A G Tomasselli

Summary

Affiliation: Pfizer Global Research and Development
Country: USA

Publications

  1. ncbi Changes in gamma-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1
    Donald B Carter
    Global Research and Development, Pfizer Inc, Kalamazoo, MI 49001, USA
    Mol Neurodegener 3:6. 2008
  2. ncbi High yield expression of human BACE constructs in Eschericia coli for refolding, purification, and high resolution diffracting crystal forms
    A G Tomasselli
    Global Research and Development, St Louis Laboratories, Pfizer Inc, Chesterfield, MO 63017, USA
    Protein Pept Lett 15:131-43. 2008
  3. ncbi Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction
    T L Emmons
    Global Research and Development, St Louis Laboratories, Pfizer Inc, Chesterfield, MO 63017, USA
    Protein Pept Lett 15:119-30. 2008
  4. ncbi Large scale refolding and purification of the catalytic domain of human BACE-2 produced in E. coli
    T L Emmons
    Global Research and Development, St Louis Laboratories, Pfizer Inc, 700 Chesterfield Parkway West, Chesterfield, MO, USA
    Protein Pept Lett 16:121-31. 2009
  5. ncbi Production of chemokines CTAPIII and NAP/2 by digestion of recombinant ubiquitin-CTAPIII with yeast ubiquitin C-terminal hydrolase and human immunodeficiency virus protease
    A M Mildner
    Protein Science, Cell and Molecular Biology, Pharmacia and Upjohn, 301 Henrietta Street, Kalamazoo, Michigan 49007, USA
    Protein Expr Purif 16:347-54. 1999
  6. ncbi [W206R]-procaspase 3: an inactivatable substrate for caspase 8
    K B Rank
    Pharmacia Corporation, Kalamazoo, Michigan 49007, USA
    Protein Expr Purif 22:258-66. 2001
  7. ncbi Prediction of the tertiary structure of a caspase-9/inhibitor complex
    K C Chou
    Computer Aided Drug Discovery, Pharmacia and Upjohn, Kalamazoo, MI 49007 4940, USA
    FEBS Lett 470:249-56. 2000
  8. ncbi Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity
    R Yan
    Cell and Molecular Biology, Pharmacia and Upjohn, Inc, Kalamazoo, MI 49007, USA
    Nature 402:533-7. 1999
  9. ncbi HIV-1 protease cleaves actin during acute infection of human T-lymphocytes
    L D Adams
    Biochemistry Unit, Upjohn Laboratories, Kalamazoo, MI 49001
    AIDS Res Hum Retroviruses 8:291-5. 1992
  10. ncbi Calcium-free calmodulin is a substrate of proteases from human immunodeficiency viruses 1 and 2
    A G Tomasselli
    Biochemistry Unit, Upjohn Company, Kalamazoo, Michigan 49001
    Proteins 10:1-9. 1991

Collaborators

Detail Information

Publications11

  1. ncbi Changes in gamma-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1
    Donald B Carter
    Global Research and Development, Pfizer Inc, Kalamazoo, MI 49001, USA
    Mol Neurodegener 3:6. 2008
    ..This study provides an important structural clue for the rational design of drugs to inhibit processing of APP at the gamma-site without interfering with Notch processing...
  2. ncbi High yield expression of human BACE constructs in Eschericia coli for refolding, purification, and high resolution diffracting crystal forms
    A G Tomasselli
    Global Research and Development, St Louis Laboratories, Pfizer Inc, Chesterfield, MO 63017, USA
    Protein Pept Lett 15:131-43. 2008
    ..In addition, a crystal form that does not require the presence of an inhibitor has been obtained for pQE70-BACE. This ligand-free crystal form has proven useful for the preparation of BACE-inhibitor complexes in soaking experiments...
  3. ncbi Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction
    T L Emmons
    Global Research and Development, St Louis Laboratories, Pfizer Inc, Chesterfield, MO 63017, USA
    Protein Pept Lett 15:119-30. 2008
    ..ES(432) (His)(6) derivative that was purified yielding an enzyme that was no more active than untreated BACE but co-crystallized with inhibitors producing well shaped, bipyramidal co-crystals diffracting to 2.6 A resolution...
  4. ncbi Large scale refolding and purification of the catalytic domain of human BACE-2 produced in E. coli
    T L Emmons
    Global Research and Development, St Louis Laboratories, Pfizer Inc, 700 Chesterfield Parkway West, Chesterfield, MO, USA
    Protein Pept Lett 16:121-31. 2009
    ..Refolded BACE-2 was purified by anion exchange, molecular sieve, and affinity chromatographies, yielding 105 mg of homogeneous enzyme (kcat/ Km = 1.2 x 10(4) x M(-1) x sec(-1)) from 8 liters of E. coli cell culture...
  5. ncbi Production of chemokines CTAPIII and NAP/2 by digestion of recombinant ubiquitin-CTAPIII with yeast ubiquitin C-terminal hydrolase and human immunodeficiency virus protease
    A M Mildner
    Protein Science, Cell and Molecular Biology, Pharmacia and Upjohn, 301 Henrietta Street, Kalamazoo, Michigan 49007, USA
    Protein Expr Purif 16:347-54. 1999
    ..This strategy, which employs YUH1 and the HIV-1 Pr as tools for the highly selective cleavage of the chimeric substrate, should be applicable to the large-scale production of a variety of peptides...
  6. ncbi [W206R]-procaspase 3: an inactivatable substrate for caspase 8
    K B Rank
    Pharmacia Corporation, Kalamazoo, Michigan 49007, USA
    Protein Expr Purif 22:258-66. 2001
    ....
  7. ncbi Prediction of the tertiary structure of a caspase-9/inhibitor complex
    K C Chou
    Computer Aided Drug Discovery, Pharmacia and Upjohn, Kalamazoo, MI 49007 4940, USA
    FEBS Lett 470:249-56. 2000
    ..The predicted structure of caspase-9 can serve as a reference for subsite analysis relative to rational design of highly selective caspase inhibitors for therapeutic application...
  8. ncbi Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity
    R Yan
    Cell and Molecular Biology, Pharmacia and Upjohn, Inc, Kalamazoo, MI 49007, USA
    Nature 402:533-7. 1999
    ..Thus, Asp2 is a new protein target for drugs that are designed to block the production of amyloid beta-peptide peptide and the consequent formation of amyloid plaque in Alzheimer's disease...
  9. ncbi HIV-1 protease cleaves actin during acute infection of human T-lymphocytes
    L D Adams
    Biochemistry Unit, Upjohn Laboratories, Kalamazoo, MI 49001
    AIDS Res Hum Retroviruses 8:291-5. 1992
    ..Hydrolysis by the HIV-1 protease of physiologically important host cellular proteins during infection may have important consequences relative to viral pathogenesis...
  10. ncbi Calcium-free calmodulin is a substrate of proteases from human immunodeficiency viruses 1 and 2
    A G Tomasselli
    Biochemistry Unit, Upjohn Company, Kalamazoo, Michigan 49001
    Proteins 10:1-9. 1991
    ..abstract truncated at 250 words)..
  11. ncbi Actin, troponin C, Alzheimer amyloid precursor protein and pro-interleukin 1 beta as substrates of the protease from human immunodeficiency virus
    A G Tomasselli
    Upjohn Laboratories, Upjohn Company, Kalamazoo, Michigan 49001
    J Biol Chem 266:14548-53. 1991
    ....