Research TopicsSpecies | MICHELE CALOSSummaryAffiliation: Stanford University Country: USA Publications
Research Grants
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Detail Information
Publications
Phage integrases for the construction and manipulation of transgenic mammalsRoger P Hollis
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
Reprod Biol Endocrinol 1:79. 2003..The results reviewed here introduce these bacteriophage integrases as tools for site-specific modification of the genome for the creation and manipulation of transgenic mammals...- Therapeutic applications of the PhiC31 integrase systemChristopher L Chavez
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
Curr Gene Ther 11:375-81. 2011..We conclude that gene therapy strategies utilizing ?C31 integrase offer great promise for the development of treatments in the future... - Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31Amy C Groth
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
Genetics 166:1775-82. 2004..These experiments demonstrate the potential for precise genetic engineering of the Drosophila genome with the phiC31 integrase system and will likely benefit research in Drosophila and other insects... - The phiC31 integrase system for gene therapyMichele P Calos
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
Curr Gene Ther 6:633-45. 2006..Ongoing and planned improvements to the phage integrase system are discussed. We conclude that gene therapy strategies using phiC31 integrase and its derivatives offer great promise for success in the near term... - Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genesDaniel S Ginsburg
Department of Genetics Stanford University School of Medicine Stanford, California 94305, USA
Adv Genet 54:179-87. 2005..Development of phiC31 integrase-based vectors is still underway, but it has already been shown to provide long-term expression through site-specific integration... - Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage phiC31 integrase after nonviral DNA deliveryJoylette L Portlock
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Hum Gene Ther 17:871-6. 2006..These results suggest the possible utility of the phiC31 integrase system to treat ischemic disease... - PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit jointsAnnahita Keravala
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA
J Gene Med 8:1008-17. 2006.... - A diversity of serine phage integrases mediate site-specific recombination in mammalian cellsAnnahita Keravala
Department of Genetics, M-334, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5120, USA
Mol Genet Genomics 276:135-46. 2006..The other integrases did not mediate integration at genomic sequences at a frequency above background. These site-specific integrases represent valuable new tools for manipulating eukaryotic genomes... - Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmidMatthew P Fish
Department of Developmental Biology and Howard Hughes Medical Institute, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305 5120, USA
Nat Protoc 2:2325-31. 2007..The whole procedure, from injection to established transgenic stocks, can be completed in three generations (approximately 1 month) and can be adapted for other types of transgenesis and mRNA injections in Drosophila... - Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integraseAnnahita Keravala
Department of Genetics, Stanford University School of Medicine, M 334, 300 Pasteur Drive, Stanford, CA 94305 5120, United States
J Neurosci Methods 173:299-305. 2008..Our results demonstrate that the phiC31 integrase system produces stable transgene expression in adult mNPCs and their progeny and may be useful in strategies for combating neurodegenerative disorders... - Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integrationCarmen Bertoni
Department of Neurology, Stanford University School of Medicine, Stanford, CA 94305-5235, USA
Proc Natl Acad Sci U S A 103:419-24. 2006..These data demonstrate the importance of both the level and distribution of dystrophin expression to achieve therapeutic efficacy, and that the efficacy can be enhanced by targeted plasmid integration... - phiC31 integrase confers genomic integration and long-term transgene expression in rat retinaThomas W Chalberg
Department of Genetics, Stanford University School of Medicine, CA 94305, USA
Invest Ophthalmol Vis Sci 46:2140-6. 2005..These findings suggest that phiC31 integrase may be a simple and effective tool for nonviral long-term gene transfer in the eye... - Phage TP901-1 site-specific integrase functions in human cellsStephanie M Stoll
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA
J Bacteriol 184:3657-63. 2002..The TP901-1 phage integrase thus represents a new reagent for manipulating DNA in living mammalian cells... - Stable nonviral genetic correction of inherited human skin diseaseSusana Ortiz-Urda
VA Palo Alto Healthcare System and the Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA
Nat Med 8:1166-70. 2002..These findings establish a practical approach to nonviral genetic correction of severe human genetic disorders requiring stable genomic integration of large DNA sequences... - PhiC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosaSusana Ortiz-Urda
VA Palo Alto Healthcare System and Program in Epithelial Biology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA
Hum Gene Ther 14:923-8. 2003..Furthermore, corrected JEB tissue restored hemidesmosome formation and abolished histologic evidence of subepidermal blistering. These findings provide an approach to durable nonviral correction of JEB... - Phage integrases: biology and applicationsAmy C Groth
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA
J Mol Biol 335:667-78. 2004..Directed evolution can be used to increase further the affinity of an integrase for a particular native sequence, opening up additional applications for genomic modification... - Site-specific chromosomal integration mediated by phiC31 integraseAnnahita Keravala
Department of Genetics, Stanford University School of Medicine, Stanford, CA
Methods Mol Biol 435:165-73. 2008..This integration system based on phiC31 integrase supplies a simple method to obtain repeated integration at the same chromosomal site in mammalian cells... - Extrachromosomal plasmid vectors for gene therapyStephanie M Stoll
Department of Genetics, Stanford University School of Medicine, CA 94305-5120, USA
