Research Topics
| P E DuffySummaryAffiliation: Seattle Biomedical Research Institute Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Two DBLgamma subtypes are commonly expressed by placental isolates of Plasmodium falciparumMichal Fried
Seattle Biomedical Research Institute, 4 Nickerson Street, WA 98109, USA
Mol Biochem Parasitol 122:201-10. 2002..The findings are consistent with earlier serologic evidence that surface antigens of placental parasites have conserved features, and suggest that vaccines based on DBLgamma may only need to target a limited number of variants...- Artemisinin combination therapiesPatrick E Duffy
Malaria Program, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Lancet 367:2037-9. 2006 - Maternal peripheral blood level of IL-10 as a marker for inflammatory placental malariaEdward R Kabyemela
Mother Offspring Malaria Studies MOMS Project, Seattle Biomedical Research Institute, Seattle WA 98109, USA
Malar J 7:26. 2008..Diagnosis is difficult because PM is often asymptomatic, peripheral blood smear examination detects parasitemia as few as half of PM cases, and no peripheral markers have been validated for placental inflammation... - Malaria sporozoite proteome leaves a trailMarissa Vignali
Malaria Program, Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
Genome Biol 10:216. 2009..The malaria parasite sporozoite proteome changes during maturation, revealing proteins specifically expressed in the stage that infects the human host... - Plasmodium in the placenta: parasites, parity, protection, prevention and possibly preeclampsiaPatrick E Duffy
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Seattle, WA 98109, USA
Parasitology 134:1877-81. 2007..The burden of disease due to pregnancy malaria, and the benefits of an effective vaccine, may be much greater than is currently appreciated... - Malaria in the pregnant womanP E Duffy
Seattle Biomedical Research Institute, 307 Westlake Avenue, Seattle, WA, USA
Curr Top Microbiol Immunol 295:169-200. 2005..A vaccine that prevents pregnancy malaria due to P. falciparum could be delivered before first pregnancy, and would have an enormous impact on mother-child health in tropical areas... - Rolling back a malaria epidemic in South AfricaPatrick E Duffy
Seattle Biomedical Research Institute, Seattle, Washington, United States of America
PLoS Med 2:e368. 2005 - Plasmodium falciparum adhesion in the placentaPatrick E Duffy
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute, 4 Nickerson ST STE 200, Seattle, WA 98109 1651, USA
Curr Opin Microbiol 6:371-6. 2003..Adhesins and other surface proteins of placental parasites are targets of naturally occurring antibodies associated with protection and therefore could be used to develop vaccines that prevent malaria in pregnancy... - Malaria vaccines: using models of immunity and functional genomics tools to accelerate the development of vaccines against Plasmodium falciparumPatrick E Duffy
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
Vaccine 23:2235-42. 2005..These candidate antigens are then subjected to detailed studies according to criteria established by the understanding of pathogenesis and protective immunity, to identify the optimal antigens for inclusion in subunit vaccines... - Drug combinations for malaria: time to ACT?Patrick E Duffy
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute, WA 98109, Seattle, USA
Lancet 363:3-4. 2004 - Antibodies that inhibit Plasmodium falciparum adhesion to chondroitin sulfate A are associated with increased birth weight and the gestational age of newbornsPatrick E Duffy
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
Infect Immun 71:6620-3. 2003..019) and 2 weeks more mature (P = 0.002) than babies delivered to secundigravidas without antiadhesion activity. Our findings support the development of antiadhesion vaccines to prevent poor fetal outcomes due to pregnancy malaria... - VAR2CSA domains expressed in Escherichia coli induce cross-reactive antibodies to native proteinAndrew V Oleinikov
Seattle Biomedical Research Institute, University of Washington, Seattle, USA
J Infect Dis 197:1119-23. 2008..This is the first report that E. coli-expressed VAR2CSA domains induce antibody to native VAR2CSA... - Competitive facilitation of drug-resistant Plasmodium falciparum malaria parasites in pregnant women who receive preventive treatmentW E Harrington
Seattle Biomedical Research Institute, 307 Westlake Avenue N, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 106:9027-32. 2009..Use of partially effective anti-malarial agents for IPTp may exacerbate malaria infections in the setting of widespread drug resistance... - Six genes are preferentially transcribed by the circulating and sequestered forms of Plasmodium falciparum parasites that infect pregnant womenSusan E Francis
Seattle Biomedical Research Institute, 307 Westlake Ave N, Seattle, WA 98109 5219, USA
Infect Immun 75:4838-50. 2007..These findings suggest that a suite of genes may be important for the genesis of the placental binding phenotype of P. falciparum and may provide novel targets for therapeutic intervention... - Analysis of CSA-binding parasites and antiadhesion antibodiesMichal Fried
Seattle Biomedical Research Institute, Seattle, WA, USA
Methods Mol Med 72:555-60. 2002 - Immunization with VAR2CSA-DBL5 recombinant protein elicits broadly cross-reactive antibodies to placental Plasmodium falciparum-infected erythrocytesMarion Avril
Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA 98109 5219, USA
