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| D James MorreSummaryAffiliation: Purdue University Country: USA Publications
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Publications
Regular oscillatory behavior of aqueous solutions of CuII salts related to effects on equilibrium dynamics of ortho/para hydrogen spin isomers of waterD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S University Street, West Lafayette, IN 47907 2064, United States
J Inorg Biochem 102:260-7. 2008..Thus, the time keeping oscillations of ECTO-NOX proteins appear to reflect the equilibrium dynamics of ortho-para hydrogen atom spin ratios of water where the presence of metal cations such as Cu(II) in solution determine period length...
Preferential inhibition by (-)-epigallocatechin-3-gallate of the cell surface NADH oxidase and growth of transformed cells in cultureD J Morre
Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biochem Pharmacol 60:937-46. 2000..The findings correlate inhibition of cell surface NADH oxidase activity and inhibition of growth with EGCg-induced apoptosis...
Biochemical basis for the biological clockD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, 136 Hansen Life Sciences Research Building, Purdue University, West Lafayette, IN 47907, USA
Biochemistry 41:11941-5. 2002....
ATP-dependent and drug-inhibited vesicle enlargement reconstituted using synthetic lipids and recombinant proteinsD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, HANS Life Sciences Research Building, Purdue University, 201 South University Street, West Lafayette, IN 47907 2064, USA
Biofactors 28:105-17. 2006..Binding of ATP and subsequent hydrolysis and release of ADP would advance the ATPase hexamer ratchet thereby both thinning the membrane and increasing the vesicle surface...
Phenoxodiol treatment alters the subsequent response of ENOX2 (tNOX) and growth of hela cells to paclitaxel and cisplatinD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907 2064, USA
Mol Biotechnol 42:100-9. 2009..In keeping with a speculative prion model, it seems as though the ENOX2 "remembers" the phenoxodiol and "teaches" other ENOX2 molecules to respond to paclitaxel and cisplatin as if phenoxodiol were still present...
arNOX activity of saliva as a non-invasive measure of coenzyme Q10 response in human trialsD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biofactors 32:231-5. 2008..The findings suggest that salivary arNOX provides a convenient and non-invasive method to monitor arNOX levels in clinical coenzyme Q10 intervention trials with the response levels paralleling those seen with serum and cellular arNOX...
Surface NADH oxidase of HeLa cells lacks intrinsic membrane binding motifsD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
Arch Biochem Biophys 392:251-6. 2001..Release was unaffected by protease inhibitors or divalent ions and was not accelerated by addition of cathepsin D. The findings suggest dissociable receptor binding as a possible basis for their plasma membrane association...
Entrainment in solution of an oscillating NADH oxidase activity from the bovine milk fat globule membrane with a temperature-compensated period length suggestive of an ultradian time-keeping (clock) functionD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette IN 47907, USA
Biochim Biophys Acta 1559:10-20. 2002..The cell surface NOX protein is the first reported example of an entrainable biochemical entity with a temperature-compensated periodicity potentially capable of functioning as an ultradian or circadian clock driver...
Auxin-activated NADH oxidase activity of soybean plasma membranes is distinct from the constitutive plasma membrane NADH oxidase and exhibits prion-like propertiesD James Morre
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
In Vitro Cell Dev Biol Plant 39:368-76. 2003..However, after several hours a dominant 24-min period emerges at the expense of the constitutive activity. A recruitment process analogous to that exhibited by prions is postulated to explain this behavior...
Cell enlargement of plant tissue explants oscillates with a temperature-compensated period of ca. 24 minD James Morre
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907 1333, USA
In Vitro Cell Dev Biol Plant 38:18-28. 2002....
Soybean cell enlargement oscillates with a temperature-compensated period length of ca. 24 minD J Morre
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907 1333, USA
In Vitro Cell Dev Biol Plant 37:19-23. 2001....