Curr Opin Mol Ther 4:299-305. 2002..This review will discuss the advantages of extrachromosomal DNA as a gene therapy vector, highlighting recent advances and successes in its use in vivo... - Integration specificity of phage phiC31 integrase in the human genomeThomas W Chalberg
Department of Genetics, Stanford University School of Medicine, Stanford, CA 95305-5120, USA
J Mol Biol 357:28-48. 2006..An analysis of the safety of integration sites in terms of proximity to cancer genes suggested minimal cancer risk. We conclude that integration systems derived from phiC31 integrase have great potential utility... - Factoring nonviral gene therapy into a cure for hemophilia AVanessa Gabrovsky
Stanford University School of Medicine, Department of Genetics, Stanford, CA 94305 5120, USA
Curr Opin Mol Ther 10:464-70. 2008..New types of nonviral strategies, such as DNA integrating vectors, and the success of several nonviral animal studies, suggest that nonviral gene therapy has curative potential and justifies its clinical development... - Site-specific genomic integration produces therapeutic Factor IX levels in miceEric C Olivares
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Nat Biotechnol 20:1124-8. 2002..Integration was documented at two pseudo-attP sites, native sequences with partial identity to attP, with one site highly predominant. This study demonstrates in vivo gene transfer in an animal by site-specific genomic integration... - Mutational derivatives of PhiC31 integrase with increased efficiency and specificityAnnahita Keravala
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5120, USA
Mol Ther 17:112-20. 2009..These mutant integrases are useful for gene therapy and genome modification, and they demonstrate the feasibility of engineering phiC31 integrase toward more desirable properties... - Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinomaLauren E Woodard
Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America
PLoS ONE 5:e11367. 2010..However, chromosomal aberrations have been associated with phiC31 integrase expression in tissue culture, leading to questions about safety... - Epstein-Barr virus vectors provide prolonged robust factor IX expression in miceChristopher R Sclimenti
Department of Genetics, Stanford University School of Medicine, California 94305, USA
Biotechnol Prog 19:144-51. 2003..These results underline the crucial importance of appropriate gene expression signals on gene therapy vectors and the utility of EBV sequences in particular for increasing stable gene expression... - The therapeutic potential of phiC31 integrase as a gene therapy systemMarisa Karow
Stanford University School of Medicine, Department of Genetics, Stanford, CA 94305 5120, USA
Expert Opin Biol Ther 11:1287-96. 2011..The ?C31 integrase system displays an outstanding fit for use in pluripotent stem cells, and this area is expected to be the subject of intense development... - Gene transfer to rabbit retina with electron avalanche transfectionThomas W Chalberg
Departments of Genetics, Stanford University, Stanford, CA 94305, USA
Invest Ophthalmol Vis Sci 47:4083-90. 2006..CONCLUSIONS: Electron avalanche transfection is a powerful new technology for safe DNA delivery that has great promise as a nonviral system of gene transfer... - Site-specific genomic strategies for gene therapyJoylette L Portlock
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA
Curr Opin Mol Ther 5:376-82. 2003..We believe that these approaches hold promise for site-specific, safe and efficient gene therapy... - Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell linesYoshinori Ishikawa
Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan
J Gene Med 8:646-53. 2006..CONCLUSIONS: This study supports the possible future use of this phiC31 integrase-mediated genomic integration strategy as an alternative gene therapy approach for treating SCID-X1... - Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cellsOliver Dorigo
Molecular Biology Institute, University of California at Los Angeles, 611 Charles E. Young Dr. East, Los Angeles, CA 90095-1570, USA
J Virol 78:6556-66. 2004..This novel gene transfer system has the potential to confer long-term episomal transgene expression and therefore to correct genetic defects with reduced vector-related toxicity and without insertional mutagenesis... - Nucleofection of muscle-derived stem cells and myoblasts with phiC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblastsSimon P Quenneville
, Centre de Recherche du CHUL, CHUQ, , , Sainte-Foy, , Canada, G1V 4G2
Mol Ther 10:679-87. 2004..A nonviral approach combining nucleofection and the phiC31 integrase may eventually permit safe autotransplantation of genetically modified cells to patients... - Site-specific integration for high-level protein production in mammalian cellsBhaskar Thyagarajan
Poetic Genetics, LLC, Burlingame, CA, USA
Methods Mol Biol 308:99-106. 2005 - In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene deliveryPatrice K Held
Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Mol Ther 11:399-408. 2005..The stability of transgene expression, relatively high integration frequency, and significant site specificity that characterize the phiC31 integration system suggest that it may have utility in many gene therapy settings...
Research Grants
- Retinal Gene Therapy by Site-Specific IntegrationMICHELE CALOS; Fiscal Year: 2006..These experiments will move the phiC31 integrase ..
- Transferring integrase technology to animalsMICHELE CALOS; Fiscal Year: 2006..By scaling up to animals more similar to humans, these experiments will move the site-specific integrase technology closer to the clinic. ..
- Custom integration tools for functional genomicsMICHELE CALOS; Fiscal Year: 2003..We develop the appropriate DNA shuffling and genetic screening methods here for generation of a library of custom integration tools. The strategy is scalable to reach essentially all genes in the mouse genome. ..
- Transferring integrase technology to animalsMICHELE CALOS; Fiscal Year: 2003..Success in these experiments will lead to application of this innovative technology to other gene therapy settings and progress toward clinical trials in patients. ..
- SITE-SPECIFIC INTEGRATION FOR GENE THERAPYMICHELE CALOS; Fiscal Year: 2003..The novel site-specific integration strategy developed here can be used to add value to most current gene therapy vectors. ..