should further expand the multiplexing capability of BRET and improve its applicability and sensitivity for in vivo imaging applications... - A bioluminogenic substrate for in vivo imaging of beta-lactamase activityHequan Yao
Molecular Imaging Program at Stanford, Department of Radiology, Biophysics, Cancer Biology Programs, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
Angew Chem Int Ed Engl 46:7031-4. 2007 - HaloTag protein-mediated site-specific conjugation of bioluminescent proteins to quantum dotsYan Zhang
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
Angew Chem Int Ed Engl 45:4936-40. 2006 - CNOB/ChrR6, a new prodrug enzyme cancer chemotherapySteve H Thorne
Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
Mol Cancer Ther 8:333-41. 2009..This feature may simplify exploration of barriers to the penetration of MCHB in tumors and their amelioration... - A self-assembled quantum dot probe for detecting beta-lactamase activityChenjie Xu
Biophysics, Cancer Biology, and Molecular Imaging Programs, Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Biochem Biophys Res Commun 344:931-5. 2006..Our final quantum dot probe, assembled with QD605 and 1:1 mixture of biotin and a Cy5-labeled lactam, can be activated by 32microg/mL of beta-lactamase with 4-fold increase in the fluorescence emission... - Detection of mRNA in mammalian cells with a split ribozyme reporterSumitaka Hasegawa
Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
Chembiochem 7:925-8. 2006 - Combining SELEX screening and rational design to develop light-up fluorophore-RNA aptamer pairs for RNA taggingJungjoon Lee
Department of Chemistry, Stanford University School of Medicine, 1210 Welch Road, Stanford, California 94305 5484, USA
ACS Chem Biol 5:1065-74. 2010..This study demonstrates the value of combining in vitro SELEX and E. coli fluorescence screening with rational modifications in discovering and optimizing new fluorogen-RNA aptamer labeling pairs... - Quantum dot/bioluminescence resonance energy transfer based highly sensitive detection of proteasesHequan Yao
Molecular Imaging Program at Stanford, Department of Radiology, Biophysics, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
Angew Chem Int Ed Engl 46:4346-9. 2007 - Protease-modulated cellular uptake of quantum dotsYan Zhang
Biophysics, Cancer Biology, and Molecular Imaging Programs, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305-5484, USA
Nano Lett 6:1988-92. 2006..This enzyme-modulated cellular uptake of QDs may be applied to other nanoparticles for biological imaging and selective drug delivery into tumor cells... - A biocompatible condensation reaction for controlled assembly of nanostructures in living cellsGaolin Liang
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305 5484, USA
Nat Chem 2:54-60. 2010..This intracellular condensation process enabled the imaging of the proteolytic activity of furin... - Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugatesYoung Pil Kim
Molecular Imaging Program at Stanford, Department of Radiology and Chemistry, Stanford University, Stanford, CA 94305 5484, USA
Chem Commun (Camb) 46:76-8. 2010..The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage... - In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substratesAnca Dragulescu-Andrasi
Department of Radiology, Stanford University School of Medicine, Stanford, California 94305, USA
Bioconjug Chem 20:1660-6. 2009..Direct imaging of furin activity may facilitate the study of furin function in tumorigenicity and the discovery of new drugs for furin-targeted cancer therapy... - Semiconductor quantum dots for biosensing and in vivo imagingYun Xing
Molecular Imaging Program at Stanford MIPS, Department of Radiology, Stanford University, Stanford, CA 94305, USA
IEEE Trans Nanobioscience 8:4-12. 2009..We conclude with a discussion on the issues and perspectives on QDs as biosensing probes and in vivo imaging agents... - Chemical labeling of protein in living cellsAnca Dragulescu-Andrasi
Molecular Imaging Program at Stanford, Department of Radiology and Bio-X Program, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
Chembiochem 8:1099-101. 2007 - microPET-based biodistribution of quantum dots in living miceMeike L Schipper
Departments of Radiology and Bioengineering, Bio X Program, Molecular Imaging Program at Stanford MIPS, Stanford University, East 150 Clark Center, Palo Alto, CA 94305, USA
J Nucl Med 48:1511-8. 2007..This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice... - Modulating the splicing activity of Tetrahymena ribozyme via RNA self-assemblySumitaka Hasegawa
Biophysics Program, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA
FEBS Lett 580:1592-6. 2006..The splicing efficiency was dependent on the length of appending nucleotides... - Near-infrared light emitting luciferase via biomineralizationNan Ma
Molecular Imaging Program at Stanford, Stanford University, 1201 Welch Road, Stanford, California 94305, USA
J Am Chem Soc 132:6884-5. 2010..This is the first study to form dual functional bioinorganic hybrid nanostructures via active enzyme-templated synthesis of inorganic nanomaterials... - Strategies for in vivo imaging of enzyme activity: an overview and recent advancesAndrew Razgulin
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 1201 Welch Road, California 94305 5484, USA
Chem Soc Rev 40:4186-216. 2011..Recent advances in combining multiple modalities to imaging enzyme activity in living subjects are also highlighted (255 references)... - Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophoresHsiao lu D Lee
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Am Chem Soc 132:15099-101. 2010.... - How molecular imaging is speeding up antiangiogenic drug developmentWeibo Cai
The Molecular Imaging Program at Stanford, Department of Radiology and Bio-X Program, Stanford University School of Medicine, 1201 Welch Road, P095, Stanford, CA 94305-5484, USA
Mol Cancer Ther 5:2624-33. 2006..Molecular imaging has enormous potential in improving the efficiency of the drug development process, including the specific area of antiangiogenic drugs... - Single-cell detection of trans-splicing ribozyme in vivo activitySumitaka Hasegawa
Department of Radiology and Bio-X Program, Molecular Imaging Program at Stanford, Stanford University, 300 Pasteur Drive, Stanford, California 94305-5344, USA
J Am Chem Soc 126:7158-9. 2004.... - Cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase activityBengang Xing
Department of Radiology, Biophysics, Bio-X, Cancer Biology, and Molecular Imaging Programs at Stanford, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5344, USA
J Am Chem Soc 127:4158-9. 2005..This new type of fluorogenic probe may also be applied to image gene expression in living animals... - Imaging Tetrahymena ribozyme splicing activity in single live mammalian cellsSumitaka Hasegawa
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095-1770, USA
Proc Natl Acad Sci U S A 100:14892-6. 2003..Our work has provided a new reporter system that allows high-throughput screening with flow cytometry of single living mammalian cells for a direct and facile in vivo selection of desired ribozyme variants... - Novel fluorogenic substrates for imaging beta-lactamase gene expressionWenzhong Gao
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA
J Am Chem Soc 125:11146-7. 2003..These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor...
Research Grants
- A Unified Reporter Gene for Multi-Modality ImagingJianghong Rao; Fiscal Year: 2005..abstract_text> ..
- QD-BRET nanosensors for protease detection and imagingJianghong Rao; Fiscal Year: 2009..A sophisticated understanding of the differences of enzyme activity between tumor and normal tissues in living subjects will advance our understanding of cancer metastasis and help develop antimetastasis therapy. ..
- QD-BRET nanosensors for protease detection and imagingJianghong Rao; Fiscal Year: 2010..A sophisticated understanding of the differences of enzyme activity between tumor and normal tissues in living subjects will advance our understanding of cancer metastasis and help develop antimetastasis therapy. ..