The plasma membrane-associated NADH oxidase of spinach leaves responds to blue lightD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Int J Plant Sci 163:543-7. 2002..The net effects of blue and red light were ultimately the same with a new maximum in the rate of NOX activity at 12+24=36 min (and every 24 min thereafter), but the mechanisms appear to be distinct...
Applications of aqueous two-phase partition to isolation of membranes from plants: a periodic NADH oxidase activity as a marker for right side-out plasma membrane vesiclesD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907 1333, USA
J Chromatogr B Biomed Sci Appl 743:369-76. 2000..g., ATPases, at the cytosolic surface. This report describes a periodic NADH oxidase as an enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions of vesicles for measurement of activity...
tNOX, an alternative target to COX-2 to explain the anticancer activities of non-steroidal anti-inflammatory drugs (NSAIDS)D James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Mol Cell Biochem 283:159-67. 2006..The findings provide evidence for a new drug target to account for anticancer effects of NSAIDS that occur independent of COX-2...
ECTO-NOX target for the anticancer isoflavene phenoxodiolD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Oncol Res 16:299-312. 2007..Activities of constitutive ECTO-NOX (CNOX) forms of either cancer or noncancer cells were unaffected by phenoxodiol to help explain how the cytotoxic effects of phenoxodiol may be restricted to cancer cells...
Preferential inhibition of the plasma membrane NADH oxidase (NOX) activity by diphenyleneiodonium chloride with NADPH as donorD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907 1333, USA
Antioxid Redox Signal 4:207-12. 2002....
Aging-related cell surface ECTO-NOX protein, arNOX, a preventive target to reduce atherogenic risk in the elderlyD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Rejuvenation Res 9:231-6. 2006..As such, the findings provide a rational basis for the antiaging activity of circulating coenzyme Q10 in the prevention of atherosclerosis and other aging-related oxidative changes in cell membranes and circulating lipoproteins...
Synergistic Capsicum-tea mixtures with anticancer activityD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 201 S University Avenue, West Lafayette, IN 47907 2064, USA
J Pharm Pharmacol 55:987-94. 2003..The activity of the protein target was inhibited by the tea catechins and the Capsicum vanilloids. As with growth, the tea and Capsicum preparations evaluated were synergistic in their inhibition of the target enzymatic activity...
Structural observations of time dependent oscillatory behavior of CuIICl2 solutions measured via extended X-ray absorption fine structureD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S University Street, West Lafayette, IN 47907 2064, USA
J Inorg Biochem 101:715-26. 2007..The temperature independence of the biological clock can thus be understood as the consequence of a physical rather than a chemical basis for the timing events...
Response of the regulatory oscillatory behavior of copperII-containing ECTO-NOX proteins and of CuIICl2 in solution to electromagnetic fieldsD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S University Street, West Lafayette, IN 47907 2064, United States
J Inorg Biochem 102:1812-8. 2008..Here, we provide results from Cu(II)Cl(2) solutions that demonstrate that ECTO-NOX-/Cu(II)-catalyzed oscillations in NADH oxidation are phased by exposure to low frequency electromagnetic fields...
Tea catechin synergies in inhibition of cancer cell proliferation and of a cancer specific cell surface oxidase (ECTO-NOX)D James Morre
Departments of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Pharmacol Toxicol 92:234-41. 2003....
tNOX is both necessary and sufficient as a cellular target for the anticancer actions of capsaicin and the green tea catechin (-)-epigallocatechin-3-gallatePin Ju Chueh
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biofactors 20:235-49. 2004..The findings show cell surface expression of tNOX as both necessary and sufficient for the cellular anticancer activities attributed to both EGCg and capsaicin...
Reciprocal relationship between cytosolic NADH and ENOX2 inhibition triggers sphingolipid-induced apoptosis in HeLa cellsThomas De Luca
Department of Foods and Nutrition, Purdue University, Stone Hall, 700 W State Street, West Lafayette, Indiana 47907 2059, USA
J Cell Biochem 110:1504-11. 2010..Treatments that stimulate cytosolic NADH production potentiate the antiproliferative effects of ENOX2 inhibitors while those that attenuate NADH production or stimulate plasma membrane electron transport confer a survival advantage...