Infect Immun 78:2248-56. 2010..These findings demonstrate that the major PfEMP1 variant expressed by placental isolates exposes strain-transcendent epitopes that can be targeted by vaccination and may have application for pregnancy malaria vaccine development... - PhD: a web database application for phenotype data managementJ L Li
Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People s Republic of China
Bioinformatics 21:3443-4. 2005..By applying the EAV model, this application allows users to manage arbitrary phenotypes and customize data entry forms; therefore, it is suitable for different and multi-center projects... - The distinct proteome of placental malaria parasitesMichal Fried
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Mol Biochem Parasitol 155:57-65. 2007..One of these hypothetical proteins, PFI1785w, is a 42kDa molecule detected by Western blot in parasites infecting pregnant women but not those infecting children... - Effects of sex, parity, and sequence variation on seroreactivity to candidate pregnancy malaria vaccine antigensAndrew V Oleinikov
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
J Infect Dis 196:155-64. 2007..Two IE surface molecules, VAR1CSA and VAR2CSA, bind CSA in vitro and are potential vaccine candidates... - High throughput functional assays of the variant antigen PfEMP1 reveal a single domain in the 3D7 Plasmodium falciparum genome that binds ICAM1 with high affinity and is targeted by naturally acquired neutralizing antibodiesAndrew V Oleinikov
Seattle Biomedical Research Institute, Seattle, Washington, USA
PLoS Pathog 5:e1000386. 2009..Our high throughput approach will significantly accelerate studies of PfEMP1 binding domains and protective antibody responses... - A novel histological grading scheme for placental malaria applied in areas of high and low malaria transmissionAtis Muehlenbachs
Seattle Biomedical Research Institute, University of Washington, Seattle, USA
J Infect Dis 202:1608-16. 2010..Histologic measurements during placental malaria might provide surrogate end points for interventional trials, but existing histologic schemes capture limited complexity and are not consistently used among study sites... - Evidence for globally shared, cross-reacting polymorphic epitopes in the pregnancy-associated malaria vaccine candidate VAR2CSAMarion Avril
Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA 98109 5219, USA
Infect Immun 76:1791-800. 2008..This study demonstrates that VAR2CSA contains common polymorphic epitopes that are shared between geographically diverse CSA-binding lines... - Plasmodium falciparum: chondroitin sulfate A is the major receptor for adhesion of parasitized erythrocytes in the placentaMichal Fried
Seattle Biomedical Research Institute, Seattle, WA, USA
Exp Parasitol 113:36-42. 2006..P. falciparum adhesion in the placenta appears to be a non-redundant process that requires CSA as a receptor. Vaccines that elicit functional antibodies against CSA-binding parasites may confer resistance to pregnancy malaria... - Maternal immunization and malaria in pregnancyPatrick E Duffy
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute, 4 Nickerson Street, Suite 200, Seattle, WA 98119, USA
Vaccine 21:3358-61. 2003.... - Mass spectrometric analysis of Plasmodium falciparum erythrocyte membrane protein-1 variants expressed by placental malaria parasitesMichal Fried
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
Proteomics 4:1086-93. 2004..These novel PfEMP1 variants require further study to confirm whether they play a role in placental malaria... - Are we losing artemisinin combination therapy already?Patrick E Duffy
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute and Walter Reed Army Institute of Research, Seattle, WA 98109, USA
Lancet 366:1908-9. 2005 - Red blood cells that do and red blood cells that don't: how to resist a persistent parasitePatrick E Duffy
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
Trends Parasitol 22:99-101. 2006..HbC-containing red blood cells have modified displays of malaria surface proteins that reduce parasite adhesiveness and could reduce the risk of severe disease... - Antigens for pre-erythrocytic malaria vaccines: building on successC Speake
Malaria Program, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Parasite Immunol 31:539-46. 2009.... - Decreased susceptibility to Plasmodium falciparum infection in pregnant women with iron deficiencyEdward R Kabyemela
Mother Offspring Malaria Studies Project, Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
J Infect Dis 198:163-6. 2008..Interventional trials of iron and folate supplementation during pregnancy in malaria-endemic regions in Africa are urgently needed to ascertain the benefits and risks of this intervention... - Hypertension and maternal-fetal conflict during placental malariaAtis Muehlenbachs
MOMS Project, Seattle Biomedical Research Institute, Seattle, Washington, United States of America
PLoS Med 3:e446. 2006..Because placental inflammation causes poor fetal outcomes, we hypothesize that fetal mechanisms that promote sVEGFR1 expression may be under selective pressure during first pregnancies in malaria-endemic areas... - Maternal malaria and gravidity interact to modify infant susceptibility to malariaTheonest K Mutabingwa
Malaria Antigen Discovery Program, Seattle Biomedical Research Institute, Seattle, Washington, USA
PLoS Med 2:e407. 2005.... - Natural selection of FLT1 alleles and their association with malaria resistance in uteroAtis Muehlenbachs
