E L White

Summary

Affiliation: Southern Research Institute
Country: USA

Publications

  1. ncbi Slow polymerization of Mycobacterium tuberculosis FtsZ
    E L White
    Drug Discovery Division, Southern Research Institute, Birmingham, AL 35205, USA
    J Bacteriol 182:4028-34. 2000
  2. ncbi Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase
    E Lucile White
    Drug Discovery Division, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL 35205, USA
    FEMS Microbiol Lett 232:101-5. 2004
  3. ncbi A novel inhibitor of Mycobacterium tuberculosis pantothenate synthetase
    E Lucile White
    Southern Research Institute, Birmingham, AL 35205 5305, USA
    J Biomol Screen 12:100-5. 2007
  4. ncbi Antioxidant activity of michellamine alkaloids
    E L White
    Biochemistry Department, Southern Research Institute, Birmingham, AL 35205, USA
    Anticancer Res 19:1033-5. 1999
  5. ncbi Screening of potential cancer preventing chemicals as aromatase inhibitors in an in vitro assay
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    Anticancer Res 19:1017-20. 1999
  6. ncbi Michellamine alkaloids inhibit protein kinase C
    E L White
    Department of Biochemistry, Southern Research Institute, Birmingham, Alabama 35205, USA
    Arch Biochem Biophys 365:25-30. 1999
  7. ncbi Screening of potential cancer preventing chemicals as antioxidants in an in vitro assay
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    Anticancer Res 18:769-73. 1998
  8. ncbi Safety factors involved in the extraction of biologically active proteins from human immunodeficiency virus
    E L White
    Southern Research Institute, Birmingham, AL 35205, USA
    J Virol Methods 70:113-5. 1998
  9. ncbi Polyamine pools in HIV-infected cells
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    J Acquir Immune Defic Syndr Hum Retrovirol 17:101-3. 1998
  10. ncbi Optimization of the reverse transcriptase assay for the detection of viral burden in mice infected with Rauscher murine leukemia virus
    E L White
    Southern Research Institute, Birmingham, AL 35205, USA
    J Virol Methods 62:63-70. 1996