Metabolite modulation of HeLa cell response to ENOX2 inhibitors EGCG and phenoxodiolLian Ying Wu
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Biochim Biophys Acta 1810:784-9. 2011....
Molecular cloning and characterization of a tumor-associated, growth-related, and time-keeping hydroquinone (NADH) oxidase (tNOX) of the HeLa cell surfacePin Ju Chueh
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 41:3732-41. 2002..A single protein with two alternating enzymatic activities indicative of a time-keeping function is unprecedented in the biochemical literature...
Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patientsNaMi Cho
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, West Lafayette, IN 47907, USA
Cancer Immunol Immunother 51:121-9. 2002..Immunocytochemical and histochemical findings showed that mAb 12.1 reacted with the surface membranes of human carcinoma cells and tissues...
Essential role of copper in the activity and regular periodicity of a recombinant, tumor-associated, cell surface, growth-related and time-keeping hydroquinone (NADH) oxidase with protein disulfide-thiol interchange activity (ENOX2)Xiaoyu Tang
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, Hansen Life Sciences Research Building, 201 S University Street, West Lafayette, IN 47907 2064, USA
J Bioenerg Biomembr 42:355-60. 2010..Bound copper emerges as having an essential role in ENOX2 both for enzymatic activity and for the structural changes that underly the periodic alternations in activity that define the time-keeping cycle of the protein...
Alternative splicing as the basis for specific localization of tNOX, a unique hydroquinone (NADH) oxidase, to the cancer cell surfaceXiaoyu Tang
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 46:12337-46. 2007..Taken together, the molecular basis of cancer-cell-specific expression of 34 kDa tNOX appears to reside in the cancer-specific expression of exon 4 minus splice variant mRNA...
Catechin-vanilloid synergies with potential clinical applications in cancerDorothy M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907 2059, USA
Rejuvenation Res 9:45-55. 2006..A catechin-vanilloid mixture where one 350-mg capsule is equivalent to 16 cups of green tea in its ability to inhibit tNOX and growth of cancer cells in culture is undergoing clinical evaluation as a therapeutic aid for cancer patients...
Cell surface NADH oxidases (ECTO-NOX proteins) with roles in cancer, cellular time-keeping, growth, aging and neurodegenerative diseasesD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, West Lafayette, IN, USA
Free Radic Res 37:795-808. 2003..Period length is independent of temperature (temperature compensated) and synchrony is achieved through entrainment...
Specificity of coenzyme Q inhibition of an aging-related cell surface NADH oxidase (ECTO-NOX) that generates superoxideDorothy M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Biofactors 18:33-43. 2003..Inhibition of arNOX by dietary coenzyme Q10 provides a rational basis for dietary coenzyme 10 use to retard aging-related arterial lesions...
Age-related ENOX protein (arNOX) activity correlated with oxidative skin damage in the elderlyDorothy M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
Biofactors 34:237-44. 2008..Topical cosmetic preparations containing substances that block arNOX activity are under evaluation to reduce visible symptoms of skin aging...
Antisense experiments demonstrate an exon 4 minus splice variant mRNA as the basis for expression of tNOX, a cancer-specific cell surface proteinXiaoyu Tang
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Oncol Res 16:557-67. 2007..Growth inhibition was followed by apoptosis in greater than 70% of the transfected cells...
