Hendrik Neubert

Summary

Affiliation: Pfizer Global Research and Development
Country: USA

Publications

  1. ncbi Assessing immunogenicity in the presence of excess protein therapeutic using immunoprecipitation and quantitative mass spectrometry
    Hendrik Neubert
    Pfizer Global Research and Development, Sandwich, UK
    Anal Chem 80:6907-14. 2008
  2. ncbi Label-free detection of differential protein expression by LC/MALDI mass spectrometry
    Hendrik Neubert
    Pfizer Global Research and Development, Sandwich, Kent CT13 9NJ, United Kingdom
    J Proteome Res 7:2270-9. 2008
  3. ncbi Online capillary weak cation exchange enrichment hyphenated to nanospray mass spectrometry for quantitation of a basic pegvisomant derived peptide
    Hendrik Neubert
    Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent, UK
    J Chromatogr A 1216:6151-4. 2009
  4. ncbi Online high-flow peptide immunoaffinity enrichment and nanoflow LC-MS/MS: assay development for total salivary pepsin/pepsinogen
    Hendrik Neubert
    Pharmacokinetics Dynamics and Metabolism and Biotherapeutics and Translational Research, Pfizer Limited, Kent, UK
    Clin Chem 56:1413-23. 2010
  5. ncbi Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
    David Olivier D Azulay
    Pfizer Global Research and Development, Sandwich, UK
    BMC Bioinformatics 11:371. 2010
  6. ncbi An immunoaffinity liquid chromatography-tandem mass spectrometry assay for the quantitation of matrix metalloproteinase 9 in mouse serum
    Mireia Fernández Ocaña
    Experimental Biological Sciences, Pfizer, Kent CT13 9NJ, UK
    Anal Biochem 399:202-10. 2010
  7. ncbi Widespread sulfenic acid formation in tissues in response to hydrogen peroxide
    Adrian T Saurin
    Department of Cardiology, Cardiovascular Division, The Rayne Institute, St. Thomas' Hospital, King's College London, London SE1 7EH, United Kingdom
    Proc Natl Acad Sci U S A 101:17982-7. 2004
  8. ncbi Identification of placental transforming growth factor-beta and bikunin metabolites as contaminants of pharmaceutical human chorionic gonadotrophin preparations by proteomic techniques
    Sotiris Malatos
    Biomedical Sciences, Institute of Social and Health Research, Middlesex University, Enfield EN3 4SA, United Kingdom
    Mol Cell Proteomics 4:984-92. 2005
  9. ncbi Alpha tocopherol supplementation elevates plasma apolipoprotein A1 isoforms in normal healthy subjects
    Sarah Aldred
    Life and Health Sciences, Aston University, Birmingham, UK
    Proteomics 6:1695-703. 2006

Collaborators

  • P Eaton
  • H R Griffiths
  • Mireia Fernández Ocaña
  • David Olivier D Azulay
  • Sarah Aldred
  • Sotiris Malatos
  • Adrian T Saurin
  • Ian Mudway
  • Frank J Kelly
  • Tommaso Sozzi
  • Melissa M Grant
  • Andrew T Kicman
  • Ray K Iles
  • Jonathan P Brennan

Detail Information

Publications9

  1. ncbi Assessing immunogenicity in the presence of excess protein therapeutic using immunoprecipitation and quantitative mass spectrometry
    Hendrik Neubert
    Pfizer Global Research and Development, Sandwich, UK
    Anal Chem 80:6907-14. 2008
    ..This approach complements established methodologies for the assessment of immunogenicity responses and currently supports clinical programs addressing the safety and tolerability of human growth hormone analogues...
  2. ncbi Label-free detection of differential protein expression by LC/MALDI mass spectrometry
    Hendrik Neubert
    Pfizer Global Research and Development, Sandwich, Kent CT13 9NJ, United Kingdom
    J Proteome Res 7:2270-9. 2008
    ..01), which represented candidates for targeted peptide identification using MS/MS. Biologically interpretable protein identifications that could be derived underpin the general utility of this label-free LC/MALDI strategy...
  3. ncbi Online capillary weak cation exchange enrichment hyphenated to nanospray mass spectrometry for quantitation of a basic pegvisomant derived peptide
    Hendrik Neubert
    Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent, UK
    J Chromatogr A 1216:6151-4. 2009
    ..Such a LC configuration including the use of other sorbents such as strong cation exchange media is proposed as an enabling tool in assay development for quantitative protein bioanalysis by LC-MS/MS...
  4. ncbi Online high-flow peptide immunoaffinity enrichment and nanoflow LC-MS/MS: assay development for total salivary pepsin/pepsinogen
    Hendrik Neubert
    Pharmacokinetics Dynamics and Metabolism and Biotherapeutics and Translational Research, Pfizer Limited, Kent, UK
    Clin Chem 56:1413-23. 2010
    ..As a proof of principle, we describe a novel online immunoaffinity LC-MS/MS configuration that combines high-flow peptide immunoaffinity enrichment and nanoflow LC-MS/MS...
  5. ncbi Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
    David Olivier D Azulay
    Pfizer Global Research and Development, Sandwich, UK
    BMC Bioinformatics 11:371. 2010
    ..As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality...
  6. ncbi An immunoaffinity liquid chromatography-tandem mass spectrometry assay for the quantitation of matrix metalloproteinase 9 in mouse serum
    Mireia Fernández Ocaña
    Experimental Biological Sciences, Pfizer, Kent CT13 9NJ, UK
    Anal Biochem 399:202-10. 2010
    ..03 to 7.3nM mouse MMP-9. Finally, the method was employed to measure MMP-9 concentrations in 30 naïve mouse serum samples, and results were compared with those obtained by an immunoassay...
  7. ncbi Widespread sulfenic acid formation in tissues in response to hydrogen peroxide
    Adrian T Saurin
    Department of Cardiology, Cardiovascular Division, The Rayne Institute, St. Thomas' Hospital, King's College London, London SE1 7EH, United Kingdom
    Proc Natl Acad Sci U S A 101:17982-7. 2004
    ..These approaches may find widespread utility in the study of oxidative stress, particularly because hydrogen peroxide is used extensively in models of disease or redox signaling...
  8. ncbi Identification of placental transforming growth factor-beta and bikunin metabolites as contaminants of pharmaceutical human chorionic gonadotrophin preparations by proteomic techniques
    Sotiris Malatos
    Biomedical Sciences, Institute of Social and Health Research, Middlesex University, Enfield EN3 4SA, United Kingdom
    Mol Cell Proteomics 4:984-92. 2005
    ..In combination these molecules may be responsible for the anti-human immunodeficiency virus-associated Kaposi's sarcoma activity demonstrated for these urinary human chorionic gonadotrophin preparations...
  9. ncbi Alpha tocopherol supplementation elevates plasma apolipoprotein A1 isoforms in normal healthy subjects
    Sarah Aldred
    Life and Health Sciences, Aston University, Birmingham, UK
    Proteomics 6:1695-703. 2006
    ..01 after 28 days supplementation with 268 mg AT/day). These data demonstrate the time and dose sensitivity of the plasma proteome to AT supplementation...