Research Topics
| STEVEN BOXERSummaryAffiliation: Stanford University Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Molecular transport and organization in supported lipid membranesS G Boxer
Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
Curr Opin Chem Biol 4:704-9. 2000..Controlled interactions between supported membranes and cells, particularly from the immune system, provide direct insight into cell-cell surface interactions...- Advances in imaging secondary ion mass spectrometry for biological samplesSteven G Boxer
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Annu Rev Biophys 38:53-74. 2009..Recent applications of imaging mass spectrometry to cell biology, microbial communities, and biosynthetic pathways are summarized briefly, and studies of biological membrane organization are described in greater depth... - Stark realitiesSteven G Boxer
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
J Phys Chem B 113:2972-83. 2009.... - Diffusive dynamics of vesicles tethered to a fluid supported bilayer by single-particle trackingChiaki Yoshina-Ishii
Department of Chemistry, Stanford University, Stanford, California 94305, USA
Langmuir 22:5682-9. 2006..This shows that a significant reduction in D can be observed while maintaining normal diffusion behavior on the time scale of our experiments... - Charge transfer in photoacids observed by stark spectroscopyLisa N Silverman
Department of Chemistry, Stanford University, Stanford, California 94305, USA
J Phys Chem A 112:10244-9. 2008..Conversely, for the cationic (ammonium) photoacid studied, photoexcitation of a particular electronic state results in much smaller charge transfer for the protonated state than for the deprotonated state... - DNA-tethered membranes formed by giant vesicle ruptureMinsub Chung
Department of Chemistry, Stanford University, CA 94305 5080, USA
J Struct Biol 168:190-9. 2009..In both cases, lipid mobility is high and there is a negligible immobile fraction. Thus, these architectures offer a flexible platform for the assembly of lipid bilayers at a well-defined distance from a solid support... - Micropattern formation in supported lipid membranesJay T Groves
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Acc Chem Res 35:149-57. 2002..Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes... - Kinetics of DNA-mediated docking reactions between vesicles tethered to supported lipid bilayersYee Hung M Chan
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 104:18913-8. 2007..These results provide the basis for the application of tethered vesicles to study other membrane reactions including protein-mediated docking and fusion... - Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 2. Unusual photophysical propertiesXinghua Shi
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
Biochemistry 46:12014-25. 2007..It is speculated that two different orientations of the Asp introduced at position 148, not distinguishable by chromatography, mass spectrometry, or X-ray crystallography, give rise to the two functionally distinct populations... - Charge delocalization in the special-pair radical cation of mutant reaction centers of Rhodobacter sphaeroides from Stark spectra and nonadiabatic spectral simulationsPakorn Kanchanawong
Biophysics Program and Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA
J Phys Chem B 110:18688-702. 2006.... - Green fluorescent protein variants as ratiometric dual emission pH sensors. 2. Excited-state dynamicsTim B McAnaney
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA
Biochemistry 41:15489-94. 2002..At low pH, excited-state proton transfer is slowed to the point where it is no longer rate limiting... - Proximal ligand motions in H93G myoglobinStefan Franzen
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
Eur J Biochem 269:4879-86. 2002..The results suggest that ligand conformational changes in response to dynamic motions of the globin on the nanosecond and longer time scales are a general feature of the H93G proximal cavity mutant... - Green fluorescent protein variants as ratiometric dual emission pH sensors. 3. Temperature dependence of proton transferTim B McAnaney
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Biochemistry 44:8701-11. 2005.... - Probing the structure of supported membranes and tethered oligonucleotides by fluorescence interference contrast microscopyCaroline M Ajo-Franklin
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Langmuir 21:4976-83. 2005.... - Protonation, photobleaching, and photoactivation of yellow fluorescent protein (YFP 10C): a unifying mechanismTim B McAnaney
Department of Chemistry, Stanford University, CA 94305, USA
Biochemistry 44:5510-24. 2005..Using a pulsed laser for photobleaching resulted in decarboxylation of YFP as indicated by the mass spectrum. These observations are accounted for in a unifying kinetic scheme... - Supported membrane composition analysis by secondary ion mass spectrometry with high lateral resolutionCarine Galli Marxer
Department of Chemistry, Stanford University, Stanford, California 94305, USA
Biophys J 88:2965-75. 2005..This approach has the potential to provide a composition-specific analysis of membrane organization that compliments other imaging modalities... - Vibrational Stark effects calibrate the sensitivity of vibrational probes for electric fields in proteinsIan T Suydam
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Biochemistry 42:12050-5. 2003..The measured Stark tuning rates, peak positions, and extinction coefficients provide the primary information needed to design amino acid analogues or labels to act as probes of local environments in proteins... - Controlling two-dimensional tethered vesicle motion using an electric field: interplay of electrophoresis and electro-osmosisChiaki Yoshina-Ishii
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Langmuir 22:2384-91. 2006..Electric fields are effective tools to direct tethered vesicles and concentrate them and to measure the tethered vesicle's electrostatic properties...
Research Grants
- Membrane Fusion and Dynamics Using Supported BilayersSTEVEN BOXER; Fiscal Year: 2007..abstract_text> ..
- Electrostatics and Dynamics in ProteinsSTEVEN BOXER; Fiscal Year: 2007..Experiments are proposed on GFPs that emit at multiple wavelengths (dual emission GFPs), on the mechanism of communication between the chromophore and bulk solvent, and on GFP chromophores containing non-natural amino acids. ..
- Membrane Fusion, Organization, and Dynamics Using Supported BilayersSTEVEN BOXER; Fiscal Year: 2009..This proposal outlines new methods for studying membranes and membrane-associated proteins that can impact our understanding of biological function and organization, as well as impact biotechnology. ..
- Membrane Fusion, Organization, and Dynamics Using Supported BilayersSteven G Boxer; Fiscal Year: 2010..This proposal outlines new methods for studying membranes and membrane-associated proteins that can impact our understanding of biological function and organization, as well as impact biotechnology. ..
- Membrane Fusion, Organization, and Dynamics Using Supported BilayersSTEVEN BOXER; Fiscal Year: 2009..Each aim depends upon the development of new supported lipid bilayer architectures and analytical methods that can have a broad impact on studies of biological membranes. Project Description Page 6 ..
- Electrostatics and Dynamics in ProteinsSteven G Boxer; Fiscal Year: 2010..New experiments are also proposed for GFP which is the most widely used fluorescent protein for cell-based imaging. ..
- Membrane Fusion, Organization, and Dynamics Using Supported BilayersSTEVEN BOXER; Fiscal Year: 2009..This proposal outlines new methods for studying membranes and membrane-associated proteins that can impact our understanding of biological function and organization, as well as impact biotechnology. ..
- ELECTROSTATICS AND DYNAMICS IN MYOGLOBIN MUTANTSSTEVEN BOXER; Fiscal Year: 1993..Advances in ultra-fast fluorescence methods, theories of liquid dynamics and the possibility to examine the role of individual amino acid residues by site-specific mutagenesis make this possible...
- BIOMOLECULAR STRUCTURE/DYNAMICS--NUCLEAR POLARIZATIONSTEVEN BOXER; Fiscal Year: 1980..This technique will be applied to measure internal motion of diamagnetic porphyrins and chlorophylls in apo-myoglobin (the chloroglobins). ..
- Electrostatics and Dynamics in ProteinsSTEVEN BOXER; Fiscal Year: 2009..New experiments are also proposed for GFP which is the most widely used fluorescent protein for cell-based imaging. ..