Molecular cloning and characterization of a candidate human growth-related and time-keeping constitutive cell surface hydroquinone (NADH) oxidaseZiying Jiang
Departments of Foods and Nutrition, Biological Sciences, and Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 47:14028-38. 2008..Concentrated solutions of the purified candidate ENOX1 protein irreversibly formed insoluble aggregates, devoid of enzymatic activity, resembling amyloid...
hnRNP F directs formation of an exon 4 minus variant of tumor-associated NADH oxidase (ENOX2)Xiaoyu Tang
NOX Technologies, Inc, Purdue University Research Park, West Lafayette, IN 47906, USA
Mol Cell Biochem 357:55-63. 2011..As MutG75 changed the ESS binding site for hnRNP F, this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2...
Prion proteins and ECTO-NOX proteins exhibit similar oscillating redox activitiesChinpal Kim
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907 2064, USA
Biochem Biophys Res Commun 315:1140-6. 2004....
An aging-related cell surface NADH oxidase (arNOX) generates superoxide and is inhibited by coenzyme QDorothy M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
Mol Cell Biochem 254:101-9. 2003..Additionally, the findings provide a rational basis for the use of dietary coenzyme Q to retard aging-related arterial lesions...
A site-directed mutagenesis analysis of tNOX functional domainsPin Ju Chueh
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biochim Biophys Acta 1594:74-83. 2002..The findings confirm the correctness of the drug and adenine nucleotide binding motifs within the tNOX protein and imply a potential critical role of cysteine residues in determining the period length...
Cell size increased in tissues from transgenic mice overexpressing a cell surface growth-related and cancer-specific hydroquinone oxidase, tNOX, with protein disulfide-thiol interchange activityKader Yagiz
Department of Foods and Nutrition, Purdue University, Stone Hall, 700 W State Street, West Lafayette, Indiana 47907 2059, USA
J Cell Biochem 105:1437-42. 2008..The findings are consistent with the property of tNOX observed in studies with cultured cells as contributing to the enlargement phase of cell growth...
The plasma membrane-associated NADH oxidase (ECTO-NOX) of mouse skin responds to blue lightD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S University Street, West Lafayette, IN 47907 2064, USA
J Photochem Photobiol B 70:7-12. 2003..Addition of melatonin resulted in a new maximum 24 min after melatonin addition. The findings suggest that the ECTO-NOX proteins play a central role in the entrainment of the biological clock both by light and by melatonin...
Response to lithium of a cell surface ECTO-NOX protein with time-keeping characteristicsJessica Kromkowski
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Neurosci Lett 438:121-5. 2008....
Anticancer activity of grape and grape skin extracts alone and combined with green tea infusionsDorothy M Morre
Department of Foods and Nutrition, Purdue University, 700 W State Street, West Lafayette, IN 47907 2059, USA
Cancer Lett 238:202-9. 2006..Intratumoral injections of a 25:1 mixture of a green tea extract plus ground freeze-dried pomace was nearly as effective as standard synergistic green tea-Capsicum mixtures in inhibiting growth of 4T1 mammary tumors in situ in mice...
Downstream targets of altered sphingolipid metabolism in response to inhibition of ENOX2 by phenoxodiolThomas De Luca
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2064, USA
Biofactors 34:253-60. 2008....
arNOX: generator of reactive oxygen species in the skin and sera of aging individuals subject to external modulationDorothy M Morre
NOX Technologies, Inc, West Lafayette, Indiana 47906, USA
Rejuvenation Res 13:162-4. 2010..arNOX activities are highly correlated with values of human skin where a causal relationship is indicated. Ongoing efforts focus on cloning arNOX proteins and development of antiaging formulas based on arNOX inhibition (intervention)...
The plasma membrane NADH oxidase of HeLa cells has hydroquinone oxidase activityT Kishi
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biochim Biophys Acta 1412:66-77. 1999....
Non-mitochondrial coenzyme QD James Morre
NOX Technologies, Purdue Research Park, West Lafayette, IN, USA
Biofactors 37:355-60. 2011....
Aging-related nicotinamide adenine dinucleotide oxidase response to dietary supplementation: the French paradox revisitedD James Morre
NOX Technologies, Inc, West Lafayette, Indiana, USA
Rejuvenation Res 13:159-61. 2010..Among the best sources are certain culinary seasonings, all of which are ingredients used extensively in the French kitchen. Their regular use may contribute to an understanding of the nutritional basis for the French Paradox...
