Research Topics
| Blake A SimmonsSummaryAffiliation: Sandia National Laboratories Country: USA Publications
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Detail Information
Publications
Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharificationChenlin Li
Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA, USA
Bioresour Technol 101:4900-6. 2010..These results indicate that ionic liquid pretreatment may offer unique advantages when compared to the dilute acid pretreatment process for switchgrass. However, the cost of the ionic liquid process must also be taken into consideration...- Advances in modifying lignin for enhanced biofuel productionBlake A Simmons
DOE Joint BioEnergy Institute, Emeryville, CA 94608, USA
Curr Opin Plant Biol 13:313-20. 2010..The realization of advanced analytical techniques and an increasing number of plant genomes are enabling researchers to take a systems approach towards understanding and engineering lignin to develop these optimal feedstocks... - Next-generation biomass feedstocks for biofuel productionBlake A Simmons
Joint BioEnergy Institute, Emervyille, CA 94608, USA
Genome Biol 9:242. 2008..Here we review the outlook and challenges for meeting hoped-for production targets for such biofuels in the United States... - Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stoverChenlin Li
Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA, USA
Bioresour Technol 102:6928-36. 2011..These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each... - Glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrassJohn M Gladden
Joint BioEnergy Institute, Emeryville, CA 94608, USA
Appl Environ Microbiol 77:5804-12. 2011.... - High-throughput enzymatic hydrolysis of lignocellulosic biomass via in-situ regenerationRajiv Bharadwaj
Technology Division, Joint BioEnergy Institute, Emeryville, CA, United States
Bioresour Technol 102:1329-37. 2011..The proposed method can be used to screen individual cellulases as well as to develop novel cellulase cocktails... - Bioenergy feedstock-specific enrichment of microbial populations during high-solids thermophilic deconstructionAmitha P Reddy
Joint BioEnergy Institute, Emeryville, California, USA
Biotechnol Bioeng 108:2088-98. 2011..The results demonstrate a successful approach for enrichment of unique microbial communities and enzymes active in a thermophilic high-solids environment... - Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticusJoshua I Park
Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA 94608, USA
Bioresour Technol 102:5988-94. 2011.... - Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coliGregory Bokinsky
Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608, USA
Proc Natl Acad Sci U S A 108:19949-54. 2011..With improvements in both biofuel synthesis pathways and biomass digestion capabilities, our approach could provide an economical route to production of advanced biofuels... - Molecular dynamics study of polysaccharides in binary solvent mixtures of an ionic liquid and waterHanbin Liu
Deconstruction Division, Joint BioEnergy Institute, Emeryville, California, USA
J Phys Chem B 115:10251-8. 2011..The analysis of this intermediate state provides new insight into the molecular driving forces present in this ternary system... - Molecular simulations provide new insights into the role of the accessory immunoglobulin-like domain of Cel9AHanbin Liu
Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA, United States
FEBS Lett 584:3431-5. 2010..Free energy perturbation simulations indicate that the Ig-like domain stabilizes the catalytic domain and may be responsible for the enhanced thermostability of Cel9A... - Transition of cellulose crystalline structure and surface morphology of biomass as a function of ionic liquid pretreatment and its relation to enzymatic hydrolysisGang Cheng
Joint BioEnergy Institute, Emeryville, California, USA
Biomacromolecules 12:933-41. 2011..By comparison, the result for the biomass samples suggests that another factor, likely lignin-carbohydrate complexes, also impacts the rate of cellulose hydrolysis in addition to cellulose crystallinity... - Neutron reflectometry and QCM-D study of the interaction of cellulases with films of amorphous celluloseGang Cheng
Joint BioEnergy Institute, Emeryville, CA, USA
Biomacromolecules 12:2216-24. 2011..In contrast, over the same time period, the endoglucanase was active mainly at the surface of the film and did not increase the surface roughness... - Understanding tissue specific compositions of bioenergy feedstocks through hyperspectral Raman imagingLan Sun
Joint BioEnergy Institute, Emeryville, California 94608, USA
Biotechnol Bioeng 108:286-95. 2011..The Raman mapping methods developed on corn stover were also validated on E. globulus and clearly highlighted their difference in lignin composition... - Rapid determination of syringyl: guaiacyl ratios using FT-Raman spectroscopyLan Sun
Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, 5885 Hollis Street, Emeryville, California 94608, USA
Biotechnol Bioeng 109:647-56. 2012..This technique holds significant promise in the rapid screening of engineered feedstocks as part of a comprehensive screening methodology that is correlated with biomass recalcitrance... - DNA-mediated delivery of lipophilic molecules via hybridization to DNA-based vesicular aggregatesPaul M Dentinger
Sandia National Laboratories, P O Box 969, Livermore, California 94550, USA
Langmuir 22:2935-7. 2006..Upon exposure to the complementary strand, the pyrene dye is released from the structures, showing that the scheme can be used for unlabeled or DNA-mediated drug delivery... - Microfluidic glycosyl hydrolase screening for biomass-to-biofuel conversionRajiv Bharadwaj
Technology Division, The Joint Bioenergy Institute, Emeryville, California 94608, United States
Anal Chem 82:9513-20. 2010..The proposed microscale approach is ideal for quantitative large-scale screening of enzyme libraries for biomass hydrolysis, for development of energy feedstocks, and for polysaccharide sequencing... - Substrate perturbation alters the glycoside hydrolase activities and community composition of switchgrass-adapted bacterial consortiaJohn M Gladden
Joint BioEnergy Institute, Emeryville, California 94608, USA
Biotechnol Bioeng 109:1140-5. 2012..These results demonstrate that the composition of biomass substrates influences the glycoside hydrolase activities and community composition of biomass-deconstructing bacterial consortia... - Understanding the interactions of cellulose with ionic liquids: a molecular dynamics studyHanbin Liu
Deconstruction Division, Joint BioEnergy Institute, Emeryville, California 94608, USA
J Phys Chem B 114:4293-301. 2010..To our knowledge, this report is the first theoretical study that addresses the key factors in cellulose dissolution using an ionic liquid... - Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost communityMartin Allgaier
Deconstruction Division, Joint BioEnergy Institute, Emeryville, California, United States of America
PLoS ONE 5:e8812. 2010.... - Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrassSeema Singh
Joint BioEnergy Institute, Deconstruction Division, Emeryville, California, USA
Biotechnol Bioeng 104:68-75. 2009..In comparison to untreated biomass, ionic liquid pretreated biomass produces cellulose that is efficiently hydrolyzed with commercial cellulase cocktail with high sugar yields over a relatively short time interval... - Addressing the need for alternative transportation fuels: the Joint BioEnergy InstituteHarvey W Blanch
Joint BioEnergy Institute, Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
ACS Chem Biol 3:17-20. 2008 - Co-production of ethanol, biogas, protein fodder and natural fertilizer in organic farming--evaluation of a concept for a farm-scale biorefineryPiotr Oleskowicz-Popiel
Joint BioEnergy Institute, 5885 Hollis St, Emeryville, CA 94608, USA
Bioresour Technol 104:440-6. 2012..Setting a methane selling price according to available incentives for "green electricity" (0.72 USD/m(3)) led to a minimum ethanol selling price of 1.89 USD/L (project lifetime 25 yr, at a discount rate 10%)... - Biomass deconstruction to sugarsHarvey W Blanch
Joint BioEnergy Institute, Emeryville, CA 94608, USA
Biotechnol J 6:1086-102. 2011..As pretreatment represents one of the major costs in converting biomass to fuels, the factors that contribute to pretreatments costs, and their impact on overall process economics, are described...