Research Grants

  1. Screen for Mycobacterium Tuberculosis (RMI)
    E White; Fiscal Year: 2005

Detail Information

Publications20

  1. ncbi Slow polymerization of Mycobacterium tuberculosis FtsZ
    E L White
    Drug Discovery Division, Southern Research Institute, Birmingham, AL 35205, USA
    J Bacteriol 182:4028-34. 2000
    ..tuberculosis FtsZ polymerization, inhibits GTP hydrolysis, and reduces the number and sizes of FtsZ polymers...
  2. ncbi Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase
    E Lucile White
    Drug Discovery Division, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL 35205, USA
    FEMS Microbiol Lett 232:101-5. 2004
    ..6 kDa. Gel filtration of the enzyme indicated that it was a monomer. Steady-state kinetic parameters were determined and the effect of pH and KCl on the enzyme examined. Methotrexate and trimethoprim inhibited the enzyme...
  3. ncbi A novel inhibitor of Mycobacterium tuberculosis pantothenate synthetase
    E Lucile White
    Southern Research Institute, Birmingham, AL 35205 5305, USA
    J Biomol Screen 12:100-5. 2007
    ..This coupled enzymatic reaction was used to screen 4080 compounds in a 96-well format. This discussion describes a novel inhibitor of PS that exhibits potential as an antimicrobial agent...
  4. ncbi Antioxidant activity of michellamine alkaloids
    E L White
    Biochemistry Department, Southern Research Institute, Birmingham, AL 35205, USA
    Anticancer Res 19:1033-5. 1999
    ..The alkaloids were more potent antioxidants in these assays than several compounds being considered clinically as chemoprevention agents...
  5. ncbi Screening of potential cancer preventing chemicals as aromatase inhibitors in an in vitro assay
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    Anticancer Res 19:1017-20. 1999
    ..Other compounds with known chemoprevention activity, such as curcumin and genistein, were inactive. This assay for aromatase inhibitors is a rapid, economical way of ranking compounds for further development as chemoprevention agents...
  6. ncbi Michellamine alkaloids inhibit protein kinase C
    E L White
    Department of Biochemistry, Southern Research Institute, Birmingham, Alabama 35205, USA
    Arch Biochem Biophys 365:25-30. 1999
    ..Molecular modeling showed that all three michellamines can bind in the active site cleft of the PKC kinase domain, to block both the ATP and the peptide substrate subsites...
  7. ncbi Screening of potential cancer preventing chemicals as antioxidants in an in vitro assay
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    Anticancer Res 18:769-73. 1998
    ..This fluorescence-based assay for antioxidant activity is a rapid, economical way of ranking antioxidants for further development in the National Cancer Institute's chemoprevention program...
  8. ncbi Safety factors involved in the extraction of biologically active proteins from human immunodeficiency virus
    E L White
    Southern Research Institute, Birmingham, AL 35205, USA
    J Virol Methods 70:113-5. 1998
    ..Conditions for the production of a noninfectious sample from a viral pellet that can be used to measure reverse transcriptase activity were determined...
  9. ncbi Polyamine pools in HIV-infected cells
    E L White
    Southern Research Institute, Birmingham, Alabama 35205, USA
    J Acquir Immune Defic Syndr Hum Retrovirol 17:101-3. 1998
    ..Our results indicate that inhibitors of this pathway will not be therapeutically useful in the treatment of AIDS...
  10. ncbi Optimization of the reverse transcriptase assay for the detection of viral burden in mice infected with Rauscher murine leukemia virus
    E L White
    Southern Research Institute, Birmingham, AL 35205, USA
    J Virol Methods 62:63-70. 1996
    ..The optimized assay method was effective in evaluating the antiviral activity of AZT in the Rauscher murine leukemia virus in vivo model. The assay is also amenable to automation if large numbers of assays are required...
  11. ncbi 2-Alkoxycarbonylaminopyridines: inhibitors of Mycobacterium tuberculosis FtsZ
    E Lucile White
    Drug Discovery Division, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL 35205, USA
    J Antimicrob Chemother 50:111-4. 2002
    ..tuberculosis in mouse bone marrow macrophages. Our results suggest that these types of compound might be developed into antitubercular drugs effective against the current multidrug-resistant strains of M. tuberculosis...
  12. ncbi PMTI, a broadly active unusual single-stranded polyribonucleotide, inhibits human immunodeficiency virus replication by multiple mechanisms
    R W Buckheit
    Microbiology Research Department, Southern Research Institute, Frederick, MD 21701, USA
    Antivir Chem Chemother 10:23-32. 1999
    ..These results suggest that the homopolyribonucleotide PMTI blocks HIV replication in human cells at its earliest stages by multiple mechanisms, inhibition of virus entry and inhibition of RT...
  13. ncbi A new 2-carbamoyl pteridine that inhibits mycobacterial FtsZ
    R C Reynolds
    Drug Discovery Division, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL 35205, USA
    Bioorg Med Chem Lett 14:3161-4. 2004
    ..The preparation of a new 2-carbamoyl pteridine, its activity data against FtsZ from M. tuberculosis (Mtb), and in vitro antibacterial data against Mtb strain H37Ra are presented...
  14. ncbi Crystallization of the Mycobacterium tuberculosis cell-division protein FtsZ
    A K Leung
    Drug Discovery Division, Southern Research Institute, Birmingham, AL 35205, USA
    Acta Crystallogr D Biol Crystallogr 56:1634-7. 2000
    ..Exceptional form II crystals diffract to at least 4.5 A resolution. Determination of the MtbFtsZ structure may advance the design of improved inhibitors of FtsZ polymerization...
  15. ncbi Significant anti-HIV activity of new modified polyanionic polymers in vitro
    R W Buckheit
    Southern Research Institute, Birmingham, Alabama
    Immunopharmacol Immunotoxicol 14:707-21. 1992
    ..Both compounds caused a significant increase in cellular proliferation as determined by the concentration-dependent increase in incorporation of radioactive precursors into cellular macromolecules...
  16. ncbi Unique anti-human immunodeficiency virus activities of the nonnucleoside reverse transcriptase inhibitors calanolide A, costatolide, and dihydrocostatolide
    R W Buckheit
    Infectious Disease Research Department, Serquest Southern Research Institute, Frederick, Maryland 21701, USA
    Antimicrob Agents Chemother 43:1827-34. 1999
    ....
  17. ncbi SJ-3366, a unique and highly potent nonnucleoside reverse transcriptase inhibitor of human immunodeficiency virus type 1 (HIV-1) that also inhibits HIV-2
    R W Buckheit
    Infectious Disease Research Department, Southern Research Institute, Frederick, Maryland 21701, USA
    Antimicrob Agents Chemother 45:393-400. 2001
    ..On the basis of its therapeutic index and multiple mechanisms of anti-HIV action, SJ-3366 represents an exciting new compound for use in HIV-infected individuals...
  18. ncbi Polymerization of C-terminally truncated Mycobacterium tuberculosis FtsZ is unlikely to be physiologically relevant
    David W Borhani
    Abbott Bioresearch Center, 100 Research Drive, Worcester, MA 01605, USA
    Microbiology 150:3903-6. 2004
  19. ncbi A cell-based luminescence assay is effective for high-throughput screening of potential influenza antivirals
    James W Noah
    Southern Research Institute, Drug Discovery Division, 2000 Ninth Avenue South, Birmingham, AL 35205, USA
    Antiviral Res 73:50-9. 2007
    ..This assay is translatable for screening against other influenza strains, such as avian flu, and may facilitate identification of antivirals for other viruses that induce CPE, such as West Nile or Dengue...
  20. ncbi Development and validation of a high-throughput screen for inhibitors of SARS CoV and its application in screening of a 100,000-compound library
    William E Severson
    Department of Biochemistry and Molecular Biology, Southern Research Institute, Birmingham, AL 35205, USA
    J Biomol Screen 12:33-40. 2007
    ..At a 10-microM concentration, 3 compounds with selective indexes (SI50) of > 53 were discovered...

Research Grants1

  1. Screen for Mycobacterium Tuberculosis (RMI)
    E White; Fiscal Year: 2005
    ..tuberculosis. 3) To study the effect of these novel inhibitors on the pantothenate pathway in M. smegmatis. 4) To co-crystallize these compounds with pantothenate synthetase to study their mode of binding ..