Supplementation with CoQ10 lowers age-related (ar) NOX levels in healthy subjectsDorothy M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
Biofactors 32:221-30. 2008..With all three biofluids, arNOX activity was reduced between 25 and 30% by a 3 x 60 mg daily dose Coenzyme Q10 supplementation. Inhibition was the result of Coenzyme Q10 presence...
Cancer type-specific tNOX isoforms: A putative family of redox protein splice variants with cancer diagnostic and prognostic potentialD James Morre
Department of Medicinal Chemistry and Molecular Pharmacology, HANS Life Sciences Research Building, Purdue University, West Lafayette, IN 47907 2064, USA DJ
Biofactors 34:201-7. 2008....
NADH oxidase activity (NOX) and enlargement of HeLa cells oscillate with two different temperature-compensated period lengths of 22 and 24 minutes corresponding to different NOX formsS Wang
Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
Biochim Biophys Acta 1539:192-204. 2001..The findings demonstrate the presence of a novel oscillating NOX activity at the surface of cancer cells with a period length of 22 min in addition to the constitutive NOX of non-cancer cells and tissues with a period length of 24 min...
Is the cancer protective effect correlated with growth inhibitions by green tea (-)-epigallocatechin gallate mediated through an antioxidant mechanism?H Cutter
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Cancer Lett 162:149-54. 2001..We conclude that it is unlikely that the anticancer action of green tea EGCg on the tNOX protein is mediated through antioxidant properties of EGCg...
Early developmental expression of a normally tumor-associated and drug-inhibited cell surface-located NADH oxidase (ENOX2) in non-cancer cellsNaMi Cho
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, Hansen Life Sciences Research Building, 201 S University Street, West Lafayette, IN 47907, USA
Cancer Immunol Immunother 58:547-52. 2009..The findings suggest that ENOX2 may fulfill some functions essential to the growth of early embryos which are lost in late embryo stages and absent from normal adult cells but which then reappear in cancer...
ECTO-NOX (ENOX) proteins of the cell surface lack thioredoxin reductase activityElena Bosneaga
Department of Medicinal Chemistry and Molecular Pharmacology, HANS Life Sciences Research Building, Purdue University, West Lafayette, IN 47907 2064, USA
Biofactors 34:245-51. 2008..Fibrillation was determined using Thioflavin T fluorescence which paralleled the turbimetric results and the formation of multimers (polymerization) observed on SDS-PAGE...
Plasma membrane NADH oxidase of maize roots responds to gravity and imposed centrifugal forcesE Bacon
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333, USA
Plant Physiol Biochem 39:487-94. 2001..These findings are the first report of a gravity-responsive enzymatic activity of plant roots inhibited by auxin and potentially related to the gravity-induced growth response...
Inhibition of plasma membrane NADH oxidase activity and growth of HeLa cells by natural and synthetic retinoidsS Dai
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Mol Cell Biochem 166:101-9. 1997..The possibility is suggested that inhibition of the plasma membrane NADH oxidase activity by retinoids may be related to their mechanism of inhibition of growth of HeLa cells in culture...
Identification of the major vanilloid component in Capsicum extract by HPLC-EC and HPLC-MSQin Zhou
Bioanalytical Systems Inc, 2701 Kent Avenue, West Lafayette, IN 47906, USA
Phytochem Anal 15:117-20. 2004..Its identity was confirmed by HPLC-MS using a Zorbax SB-CN column with a mobile phase containing formic acid and methanol...
Botanicals for age-related diseases: from field to practiceConnie M Weaver
Foods and Nutrition, Rutgers University, New Brunswick, NJ, USA
Am J Clin Nutr 87:493S-7S. 2008..Botanicals and their relation to bone antiresorptive capacity, cognitive function, vascular effects, and cancer are principal themes in our center...