Mother Offspring Malaria Studies Project, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 105:14488-91. 2008..This indicates that FLT1 is under natural selection in a malaria endemic area and that human exposure to malaria can influence the evolutionary genetics of the maternal-fetal relationship... - Malaria is related to decreased nutritional status among male adolescents and adults in the setting of intense perennial transmissionJennifer F Friedman
United States Department of State, Institute of International Education, Fulbright Fellowships, Washington, DC, USA
J Infect Dis 188:449-57. 2003..These effects of malaria may have pervasive health and socioeconomic consequences in areas where malaria is endemic... - Fetal responses during placental malaria modify the risk of low birth weightEdward R Kabyemela
MOMS Project, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Infect Immun 76:1527-34. 2008..Because fetal cells are a source of placental IFN-gamma and cord ferritin, the fetal response to PM may modify the risk of LBW... - Plasma levels of apolipoprotein A1 in malaria-exposed primigravidae are associated with severe anemiaDavid C Simpson
Seattle Biomedical Research Institute, Seattle, Washington, United States of America
PLoS ONE 5:e8822. 2010..Here we aimed to identify additional changes in the plasma proteome associated with pregnancy malaria that may contribute to the development of malaria-related maternal anemia... - An unusual presentation of placental malaria: a single persisting nidus of sequestered parasitesAtis Muehlenbachs
Mother Offspring Malaria Studies Project, Seattle Biomedical Research Institute, Seattle WA 98109, USA
Hum Pathol 38:520-3. 2007..This finding suggests ring stage-infected erythrocytes sequester in vivo, or P falciparum can persist as a dormant blood stage form... - Genome-wide expression analysis of placental malaria reveals features of lymphoid neogenesis during chronic infectionAtis Muehlenbachs
Mother Offspring Malaria Study MOMS Project, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Seattle, WA 98109, USA
J Immunol 179:557-65. 2007..We propose a model where the production of Ig during chronic malaria may enhance inflammation by attracting and activating macrophages that, in turn, recruit B cells to further produce Ig in the intervillous spaces... - Protective human immunity as a vaccine discovery tool for falciparum malariaChristian P Nixon
International Health Institute, Department of Pediatrics, Brown University, Providence, Rhode Island 02912, USA
Transfusion 45:81S-87S. 2005..falciparum infection. Our results demonstrate that naturally acquired protective antibody responses are useful tools to identify vaccine candidates for falciparum malaria... - Malaria: progress, perils, and prospects for eradicationBrian M Greenwood
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
J Clin Invest 118:1266-76. 2008..Insights into parasite biology, human immunity, and vector behavior will guide efforts to translate parasite and mosquito genome sequences into novel interventions... - Antibodies to rhoptry-associated membrane antigen predict resistance to Plasmodium falciparumChristian P Nixon
International Health Institute, Brown University, Providence, Rhode Island 02912, USA
J Infect Dis 192:861-9. 2005..In summary, RAMA-pr is a rationally identified vaccine candidate that is preferentially recognized by antibodies produced by humans with a high level of naturally acquired resistance to P. falciparum infection... - Malaria in pregnancy: pathogenesis and immunityStephen J Rogerson
Department of Medicine, University of Melbourne, Parkville, Victoria, Australia
Lancet Infect Dis 7:105-17. 2007.... - Phase 2a trial of 0, 1, and 3 month and 0, 7, and 28 day immunization schedules of malaria vaccine RTS,S/AS02 in malaria-naïve adults at the Walter Reed Army Institute of ResearchKent E Kester
Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
Vaccine 26:2191-202. 2008.... - New interventions for malaria: mining the human and parasite genomesFrancine Ntoumi
Research Unit, Hopital Albert Schweitzer, Lambarene, Gabon
Am J Trop Med Hyg 77:270-5. 2007..However, genomics studies of human populations raise important ethical issues, such as the disposition of data related to disease susceptibility or paternity, and the ability of communities to understand the nature of the research... - Profound bias in interferon-gamma and interleukin-6 allele frequencies in western Kenya, where severe malarial anemia is common in childrenIan S Gourley
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA
J Infect Dis 186:1007-12. 2002..IFN-gamma and IL-6 genotypes may play roles in the development of severe malaria and could contribute to the relative frequency of severe malarial anemia or cerebral malaria in exposed populations...
Research Grants
- MATERNAL MALARIA--PARASITE ADHESION AND ANTIBODIESPatrick Duffy; Fiscal Year: 2001..Further, the delineation of binding domains and B cell epitopes will provide the basis to design an anti-adhesion vaccine to protect primigravid and other women susceptible to placental infection with the CSA-binding parasites. ..
- Pathogenesis of falciparum malaria in infancyPatrick Duffy; Fiscal Year: 2004..Identifying new therapies for children is an urgent public health goal, but is not a funding priority for the United States Department of Defense. ..
- BIOSTATISTICS AND DATA MANAGEMENT COREPatrick Duffy; Fiscal Year: 2005..The Data Core in Morogoro will be fully autonomous, with a Data Manager and data entry team under the supervision of Dr. Mutabingwa. ..