Surface oxidase and oxidative stress propagation in agingD M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
J Exp Biol 203:1513-21. 2000..The generation of superoxide by NOX forms associated with aging is inhibited by coenzyme Q and provides a rational basis for the anti-aging activity of circulating coenzyme Q...
The sulfonylurea-inhibited NADH oxidase activity of HeLa cell plasma membranes has properties of a protein disulfide-thiol oxidoreductase with protein disulfide-thiol interchange activityD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
J Bioenerg Biomembr 30:477-87. 1998..Rather, it may carry out some other role more closely related to a function in growth, such as protein disulfide-thiol interchange coupled to cell enlargement...
Aqueous two-phase partition applied to the isolation of plasma membranes and Golgi apparatus from cultured mammalian cellsD M Morre
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
J Chromatogr B Biomed Sci Appl 743:377-87. 2000..Also described is a periodic NADH oxidase, a new enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions for measurement of activity...
Isolation and characterization of a tumor-associated NADH oxidase (tNOX) from the HeLa cell surfaceF Yantiri
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 1333 Hansen Life Sciences Research Building, West Lafayette, Indiana 47907 1333, USA
Arch Biochem Biophys 391:149-59. 2001..The tNOX form from the surface of HeLa cells yielded N-terminal sequence consistent with a coidentity of the cell surface and serum forms of the two activities...
Electron donation to the plasma membrane redox system of cultured carrot cells stimulates proton releaseR Barr
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Biochim Biophys Acta 1017:91-5. 1990..This behavior by cultured carrot cells indicates that a certain threshold of proton concentration in a limited membrane domain must be reached in order for the proton channel to be opened...
Mode of action of the anticancer quassinoids--inhibition of the plasma membrane NADH oxidaseD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Life Sci 63:595-604. 1998....
Differential response of the NADH oxidase of plasma membranes of rat liver and hepatoma and HeLa cells to thiol reagentsD J Morre
Department of Medicinal Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
J Bioenerg Biomembr 27:137-44. 1995..1992). Results are consistent with a structural modification of a NADH oxidase activity involving thiol groups present in plasma membranes of rat hepatoma and HeLa cells but absent or inaccessible with plasma membranes of rat liver...
Antitumor sulfonylurea-inhibited NADH oxidase of cultured HeLa cells shed into mediaD J Morre
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
Biochim Biophys Acta 1280:197-206. 1996..5 kDa drug-inhibited NADH oxidase activity of the culture media being a shed form of the corresponding native 34 kDa antitumor sulfonylurea-inhibited NADH oxidase activity of the HeLa cell plasma membrane...
Cancer isoform of a tumor-associated cell surface NADH oxidase (tNOX) has properties of a prionM Kelker
Department of Medicinal Chemistry and Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 40:7351-4. 2001..This property, that of converting the normal form of a protein into a likeness of itself, is one of the defining characteristics of the group of proteins designated as prions...
Isolation of renal brush bordersD James Morre
Purdue University, West Lafayette, Indiana, USA
Curr Protoc Cell Biol . 2007..Marker enzymes and morphological parameters are included for assessment of yield and fraction purity...
NADH oxidase activity from sera altered by capsaicin is widely distributed among cancer patientsD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Arch Biochem Biophys 342:224-30. 1997..The capsaicin-inhibited activity was found in sera over a broad spectrum of cancer patients including patients with solid cancers (e.g., breast, prostate, lung, ovarian) as well as with leukemias and lymphomas...
Effect of the quassinoids glaucarubolone and simalikalactone D on growth of cells permanently infected with feline and human immunodeficiency viruses and on viral infectionsD J Morre
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
Life Sci 62:213-9. 1998..In addition the quassinoids may delay viral infection suggesting some commonality between the mechanism responsible for inhibition of the growth of the transformed phenotype and viral infection...
